MASCOT Search Results

Protein View: A4_HUMAN

Amyloid beta A4 protein OS=Homo sapiens GN=APP PE=1 SV=3

Database: SwissProt
Score: 4076
Nominal mass (Mr): 87914
Calculated pI: 4.73
Taxonomy: Homo sapiens

Sequence similarity is available as an NCBI BLAST search of A4_HUMAN against nr.

Search parameters

MS data file: zengli_20160316_1_2.msm
Enzyme:

Trypsin: cuts C-term side of KR unless next residue is P.

Fixed modifications: Carbamidomethyl (C)
Variable modifications: Phospho (ST), Oxidation (M)

Protein sequence coverage: 42%

Matched peptides shown in bold red.

1 MLPGLALLLL AAWTARALEV PTDGNAGLLA EPQIAMFCGR LNMHMNVQNG
51 KWDSDPSGTK TCIDTKEGIL QYCQEVYPEL QITNVVEANQ PVTIQNWCKR
101 GRKQCKTHPH FVIPYRCLVG EFVSDALLVP DKCKFLHQER MDVCETHLHW
151 HTVAKETCSE KSTNLHDYGM LLPCGIDKFR GVEFVCCPLA EESDNVDSAD
201 AEEDDSDVWW GGADTDYADG SEDKVVEVAE EEEVAEVEEE EADDDEDDED
251 GDEVEEEAEE PYEEATERTT SIATTTTTTT ESVEEVVREV CSEQAETGPC
301 RAMISRWYFD VTEGKCAPFF YGGCGGNRNN FDTEEYCMAV CGSAMSQSLL
351 KTTQEPLARD PVKLPTTAAS TPDAVDKYLE TPGDENEHAH FQKAKERLEA
401 KHRERMSQVM REWEEAERQA KNLPKADKKA VIQHFQEKVE SLEQEAANER
451 QQLVETHMAR VEAMLNDRRR LALENYITAL QAVPPRPRHV FNMLKKYVRA
501 EQKDRQHTLK HFEHVRMVDP KKAAQIRSQV MTHLRVIYER MNQSLSLLYN
551 VPAVAEEIQD EVDELLQKEQ NYSDDVLANM ISEPRISYGN DALMPSLTET
601 KTTVELLPVN GEFSLDDLQP WHSFGADSVP ANTENEVEPV DARPAADRGL
651 TTRPGSGLTN IKTEEISEVK MDAEFRHDSG YEVHHQKLVF FAEDVGSNKG
701 AIIGLMVGGV VIATVIVITL VMLKKKQYTS IHHGVVEVDA AVTPEERHLS
751 KMQQNGYENP TYKFFEQMQN

Unformatted sequence string: 770 residues (for pasting into other applications).

Query Start End Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
11619 41 51 439.8727 1316.5964 1316.5965 -0.10 0 47 5e-05 1Score > 22 indicates identity
Score > 16 indicates homology
U R.LNMHMNVQNGK.W + 2 Oxidation (M)
11620 41 51 659.3060 1316.5975 1316.5965 0.75 0 41 0.00019 1Score > 22 indicates identity
Score > 17 indicates homology
U R.LNMHMNVQNGK.W + 2 Oxidation (M)
19803 41 60 573.5103 2290.0119 2290.0107 0.55 1 16 0.031 1Score > 22 indicates identity
Score > 14 indicates homology
U R.LNMHMNVQNGKWDSDPSGTK.T + 2 Oxidation (M)
7724 52 60 496.7197 991.4248 991.4247 0.12 0 23 0.012 1Score > 20 indicates identity
Score > 16 indicates homology
U K.WDSDPSGTK.T
11053 107 116 422.8965 1265.6677 1265.6669 0.62 0 32 0.00098 1Score > 20 indicates identity
Score > 15 indicates homology
U K.THPHFVIPYR.C
11054 107 116 422.8965 1265.6677 1265.6669 0.64 0 21 0.012 1Score > 20 indicates identity
Score > 14 indicates homology
U K.THPHFVIPYR.C
11055 107 116 633.8412 1265.6678 1265.6669 0.68 0 27 0.003 1Score > 20 indicates identity
Score > 14 indicates homology
U K.THPHFVIPYR.C
11056 107 116 422.8967 1265.6682 1265.6669 1.03 0 28 0.0023 1Score > 20 indicates identity
Score > 14 indicates homology
U K.THPHFVIPYR.C
18551 269 288 1064.0365 2126.0584 2126.0591 -0.29 0 102 2.6e-10 1Score > 21 indicates identity
Score > 19 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18552 269 288 1064.0370 2126.0595 2126.0591 0.22 0 25 0.0046 1Score > 21 indicates identity
Score > 14 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18553 269 288 1064.0372 2126.0598 2126.0591 0.34 0 84 1.3e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18554 269 288 1064.0372 2126.0598 2126.0591 0.35 0 85 1e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18555 269 288 1064.0372 2126.0599 2126.0591 0.39 0 94 1.5e-09 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18556 269 288 1064.0373 2126.0600 2126.0591 0.42 0 76 7.8e-08 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18557 269 288 1064.0373 2126.0600 2126.0591 0.44 0 43 9.6e-05 1Score > 21 indicates identity
Score > 15 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18558 269 288 709.6940 2126.0601 2126.0591 0.47 0 13 0.054 1Score > 21 indicates identity
Score > 13 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18559 269 288 709.6940 2126.0603 2126.0591 0.56 0 49 2.7e-05 1Score > 21 indicates identity
Score > 16 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18560 269 288 1064.0374 2126.0603 2126.0591 0.58 0 70 2.6e-07 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18561 269 288 1064.0374 2126.0603 2126.0591 0.59 0 112 2.8e-11 1Score > 21 indicates identity
Score > 20 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18562 269 288 1064.0374 2126.0603 2126.0591 0.59 0 101 3.7e-10 1Score > 21 indicates identity
Score > 19 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18563 269 288 1064.0374 2126.0603 2126.0591 0.60 0 78 4.4e-08 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18564 269 288 1064.0375 2126.0605 2126.0591 0.69 0 79 3.9e-08 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18565 269 288 1064.0376 2126.0606 2126.0591 0.70 0 80 3.1e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18566 269 288 1064.0376 2126.0606 2126.0591 0.70 0 82 2.3e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18567 269 288 1064.0376 2126.0606 2126.0591 0.70 0 65 8.1e-07 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18568 269 288 1064.0376 2126.0606 2126.0591 0.71 0 82 4e-08 1Score > 21 indicates identity
Score > 20 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18569 269 288 1064.0376 2126.0606 2126.0591 0.72 0 82 3.6e-08 1Score > 21 indicates identity
Score > 21 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18570 269 288 1064.0376 2126.0606 2126.0591 0.73 0 28 0.0024 1Score > 21 indicates identity
Score > 14 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18571 269 288 1064.0376 2126.0606 2126.0591 0.74 0 88 5.6e-09 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18572 269 288 1064.0376 2126.0606 2126.0591 0.74 0 76 7.6e-08 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18573 269 288 1064.0377 2126.0608 2126.0591 0.82 0 29 0.0019 1Score > 21 indicates identity
Score > 14 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18574 269 288 1064.0378 2126.0610 2126.0591 0.89 0 95 2.5e-09 1Score > 21 indicates identity U R.TTSIATTTTTTTESVEEVVR.E
18575 269 288 1064.0378 2126.0610 2126.0591 0.93 0 67 5.7e-07 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18576 269 288 709.6943 2126.0612 2126.0591 1.00 0 19 0.017 1Score > 21 indicates identity
Score > 14 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18577 269 288 1064.0379 2126.0612 2126.0591 1.03 0 56 6.2e-06 1Score > 21 indicates identity
Score > 16 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18578 269 288 1064.0379 2126.0613 2126.0591 1.03 0 62 1.6e-06 1Score > 21 indicates identity
Score > 16 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18579 269 288 1064.0379 2126.0613 2126.0591 1.04 0 52 1.2e-05 1Score > 21 indicates identity
Score > 16 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18580 269 288 1064.0379 2126.0613 2126.0591 1.05 0 77 6.4e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18581 269 288 1064.0379 2126.0613 2126.0591 1.07 0 120 7.9e-12 1Score > 21 indicates identity U R.TTSIATTTTTTTESVEEVVR.E
18582 269 288 1064.0380 2126.0615 2126.0591 1.12 0 88 1.2e-08 1Score > 21 indicates identity U R.TTSIATTTTTTTESVEEVVR.E
18583 269 288 709.6945 2126.0615 2126.0591 1.16 0 38 0.00025 1Score > 21 indicates identity
Score > 15 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18584 269 288 709.6946 2126.0619 2126.0591 1.34 0 81 3e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18585 269 288 1064.0383 2126.0621 2126.0591 1.41 0 69 3.6e-07 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18586 269 288 709.6950 2126.0633 2126.0591 1.97 0 28 0.0025 1Score > 21 indicates identity
Score > 14 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18587 269 288 1064.0392 2126.0638 2126.0591 2.22 0 76 7.1e-08 1Score > 21 indicates identity
Score > 17 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
18588 269 288 1064.0392 2126.0638 2126.0591 2.23 0 25 0.0049 1Score > 21 indicates identity
Score > 14 indicates homology
U R.TTSIATTTTTTTESVEEVVR.E
15105 302 315 573.6118 1717.8137 1717.8134 0.17 1 24 0.0055 1Score > 22 indicates identity
Score > 14 indicates homology
U R.AMISRWYFDVTEGK.C + Oxidation (M)
9728 307 315 572.7694 1143.5243 1143.5237 0.51 0 13 0.055 1Score > 21 indicates identity
Score > 13 indicates homology
U R.WYFDVTEGK.C
9729 307 315 572.7694 1143.5243 1143.5237 0.55 0 34 0.0015 1Score > 21 indicates identity
Score > 19 indicates homology
U R.WYFDVTEGK.C
16669 378 393 638.9584 1913.8535 1913.8544 -0.48 0 6 0.26 1Score > 22 indicates identity
Score > 13 indicates homology
U K.YLETPGDENEHAHFQK.A
16670 378 393 479.4711 1913.8552 1913.8544 0.42 0 14 0.051 1Score > 22 indicates identity
Score > 13 indicates homology
U K.YLETPGDENEHAHFQK.A
16671 378 393 957.9349 1913.8553 1913.8544 0.50 0 100 4.3e-10 1Score > 22 indicates identity
Score > 19 indicates homology
U K.YLETPGDENEHAHFQK.A
16672 378 393 479.4716 1913.8573 1913.8544 1.51 0 16 0.033 1Score > 23 indicates identity
Score > 13 indicates homology
U K.YLETPGDENEHAHFQK.A
18436 378 395 705.3361 2112.9864 2112.9864 -0.031 1 8 0.19 1Score > 22 indicates identity
Score > 13 indicates homology
U K.YLETPGDENEHAHFQKAK.E
15059 406 418 571.5838 1711.7294 1711.7294 0.036 1 24 0.0053 1Score > 21 indicates identity
Score > 14 indicates homology
U R.MSQVMREWEEAER.Q + 2 Oxidation (M)
15060 406 418 856.8721 1711.7296 1711.7294 0.16 1 12 0.29 1Score > 21 indicates identity
Score > 19 indicates homology
U R.MSQVMREWEEAER.Q + 2 Oxidation (M)
15461 406 418 880.8657 1759.7168 1759.7059 6.22 1 3 2 4Score > 21 indicates identity
Score > 19 indicates homology
U R.MSQVMREWEEAER.Q + Phospho (ST)
15750 406 418 896.8528 1791.6911 1791.6957 -2.55 1 5 1.4 1Score > 19 indicates identity U R.MSQVMREWEEAER.Q + Phospho (ST); 2 Oxidation (M)
7301 412 418 474.7064 947.3983 947.3984 -0.18 0 34 0.002 1Score > 20 indicates identity U R.EWEEAER.Q
5443 419 425 399.7453 797.4760 797.4759 0.15 1 23 0.013 1Score > 17 indicates identity U R.QAKNLPK.A
5254 422 428 393.2294 784.4443 784.4443 0.088 1 31 0.0024 1Score > 17 indicates identity U K.NLPKADK.K
10660 429 438 409.9001 1226.6785 1226.6771 1.12 1 45 0.00012 1Score > 19 indicates identity U K.KAVIQHFQEK.V
10661 429 438 614.3467 1226.6788 1226.6771 1.39 1 59 5.2e-06 1Score > 19 indicates identity U K.KAVIQHFQEK.V
9166 430 438 367.2013 1098.5821 1098.5822 -0.083 0 20 0.012 1Score > 20 indicates identity
Score > 14 indicates homology
U K.AVIQHFQEK.V
9167 430 438 550.2986 1098.5826 1098.5822 0.38 0 35 0.00071 1Score > 20 indicates identity
Score > 16 indicates homology
U K.AVIQHFQEK.V
21003 430 450 614.5580 2454.2031 2454.2139 -4.40 1 1 0.99 1Score > 21 indicates identity
Score > 13 indicates homology
U K.AVIQHFQEKVESLEQEAANER.Q
21004 430 450 819.0791 2454.2156 2454.2139 0.70 1 46 5.1e-05 1Score > 21 indicates identity
Score > 15 indicates homology
U K.AVIQHFQEKVESLEQEAANER.Q
21005 430 450 614.5618 2454.2180 2454.2139 1.69 1 2 0.7 1Score > 20 indicates identity
Score > 13 indicates homology
U K.AVIQHFQEKVESLEQEAANER.Q
12280 439 450 687.8286 1373.6427 1373.6422 0.33 0 66 1.7e-06 1Score > 22 indicates identity
Score > 20 indicates homology
U K.VESLEQEAANER.Q
21642 439 460 646.8160 2583.2349 2583.2347 0.068 1 6 0.25 1Score > 21 indicates identity
Score > 13 indicates homology
U K.VESLEQEAANERQQLVETHMAR.V + Oxidation (M)
21643 439 460 862.0861 2583.2366 2583.2347 0.74 1 50 2.2e-05 1Score > 21 indicates identity
Score > 16 indicates homology
U K.VESLEQEAANERQQLVETHMAR.V + Oxidation (M)
21644 439 460 862.0864 2583.2374 2583.2347 1.07 1 7 0.23 1Score > 21 indicates identity
Score > 13 indicates homology
U K.VESLEQEAANERQQLVETHMAR.V + Oxidation (M)
10673 451 460 410.2085 1227.6036 1227.6030 0.49 0 6 0.25 1Score > 22 indicates identity
Score > 13 indicates homology
U R.QQLVETHMAR.V + Oxidation (M)
10674 451 460 614.8091 1227.6037 1227.6030 0.57 0 58 1.5e-05 1Score > 22 indicates identity U R.QQLVETHMAR.V + Oxidation (M)
18945 451 468 725.0224 2172.0452 2172.0415 1.70 1 7 0.2 1Score > 22 indicates identity
Score > 13 indicates homology
U R.QQLVETHMARVEAMLNDR.R + 2 Oxidation (M)
7422 461 468 482.2328 962.4511 962.4491 2.05 0 46 9.7e-05 1Score > 21 indicates identity
Score > 19 indicates homology
U R.VEAMLNDR.R + Oxidation (M)
9416 461 469 560.2822 1118.5499 1118.5502 -0.32 1 35 0.0025 1Score > 22 indicates identity U R.VEAMLNDRR.R + Oxidation (M)
18988 470 488 545.3183 2177.2442 2177.2433 0.42 1 16 0.026 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
18989 470 488 545.3183 2177.2442 2177.2433 0.42 1 28 0.0017 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
18990 470 488 726.7556 2177.2449 2177.2433 0.75 1 8 0.16 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
18991 470 488 726.7558 2177.2454 2177.2433 1.01 1 23 0.005 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
18992 470 488 726.7560 2177.2462 2177.2433 1.33 1 20 0.0094 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
18993 470 488 545.3190 2177.2471 2177.2433 1.76 1 35 0.00033 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
18994 470 488 545.3192 2177.2475 2177.2433 1.96 1 24 0.0041 1Score > 13 indicates identity U R.RLALENYITALQAVPPRPR.H
17548 471 488 506.2926 2021.1414 2021.1422 -0.40 0 50 1.1e-05 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17550 471 488 1011.5784 2021.1422 2021.1422 0.012 0 35 0.00037 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17551 471 488 674.7214 2021.1425 2021.1422 0.18 0 14 0.046 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17552 471 488 506.2929 2021.1425 2021.1422 0.19 0 54 4.2e-06 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17553 471 488 1011.5786 2021.1426 2021.1422 0.23 0 26 0.0027 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17554 471 488 1011.5786 2021.1427 2021.1422 0.27 0 67 2e-07 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17555 471 488 674.7216 2021.1429 2021.1422 0.37 0 5 0.34 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17556 471 488 674.7216 2021.1429 2021.1422 0.39 0 33 0.00059 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17557 471 488 674.7217 2021.1432 2021.1422 0.50 0 8 0.16 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17558 471 488 1011.5791 2021.1436 2021.1422 0.74 0 26 0.0026 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17560 471 488 674.7220 2021.1442 2021.1422 1.03 0 17 0.021 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17561 471 488 674.7221 2021.1445 2021.1422 1.14 0 43 5.2e-05 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17562 471 488 674.7221 2021.1445 2021.1422 1.16 0 2 0.7 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17563 471 488 674.7225 2021.1457 2021.1422 1.76 0 53 4.6e-06 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
17565 471 488 674.7230 2021.1472 2021.1422 2.50 0 41 7.2e-05 1Score > 13 indicates identity U R.LALENYITALQAVPPRPR.H
6757 489 495 452.7391 903.4636 903.4637 -0.062 0 13 0.063 1Score > 23 indicates identity
Score > 13 indicates homology
U R.HVFNMLK.K + Oxidation (M)
6758 489 495 452.7393 903.4640 903.4637 0.34 0 26 0.012 1Score > 23 indicates identity
Score > 19 indicates homology
U R.HVFNMLK.K + Oxidation (M)
8219 489 496 516.7866 1031.5587 1031.5586 0.051 1 32 0.0047 1Score > 22 indicates identity
Score > 21 indicates homology
U R.HVFNMLKK.Y + Oxidation (M)
12974 506 516 358.6955 1430.7529 1430.7531 -0.12 1 16 0.031 1Score > 21 indicates identity
Score > 14 indicates homology
U R.QHTLKHFEHVR.M
12975 506 516 358.6955 1430.7529 1430.7531 -0.12 1 32 0.00093 1Score > 21 indicates identity
Score > 15 indicates homology
U R.QHTLKHFEHVR.M
12976 506 516 477.9250 1430.7532 1430.7531 0.094 1 10 0.16 1Score > 21 indicates identity
Score > 14 indicates homology
U R.QHTLKHFEHVR.M
12977 506 516 716.3848 1430.7551 1430.7531 1.39 1 43 0.00012 1Score > 21 indicates identity
Score > 16 indicates homology
U R.QHTLKHFEHVR.M
13921 523 535 509.6130 1525.8172 1525.8147 1.64 1 5 1.5 1Score > 19 indicates identity U K.AAQIRSQVMTHLR.V + Oxidation (M)
7663 528 535 494.2557 986.4969 986.4968 0.14 0 20 0.015 1Score > 22 indicates identity
Score > 14 indicates homology
U R.SQVMTHLR.V + Oxidation (M)
17316 569 585 998.9504 1995.8862 1995.8844 0.91 0 97 7.8e-10 1Score > 22 indicates identity
Score > 19 indicates homology
U K.EQNYSDDVLANMISEPR.I + Oxidation (M)
17317 569 585 998.9504 1995.8862 1995.8844 0.91 0 134 3e-13 1Score > 22 indicates identity
Score > 21 indicates homology
U K.EQNYSDDVLANMISEPR.I + Oxidation (M)
17318 569 585 998.9504 1995.8863 1995.8844 0.96 0 110 5.1e-11 1Score > 22 indicates identity
Score > 19 indicates homology
U K.EQNYSDDVLANMISEPR.I + Oxidation (M)
17319 569 585 998.9504 1995.8863 1995.8844 0.96 0 117 9.9e-12 1Score > 22 indicates identity
Score > 20 indicates homology
U K.EQNYSDDVLANMISEPR.I + Oxidation (M)
17320 569 585 666.3029 1995.8869 1995.8844 1.27 0 38 0.0003 1Score > 22 indicates identity
Score > 15 indicates homology
U K.EQNYSDDVLANMISEPR.I + Oxidation (M)
18077 569 585 1038.9275 2075.8404 2075.8507 -4.97 0 16 0.028 1Score > 21 indicates identity
Score > 14 indicates homology
U K.EQNYSDDVLANMISEPR.I + Phospho (ST); Oxidation (M)
18078 569 585 692.9550 2075.8432 2075.8507 -3.59 0 22 0.0078 1Score > 21 indicates identity
Score > 14 indicates homology
U K.EQNYSDDVLANMISEPR.I + Phospho (ST); Oxidation (M)
15400 586 601 878.4264 1754.8382 1754.8396 -0.83 0 39 0.00021 1Score > 22 indicates identity
Score > 15 indicates homology
U R.ISYGNDALMPSLTETK.T + Oxidation (M)
15401 586 601 878.4270 1754.8394 1754.8396 -0.11 0 22 0.0092 1Score > 22 indicates identity
Score > 14 indicates homology
U R.ISYGNDALMPSLTETK.T + Oxidation (M)
15403 586 601 878.4278 1754.8411 1754.8396 0.82 0 84 1.4e-08 1Score > 21 indicates identity
Score > 18 indicates homology
U R.ISYGNDALMPSLTETK.T + Oxidation (M)
15404 586 601 878.4278 1754.8411 1754.8396 0.82 0 123 3.2e-12 1Score > 21 indicates identity
Score > 20 indicates homology
U R.ISYGNDALMPSLTETK.T + Oxidation (M)
12774 649 662 707.9045 1413.7945 1413.7940 0.37 0 66 6.8e-07 1Score > 16 indicates identity U R.GLTTRPGSGLTNIK.T
12775 649 662 707.9046 1413.7947 1413.7940 0.51 0 32 0.0016 1Score > 16 indicates identity U R.GLTTRPGSGLTNIK.T
20114 649 670 777.4240 2329.2503 2329.2489 0.61 1 44 8.6e-05 1Score > 15 indicates identity U R.GLTTRPGSGLTNIKTEEISEVK.M
7138 663 670 467.7403 933.4660 933.4655 0.61 0 10 0.8 2Score > 22 indicates identity U K.TEEISEVK.M
7139 663 670 467.7404 933.4663 933.4655 0.87 0 49 0.0001 1Score > 22 indicates identity U K.TEEISEVK.M
7140 663 670 467.7410 933.4674 933.4655 2.08 0 12 0.078 1Score > 21 indicates identity
Score > 14 indicates homology
U K.TEEISEVK.M
14994 663 676 567.2664 1698.7773 1698.7770 0.17 1 11 0.095 1Score > 22 indicates identity
Score > 13 indicates homology
U K.TEEISEVKMDAEFR.H + Oxidation (M)
14995 663 676 850.3960 1698.7775 1698.7770 0.28 1 81 5.4e-08 1Score > 22 indicates identity
Score > 20 indicates homology
U K.TEEISEVKMDAEFR.H + Oxidation (M)
18318 671 687 421.1887 2100.9074 2100.9072 0.088 1 15 0.039 1Score > 22 indicates identity
Score > 13 indicates homology
U K.MDAEFRHDSGYEVHHQK.L + Oxidation (M)
18320 671 687 701.3103 2100.9092 2100.9072 0.96 1 33 0.00073 1Score > 22 indicates identity
Score > 15 indicates homology
U K.MDAEFRHDSGYEVHHQK.L + Oxidation (M)
11846 677 687 668.8048 1335.5951 1335.5956 -0.38 0 44 7.7e-05 1Score > 21 indicates identity
Score > 15 indicates homology
U R.HDSGYEVHHQK.L
11847 677 687 446.2057 1335.5953 1335.5956 -0.23 0 38 0.00029 1Score > 21 indicates identity
Score > 15 indicates homology
U R.HDSGYEVHHQK.L
21850 677 699 529.4576 2642.2518 2642.2514 0.15 1 6 0.27 1Score > 22 indicates identity
Score > 13 indicates homology
U R.HDSGYEVHHQKLVFFAEDVGSNK.G
21851 677 699 661.5706 2642.2534 2642.2514 0.75 1 23 0.0077 1Score > 21 indicates identity
Score > 14 indicates homology
U R.HDSGYEVHHQKLVFFAEDVGSNK.G
11739 688 699 663.3413 1324.6681 1324.6663 1.37 0 13 0.06 1Score > 21 indicates identity
Score > 13 indicates homology
U K.LVFFAEDVGSNK.G
11740 688 699 663.3414 1324.6682 1324.6663 1.45 0 57 5e-06 1Score > 21 indicates identity
Score > 16 indicates homology
U K.LVFFAEDVGSNK.G
11741 688 699 663.3415 1324.6685 1324.6663 1.64 0 50 2.2e-05 1Score > 21 indicates identity
Score > 16 indicates homology
U K.LVFFAEDVGSNK.G
21075 726 747 493.8542 2464.2345 2464.2346 -0.075 1 22 0.0085 1Score > 21 indicates identity
Score > 14 indicates homology
U K.KQYTSIHHGVVEVDAAVTPEER.H
21076 726 747 822.4190 2464.2353 2464.2346 0.25 1 59 2.8e-06 1Score > 21 indicates identity
Score > 16 indicates homology
U K.KQYTSIHHGVVEVDAAVTPEER.H
20153 727 747 779.7210 2336.1413 2336.1397 0.69 0 38 0.00028 1Score > 22 indicates identity
Score > 15 indicates homology
U K.QYTSIHHGVVEVDAAVTPEER.H
20739 727 747 806.3779 2416.1120 2416.1060 2.47 0 33 0.00078 1Score > 22 indicates identity
Score > 15 indicates homology
U K.QYTSIHHGVVEVDAAVTPEER.H + Phospho (ST)
22356 727 751 561.2895 2801.4111 2801.4097 0.52 1 13 0.22 1Score > 19 indicates identity U K.QYTSIHHGVVEVDAAVTPEERHLSK.M
22357 727 751 561.2895 2801.4111 2801.4097 0.52 1 15 0.14 1Score > 19 indicates identity U K.QYTSIHHGVVEVDAAVTPEERHLSK.M
16900 748 763 651.9757 1952.9053 1952.9050 0.15 1 24 0.017 1Score > 22 indicates identity
Score > 19 indicates homology
U R.HLSKMQQNGYENPTYK.F + Oxidation (M)
20813 752 770 1215.0126 2428.0107 2428.0100 0.29 1 92 2.6e-09 1Score > 21 indicates identity
Score > 18 indicates homology
U K.MQQNGYENPTYKFFEQMQN.- + 2 Oxidation (M)
20814 752 770 810.3448 2428.0127 2428.0100 1.12 1 29 0.002 1Score > 21 indicates identity
Score > 14 indicates homology
U K.MQQNGYENPTYKFFEQMQN.- + 2 Oxidation (M)
7386 764 770 480.1999 958.3852 958.3855 -0.27 0 29 0.0058 1Score > 19 indicates identity U K.FFEQMQN.- + Oxidation (M)

Error distribution


ID   A4_HUMAN                Reviewed;         770 AA.
AC   P05067; B2R5V1; B4DII8; D3DSD1; D3DSD2; D3DSD3; P09000; P78438;
AC   Q13764; Q13778; Q13793; Q16011; Q16014; Q16019; Q16020; Q6GSC0;
AC   Q8WZ99; Q9BT38; Q9UC33; Q9UCA9; Q9UCB6; Q9UCC8; Q9UCD1; Q9UQ58;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 3.
DT   09-JUL-2014, entry version 229.
DE   RecName: Full=Amyloid beta A4 protein;
DE   AltName: Full=ABPP;
DE   AltName: Full=APPI;
DE            Short=APP;
DE   AltName: Full=Alzheimer disease amyloid protein;
DE   AltName: Full=Cerebral vascular amyloid peptide;
DE            Short=CVAP;
DE   AltName: Full=PreA4;
DE   AltName: Full=Protease nexin-II;
DE            Short=PN-II;
DE   Contains:
DE     RecName: Full=N-APP;
DE   Contains:
DE     RecName: Full=Soluble APP-alpha;
DE              Short=S-APP-alpha;
DE   Contains:
DE     RecName: Full=Soluble APP-beta;
DE              Short=S-APP-beta;
DE   Contains:
DE     RecName: Full=C99;
DE   Contains:
DE     RecName: Full=Beta-amyloid protein 42;
DE     AltName: Full=Beta-APP42;
DE   Contains:
DE     RecName: Full=Beta-amyloid protein 40;
DE     AltName: Full=Beta-APP40;
DE   Contains:
DE     RecName: Full=C83;
DE   Contains:
DE     RecName: Full=P3(42);
DE   Contains:
DE     RecName: Full=P3(40);
DE   Contains:
DE     RecName: Full=C80;
DE   Contains:
DE     RecName: Full=Gamma-secretase C-terminal fragment 59;
DE     AltName: Full=Amyloid intracellular domain 59;
DE              Short=AICD-59;
DE              Short=AID(59);
DE     AltName: Full=Gamma-CTF(59);
DE   Contains:
DE     RecName: Full=Gamma-secretase C-terminal fragment 57;
DE     AltName: Full=Amyloid intracellular domain 57;
DE              Short=AICD-57;
DE              Short=AID(57);
DE     AltName: Full=Gamma-CTF(57);
DE   Contains:
DE     RecName: Full=Gamma-secretase C-terminal fragment 50;
DE     AltName: Full=Amyloid intracellular domain 50;
DE              Short=AICD-50;
DE              Short=AID(50);
DE     AltName: Full=Gamma-CTF(50);
DE   Contains:
DE     RecName: Full=C31;
DE   Flags: Precursor;
GN   Name=APP; Synonyms=A4, AD1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM APP695).
RC   TISSUE=Brain;
RX   PubMed=2881207; DOI=10.1038/325733a0;
RA   Kang J., Lemaire H.-G., Unterbeck A., Salbaum J.M., Masters C.L.,
RA   Grzeschik K.-H., Multhaup G., Beyreuther K., Mueller-Hill B.;
RT   "The precursor of Alzheimer's disease amyloid A4 protein resembles a
RT   cell-surface receptor.";
RL   Nature 325:733-736(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM APP751).
RC   TISSUE=Brain;
RX   PubMed=2893289; DOI=10.1038/331525a0;
RA   Ponte P., Gonzalez-Dewhitt P., Schilling J., Miller J., Hsu D.,
RA   Greenberg B., Davis K., Wallace W., Lieberburg I., Fuller F.,
RA   Cordell B.;
RT   "A new A4 amyloid mRNA contains a domain homologous to serine
RT   proteinase inhibitors.";
RL   Nature 331:525-527(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM APP695).
RX   PubMed=2783775; DOI=10.1093/nar/17.2.517;
RA   Lemaire H.-G., Salbaum J.M., Multhaup G., Kang J., Bayney R.M.,
RA   Unterbeck A., Beyreuther K., Mueller-Hill B.;
RT   "The PreA4(695) precursor protein of Alzheimer's disease A4 amyloid is
RT   encoded by 16 exons.";
RL   Nucleic Acids Res. 17:517-522(1989).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM APP770).
RX   PubMed=2110105; DOI=10.1016/0378-1119(90)90310-N;
RA   Yoshikai S., Sasaki H., Doh-ura K., Furuya H., Sakaki Y.;
RT   "Genomic organization of the human amyloid beta-protein precursor
RT   gene.";
RL   Gene 87:257-263(1990).
RN   [5]
RP   ERRATUM.
RX   PubMed=1908403; DOI=10.1016/0378-1119(91)90093-Q;
RA   Yoshikai S., Sasaki H., Doh-ura K., Furuya H., Sakaki Y.;
RL   Gene 102:291-292(1991).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM L-APP733).
RC   TISSUE=Leukocyte;
RX   PubMed=1587857;
RA   Koenig G., Moenning U., Czech C., Prior R., Banati R.,
RA   Schreiter-Gasser U., Bauer J., Masters C.L., Beyreuther K.;
RT   "Identification and differential expression of a novel alternative
RT   splice isoform of the beta A4 amyloid precursor protein (APP) mRNA in
RT   leukocytes and brain microglial cells.";
RL   J. Biol. Chem. 267:10804-10809(1992).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM APP770).
RX   PubMed=9108164; DOI=10.1093/nar/25.9.1802;
RA   Hattori M., Tsukahara F., Furuhata Y., Tanahashi H., Hirose M.,
RA   Saito M., Tsukuni S., Sakaki Y.;
RT   "A novel method for making nested deletions and its application for
RT   sequencing of a 300 kb region of human APP locus.";
RL   Nucleic Acids Res. 25:1802-1808(1997).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM APP639), AND TISSUE SPECIFICITY.
RC   TISSUE=Brain;
RX   PubMed=12859342; DOI=10.1046/j.1460-9568.2003.02731.x;
RA   Tang K., Wang C., Shen C., Sheng S., Ravid R., Jing N.;
RT   "Identification of a novel alternative splicing isoform of human
RT   amyloid precursor protein gene, APP639.";
RL   Eur. J. Neurosci. 18:102-108(2003).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS APP770 AND 11).
RC   TISSUE=Cerebellum, and Hippocampus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LYS-501.
RG   NIEHS SNPs program;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10830953; DOI=10.1038/35012518;
RA   Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T.,
RA   Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y.,
RA   Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K.,
RA   Polley A., Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D.,
RA   Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W.,
RA   Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S.,
RA   Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E.,
RA   Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P.,
RA   Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H.,
RA   Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E.,
RA   Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F.,
RA   Lehrach H., Reinhardt R., Yaspo M.-L.;
RT   "The DNA sequence of human chromosome 21.";
RL   Nature 405:311-319(2000).
RN   [12]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [13]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS APP305 AND APP751).
RC   TISSUE=Eye, and Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [14]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-10.
RC   TISSUE=Liver;
RX   PubMed=3140222; DOI=10.1093/nar/16.19.9351;
RA   Schon E.A., Mita S., Sadlock J., Herbert J.;
RT   "A cDNA specifying the human amyloid beta precursor protein (ABPP)
RT   encodes a 95-kDa polypeptide.";
RL   Nucleic Acids Res. 16:9351-9351(1988).
RN   [15]
RP   ERRATUM, AND SEQUENCE REVISION.
RA   Schon E.A., Mita S., Sadlock J., Herbert J.;
RL   Nucleic Acids Res. 16:11402-11402(1988).
RN   [16]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75.
RX   PubMed=2538123; DOI=10.1016/0006-291X(89)92437-6;
RA   La Fauci G., Lahiri D.K., Salton S.R., Robakis N.K.;
RT   "Characterization of the 5'-end region and the first two exons of the
RT   beta-protein precursor gene.";
RL   Biochem. Biophys. Res. Commun. 159:297-304(1989).
RN   [17]
RP   PROTEIN SEQUENCE OF 18-50.
RC   TISSUE=Fibroblast;
RX   PubMed=3597385;
RA   van Nostrand W.E., Cunningham D.D.;
RT   "Purification of protease nexin II from human fibroblasts.";
RL   J. Biol. Chem. 262:8508-8514(1987).
RN   [18]
RP   PROTEIN SEQUENCE OF 18-40.
RC   TISSUE=Platelet;
RX   PubMed=12665801; DOI=10.1038/nbt810;
RA   Gevaert K., Goethals M., Martens L., Van Damme J., Staes A.,
RA   Thomas G.R., Vandekerckhove J.;
RT   "Exploring proteomes and analyzing protein processing by mass
RT   spectrometric identification of sorted N-terminal peptides.";
RL   Nat. Biotechnol. 21:566-569(2003).
RN   [19]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 286-366.
RX   PubMed=2893290; DOI=10.1038/331528a0;
RA   Tanzi R.E., McClatchey A.I., Lamperti E.D., Villa-Komaroff L.,
RA   Gusella J.F., Neve R.L.;
RT   "Protease inhibitor domain encoded by an amyloid protein precursor
RT   mRNA associated with Alzheimer's disease.";
RL   Nature 331:528-530(1988).
RN   [20]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 287-367.
RX   PubMed=2893291; DOI=10.1038/331530a0;
RA   Kitaguchi N., Takahashi Y., Tokushima Y., Shiojiri S., Ito H.;
RT   "Novel precursor of Alzheimer's disease amyloid protein shows protease
RT   inhibitory activity.";
RL   Nature 331:530-532(1988).
RN   [21]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 507-770.
RC   TISSUE=Brain cortex;
RX   PubMed=2893379; DOI=10.1073/pnas.85.3.929;
RA   Zain S.B., Salim M., Chou W.G., Sajdel-Sulkowska E.M., Majocha R.E.,
RA   Marotta C.A.;
RT   "Molecular cloning of amyloid cDNA derived from mRNA of the Alzheimer
RT   disease brain: coding and noncoding regions of the fetal precursor
RT   mRNA are expressed in the cortex.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:929-933(1988).
RN   [22]
RP   PROTEIN SEQUENCE OF 523-555, AND DOMAIN COLLAGEN-BINDING.
RX   PubMed=8576160; DOI=10.1074/jbc.271.3.1613;
RA   Beher D., Hesse L., Masters C.L., Multhaup G.;
RT   "Regulation of amyloid protein precursor (APP) binding to collagen and
RT   mapping of the binding sites on APP and collagen type I.";
RL   J. Biol. Chem. 271:1613-1620(1996).
RN   [23]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 655-737, AND VARIANTS AD1 GLY-717;
RP   ILE-717 AND PHE-717.
RX   PubMed=8476439; DOI=10.1006/bbrc.1993.1386;
RA   Denman R.B., Rosenzcwaig R., Miller D.L.;
RT   "A system for studying the effect(s) of familial Alzheimer disease
RT   mutations on the processing of the beta-amyloid peptide precursor.";
RL   Biochem. Biophys. Res. Commun. 192:96-103(1993).
RN   [24]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 656-737.
RX   PubMed=2675837; DOI=10.1016/0006-291X(89)91112-1;
RA   Johnstone E.M., Chaney M.O., Moore R.E., Ward K.E., Norris F.H.,
RA   Little S.P.;
RT   "Alzheimer's disease amyloid peptide is encoded by two exons and shows
RT   similarity to soybean trypsin inhibitor.";
RL   Biochem. Biophys. Res. Commun. 163:1248-1255(1989).
RN   [25]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 672-723, AND VARIANT AD1 ASN-678.
RX   PubMed=15201367; DOI=10.1136/jnnp.2003.010611;
RA   Wakutani Y., Watanabe K., Adachi Y., Wada-Isoe K., Urakami K.,
RA   Ninomiya H., Saido T.C., Hashimoto T., Iwatsubo T., Nakashima K.;
RT   "Novel amyloid precursor protein gene missense mutation (D678N) in
RT   probable familial Alzheimer's disease.";
RL   J. Neurol. Neurosurg. Psych. 75:1039-1042(2004).
RN   [26]
RP   PROTEIN SEQUENCE OF 672-713.
RC   TISSUE=Blood vessel;
RX   PubMed=8248178; DOI=10.1073/pnas.90.22.10836;
RA   Roher A.E., Lowenson J.D., Clarke S., Woods A.S., Cotter R.J.,
RA   Gowing E., Ball M.J.;
RT   "Beta-amyloid-(1-42) is a major component of cerebrovascular amyloid
RT   deposits: implications for the pathology of Alzheimer disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:10836-10840(1993).
RN   [27]
RP   PROTEIN SEQUENCE OF 672-704, AND TISSUE SPECIFICITY.
RX   PubMed=1406936; DOI=10.1038/359325a0;
RA   Seubert P., Vigo-Pelfrey C., Esch F., Lee M., Dovey H., Davis D.,
RA   Sinha S., Schlossmacher M., Whaley J., Swindlehurst C.;
RT   "Isolation and quantification of soluble Alzheimer's beta-peptide from
RT   biological fluids.";
RL   Nature 359:325-327(1992).
RN   [28]
RP   PROTEIN SEQUENCE OF 672-701 AND 707-713.
RX   PubMed=8109908; DOI=10.1002/ana.410350223;
RA   Wisniewski T., Lalowski M., Levy E., Marques M.R.F., Frangione B.;
RT   "The amino acid sequence of neuritic plaque amyloid from a familial
RT   Alzheimer's disease patient.";
RL   Ann. Neurol. 35:245-246(1994).
RN   [29]
RP   PROTEIN SEQUENCE OF 672-701.
RC   TISSUE=Cerebrospinal fluid;
RX   PubMed=8229004; DOI=10.1111/j.1471-4159.1993.tb09841.x;
RA   Vigo-Pelfrey C., Lee D., Keim P., Lieberburg I., Schenk D.B.;
RT   "Characterization of beta-amyloid peptide from human cerebrospinal
RT   fluid.";
RL   J. Neurochem. 61:1965-1968(1993).
RN   [30]
RP   PROTEIN SEQUENCE OF 672-681.
RC   TISSUE=Brain cortex;
RX   PubMed=3312495; DOI=10.1111/j.1471-4159.1987.tb01005.x;
RA   Pardridge W.M., Vinters H.V., Yang J., Eisenberg J., Choi T.B.,
RA   Tourtellotte W.W., Huebner V., Shively J.E.;
RT   "Amyloid angiopathy of Alzheimer's disease: amino acid composition and
RT   partial sequence of a 4,200-dalton peptide isolated from cortical
RT   microvessels.";
RL   J. Neurochem. 49:1394-1401(1987).
RN   [31]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 674-770.
RC   TISSUE=Brain;
RX   PubMed=3810169; DOI=10.1126/science.3810169;
RA   Goldgaber D., Lerman M.I., McBride O.W., Saffiotti U., Gajdusek D.C.;
RT   "Characterization and chromosomal localization of a cDNA encoding
RT   brain amyloid of Alzheimer's disease.";
RL   Science 235:877-880(1987).
RN   [32]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 674-703.
RC   TISSUE=Fetal brain;
RX   PubMed=2949367; DOI=10.1126/science.2949367;
RA   Tanzi R.E., Gusella J.F., Watkins P.C., Bruns G.A.,
RA   St George-Hyslop P.H., Van Keuren M.L., Patterson D., Pagan S.,
RA   Kurnit D.M., Neve R.L.;
RT   "Amyloid beta protein gene: cDNA, mRNA distribution, and genetic
RT   linkage near the Alzheimer locus.";
RL   Science 235:880-884(1987).
RN   [33]
RP   PROTEIN SEQUENCE OF 609-713, AND GLYCOSYLATION AT SER-614; SER-623;
RP   SER-628; SER-679 AND SER-697.
RC   TISSUE=Cerebrospinal fluid;
RX   PubMed=22576872; DOI=10.1002/jms.2987;
RA   Brinkmalm G., Portelius E., Ohrfelt A., Mattsson N., Persson R.,
RA   Gustavsson M.K., Vite C.H., Gobom J., Mansson J.E., Nilsson J.,
RA   Halim A., Larson G., Ruetschi U., Zetterberg H., Blennow K.,
RA   Brinkmalm A.;
RT   "An online nano-LC-ESI-FTICR-MS method for comprehensive
RT   characterization of endogenous fragments from amyloid beta and amyloid
RT   precursor protein in human and cat cerebrospinal fluid.";
RL   J. Mass Spectrom. 47:591-603(2012).
RN   [34]
RP   PROTEIN SEQUENCE OF 691-698, AND CLEAVAGE BY THETA-SECRETASE.
RX   PubMed=16816112; DOI=10.1096/fj.05-5632com;
RA   Sun X., He G., Song W.;
RT   "BACE2, as a novel APP theta-secretase, is not responsible for the
RT   pathogenesis of Alzheimer's disease in Down syndrome.";
RL   FASEB J. 20:1369-1376(2006).
RN   [35]
RP   PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM APP751).
RC   TISSUE=Brain;
RX   PubMed=2569763; DOI=10.1126/science.2569763;
RA   de Sauvage F., Octave J.-N.;
RT   "A novel mRNA of the A4 amyloid precursor gene coding for a possibly
RT   secreted protein.";
RL   Science 245:651-653(1989).
RN   [36]
RP   PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM APP695).
RC   TISSUE=Brain;
RX   PubMed=3035574; DOI=10.1073/pnas.84.12.4190;
RA   Robakis N.K., Ramakrishna N., Wolfe G., Wisniewski H.M.;
RT   "Molecular cloning and characterization of a cDNA encoding the
RT   cerebrovascular and the neuritic plaque amyloid peptides.";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:4190-4194(1987).
RN   [37]
RP   CHARACTERIZATION OF L-APP733, AND MUTAGENESIS OF SER-656.
RX   PubMed=7737970; DOI=10.1074/jbc.270.18.10388;
RA   Pangalos M.N., Efthimiopoulos S., Shioi J., Robakis N.K.;
RT   "The chondroitin sulfate attachment site of appican is formed by
RT   splicing out exon 15 of the amyloid precursor gene.";
RL   J. Biol. Chem. 270:10388-10391(1995).
RN   [38]
RP   FUNCTION OF BETA-AMYLOID PEPTIDE AS LIPID PEROXIDATION INHIBITOR, AND
RP   MUTAGENESIS OF MET-706.
RX   PubMed=9168929; DOI=10.1006/bbrc.1997.6547;
RA   Walter M.F., Mason P.E., Mason R.P.;
RT   "Alzheimer's disease amyloid beta peptide 25-35 inhibits lipid
RT   peroxidation as a result of its membrane interactions.";
RL   Biochem. Biophys. Res. Commun. 233:760-764(1997).
RN   [39]
RP   REVIEW ON FUNCTION OF BETA-AMYLOID AS ANTIOXIDANT.
RX   PubMed=11775062; DOI=10.1023/A:1012629603390;
RA   Kontush A.;
RT   "Alzheimer's amyloid-beta as a preventive antioxidant for brain
RT   lipoproteins.";
RL   Cell. Mol. Neurobiol. 21:299-315(2001).
RN   [40]
RP   IDENTITY OF APP WITH NEXIN-II.
RX   PubMed=2506449; DOI=10.1038/341144a0;
RA   Oltersdorf T., Fritz L.C., Schenk D.B., Lieberburg I.,
RA   Johnson-Wood K.L., Beattie E.C., Ward P.J., Blacher R.W., Dovey H.F.,
RA   Sinha S.;
RT   "The secreted form of the Alzheimer's amyloid precursor protein with
RT   the Kunitz domain is protease nexin-II.";
RL   Nature 341:144-147(1989).
RN   [41]
RP   PROTEASE-SPECIFICITY OF INHIBITOR DOMAIN.
RX   PubMed=1969731; DOI=10.1016/0006-291X(90)92084-D;
RA   Kido H., Fukutomi A., Schilling J., Wang Y., Cordell B., Katunuma N.;
RT   "Protease-specificity of Kunitz inhibitor domain of Alzheimer's
RT   disease amyloid protein precursor.";
RL   Biochem. Biophys. Res. Commun. 167:716-721(1990).
RN   [42]
RP   EXTRACELLULAR ZINC-BINDING DOMAIN.
RX   PubMed=8344894;
RA   Bush A.I., Multhaup G., Moir R.D., Williamson T.G., Small D.H.,
RA   Rumble B., Pollwein P., Beyreuther K., Masters C.L.;
RT   "A novel zinc(II) binding site modulates the function of the beta A4
RT   amyloid protein precursor of Alzheimer's disease.";
RL   J. Biol. Chem. 268:16109-16112(1993).
RN   [43]
RP   INTERACTION WITH G(O).
RX   PubMed=8446172; DOI=10.1038/362075a0;
RA   Nishimoto I., Okamoto T., Matsuura Y., Takahashi S., Okamoto T.,
RA   Murayama Y., Ogata E.;
RT   "Alzheimer amyloid protein precursor complexes with brain GTP-binding
RT   protein G(o).";
RL   Nature 362:75-79(1993).
RN   [44]
RP   EXTRACELLULAR COPPER-BINDING DOMAIN, AND MUTAGENESIS OF HIS-137;
RP   MET-141; CYS-144; HIS-147 AND HIS-151.
RX   PubMed=7913895; DOI=10.1016/0014-5793(94)00658-X;
RA   Hesse L., Beher D., Masters C.L., Multhaup G.;
RT   "The beta A4 amyloid precursor protein binding to copper.";
RL   FEBS Lett. 349:109-116(1994).
RN   [45]
RP   N-TERMINAL HEPARIN-BINDING DOMAIN, AND MUTAGENESIS OF 99-LYS--ARG-102.
RX   PubMed=8158260;
RA   Small D.H., Nurcombe V., Reed G., Clarris H., Moir R., Beyreuther K.,
RA   Masters C.L.;
RT   "A heparin-binding domain in the amyloid protein precursor of
RT   Alzheimer's disease is involved in the regulation of neurite
RT   outgrowth.";
RL   J. Neurosci. 14:2117-2127(1994).
RN   [46]
RP   MUTAGENESIS OF VAL-717.
RX   PubMed=8886002; DOI=10.1006/bbrc.1996.1577;
RA   Maruyama K., Tomita T., Shinozaki K., Kume H., Asada H., Saido T.C.,
RA   Ishiura S., Iwatsubo T., Obata K.;
RT   "Familial Alzheimer's disease-linked mutations at Val717 of amyloid
RT   precursor protein are specific for the increased secretion of A beta
RT   42(43).";
RL   Biochem. Biophys. Res. Commun. 227:730-735(1996).
RN   [47]
RP   INTERACTION WITH APP-BP1.
RX   PubMed=8626687; DOI=10.1074/jbc.271.19.11339;
RA   Chow N., Korenberg J.R., Chen X.-N., Neve R.L.;
RT   "APP-BP1, a novel protein that binds to the carboxyl-terminal region
RT   of the amyloid precursor protein.";
RL   J. Biol. Chem. 271:11339-11346(1996).
RN   [48]
RP   INTERACTION WITH APBA1 AND APBB1, AND MUTAGENESIS OF TYR-728; TYR-757;
RP   ASN-759 AND TYR-762.
RX   PubMed=8887653;
RA   Borg J.-P., Ooi J., Levy E., Margolis B.;
RT   "The phosphotyrosine interaction domains of X11 and FE65 bind to
RT   distinct sites on the YENPTY motif of amyloid precursor protein.";
RL   Mol. Cell. Biol. 16:6229-6241(1996).
RN   [49]
RP   INTERACTION WITH APBB2.
RX   PubMed=8855266; DOI=10.1073/pnas.93.20.10832;
RA   Guenette S.Y., Chen J., Jondro P.D., Tanzi R.E.;
RT   "Association of a novel human FE65-like protein with the cytoplasmic
RT   domain of the beta-amyloid precursor protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:10832-10837(1996).
RN   [50]
RP   HEPARIN-BINDING DOMAINS.
RX   PubMed=9357988; DOI=10.1016/S0014-5793(97)01146-0;
RA   Mok S.S., Sberna G., Heffernan D., Cappai R., Galatis D.,
RA   Clarris H.J., Sawyer W.H., Beyreuther K., Masters C.L., Small D.H.;
RT   "Expression and analysis of heparin-binding regions of the amyloid
RT   precursor protein of Alzheimer's disease.";
RL   FEBS Lett. 415:303-307(1997).
RN   [51]
RP   INTERACTION OF BETA-AMYLOID PEPTIDE WITH HADH2.
RC   TISSUE=Brain;
RX   PubMed=9338779; DOI=10.1038/39522;
RA   Yan S.D., Fu J., Soto C., Chen X., Zhu H., Al-Mohanna F.,
RA   Collinson K., Zhu A., Stern E., Saido T., Tohyama M., Ogawa S.,
RA   Roher A., Stern D.;
RT   "An intracellular protein that binds amyloid-beta peptide and mediates
RT   neurotoxicity in Alzheimer's disease.";
RL   Nature 389:689-695(1997).
RN   [52]
RP   INTERACTION WITH APPBP2, AND MUTAGENESIS OF TYR-728.
RX   PubMed=9843960; DOI=10.1073/pnas.95.25.14745;
RA   Zheng P., Eastman J., Vande Pol S., Pimplikar S.W.;
RT   "PAT1, a microtubule-interacting protein, recognizes the basolateral
RT   sorting signal of amyloid precursor protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:14745-14750(1998).
RN   [53]
RP   BETA-AMYLOID ZINC-BINDING, AND MUTAGENESIS OF ARG-676; TYR-681 AND
RP   HIS-684.
RX   PubMed=10413512; DOI=10.1021/bi990205o;
RA   Liu S.T., Howlett G., Barrow C.J.;
RT   "Histidine-13 is a crucial residue in the zinc ion-induced aggregation
RT   of the A beta peptide of Alzheimer's disease.";
RL   Biochemistry 38:9373-9378(1999).
RN   [54]
RP   IMPORTANCE OF MET-706 IN FREE RADICAL OXIDATIVE STRESS, AND
RP   MUTAGENESIS OF MET-706.
RX   PubMed=10535332; DOI=10.1016/S0361-9230(99)00093-3;
RA   Varadarajan S., Yatin S., Kanski J., Jahanshahi F., Butterfield D.A.;
RT   "Methionine residue 35 is important in amyloid beta-peptide-associated
RT   free radical oxidative stress.";
RL   Brain Res. Bull. 50:133-141(1999).
RN   [55]
RP   INTERACTION WITH APBA2.
RX   PubMed=9890987; DOI=10.1074/jbc.274.4.2243;
RA   Tomita S., Ozaki T., Taru H., Oguchi S., Takeda S., Yagi Y.,
RA   Sakiyama S., Kirino Y., Suzuki T.;
RT   "Interaction of a neuron-specific protein containing PDZ domains with
RT   Alzheimer's amyloid precursor protein.";
RL   J. Biol. Chem. 274:2243-2254(1999).
RN   [56]
RP   ENDOCYTOSIS SIGNAL, AND MUTAGENESIS OF TYR-728; GLY-756; TYR-757;
RP   ASN-759; PRO-760 AND TYR-762.
RX   PubMed=10383380; DOI=10.1074/jbc.274.27.18851;
RA   Perez R.G., Soriano S., Hayes J.D., Ostaszewski B., Xia W.,
RA   Selkoe D.J., Chen X., Stokin G.B., Koo E.H.;
RT   "Mutagenesis identifies new signals for beta-amyloid precursor protein
RT   endocytosis, turnover, and the generation of secreted fragments,
RT   including Abeta42.";
RL   J. Biol. Chem. 274:18851-18856(1999).
RN   [57]
RP   IMPORTANCE OF CYS-144 IN COPPER REDUCTION, AND MUTAGENESIS OF CYS-144
RP   AND 147-HIS--HIS-149.
RX   PubMed=10461923; DOI=10.1046/j.1471-4159.1999.0731288.x;
RA   Ruiz F.H., Gonzalez M., Bodini M., Opazo C., Inestrosa N.C.;
RT   "Cysteine 144 is a key residue in the copper reduction by the beta-
RT   amyloid precursor protein.";
RL   J. Neurochem. 73:1288-1292(1999).
RN   [58]
RP   INTERACTION OF BETA-AMYLOID WITH APOE.
RX   PubMed=10816430; DOI=10.1042/0264-6021:3480359;
RA   Tokuda T., Calero M., Matsubara E., Vidal R., Kumar A., Permanne B.,
RA   Zlokovic B., Smith J.D., Ladu M.J., Rostagno A., Frangione B.,
RA   Ghiso J.;
RT   "Lipidation of apolipoprotein E influences its isoform-specific
RT   interaction with Alzheimer's amyloid beta peptides.";
RL   Biochem. J. 348:359-365(2000).
RN   [59]
RP   INTERACTION OF BETA-APP42 WITH CHRNA7.
RX   PubMed=10681545; DOI=10.1074/jbc.275.8.5626;
RA   Wang H.-Y., Lee D.H.S., D'Andrea M.R., Peterson P.A., Shank R.P.,
RA   Reitz A.B.;
RT   "Beta-amyloid(1-42) binds to alpha7 nicotinic acetylcholine receptor
RT   with high affinity. Implications for Alzheimer's disease pathology.";
RL   J. Biol. Chem. 275:5626-5632(2000).
RN   [60]
RP   IDENTIFICATION OF GAMMA-CTFS BY MASS SPECTROMETRY, AND MUTAGENESIS OF
RP   ASP-739.
RX   PubMed=12214090;
RA   Passer B., Pellegrini L., Russo C., Siegel R.M., Lenardo M.J.,
RA   Schettini G., Bachmann M., Tabaton M., D'Adamio L.;
RT   "Generation of an apoptotic intracellular peptide by gamma-secretase
RT   cleavage of Alzheimer's amyloid beta protein precursor.";
RL   J. Alzheimers Dis. 2:289-301(2000).
RN   [61]
RP   INTERACTION WITH FPRL1.
RX   PubMed=11689470; DOI=10.1096/fj.01-0251com;
RA   Yazawa H., Yu Z.-X., Takeda K., Le Y., Gong W., Ferrans V.J.,
RA   Oppenheim J.J., Li C.C.H., Wang J.M.;
RT   "Beta amyloid peptide (Abeta42) is internalized via the G-protein-
RT   coupled receptor FPRL1 and forms fibrillar aggregates in
RT   macrophages.";
RL   FASEB J. 15:2454-2462(2001).
RN   [62]
RP   INTERACTION WITH BBP.
RX   PubMed=11278849; DOI=10.1074/jbc.M011161200;
RA   Kajkowski E.M., Lo C.F., Ning X., Walker S., Sofia H.J., Wang W.,
RA   Edris W., Chanda P., Wagner E., Vile S., Ryan K., McHendry-Rinde B.,
RA   Smith S.C., Wood A., Rhodes K.J., Kennedy J.D., Bard J.,
RA   Jacobsen J.S., Ozenberger B.A.;
RT   "Beta-amyloid peptide-induced apoptosis regulated by a novel protein
RT   containing a G protein activation module.";
RL   J. Biol. Chem. 276:18748-18756(2001).
RN   [63]
RP   BETA-AMYLOID COPPER AND ZINC-BINDING.
RX   PubMed=11274207; DOI=10.1074/jbc.M100175200;
RA   Curtain C.C., Ali F., Volitakis I., Cherny R.A., Norton R.S.,
RA   Beyreuther K., Barrow C.J., Masters C.L., Bush A.I., Barnham K.J.;
RT   "Alzheimer's disease amyloid-beta binds copper and zinc to generate an
RT   allosterically ordered structure containing superoxide dismutase-like
RT   subunits.";
RL   J. Biol. Chem. 276:20466-20473(2001).
RN   [64]
RP   SUBUNIT.
RX   PubMed=11438549; DOI=10.1074/jbc.M105410200;
RA   Scheuermann S., Hambsch B., Hesse L., Stumm J., Schmidt C., Beher D.,
RA   Bayer T.A., Beyreuther K., Multhaup G.;
RT   "Homodimerization of amyloid precursor protein and its implication in
RT   the amyloidogenic pathway of Alzheimer's disease.";
RL   J. Biol. Chem. 276:33923-33929(2001).
RN   [65]
RP   INTERACTION WITH APBB1, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=11544248; DOI=10.1074/jbc.C100447200;
RA   Kimberly W.T., Zheng J.B., Guenette S.Y., Selkoe D.J.;
RT   "The intracellular domain of the beta-amyloid precursor protein is
RT   stabilized by Fe65 and translocates to the nucleus in a notch-like
RT   manner.";
RL   J. Biol. Chem. 276:40288-40292(2001).
RN   [66]
RP   INTERACTION WITH FBLN1.
RX   PubMed=11238726; DOI=10.1046/j.1471-4159.2001.00144.x;
RA   Ohsawa I., Takamura C., Kohsaka S.;
RT   "Fibulin-1 binds the amino-terminal head of beta-amyloid precursor
RT   protein and modulates its physiological function.";
RL   J. Neurochem. 76:1411-1420(2001).
RN   [67]
RP   INTERACTION WITH MAPT, AND FUNCTION.
RX   PubMed=11943163; DOI=10.1016/S0014-5793(02)02376-1;
RA   Rank K.B., Pauley A.M., Bhattacharya K., Wang Z., Evans D.B.,
RA   Fleck T.J., Johnston J.A., Sharma S.K.;
RT   "Direct interaction of soluble human recombinant tau protein with
RT   Abeta 1-42 results in tau aggregation and hyperphosphorylation by tau
RT   protein kinase II.";
RL   FEBS Lett. 514:263-268(2002).
RN   [68]
RP   INTERACTION WITH MAPK8IP1, AND MUTAGENESIS OF TYR-757.
RX   PubMed=11724784; DOI=10.1074/jbc.M108357200;
RA   Scheinfeld M.H., Roncarati R., Vito P., Lopez P.A., Abdallah M.,
RA   D'Adamio L.;
RT   "Jun NH2-terminal kinase (JNK) interacting protein 1 (JIP1) binds the
RT   cytoplasmic domain of the Alzheimer's beta-amyloid precursor protein
RT   (APP).";
RL   J. Biol. Chem. 277:3767-3775(2002).
RN   [69]
RP   COPPER-MEDIATED LIPID PEROXIDATION, AND MUTAGENESIS OF HIS-147 AND
RP   HIS-151.
RX   PubMed=11784781;
RA   White A.R., Multhaup G., Galatis D., McKinstry W.J., Parker M.W.,
RA   Pipkorn R., Beyreuther K., Masters C.L., Cappai R.;
RT   "Contrasting species-dependent modulation of copper-mediated
RT   neurotoxicity by the Alzheimer's disease amyloid precursor protein.";
RL   J. Neurosci. 22:365-376(2002).
RN   [70]
RP   REVIEW ON ZINC-BINDING.
RX   PubMed=12032279; DOI=10.1073/pnas.122249699;
RA   Bush A.I., Tanzi R.E.;
RT   "The galvanization of beta-amyloid in Alzheimer's disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:7317-7319(2002).
RN   [71]
RP   SUBCELLULAR LOCATION, AND ASSOCIATION OF AMYLOID FIBRILS WITH GCP1.
RX   PubMed=15084524; DOI=10.1096/fj.03-1040fje;
RA   Watanabe N., Araki W., Chui D.H., Makifuchi T., Ihara Y., Tabira T.;
RT   "Glypican-1 as an Abeta binding HSPG in the human brain: its
RT   localization in DIG domains and possible roles in the pathogenesis of
RT   Alzheimer's disease.";
RL   FASEB J. 18:1013-1015(2004).
RN   [72]
RP   INTERACTION WITH ANKS1B.
RX   PubMed=15347684; DOI=10.1074/jbc.M405329200;
RA   Ghersi E., Noviello C., D'Adamio L.;
RT   "Amyloid-beta protein precursor (AbetaPP) intracellular domain-
RT   associated protein-1 proteins bind to AbetaPP and modulate its
RT   processing in an isoform-specific manner.";
RL   J. Biol. Chem. 279:49105-49112(2004).
RN   [73]
RP   PHOSPHORYLATION AT THR-743.
RX   PubMed=8131745;
RA   Suzuki T., Oishi M., Marshak D.R., Czernik A.J., Nairn A.C.,
RA   Greengard P.;
RT   "Cell cycle-dependent regulation of the phosphorylation and metabolism
RT   of the Alzheimer amyloid precursor protein.";
RL   EMBO J. 13:1114-1122(1994).
RN   [74]
RP   PHOSPHORYLATION AT SER-198 AND SER-206 BY CASEIN KINASES, AND
RP   MUTAGENESIS OF SER-198 AND SER-206.
RX   PubMed=8999878; DOI=10.1074/jbc.272.3.1896;
RA   Walter J., Capell A., Hung A.Y., Langen H., Schnoelzer M.,
RA   Thinakaran G., Sisodia S.S., Selkoe D.J., Haass C.;
RT   "Ectodomain phosphorylation of beta-amyloid precursor protein at two
RT   distinct cellular locations.";
RL   J. Biol. Chem. 272:1896-1903(1997).
RN   [75]
RP   COPPER-BINDING, AND DISULFIDE BOND FORMATION.
RX   PubMed=9585534; DOI=10.1021/bi980022m;
RA   Multhaup G., Ruppert T., Schlicksupp A., Hesse L., Bill E.,
RA   Pipkorn R., Masters C.L., Beyreuther K.;
RT   "Copper-binding amyloid precursor protein undergoes a site-specific
RT   fragmentation in the reduction of hydrogen peroxide.";
RL   Biochemistry 37:7224-7230(1998).
RN   [76]
RP   CLEAVAGE BY CASPASES, AND MUTAGENESIS OF ASP-739.
RX   PubMed=10319819; DOI=10.1016/S0092-8674(00)80748-5;
RA   Gervais F.G., Xu D., Robertson G.S., Vaillancourt J.P., Zhu Y.,
RA   Huang J., LeBlanc A., Smith D., Rigby M., Shearman M.S., Clarke E.E.,
RA   Zheng H., van der Ploeg L.H.T., Ruffolo S.C., Thornberry N.A.,
RA   Xanthoudakis S., Zamboni R.J., Roy S., Nicholson D.W.;
RT   "Involvement of caspases in proteolytic cleavage of Alzheimer's
RT   amyloid-beta precursor protein and amyloidogenic A beta peptide
RT   formation.";
RL   Cell 97:395-406(1999).
RN   [77]
RP   PHOSPHORYLATION, AND MUTAGENESIS OF THR-743.
RX   PubMed=10341243;
RA   Ando K., Oishi M., Takeda S., Iijima K., Isohara T., Nairn A.C.,
RA   Kirino Y., Greengard P., Suzuki T.;
RT   "Role of phosphorylation of Alzheimer's amyloid precursor protein
RT   during neuronal differentiation.";
RL   J. Neurosci. 19:4421-4427(1999).
RN   [78]
RP   CHARACTERIZATION OF CASEIN KINASE PHOSPHORYLATION, AND MUTAGENESIS OF
RP   SER-198 AND SER-206.
RX   PubMed=10806211; DOI=10.1074/jbc.M002850200;
RA   Walter J., Schindzielorz A., Hartung B., Haass C.;
RT   "Phosphorylation of the beta-amyloid precursor protein at the cell
RT   surface by ectocasein kinases 1 and 2.";
RL   J. Biol. Chem. 275:23523-23529(2000).
RN   [79]
RP   CLEAVAGE BY CASPASES, AND MUTAGENESIS OF ASP-739.
RX   PubMed=10742146; DOI=10.1038/74656;
RA   Lu D.C., Rabizadeh S., Chandra S., Shayya R.F., Ellerby L.M., Ye X.,
RA   Salvesen G.S., Koo E.H., Bredesen D.E.;
RT   "A second cytotoxic proteolytic peptide derived from amyloid beta-
RT   protein precursor.";
RL   Nat. Med. 6:397-404(2000).
RN   [80]
RP   PHOSPHORYLATION, INTERACTION WITH APBB1, AND MUTAGENESIS OF THR-743.
RX   PubMed=11517218; DOI=10.1074/jbc.M104059200;
RA   Ando K., Iijima K., Elliott J.I., Kirino Y., Suzuki T.;
RT   "Phosphorylation-dependent regulation of the interaction of amyloid
RT   precursor protein with Fe65 affects the production of beta-amyloid.";
RL   J. Biol. Chem. 276:40353-40361(2001).
RN   [81]
RP   PHOSPHORYLATION BY MAPK10, AND MUTAGENESIS OF THR-743.
RX   PubMed=11146006; DOI=10.1046/j.1471-4159.2001.00102.x;
RA   Standen C.L., Brownlees J., Grierson A.J., Kesavapany S., Lau K.-F.,
RA   McLoughlin D.M., Miller C.C.J.;
RT   "Phosphorylation of thr(668) in the cytoplasmic domain of the
RT   Alzheimer's disease amyloid precursor protein by stress-activated
RT   protein kinase 1b (Jun N-terminal kinase-3).";
RL   J. Neurochem. 76:316-320(2001).
RN   [82]
RP   CLEAVAGE AT LEU-720.
RX   PubMed=11851430; DOI=10.1021/bi015794o;
RA   Weidemann A., Eggert S., Reinhard F.B.M., Vogel M., Paliga K.,
RA   Baier G., Masters C.L., Beyreuther K., Evin G.;
RT   "A novel epsilon-cleavage within the transmembrane domain of the
RT   Alzheimer amyloid precursor protein demonstrates homology with Notch
RT   processing.";
RL   Biochemistry 41:2825-2835(2002).
RN   [83]
RP   PHOSPHORYLATION AT TYR-757, INTERACTION WITH SHC1, AND MUTAGENESIS OF
RP   THR-743 AND TYR-757.
RX   PubMed=11877420; DOI=10.1074/jbc.M110286200;
RA   Tarr P.E., Roncarati R., Pelicci G., Pelicci P.G., D'Adamio L.;
RT   "Tyrosine phosphorylation of the beta-amyloid precursor protein
RT   cytoplasmic tail promotes interaction with Shc.";
RL   J. Biol. Chem. 277:16798-16804(2002).
RN   [84]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-542.
RC   TISSUE=Plasma;
RX   PubMed=16335952; DOI=10.1021/pr0502065;
RA   Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E.,
RA   Moore R.J., Smith R.D.;
RT   "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT   hydrazide chemistry, and mass spectrometry.";
RL   J. Proteome Res. 4:2070-2080(2005).
RN   [85]
RP   SIGNAL SEQUENCE CLEAVAGE SITE, AND TOPOLOGY.
RX   PubMed=2900137;
RA   Dyrks T., Weidemann A., Multhaup G., Salbaum J.M., Lemaire H.-G.,
RA   Kang J., Mueller-Hill B., Masters C.L., Beyreuther K.;
RT   "Identification, transmembrane orientation and biogenesis of the
RT   amyloid A4 precursor of Alzheimer's disease.";
RL   EMBO J. 7:949-957(1988).
RN   [86]
RP   REVIEW.
RX   PubMed=12142279; DOI=10.1146/annurev.cellbio.18.020402.142302;
RA   Annaert W., De Strooper B.;
RT   "A cell biological perspective on Alzheimer's disease.";
RL   Annu. Rev. Cell Dev. Biol. 18:25-51(2002).
RN   [87]
RP   INTERACTION WITH SORL1, AND SUBCELLULAR LOCATION.
RX   PubMed=16174740; DOI=10.1073/pnas.0503689102;
RA   Andersen O.M., Reiche J., Schmidt V., Gotthardt M., Spoelgen R.,
RA   Behlke J., von Arnim C.A., Breiderhoff T., Jansen P., Wu X.,
RA   Bales K.R., Cappai R., Masters C.L., Gliemann J., Mufson E.J.,
RA   Hyman B.T., Paul S.M., Nykjaer A., Willnow T.E.;
RT   "Neuronal sorting protein-related receptor sorLA/LR11 regulates
RT   processing of the amyloid precursor protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13461-13466(2005).
RN   [88]
RP   INTERACTION WITH APBB1.
RX   PubMed=18468999; DOI=10.1074/jbc.M801827200;
RA   Nakaya T., Kawai T., Suzuki T.;
RT   "Regulation of FE65 nuclear translocation and function by amyloid
RT   beta-protein precursor in osmotically stressed cells.";
RL   J. Biol. Chem. 283:19119-19131(2008).
RN   [89]
RP   INTERACTION WITH ITM2C.
RX   PubMed=19366692; DOI=10.1074/jbc.M109.006403;
RA   Matsuda S., Matsuda Y., D'Adamio L.;
RT   "BRI3 inhibits amyloid precursor protein processing in a
RT   mechanistically distinct manner from its homologue dementia gene
RT   BRI2.";
RL   J. Biol. Chem. 284:15815-15825(2009).
RN   [90]
RP   FUNCTION, CLEAVAGE, AND INTERACTION WITH TNFRSF21.
RX   PubMed=19225519; DOI=10.1038/nature07767;
RA   Nikolaev A., McLaughlin T., O'Leary D.D.M., Tessier-Lavigne M.;
RT   "APP binds DR6 to trigger axon pruning and neuron death via distinct
RT   caspases.";
RL   Nature 457:981-989(2009).
RN   [91]
RP   FUNCTION, AND INTERACTION WITH AGER.
RX   PubMed=19901339; DOI=10.1073/pnas.0905686106;
RA   Takuma K., Fang F., Zhang W., Yan S., Fukuzaki E., Du H., Sosunov A.,
RA   McKhann G., Funatsu Y., Nakamichi N., Nagai T., Mizoguchi H., Ibi D.,
RA   Hori O., Ogawa S., Stern D.M., Yamada K., Yan S.S.;
RT   "RAGE-mediated signaling contributes to intraneuronal transport of
RT   amyloid-{beta} and neuronal dysfunction.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:20021-20026(2009).
RN   [92]
RP   INTERACTION WITH GSAP.
RX   PubMed=20811458; DOI=10.1038/nature09325;
RA   He G., Luo W., Li P., Remmers C., Netzer W.J., Hendrick J.,
RA   Bettayeb K., Flajolet M., Gorelick F., Wennogle L.P., Greengard P.;
RT   "Gamma-secretase activating protein is a therapeutic target for
RT   Alzheimer's disease.";
RL   Nature 467:95-98(2010).
RN   [93]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [94]
RP   GLYCOSYLATION AT THR-633; THR-651; THR-652; SER-656; THR-663 AND
RP   SER-667 PROTEOLYTIC PROCESSING, STRUCTURE OF CARBOHYDRATES, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=21712440; DOI=10.1073/pnas.1102664108;
RA   Halim A., Brinkmalm G., Ruetschi U., Westman-Brinkmalm A.,
RA   Portelius E., Zetterberg H., Blennow K., Larson G., Nilsson J.;
RT   "Site-specific characterization of threonine, serine, and tyrosine
RT   glycosylations of amyloid precursor protein/amyloid beta-peptides in
RT   human cerebrospinal fluid.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:11848-11853(2011).
RN   [95]
RP   INTERACTION WITH S100A9.
RX   PubMed=22457725; DOI=10.1371/journal.pone.0032953;
RA   Zhang C., Liu Y., Gilthorpe J., van der Maarel J.R.;
RT   "MRP14 (S100A9) protein interacts with Alzheimer beta-amyloid peptide
RT   and induces its fibrillization.";
RL   PLoS ONE 7:E32953-E32953(2012).
RN   [96]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 287-344.
RX   PubMed=2125487; DOI=10.1021/bi00495a002;
RA   Hynes T.R., Randal M., Kennedy L.A., Eigenbrot C., Kossiakof A.A.;
RT   "X-ray crystal structure of the protease inhibitor domain of
RT   Alzheimer's amyloid beta-protein precursor.";
RL   Biochemistry 29:10018-10022(1990).
RN   [97]
RP   STRUCTURE BY NMR OF 289-344.
RX   PubMed=1718421; DOI=10.1021/bi00107a015;
RA   Heald S.L., Tilton R.F. Jr., Hammond L.S., Lee A., Bayney R.M.,
RA   Kamarck M.E., Ramabhadran T.V., Dreyer R.N., Davis G., Unterbeck A.,
RA   Tamburini P.P.;
RT   "Sequential NMR resonance assignment and structure determination of
RT   the Kunitz-type inhibitor domain of the Alzheimer's beta-amyloid
RT   precursor protein.";
RL   Biochemistry 30:10467-10478(1991).
RN   [98]
RP   STRUCTURE BY NMR OF 672-699.
RX   PubMed=7516706; DOI=10.1021/bi00191a006;
RA   Talafous J., Marcinowski K.J., Klopman G., Zagorski M.G.;
RT   "Solution structure of residues 1-28 of the amyloid beta-peptide.";
RL   Biochemistry 33:7788-7796(1994).
RN   [99]
RP   STRUCTURE BY NMR OF 672-711.
RX   PubMed=7588758; DOI=10.1111/j.1432-1033.1995.293_1.x;
RA   Sticht H., Bayer P., Willbold D., Dames S., Hilbich C., Beyreuther K.,
RA   Frank R.W., Rosch P.;
RT   "Structure of amyloid A4-(1-40)-peptide of Alzheimer's disease.";
RL   Eur. J. Biochem. 233:293-298(1995).
RN   [100]
RP   STRUCTURE BY NMR OF 696-706.
RX   PubMed=8973180; DOI=10.1021/bi961598j;
RA   Kohno T., Kobayashi K., Maeda T., Sato K., Takashima A.;
RT   "Three-dimensional structures of the amyloid beta peptide (25-35) in
RT   membrane-mimicking environment.";
RL   Biochemistry 35:16094-16104(1996).
RN   [101]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF KUNITZ DOMAIN IN COMPLEX WITH
RP   CHYMOTRYPSIN; TRYPSIN AND BASIC PANCREATIC TRYPSIN INHIBITOR.
RX   PubMed=9300481; DOI=10.1002/pro.5560060902;
RA   Scheidig A.J., Hynes T.R., Pelletier L.A., Wells J.A.,
RA   Kossiakoff A.A.;
RT   "Crystal structures of bovine chymotrypsin and trypsin complexed to
RT   the inhibitor domain of Alzheimer's amyloid beta-protein precursor
RT   (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of
RT   inhibitors with altered specificities.";
RL   Protein Sci. 6:1806-1824(1997).
RN   [102]
RP   STRUCTURE BY NMR OF 672-711.
RX   PubMed=9693002; DOI=10.1021/bi972979f;
RA   Coles M., Bicknell W., Watson A.A., Fairlie D.P., Craik D.J.;
RT   "Solution structure of amyloid beta-peptide(1-40) in a water-micelle
RT   environment. Is the membrane-spanning domain where we think it is?";
RL   Biochemistry 37:11064-11077(1998).
RN   [103]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 28-123.
RX   PubMed=10201399; DOI=10.1038/7562;
RA   Rossjohn J., Cappai R., Feil S.C., Henry A., McKinstry W.J.,
RA   Galatis D., Hesse L., Multhaup G., Beyreuther K., Masters C.L.,
RA   Parker M.W.;
RT   "Crystal structure of the N-terminal, growth factor-like domain of
RT   Alzheimer amyloid precursor protein.";
RL   Nat. Struct. Biol. 6:327-331(1999).
RN   [104]
RP   STRUCTURE OF CAA-APP VARIANTS.
RX   PubMed=10821838; DOI=10.1074/jbc.M003154200;
RA   Miravalle L., Tokuda T., Chiarle R., Giaccone G., Bugiani O.,
RA   Tagliavini F., Frangione B., Ghiso J.;
RT   "Substitutions at codon 22 of Alzheimer's Abeta peptide induce diverse
RT   conformational changes and apoptotic effects in human cerebral
RT   endothelial cells.";
RL   J. Biol. Chem. 275:27110-27116(2000).
RN   [105]
RP   STRUCTURE BY NMR OF 681-706.
RX   PubMed=10940221; DOI=10.1006/jsbi.2000.4288;
RA   Zhang S., Iwata K., Lachenmann M.J., Peng J.W., Li S., Stimson E.R.,
RA   Lu Y., Felix A.M., Maggio J.E., Lee J.P.;
RT   "The Alzheimer's peptide a beta adopts a collapsed coil structure in
RT   water.";
RL   J. Struct. Biol. 130:130-141(2000).
RN   [106]
RP   STRUCTURE BY NMR OF 672-699.
RX   PubMed=10940222; DOI=10.1006/jsbi.2000.4267;
RA   Poulsen S.-A., Watson A.A., Craik D.J.;
RT   "Solution structures in aqueous SDS micelles of two amyloid beta
RT   peptides of Abeta(1-28) mutated at the alpha-secretase cleavage
RT   site.";
RL   J. Struct. Biol. 130:142-152(2000).
RN   [107]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 346-551, PARTIAL PROTEIN
RP   SEQUENCE, MUTAGENESIS OF ARG-499 AND LYS-503, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=15304215; DOI=10.1016/j.molcel.2004.06.037;
RA   Wang Y., Ha Y.;
RT   "The X-ray structure of an antiparallel dimer of the human amyloid
RT   precursor protein E2 domain.";
RL   Mol. Cell 15:343-353(2004).
RN   [108]
RP   X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 672-711 IN COMPLEX WITH IDE.
RX   PubMed=17051221; DOI=10.1038/nature05143;
RA   Shen Y., Joachimiak A., Rosner M.R., Tang W.-J.;
RT   "Structures of human insulin-degrading enzyme reveal a new substrate
RT   recognition mechanism.";
RL   Nature 443:870-874(2006).
RN   [109]
RP   X-RAY CRYSTALLOGRAPHY (0.85 ANGSTROMS) OF 133-189, AND DISULFIDE
RP   BONDS.
RX   PubMed=17909280; DOI=10.1107/S1744309107041139;
RA   Kong G.K., Adams J.J., Cappai R., Parker M.W.;
RT   "Structure of Alzheimer's disease amyloid precursor protein copper-
RT   binding domain at atomic resolution.";
RL   Acta Crystallogr. F 63:819-824(2007).
RN   [110]
RP   X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 133-189 IN COMPLEXES WITH
RP   COPPER IONS, AND DISULFIDE BONDS.
RX   PubMed=17239395; DOI=10.1016/j.jmb.2006.12.041;
RA   Kong G.K., Adams J.J., Harris H.H., Boas J.F., Curtain C.C.,
RA   Galatis D., Masters C.L., Barnham K.J., McKinstry W.J., Cappai R.,
RA   Parker M.W.;
RT   "Structural studies of the Alzheimer's amyloid precursor protein
RT   copper-binding domain reveal how it binds copper ions.";
RL   J. Mol. Biol. 367:148-161(2007).
RN   [111]
RP   X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 672-679 IN COMPLEX WITH IGG.
RX   PubMed=17895381; DOI=10.1073/pnas.0705888104;
RA   Gardberg A.S., Dice L.T., Ou S., Rich R.L., Helmbrecht E., Ko J.,
RA   Wetzel R., Myszka D.G., Patterson P.H., Dealwis C.;
RT   "Molecular basis for passive immunotherapy of Alzheimer's disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:15659-15664(2007).
RN   [112]
RP   X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 672-678 IN COMPLEXES WITH
RP   ANTIBODY FAB FRAGMENTS.
RX   PubMed=19923222; DOI=10.1074/jbc.M109.045187;
RA   Basi G.S., Feinberg H., Oshidari F., Anderson J., Barbour R.,
RA   Baker J., Comery T.A., Diep L., Gill D., Johnson-Wood K., Goel A.,
RA   Grantcharova K., Lee M., Li J., Partridge A., Griswold-Prenner I.,
RA   Piot N., Walker D., Widom A., Pangalos M.N., Seubert P.,
RA   Jacobsen J.S., Schenk D., Weis W.I.;
RT   "Structural correlates of antibodies associated with acute reversal of
RT   amyloid beta-related behavioral deficits in a mouse model of Alzheimer
RT   disease.";
RL   J. Biol. Chem. 285:3417-3427(2010).
RN   [113]
RP   X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 18-190, PARTIAL PROTEIN
RP   SEQUENCE, SUBUNIT, DISULFIDE BONDS, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RX   PubMed=20212142; DOI=10.1073/pnas.0911326107;
RA   Dahms S.O., Hoefgen S., Roeser D., Schlott B., Guhrs K.H., Than M.E.;
RT   "Structure and biochemical analysis of the heparin-induced E1 dimer of
RT   the amyloid precursor protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:5381-5386(2010).
RN   [114]
RP   REVIEW ON VARIANTS.
RX   PubMed=1363811; DOI=10.1038/ng0792-233;
RA   Hardy J.;
RT   "Framing beta-amyloid.";
RL   Nat. Genet. 1:233-234(1992).
RN   [115]
RP   VARIANT CAA-APP GLN-693.
RX   PubMed=2111584; DOI=10.1126/science.2111584;
RA   Levy E., Carman M.D., Fernandez-Madrid I.J., Power M.D.,
RA   Lieberburg I., van Duinen S.G., Bots G.T.A.M., Luyendijk W.,
RA   Frangione B.;
RT   "Mutation of the Alzheimer's disease amyloid gene in hereditary
RT   cerebral hemorrhage, Dutch type.";
RL   Science 248:1124-1126(1990).
RN   [116]
RP   VARIANT AD1 ILE-717.
RX   PubMed=1671712; DOI=10.1038/349704a0;
RA   Goate A., Chartier-Harlin M.-C., Mullan M., Brown J., Crawford F.,
RA   Fidani L., Giuffra L., Haynes A., Irving N., James L., Mant R.,
RA   Newton P., Rooke K., Roques P., Talbot C., Pericak-Vance M.,
RA   Roses A.D., Williamson R., Rossor M., Owen M., Hardy J.;
RT   "Segregation of a missense mutation in the amyloid precursor protein
RT   gene with familial Alzheimer's disease.";
RL   Nature 349:704-706(1991).
RN   [117]
RP   VARIANT AD1 ILE-717.
RX   PubMed=1908231; DOI=10.1016/0006-291X(91)91011-Z;
RA   Yoshioka K., Miki T., Katsuya T., Ogihara T., Sakaki Y.;
RT   "The 717Val-->Ile substitution in amyloid precursor protein is
RT   associated with familial Alzheimer's disease regardless of ethnic
RT   groups.";
RL   Biochem. Biophys. Res. Commun. 178:1141-1146(1991).
RN   [118]
RP   VARIANT AD1 ILE-717.
RX   PubMed=1678058; DOI=10.1016/0140-6736(91)91612-X;
RA   Naruse S., Igarashi S., Kobayashi H., Aoki K., Inuzuka T., Kaneko K.,
RA   Shimizu T., Iihara K., Kojima T., Miyatake T., Tsuji S.;
RT   "Mis-sense mutation Val->Ile in exon 17 of amyloid precursor protein
RT   gene in Japanese familial Alzheimer's disease.";
RL   Lancet 337:978-979(1991).
RN   [119]
RP   VARIANT AD1 GLY-717.
RX   PubMed=1944558; DOI=10.1038/353844a0;
RA   Chartier-Harlin M.-C., Crawford F., Houlden H., Warren A., Hughes D.,
RA   Fidani L., Goate A., Rossor M., Roques P., Hardy J., Mullan M.;
RT   "Early-onset Alzheimer's disease caused by mutations at codon 717 of
RT   the beta-amyloid precursor protein gene.";
RL   Nature 353:844-846(1991).
RN   [120]
RP   VARIANT AD1 PHE-717.
RX   PubMed=1925564; DOI=10.1126/science.1925564;
RA   Murrell J.R., Farlow M., Ghetti B., Benson M.D.;
RT   "A mutation in the amyloid precursor protein associated with
RT   hereditary Alzheimer's disease.";
RL   Science 254:97-99(1991).
RN   [121]
RP   VARIANT AD1 GLY-693.
RX   PubMed=1415269;
RA   Kamino K., Orr H.T., Payami H., Wijsman E.M., Alonso M.E., Pulst S.M.,
RA   Anderson L., O'Dahl S., Nemens E., White J.A., Sadovnick A.D.,
RA   Ball M.J., Kaye J., Warren A., McInnis M.G., Antonarakis S.E.,
RA   Korenberg J.R., Sharma V., Kukull W., Larson E., Heston L.L.,
RA   Martin G.M., Bird T.D., Schellenberg G.D.;
RT   "Linkage and mutational analysis of familial Alzheimer disease
RT   kindreds for the APP gene region.";
RL   Am. J. Hum. Genet. 51:998-1014(1992).
RN   [122]
RP   VARIANT AD1 GLY-692.
RX   PubMed=1303239; DOI=10.1038/ng0692-218;
RA   Hendriks L., van Duijn C.M., Cras P., Cruts M., Van Hul W.,
RA   van Harskamp F., Warren A., McInnis M.G., Antonarakis S.E.,
RA   Martin J.J., Hofman A., Van Broeckhoven C.;
RT   "Presenile dementia and cerebral haemorrhage linked to a mutation at
RT   codon 692 of the beta-amyloid precursor protein gene.";
RL   Nat. Genet. 1:218-221(1992).
RN   [123]
RP   VARIANT AD1 670-ASN-LEU-671.
RX   PubMed=1302033; DOI=10.1038/ng0892-345;
RA   Mullan M., Crawford F., Axelman K., Houlden H., Lilius L., Winblad B.,
RA   Lannfelt L.;
RT   "A pathogenic mutation for probable Alzheimer's disease in the APP
RT   gene at the N-terminus of beta-amyloid.";
RL   Nat. Genet. 1:345-347(1992).
RN   [124]
RP   VARIANT VAL-713.
RX   PubMed=1307241; DOI=10.1038/ng0792-306;
RA   Jones C.T., Morris S., Yates C.M., Moffoot A., Sharpe C.,
RA   Brock D.J.H., St Clair D.;
RT   "Mutation in codon 713 of the beta amyloid precursor protein gene
RT   presenting with schizophrenia.";
RL   Nat. Genet. 1:306-309(1992).
RN   [125]
RP   VARIANT AD1 THR-713.
RX   PubMed=1303275; DOI=10.1038/ng1292-255;
RA   Carter D.A., Desmarais E., Bellis M., Campion D., Clerget-Darpoux F.,
RA   Brice A., Agid Y., Jaillard-Serradt A., Mallet J.;
RT   "More missense in amyloid gene.";
RL   Nat. Genet. 2:255-256(1992).
RN   [126]
RP   VARIANTS AD1 ILE-717 AND PHE-717.
RX   PubMed=8267572; DOI=10.1006/bbrc.1993.2491;
RA   Liepnieks J.J., Ghetti B., Farlow M., Roses A.D., Benson M.D.;
RT   "Characterization of amyloid fibril beta-peptide in familial
RT   Alzheimer's disease with APP717 mutations.";
RL   Biochem. Biophys. Res. Commun. 197:386-392(1993).
RN   [127]
RP   VARIANT ASP-665.
RX   PubMed=8154870; DOI=10.1002/ana.410350410;
RA   Peacock M.L., Murman D.L., Sima A.A.F., Warren J.T. Jr., Roses A.D.,
RA   Fink J.K.;
RT   "Novel amyloid precursor protein gene mutation (codon 665Asp) in a
RT   patient with late-onset Alzheimer's disease.";
RL   Ann. Neurol. 35:432-438(1994).
RN   [128]
RP   VARIANT AD1 PHE-717.
RX   PubMed=8290042; DOI=10.1212/WNL.44.1.105;
RA   Farlow M., Murrell J., Ghetti B., Unverzagt F., Zeldenrust S.,
RA   Benson M.D.;
RT   "Clinical characteristics in a kindred with early-onset Alzheimer's
RT   disease and their linkage to a G-->T change at position 2149 of the
RT   amyloid precursor protein gene.";
RL   Neurology 44:105-111(1994).
RN   [129]
RP   VARIANT AD1 ILE-717.
RX   PubMed=8577393; DOI=10.1016/0304-3940(95)12046-7;
RA   Brooks W.S., Martins R.N., De Voecht J., Nicholson G.A.,
RA   Schofield P.R., Kwok J.B.J., Fisher C., Yeung L.U.,
RA   Van Broeckhoven C.;
RT   "A mutation in codon 717 of the amyloid precursor protein gene in an
RT   Australian family with Alzheimer's disease.";
RL   Neurosci. Lett. 199:183-186(1995).
RN   [130]
RP   VARIANT AD1 VAL-716.
RX   PubMed=9328472; DOI=10.1093/hmg/6.12.2087;
RA   Eckman C.B., Mehta N.D., Crook R., Perez-Tur J., Prihar G.,
RA   Pfeiffer E., Graff-Radford N., Hinder P., Yager D., Zenk B.,
RA   Refolo L.M., Prada C.M., Younkin S.G., Hutton M., Hardy J.;
RT   "A new pathogenic mutation in the APP gene (I716V) increases the
RT   relative proportion of A beta 42(43).";
RL   Hum. Mol. Genet. 6:2087-2089(1997).
RN   [131]
RP   VARIANT AD1 GLY-692, AND CHARACTERIZATION OF PHENOTYPE.
RX   PubMed=9754958; DOI=10.1007/s004010050892;
RA   Cras P., van Harskamp F., Hendriks L., Ceuterick C., van Duijn C.M.,
RA   Stefanko S.Z., Hofman A., Kros J.M., Van Broeckhoven C., Martin J.J.;
RT   "Presenile Alzheimer dementia characterized by amyloid angiopathy and
RT   large amyloid core type senile plaques in the APP 692Ala-->Gly
RT   mutation.";
RL   Acta Neuropathol. 96:253-260(1998).
RN   [132]
RP   VARIANT AD1 MET-715, AND CHARACTERIZATION OF VARIANT AD1 MET-715.
RX   PubMed=10097173; DOI=10.1073/pnas.96.7.4119;
RA   Ancolio K., Dumanchin C., Barelli H., Warter J.-M., Brice A.,
RA   Campion D., Frebourg T., Checler F.;
RT   "Unusual phenotypic alteration of beta amyloid precursor protein
RT   (betaAPP) maturation by a new Val-715 --> Met betaAPP-770 mutation
RT   responsible for probable early-onset Alzheimer's disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:4119-4124(1999).
RN   [133]
RP   VARIANT AD1 ILE-717.
RX   PubMed=10631141; DOI=10.1086/302702;
RA   Finckh U., Mueller-Thomsen T., Mann U., Eggers C., Marksteiner J.,
RA   Meins W., Binetti G., Alberici A., Hock C., Nitsch R.M., Gal A.;
RT   "High prevalence of pathogenic mutations in patients with early-onset
RT   dementia detected by sequence analyses of four different genes.";
RL   Am. J. Hum. Genet. 66:110-117(2000).
RN   [134]
RP   VARIANT AD1 PRO-723.
RX   PubMed=10665499;
RX   DOI=10.1002/1531-8249(200002)47:2<249::AID-ANA18>3.0.CO;2-8;
RA   Kwok J.B.J., Li Q.X., Hallupp M., Whyte S., Ames D., Beyreuther K.,
RA   Masters C.L., Schofield P.R.;
RT   "Novel Leu723Pro amyloid precursor protein mutation increases amyloid
RT   beta42(43) peptide levels and induces apoptosis.";
RL   Ann. Neurol. 47:249-253(2000).
RN   [135]
RP   VARIANT AD1 LEU-717.
RX   PubMed=10867787; DOI=10.1001/archneur.57.6.885;
RA   Murrell J.R., Hake A.M., Quaid K.A., Farlow M.R., Ghetti B.;
RT   "Early-onset Alzheimer disease caused by a new mutation (V717L) in the
RT   amyloid precursor protein gene.";
RL   Arch. Neurol. 57:885-887(2000).
RN   [136]
RP   VARIANT AD1 ILE-714, CHARACTERIZATION OF VARIANT AD1 ILE-714, AND
RP   MUTAGENESIS OF VAL-717.
RX   PubMed=11063718; DOI=10.1093/hmg/9.18.2589;
RA   Kumar-Singh S., De Jonghe C., Cruts M., Kleinert R., Wang R.,
RA   Mercken M., De Strooper B., Vanderstichele H., Loefgren A.,
RA   Vanderhoeven I., Backhovens H., Vanmechelen E., Kroisel P.M.,
RA   Van Broeckhoven C.;
RT   "Nonfibrillar diffuse amyloid deposition due to a gamma(42)-secretase
RT   site mutation points to an essential role for N-truncated A beta(42)
RT   in Alzheimer's disease.";
RL   Hum. Mol. Genet. 9:2589-2598(2000).
RN   [137]
RP   VARIANT CAA-APP ASN-694.
RX   PubMed=11409420; DOI=10.1002/ana.1009;
RA   Grabowski T.J., Cho H.S., Vonsattel J.P.G., Rebeck G.W.,
RA   Greenberg S.M.;
RT   "Novel amyloid precursor protein mutation in an Iowa family with
RT   dementia and severe cerebral amyloid angiopathy.";
RL   Ann. Neurol. 49:697-705(2001).
RN   [138]
RP   CHARACTERIZATION OF VARIANT AD1 GLY-692.
RX   PubMed=11311152;
RA   Walsh D.M., Hartley D.M., Condron M.M., Selkoe D.J., Teplow D.B.;
RT   "In vitro studies of amyloid beta-protein fibril assembly and toxicity
RT   provide clues to the aetiology of Flemish variant (Ala692-->Gly)
RT   Alzheimer's disease.";
RL   Biochem. J. 355:869-877(2001).
RN   [139]
RP   VARIANT AD1 GLY-693.
RX   PubMed=11528419; DOI=10.1038/nn0901-887;
RA   Nilsberth C., Westlind-Danielsson A., Eckman C.B., Condron M.M.,
RA   Axelman K., Forsell C., Stenh C., Luthman J., Teplow D.B.,
RA   Younkin S.G., Naeslund J., Lannfelt L.;
RT   "The 'Arctic' APP mutation (E693G) causes Alzheimer's disease by
RT   enhanced Abeta protofibril formation.";
RL   Nat. Neurosci. 4:887-893(2001).
RN   [140]
RP   VARIANT AD1 ALA-714.
RX   PubMed=12034808; DOI=10.1212/WNL.58.10.1574;
RA   Pasalar P., Najmabadi H., Noorian A.R., Moghimi B., Jannati A.,
RA   Soltanzadeh A., Krefft T., Crook R., Hardy J.;
RT   "An Iranian family with Alzheimer's disease caused by a novel APP
RT   mutation (Thr714Ala).";
RL   Neurology 58:1574-1575(2002).
RN   [141]
RP   VARIANT CAA-APP ASN-694.
RX   PubMed=12654973; DOI=10.1212/01.WNL.0000050140.10044.A8;
RA   Greenberg S.M., Shin Y., Grabowski T.J., Cooper G.E., Rebeck G.W.,
RA   Iglesias S., Chapon F., Tournier-Lasserve E., Baron J.-C.;
RT   "Hemorrhagic stroke associated with the Iowa amyloid precursor protein
RT   mutation.";
RL   Neurology 60:1020-1022(2003).
RN   [142]
RP   VARIANT AD1 THR-713.
RX   PubMed=15365148; DOI=10.1212/01.WNL.0000137048.80666.86;
RA   Rossi G., Giaccone G., Maletta R., Morbin M., Capobianco R.,
RA   Mangieri M., Giovagnoli A.R., Bizzi A., Tomaino C., Perri M.,
RA   Di Natale M., Tagliavini F., Bugiani O., Bruni A.C.;
RT   "A family with Alzheimer disease and strokes associated with A713T
RT   mutation of the APP gene.";
RL   Neurology 63:910-912(2004).
RN   [143]
RP   VARIANT CAA-APP VAL-705.
RX   PubMed=16178030; DOI=10.1002/ana.20571;
RA   Obici L., Demarchi A., de Rosa G., Bellotti V., Marciano S.,
RA   Donadei S., Arbustini E., Palladini G., Diegoli M., Genovese E.,
RA   Ferrari G., Coverlizza S., Merlini G.;
RT   "A novel AbetaPP mutation exclusively associated with cerebral amyloid
RT   angiopathy.";
RL   Ann. Neurol. 58:639-644(2005).
RN   [144]
RP   VARIANT AD1 ILE-714.
RX   PubMed=15668448; DOI=10.1212/01.WNL.0000149761.70566.3E;
RA   Edwards-Lee T., Ringman J.M., Chung J., Werner J., Morgan A.,
RA   St George-Hyslop P.H., Thompson P., Dutton R., Mlikotic A.,
RA   Rogaeva E., Hardy J.;
RT   "An African American family with early-onset Alzheimer disease and an
RT   APP (T714I) mutation.";
RL   Neurology 64:377-379(2005).
CC   -!- FUNCTION: Functions as a cell surface receptor and performs
CC       physiological functions on the surface of neurons relevant to
CC       neurite growth, neuronal adhesion and axonogenesis. Involved in
CC       cell mobility and transcription regulation through protein-protein
CC       interactions. Can promote transcription activation through binding
CC       to APBB1-KAT5 and inhibits Notch signaling through interaction
CC       with Numb. Couples to apoptosis-inducing pathways such as those
CC       mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By
CC       similarity). Acts as a kinesin I membrane receptor, mediating the
CC       axonal transport of beta-secretase and presenilin 1. Involved in
CC       copper homeostasis/oxidative stress through copper ion reduction.
CC       In vitro, copper-metallated APP induces neuronal death directly or
CC       is potentiated through Cu(2+)-mediated low-density lipoprotein
CC       oxidation. Can regulate neurite outgrowth through binding to
CC       components of the extracellular matrix such as heparin and
CC       collagen I and IV. The splice isoforms that contain the BPTI
CC       domain possess protease inhibitor activity. Induces a AGER-
CC       dependent pathway that involves activation of p38 MAPK, resulting
CC       in internalization of amyloid-beta peptide and leading to
CC       mitochondrial dysfunction in cultured cortical neurons. Provides
CC       Cu(2+) ions for GPC1 which are required for release of nitric
CC       oxide (NO) and subsequent degradation of the heparan sulfate
CC       chains on GPC1.
CC   -!- FUNCTION: Beta-amyloid peptides are lipophilic metal chelators
CC       with metal-reducing activity. Bind transient metals such as
CC       copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to
CC       Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more
CC       effective reductant than beta-amyloid 40. Beta-amyloid peptides
CC       bind to lipoproteins and apolipoproteins E and J in the CSF and to
CC       HDL particles in plasma, inhibiting metal-catalyzed oxidation of
CC       lipoproteins. Beta-APP42 may activate mononuclear phagocytes in
CC       the brain and elicit inflammatory responses. Promotes both tau
CC       aggregation and TPK II-mediated phosphorylation. Interaction with
CC       Also bind GPC1 in lipid rafts.
CC   -!- FUNCTION: Appicans elicit adhesion of neural cells to the
CC       extracellular matrix and may regulate neurite outgrowth in the
CC       brain (By similarity).
CC   -!- FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved
CC       peptides, including C31, are potent enhancers of neuronal
CC       apoptosis.
CC   -!- FUNCTION: N-APP binds TNFRSF21 triggering caspase activation and
CC       degeneration of both neuronal cell bodies (via caspase-3) and
CC       axons (via caspase-6).
CC   -!- SUBUNIT: Binds, via its C-terminus, to the PID domain of several
CC       cytoplasmic proteins, including APBB family members, the APBA
CC       family, MAPK8IP1, SHC1 and, NUMB and DAB1 (By similarity). Binding
CC       to DAB1 inhibits its serine phosphorylation (By similarity).
CC       Interacts (via NPXY motif) with DAB2 (via PID domain); the
CC       interaction is impaired by tyrosine phosphorylation of the NPXY
CC       motif. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1,
CC       IB1, KNS2 (via its TPR domains) (By similarity), APPBP2 (via BaSS)
CC       and DDB1. In vitro, it binds MAPT via the MT-binding domains (By
CC       similarity). Associates with microtubules in the presence of ATP
CC       and in a kinesin-dependent manner (By similarity). Interacts,
CC       through a C-terminal domain, with GNAO1. Amyloid beta-42 binds
CC       CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2.
CC       Soluble APP binds, via its N-terminal head, to FBLN1. Interacts
CC       with CPEB1 and AGER (By similarity). Interacts with ANKS1B and
CC       TNFRSF21. Interacts with ITM2B. Interacts with ITM2C. Interacts
CC       with IDE. Can form homodimers; this is promoted by heparin
CC       binding. Beta-amyloid protein 40 interacts with S100A9. CTF-alpha
CC       product of APP interacts with GSAP. Interacts with SORL1.
CC   -!- INTERACTION:
CC       Self; NbExp=79; IntAct=EBI-77613, EBI-77613;
CC       Q306T3:- (xeno); NbExp=3; IntAct=EBI-77613, EBI-8294101;
CC       P31696:AGRN (xeno); NbExp=3; IntAct=EBI-2431589, EBI-457650;
CC       Q02410:APBA1; NbExp=3; IntAct=EBI-77613, EBI-368690;
CC       O00213:APBB1; NbExp=5; IntAct=EBI-77613, EBI-81694;
CC       Q92870:APBB2; NbExp=2; IntAct=EBI-77613, EBI-79277;
CC       P51693:APLP1; NbExp=2; IntAct=EBI-302641, EBI-74648;
CC       Q06481:APLP2; NbExp=2; IntAct=EBI-302641, EBI-79306;
CC       P02647:APOA1; NbExp=5; IntAct=EBI-77613, EBI-701692;
CC       Q13867:BLMH; NbExp=2; IntAct=EBI-302641, EBI-718504;
CC       P15253:CALR (xeno); NbExp=3; IntAct=EBI-77613, EBI-9005200;
CC       Q8K3H7:CALR (xeno); NbExp=2; IntAct=EBI-3894543, EBI-9005068;
CC       P39060:COL18A1; NbExp=2; IntAct=EBI-821758, EBI-2566375;
CC       P07339:CTSD; NbExp=2; IntAct=EBI-77613, EBI-2115097;
CC       O75955:FLOT1; NbExp=5; IntAct=EBI-77613, EBI-603643;
CC       P01100:FOS; NbExp=3; IntAct=EBI-77613, EBI-852851;
CC       P46089:GPR3; NbExp=2; IntAct=EBI-302641, EBI-3909653;
CC       Q9NSC5:HOMER3; NbExp=3; IntAct=EBI-302661, EBI-748420;
CC       Q99714:HSD17B10; NbExp=4; IntAct=EBI-77613, EBI-79964;
CC       O43736:ITM2A; NbExp=3; IntAct=EBI-302641, EBI-2431769;
CC       P05412:JUN; NbExp=2; IntAct=EBI-77613, EBI-852823;
CC       P10636:MAPT; NbExp=9; IntAct=EBI-77613, EBI-366182;
CC       Q93074:MED12; NbExp=2; IntAct=EBI-77613, EBI-394357;
CC       P03897:MT-ND3; NbExp=2; IntAct=EBI-821758, EBI-1246249;
CC       P21359:NF1; NbExp=3; IntAct=EBI-77613, EBI-1172917;
CC       P08138:NGFR; NbExp=2; IntAct=EBI-77613, EBI-1387782;
CC       P07174:Ngfr (xeno); NbExp=2; IntAct=EBI-2431589, EBI-1038810;
CC       P61457:PCBD1; NbExp=2; IntAct=EBI-77613, EBI-740475;
CC       Q15113:PCOLCE; NbExp=3; IntAct=EBI-821758, EBI-8869614;
CC       P30101:PDIA3; NbExp=3; IntAct=EBI-77613, EBI-979862;
CC       Q13526:PIN1; NbExp=2; IntAct=EBI-302641, EBI-714158;
CC       P04156:PRNP; NbExp=3; IntAct=EBI-77613, EBI-977302;
CC       P49768:PSEN1; NbExp=6; IntAct=EBI-77613, EBI-297277;
CC       P29353:SHC1; NbExp=5; IntAct=EBI-77613, EBI-78835;
CC       Q92529:SHC3; NbExp=2; IntAct=EBI-77613, EBI-79084;
CC       Q9NP59:SLC40A1; NbExp=4; IntAct=EBI-77613, EBI-725153;
CC       Q8BGY9:Slc5a7 (xeno); NbExp=2; IntAct=EBI-77613, EBI-2010752;
CC       Q9HCB6:SPON1; NbExp=3; IntAct=EBI-302641, EBI-2431846;
CC       P01137:TGFB1; NbExp=2; IntAct=EBI-77613, EBI-779636;
CC       P61812:TGFB2; NbExp=6; IntAct=EBI-77613, EBI-779581;
CC       O75509:TNFRSF21; NbExp=3; IntAct=EBI-77613, EBI-2313231;
CC       Q13625:TP53BP2; NbExp=3; IntAct=EBI-77613, EBI-77642;
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane
CC       protein. Membrane, clathrin-coated pit. Note=Cell surface protein
CC       that rapidly becomes internalized via clathrin-coated pits. During
CC       maturation, the immature APP (N-glycosylated in the endoplasmic
CC       reticulum) moves to the Golgi complex where complete maturation
CC       occurs (O-glycosylated and sulfated). After alpha-secretase
CC       cleavage, soluble APP is released into the extracellular space and
CC       the C-terminal is internalized to endosomes and lysosomes. Some
CC       APP accumulates in secretory transport vesicles leaving the late
CC       Golgi compartment and returns to the cell surface. Gamma-CTF(59)
CC       peptide is located to both the cytoplasm and nuclei of neurons. It
CC       can be translocated to the nucleus through association with APBB1
CC       (Fe65). Beta-APP42 associates with FRPL1 at the cell surface and
CC       the complex is then rapidly internalized. APP sorts to the
CC       basolateral surface in epithelial cells. During neuronal
CC       differentiation, the Thr-743 phosphorylated form is located mainly
CC       in growth cones, moderately in neurites and sparingly in the cell
CC       body. Casein kinase phosphorylation can occur either at the cell
CC       surface or within a post-Golgi compartment. Associates with GPC1
CC       in perinuclear compartments. Colocalizes with SORL1 in a vesicular
CC       pattern in cytoplasm and perinuclear regions.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=11;
CC         Comment=Additional isoforms seem to exist. Experimental
CC         confirmation may be lacking for some isoforms;
CC       Name=APP770; Synonyms=PreA4 770;
CC         IsoId=P05067-1; Sequence=Displayed;
CC         Note=A major isoform;
CC       Name=APP305;
CC         IsoId=P05067-2; Sequence=VSP_000005, VSP_000006;
CC       Name=L-APP677;
CC         IsoId=P05067-3; Sequence=VSP_000002, VSP_000004, VSP_000009;
CC         Note=The L-isoforms are referred to as appicans;
CC       Name=APP695; Synonyms=PreA4 695;
CC         IsoId=P05067-4; Sequence=VSP_000002, VSP_000004;
CC         Note=A major isoform;
CC       Name=L-APP696;
CC         IsoId=P05067-5; Sequence=VSP_000002, VSP_000003, VSP_000009;
CC         Note=The L-isoforms are referred to as appicans;
CC       Name=APP714;
CC         IsoId=P05067-6; Sequence=VSP_000002, VSP_000003;
CC       Name=L-APP733;
CC         IsoId=P05067-7; Sequence=VSP_000007, VSP_000008, VSP_000009;
CC         Note=The L-isoforms are referred to as appicans;
CC       Name=APP751; Synonyms=PreA4 751;
CC         IsoId=P05067-8; Sequence=VSP_000007, VSP_000008;
CC         Note=A major isoform;
CC       Name=L-APP752;
CC         IsoId=P05067-9; Sequence=VSP_000009;
CC       Name=APP639;
CC         IsoId=P05067-10; Sequence=VSP_009116, VSP_009117, VSP_009118;
CC       Name=11;
CC         IsoId=P05067-11; Sequence=VSP_045446, VSP_045447;
CC   -!- TISSUE SPECIFICITY: Expressed in all fetal tissues examined with
CC       highest levels in brain, kidney, heart and spleen. Weak expression
CC       in liver. In adult brain, highest expression found in the frontal
CC       lobe of the cortex and in the anterior perisylvian cortex-
CC       opercular gyri. Moderate expression in the cerebellar cortex, the
CC       posterior perisylvian cortex-opercular gyri and the temporal
CC       associated cortex. Weak expression found in the striate, extra-
CC       striate and motor cortices. Expressed in cerebrospinal fluid, and
CC       plasma. Isoform APP695 is the predominant form in neuronal tissue,
CC       isoform APP751 and isoform APP770 are widely expressed in non-
CC       neuronal cells. Isoform APP751 is the most abundant form in T-
CC       lymphocytes. Appican is expressed in astrocytes.
CC   -!- INDUCTION: Increased levels during neuronal differentiation.
CC   -!- DOMAIN: The basolateral sorting signal (BaSS) is required for
CC       sorting of membrane proteins to the basolateral surface of
CC       epithelial cells.
CC   -!- DOMAIN: The NPXY sequence motif found in many tyrosine-
CC       phosphorylated proteins is required for the specific binding of
CC       the PID domain. However, additional amino acids either N- or C-
CC       terminal to the NPXY motif are often required for complete
CC       interaction. The PID domain-containing proteins which bind APP
CC       require the YENPTY motif for full interaction. These interactions
CC       are independent of phosphorylation on the terminal tyrosine
CC       residue. The NPXY site is also involved in clathrin-mediated
CC       endocytosis.
CC   -!- PTM: Proteolytically processed under normal cellular conditions.
CC       Cleavage either by alpha-secretase, beta-secretase or theta-
CC       secretase leads to generation and extracellular release of soluble
CC       APP peptides, S-APP-alpha and S-APP-beta, and the retention of
CC       corresponding membrane-anchored C-terminal fragments, C80, C83 and
CC       C99. Subsequent processing of C80 and C83 by gamma-secretase
CC       yields P3 peptides. This is the major secretory pathway and is
CC       non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated
CC       gamma-secretase processing of C99 releases the amyloid beta
CC       proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42),
CC       major components of amyloid plaques, and the cytotoxic C-terminal
CC       fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). Many
CC       other minor beta-amyloid peptides, beta-amyloid 1-X peptides, are
CC       found in cerebral spinal fluid (CSF) including the beta-amyloid X-
CC       15 peptides, produced from the cleavage by alpha-secretase and all
CC       terminatiing at Gln-686.
CC   -!- PTM: Proteolytically cleaved by caspases during neuronal
CC       apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9
CC       results in the production of the neurotoxic C31 peptide and the
CC       increased production of beta-amyloid peptides.
CC   -!- PTM: N- and O-glycosylated. O-glycosylation on Ser and Thr
CC       residues with core 1 or possibly core 8 glycans. Partial tyrosine
CC       glycosylation (Tyr-681) is found on some minor, short beta-amyloid
CC       peptides (beta-amyloid 1-15, 1-16, 1-17, 1-18, 1-19 and 1-20) but
CC       not found on beta-amyloid 38, beta-amyloid 40 nor on beta-amyloid
CC       42. Modification on a tyrosine is unusual and is more prevelant in
CC       AD patients. Glycans had Neu5AcHex(Neu5Ac)HexNAc-O-Tyr,
CC       Neu5AcNeu5AcHex(Neu5Ac)HexNAc-O-Tyr and O-
CC       AcNeu5AcNeu5AcHex(Neu5Ac)HexNAc-O-Tyr structures, where O-Ac is O-
CC       acetylation of Neu5Ac. Neu5AcNeu5Ac is most likely Neu5Ac
CC       2,8Neu5Ac linked. O-glycosylations in the vicinity of the cleavage
CC       sites may influence the proteolytic processing. Appicans are L-APP
CC       isoforms with O-linked chondroitin sulfate.
CC   -!- PTM: Phosphorylation in the C-terminal on tyrosine, threonine and
CC       serine residues is neuron-specific. Phosphorylation can affect APP
CC       processing, neuronal differentiation and interaction with other
CC       proteins. Phosphorylated on Thr-743 in neuronal cells by Cdc5
CC       kinase and Mapk10, in dividing cells by Cdc2 kinase in a cell-
CC       cycle dependent manner with maximal levels at the G2/M phase and,
CC       in vitro, by GSK-3-beta. The Thr-743 phosphorylated form causes a
CC       conformational change which reduces binding of Fe65 family
CC       members. Phosphorylation on Tyr-757 is required for SHC binding.
CC       Phosphorylated in the extracellular domain by casein kinases on
CC       both soluble and membrane-bound APP. This phosphorylation is
CC       inhibited by heparin.
CC   -!- PTM: Extracellular binding and reduction of copper, results in a
CC       corresponding oxidation of Cys-144 and Cys-158, and the formation
CC       of a disulfide bond. In vitro, the APP-Cu(+) complex in the
CC       presence of hydrogen peroxide results in an increased production
CC       of beta-amyloid-containing peptides.
CC   -!- PTM: Trophic-factor deprivation triggers the cleavage of surface
CC       APP by beta-secretase to release sAPP-beta which is further
CC       cleaved to release an N-terminal fragment of APP (N-APP).
CC   -!- PTM: Beta-amyloid peptides are degraded by IDE.
CC   -!- MASS SPECTROMETRY: Mass=6461.6; Method=MALDI; Range=712-767;
CC       Source=PubMed:12214090;
CC   -!- MASS SPECTROMETRY: Mass=6451.6; Method=MALDI; Range=714-770;
CC       Source=PubMed:12214090;
CC   -!- MASS SPECTROMETRY: Mass=6436.8; Method=MALDI; Range=715-769;
CC       Source=PubMed:12214090;
CC   -!- MASS SPECTROMETRY: Mass=5752.5; Method=MALDI; Range=719-767;
CC       Source=PubMed:12214090;
CC   -!- DISEASE: Alzheimer disease 1 (AD1) [MIM:104300]: A familial early-
CC       onset form of Alzheimer disease. It can be associated with
CC       cerebral amyloid angiopathy. Alzheimer disease is a
CC       neurodegenerative disorder characterized by progressive dementia,
CC       loss of cognitive abilities, and deposition of fibrillar amyloid
CC       proteins as intraneuronal neurofibrillary tangles, extracellular
CC       amyloid plaques and vascular amyloid deposits. The major
CC       constituent of these plaques is the neurotoxic amyloid-beta-APP
CC       40-42 peptide (s), derived proteolytically from the transmembrane
CC       precursor protein APP by sequential secretase processing. The
CC       cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved
CC       products such as C31 derived from APP, are also implicated in
CC       neuronal death. Note=The disease is caused by mutations affecting
CC       the gene represented in this entry.
CC   -!- DISEASE: Cerebral amyloid angiopathy, APP-related (CAA-APP)
CC       [MIM:605714]: A hereditary localized amyloidosis due to amyloid-
CC       beta A4 peptide(s) deposition in the cerebral vessels. The
CC       principal clinical characteristics are recurrent cerebral and
CC       cerebellar hemorrhages, recurrent strokes, cerebral ischemia,
CC       cerebral infarction, and progressive mental deterioration.
CC       Patients develop cerebral hemorrhage because of the severe
CC       cerebral amyloid angiopathy. Parenchymal amyloid deposits are rare
CC       and largely in the form of pre-amyloid lesions or diffuse plaque-
CC       like structures. They are Congo red negative and lack the dense
CC       amyloid cores commonly present in Alzheimer disease. Some affected
CC       individuals manifest progressive aphasic dementia,
CC       leukoencephalopathy, and occipital calcifications. Note=The
CC       disease is caused by mutations affecting the gene represented in
CC       this entry.
CC   -!- MISCELLANEOUS: Chelation of metal ions, notably copper, iron and
CC       zinc, can induce histidine-bridging between beta-amyloid molecules
CC       resulting in beta-amyloid-metal aggregates. The affinity for
CC       copper is much higher than for other transient metals and is
CC       increased under acidic conditions. Extracellular zinc-binding
CC       increases binding of heparin to APP and inhibits collagen-binding.
CC   -!- SIMILARITY: Belongs to the APP family.
CC   -!- SIMILARITY: Contains 1 BPTI/Kunitz inhibitor domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA58727.1; Type=Miscellaneous discrepancy; Note=Contamination by an Alu repeat;
CC   -!- WEB RESOURCE: Name=Alzheimer Research Forum; Note=APP mutations;
CC       URL="http://www.alzforum.org/res/com/mut/app/default.asp";
CC   -!- WEB RESOURCE: Name=AD mutations;
CC       URL="http://www.molgen.ua.ac.be/ADmutations/";
CC   -!- WEB RESOURCE: Name=NIEHS-SNPs;
CC       URL="http://egp.gs.washington.edu/data/app/";
CC   -!- WEB RESOURCE: Name=Wikipedia; Note=Amyloid beta entry;
CC       URL="http://en.wikipedia.org/wiki/Amyloid_beta";
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DR   EMBL; Y00264; CAA68374.1; -; mRNA.
DR   EMBL; X13466; CAA31830.1; -; Genomic_DNA.
DR   EMBL; X13467; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13468; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13469; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13470; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13471; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13472; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13473; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13474; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13475; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13476; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13477; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13478; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13479; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13487; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X13488; CAA31830.1; JOINED; Genomic_DNA.
DR   EMBL; X06989; CAA30050.1; -; mRNA.
DR   EMBL; M33112; AAB59502.1; -; Genomic_DNA.
DR   EMBL; M34862; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34863; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34864; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34865; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34866; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34867; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34868; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34869; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34870; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34871; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34872; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34873; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34874; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34876; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34877; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34878; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34879; AAB59502.1; JOINED; Genomic_DNA.
DR   EMBL; M34875; AAB59501.1; ALT_TERM; Genomic_DNA.
DR   EMBL; M34862; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34863; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34864; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34865; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34866; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34867; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34868; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34869; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34870; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34871; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34872; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; M34873; AAB59501.1; JOINED; Genomic_DNA.
DR   EMBL; D87675; BAA22264.1; -; Genomic_DNA.
DR   EMBL; AK312326; BAG35248.1; -; mRNA.
DR   EMBL; AK295621; BAG58500.1; -; mRNA.
DR   EMBL; AY919674; AAW82435.1; -; Genomic_DNA.
DR   EMBL; AP001439; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP001440; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP001441; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP001442; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP001443; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471079; EAX09958.1; -; Genomic_DNA.
DR   EMBL; CH471079; EAX09959.1; -; Genomic_DNA.
DR   EMBL; CH471079; EAX09960.1; -; Genomic_DNA.
DR   EMBL; CH471079; EAX09961.1; -; Genomic_DNA.
DR   EMBL; CH471079; EAX09963.1; -; Genomic_DNA.
DR   EMBL; CH471079; EAX09965.1; -; Genomic_DNA.
DR   EMBL; BC004369; AAH04369.1; -; mRNA.
DR   EMBL; BC065529; AAH65529.1; -; mRNA.
DR   EMBL; M35675; AAA60163.1; ALT_SEQ; mRNA.
DR   EMBL; M24547; AAC13654.1; -; Genomic_DNA.
DR   EMBL; M24546; AAC13654.1; JOINED; Genomic_DNA.
DR   EMBL; M28373; AAA58727.1; ALT_SEQ; mRNA.
DR   EMBL; X06982; CAA30042.1; -; mRNA.
DR   EMBL; X06981; CAA30041.1; -; mRNA.
DR   EMBL; M18734; AAA51726.1; -; mRNA.
DR   EMBL; M29270; AAA51768.1; -; Genomic_DNA.
DR   EMBL; M29269; AAA51768.1; JOINED; Genomic_DNA.
DR   EMBL; AB066441; BAB71958.2; -; mRNA.
DR   EMBL; M15533; AAA35540.1; -; mRNA.
DR   EMBL; M15532; AAA51564.1; -; mRNA.
DR   EMBL; M37896; AAA51727.1; -; Genomic_DNA.
DR   EMBL; M37895; AAA51727.1; JOINED; Genomic_DNA.
DR   EMBL; S45136; AAB23646.1; -; Genomic_DNA.
DR   EMBL; S60317; AAC60601.2; -; Genomic_DNA.
DR   EMBL; AF282245; AAQ14327.1; -; mRNA.
DR   EMBL; S60721; AAB26263.2; -; mRNA.
DR   EMBL; S61380; AAB26264.2; -; mRNA.
DR   EMBL; S61383; AAB26265.2; -; mRNA.
DR   EMBL; M16765; AAA51722.1; -; mRNA.
DR   CCDS; CCDS13576.1; -. [P05067-1]
DR   CCDS; CCDS13577.1; -. [P05067-4]
DR   CCDS; CCDS33523.1; -. [P05067-8]
DR   CCDS; CCDS46638.1; -. [P05067-10]
DR   CCDS; CCDS56212.1; -. [P05067-11]
DR   CCDS; CCDS56213.1; -. [P05067-9]
DR   PIR; S01442; S01442.
DR   PIR; S02260; QRHUA4.
DR   RefSeq; NP_000475.1; NM_000484.3. [P05067-1]
DR   RefSeq; NP_001129488.1; NM_001136016.3. [P05067-11]
DR   RefSeq; NP_001129601.1; NM_001136129.2. [P05067-10]
DR   RefSeq; NP_001129602.1; NM_001136130.2.
DR   RefSeq; NP_001129603.1; NM_001136131.2.
DR   RefSeq; NP_001191230.1; NM_001204301.1. [P05067-9]
DR   RefSeq; NP_001191231.1; NM_001204302.1. [P05067-7]
DR   RefSeq; NP_001191232.1; NM_001204303.1. [P05067-3]
DR   RefSeq; NP_958816.1; NM_201413.2. [P05067-8]
DR   RefSeq; NP_958817.1; NM_201414.2. [P05067-4]
DR   UniGene; Hs.434980; -.
DR   PDB; 1AAP; X-ray; 1.50 A; A/B=287-344.
DR   PDB; 1AMB; NMR; -; A=672-699.
DR   PDB; 1AMC; NMR; -; A=672-699.
DR   PDB; 1AML; NMR; -; A=672-711.
DR   PDB; 1BA4; NMR; -; A=672-711.
DR   PDB; 1BA6; NMR; -; A=672-711.
DR   PDB; 1BJB; NMR; -; A=672-699.
DR   PDB; 1BJC; NMR; -; A=672-699.
DR   PDB; 1BRC; X-ray; 2.50 A; I=287-342.
DR   PDB; 1CA0; X-ray; 2.10 A; D/I=289-342.
DR   PDB; 1HZ3; NMR; -; A=681-706.
DR   PDB; 1IYT; NMR; -; A=672-713.
DR   PDB; 1MWP; X-ray; 1.80 A; A=28-123.
DR   PDB; 1OWT; NMR; -; A=124-189.
DR   PDB; 1QCM; NMR; -; A=696-706.
DR   PDB; 1QWP; NMR; -; A=696-706.
DR   PDB; 1QXC; NMR; -; A=696-706.
DR   PDB; 1QYT; NMR; -; A=696-706.
DR   PDB; 1TAW; X-ray; 1.80 A; B=287-344.
DR   PDB; 1TKN; NMR; -; A=460-569.
DR   PDB; 1UO7; Model; -; A=672-713.
DR   PDB; 1UO8; Model; -; A=672-713.
DR   PDB; 1UOA; Model; -; A=672-713.
DR   PDB; 1UOI; Model; -; A=672-713.
DR   PDB; 1X11; X-ray; 2.50 A; C/D=754-766.
DR   PDB; 1Z0Q; NMR; -; A=672-713.
DR   PDB; 1ZE7; NMR; -; A=672-687.
DR   PDB; 1ZE9; NMR; -; A=672-687.
DR   PDB; 1ZJD; X-ray; 2.60 A; B=289-344.
DR   PDB; 2BEG; NMR; -; A/B/C/D/E=672-713.
DR   PDB; 2BOM; Model; -; A/B=681-713.
DR   PDB; 2BP4; NMR; -; A=672-687.
DR   PDB; 2FJZ; X-ray; 1.61 A; A=133-189.
DR   PDB; 2FK1; X-ray; 1.60 A; A=133-189.
DR   PDB; 2FK2; X-ray; 1.65 A; A=133-189.
DR   PDB; 2FK3; X-ray; 2.40 A; A/B/C/D/E/F/G/H=133-189.
DR   PDB; 2FKL; X-ray; 2.50 A; A/B=124-189.
DR   PDB; 2FMA; X-ray; 0.85 A; A=133-189.
DR   PDB; 2G47; X-ray; 2.10 A; C/D=672-711.
DR   PDB; 2IPU; X-ray; 1.65 A; P/Q=672-679.
DR   PDB; 2LFM; NMR; -; A=672-711.
DR   PDB; 2LLM; NMR; -; A=686-726.
DR   PDB; 2LMN; NMR; -; A/B/C/D/E/F/G/H/I/J/K/L=672-711.
DR   PDB; 2LMO; NMR; -; A/B/C/D/E/F/G/H/I/J/K/L=672-711.
DR   PDB; 2LMP; NMR; -; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=672-711.
DR   PDB; 2LMQ; NMR; -; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=672-711.
DR   PDB; 2LNQ; NMR; -; A/B/C/D/E/F/G/H=672-711.
DR   PDB; 2LOH; NMR; -; A/B=686-726.
DR   PDB; 2LP1; NMR; -; A=671-770.
DR   PDB; 2LZ3; NMR; -; A/B=699-726.
DR   PDB; 2LZ4; NMR; -; A/B=699-726.
DR   PDB; 2M4J; NMR; -; A/B/C/D/E/F/G/H/I=672-711.
DR   PDB; 2M9R; NMR; -; A=672-711.
DR   PDB; 2M9S; NMR; -; A=672-711.
DR   PDB; 2OTK; NMR; -; C=672-711.
DR   PDB; 2R0W; X-ray; 2.50 A; Q=672-679.
DR   PDB; 2WK3; X-ray; 2.59 A; C/D=672-713.
DR   PDB; 2Y29; X-ray; 2.30 A; A=687-692.
DR   PDB; 2Y2A; X-ray; 1.91 A; A=687-692.
DR   PDB; 2Y3J; X-ray; 1.99 A; A/B/C/D/E/F/G/H=701-706.
DR   PDB; 2Y3K; X-ray; 1.90 A; A/B/C/D/E/F/G/H=706-713.
DR   PDB; 2Y3L; X-ray; 2.10 A; A/B/C/G=706-713.
DR   PDB; 3AYU; X-ray; 2.00 A; B=586-595.
DR   PDB; 3BAE; X-ray; 1.59 A; A=672-699.
DR   PDB; 3BKJ; X-ray; 1.59 A; A=672-687.
DR   PDB; 3DXC; X-ray; 2.10 A; B/D=739-770.
DR   PDB; 3DXD; X-ray; 2.20 A; B/D=739-770.
DR   PDB; 3DXE; X-ray; 2.00 A; B/D=739-770.
DR   PDB; 3GCI; X-ray; 2.04 A; P=707-713.
DR   PDB; 3IFL; X-ray; 1.50 A; P=672-678.
DR   PDB; 3IFN; X-ray; 1.50 A; P=672-711.
DR   PDB; 3IFO; X-ray; 2.15 A; P/Q=672-678.
DR   PDB; 3IFP; X-ray; 2.95 A; P/Q/R/S=672-678.
DR   PDB; 3JQ5; X-ray; 2.03 A; B=672-679.
DR   PDB; 3JQL; X-ray; 1.20 A; B=687-692.
DR   PDB; 3JTI; X-ray; 1.80 A; B=699-706.
DR   PDB; 3KTM; X-ray; 2.70 A; A/B/C/D/E/F/G/H=18-190.
DR   PDB; 3L33; X-ray; 2.48 A; E/F/G/H=290-341.
DR   PDB; 3L81; X-ray; 1.60 A; B=761-767.
DR   PDB; 3MOQ; X-ray; 2.05 A; A/B/C/D=689-712.
DR   PDB; 3MXC; X-ray; 2.00 A; L=754-762.
DR   PDB; 3MXY; X-ray; 2.30 A; L=754-762.
DR   PDB; 3NYJ; X-ray; 3.20 A; A=365-567.
DR   PDB; 3NYL; X-ray; 2.80 A; A=365-570.
DR   PDB; 3OVJ; X-ray; 1.80 A; A/B/C/D=687-692.
DR   PDB; 3OW9; X-ray; 1.80 A; A/B=687-692.
DR   PDB; 3SV1; X-ray; 3.30 A; D/E/F=754-767.
DR   PDB; 3U0T; X-ray; 2.50 A; E/F=701-711.
DR   PDB; 3UMH; X-ray; 2.00 A; A=370-575.
DR   PDB; 3UMI; X-ray; 2.40 A; A=370-575.
DR   PDB; 3UMK; X-ray; 2.60 A; A=370-575.
DR   PDB; 4HIX; X-ray; 2.20 A; A=672-699.
DR   PDB; 4MDR; X-ray; 1.85 A; B=758-767.
DR   PDB; 4NGE; X-ray; 2.70 A; B/E=672-711.
DR   PDBsum; 1AAP; -.
DR   PDBsum; 1AMB; -.
DR   PDBsum; 1AMC; -.
DR   PDBsum; 1AML; -.
DR   PDBsum; 1BA4; -.
DR   PDBsum; 1BA6; -.
DR   PDBsum; 1BJB; -.
DR   PDBsum; 1BJC; -.
DR   PDBsum; 1BRC; -.
DR   PDBsum; 1CA0; -.
DR   PDBsum; 1HZ3; -.
DR   PDBsum; 1IYT; -.
DR   PDBsum; 1MWP; -.
DR   PDBsum; 1OWT; -.
DR   PDBsum; 1QCM; -.
DR   PDBsum; 1QWP; -.
DR   PDBsum; 1QXC; -.
DR   PDBsum; 1QYT; -.
DR   PDBsum; 1TAW; -.
DR   PDBsum; 1TKN; -.
DR   PDBsum; 1UO7; -.
DR   PDBsum; 1UO8; -.
DR   PDBsum; 1UOA; -.
DR   PDBsum; 1UOI; -.
DR   PDBsum; 1X11; -.
DR   PDBsum; 1Z0Q; -.
DR   PDBsum; 1ZE7; -.
DR   PDBsum; 1ZE9; -.
DR   PDBsum; 1ZJD; -.
DR   PDBsum; 2BEG; -.
DR   PDBsum; 2BOM; -.
DR   PDBsum; 2BP4; -.
DR   PDBsum; 2FJZ; -.
DR   PDBsum; 2FK1; -.
DR   PDBsum; 2FK2; -.
DR   PDBsum; 2FK3; -.
DR   PDBsum; 2FKL; -.
DR   PDBsum; 2FMA; -.
DR   PDBsum; 2G47; -.
DR   PDBsum; 2IPU; -.
DR   PDBsum; 2LFM; -.
DR   PDBsum; 2LLM; -.
DR   PDBsum; 2LMN; -.
DR   PDBsum; 2LMO; -.
DR   PDBsum; 2LMP; -.
DR   PDBsum; 2LMQ; -.
DR   PDBsum; 2LNQ; -.
DR   PDBsum; 2LOH; -.
DR   PDBsum; 2LP1; -.
DR   PDBsum; 2LZ3; -.
DR   PDBsum; 2LZ4; -.
DR   PDBsum; 2M4J; -.
DR   PDBsum; 2M9R; -.
DR   PDBsum; 2M9S; -.
DR   PDBsum; 2OTK; -.
DR   PDBsum; 2R0W; -.
DR   PDBsum; 2WK3; -.
DR   PDBsum; 2Y29; -.
DR   PDBsum; 2Y2A; -.
DR   PDBsum; 2Y3J; -.
DR   PDBsum; 2Y3K; -.
DR   PDBsum; 2Y3L; -.
DR   PDBsum; 3AYU; -.
DR   PDBsum; 3BAE; -.
DR   PDBsum; 3BKJ; -.
DR   PDBsum; 3DXC; -.
DR   PDBsum; 3DXD; -.
DR   PDBsum; 3DXE; -.
DR   PDBsum; 3GCI; -.
DR   PDBsum; 3IFL; -.
DR   PDBsum; 3IFN; -.
DR   PDBsum; 3IFO; -.
DR   PDBsum; 3IFP; -.
DR   PDBsum; 3JQ5; -.
DR   PDBsum; 3JQL; -.
DR   PDBsum; 3JTI; -.
DR   PDBsum; 3KTM; -.
DR   PDBsum; 3L33; -.
DR   PDBsum; 3L81; -.
DR   PDBsum; 3MOQ; -.
DR   PDBsum; 3MXC; -.
DR   PDBsum; 3MXY; -.
DR   PDBsum; 3NYJ; -.
DR   PDBsum; 3NYL; -.
DR   PDBsum; 3OVJ; -.
DR   PDBsum; 3OW9; -.
DR   PDBsum; 3SV1; -.
DR   PDBsum; 3U0T; -.
DR   PDBsum; 3UMH; -.
DR   PDBsum; 3UMI; -.
DR   PDBsum; 3UMK; -.
DR   PDBsum; 4HIX; -.
DR   PDBsum; 4MDR; -.
DR   PDBsum; 4NGE; -.
DR   ProteinModelPortal; P05067; -.
DR   SMR; P05067; 26-192, 287-342, 385-567, 683-728, 741-768.
DR   BioGrid; 106848; 1971.
DR   DIP; DIP-574N; -.
DR   IntAct; P05067; 113.
DR   MINT; MINT-150767; -.
DR   BindingDB; P05067; -.
DR   ChEMBL; CHEMBL2487; -.
DR   MEROPS; I02.015; -.
DR   TCDB; 1.C.50.1.2; the amyloid -protein peptide (app) family.
DR   PhosphoSite; P05067; -.
DR   UniCarbKB; P05067; -.
DR   DMDM; 112927; -.
DR   SWISS-2DPAGE; P05067; -.
DR   MaxQB; P05067; -.
DR   PaxDb; P05067; -.
DR   PRIDE; P05067; -.
DR   DNASU; 351; -.
DR   Ensembl; ENST00000346798; ENSP00000284981; ENSG00000142192. [P05067-1]
DR   Ensembl; ENST00000348990; ENSP00000345463; ENSG00000142192. [P05067-4]
DR   Ensembl; ENST00000354192; ENSP00000346129; ENSG00000142192. [P05067-10]
DR   Ensembl; ENST00000357903; ENSP00000350578; ENSG00000142192. [P05067-8]
DR   Ensembl; ENST00000358918; ENSP00000351796; ENSG00000142192. [P05067-9]
DR   Ensembl; ENST00000359726; ENSP00000352760; ENSG00000142192. [P05067-6]
DR   Ensembl; ENST00000440126; ENSP00000387483; ENSG00000142192. [P05067-11]
DR   GeneID; 351; -.
DR   KEGG; hsa:351; -.
DR   UCSC; uc002ylz.3; human. [P05067-1]
DR   UCSC; uc002yma.3; human. [P05067-8]
DR   UCSC; uc002ymb.3; human. [P05067-4]
DR   UCSC; uc010glj.3; human. [P05067-10]
DR   UCSC; uc010glk.3; human.
DR   UCSC; uc011acj.2; human. [P05067-2]
DR   UCSC; uc021whz.1; human. [P05067-9]
DR   UCSC; uc021wia.1; human. [P05067-7]
DR   UCSC; uc021wib.1; human. [P05067-3]
DR   CTD; 351; -.
DR   GeneCards; GC21M027252; -.
DR   GeneReviews; APP; -.
DR   HGNC; HGNC:620; APP.
DR   HPA; CAB000157; -.
DR   HPA; HPA001462; -.
DR   MIM; 104300; phenotype.
DR   MIM; 104760; gene.
DR   MIM; 605714; phenotype.
DR   neXtProt; NX_P05067; -.
DR   Orphanet; 1020; Early-onset autosomal dominant Alzheimer disease.
DR   Orphanet; 324723; Hereditary cerebral hemorrhage with amyloidosis, Arctic type.
DR   Orphanet; 100006; Hereditary cerebral hemorrhage with amyloidosis, Dutch type.
DR   Orphanet; 324718; Hereditary cerebral hemorrhage with amyloidosis, Flemish type.
DR   Orphanet; 324708; Hereditary cerebral hemorrhage with amyloidosis, Iowa type.
DR   Orphanet; 324713; Hereditary cerebral hemorrhage with amyloidosis, Italian type.
DR   Orphanet; 324703; Hereditary cerebral hemorrhage with amyloidosis, Piedmont type.
DR   PharmGKB; PA24910; -.
DR   eggNOG; NOG289770; -.
DR   HOVERGEN; HBG000051; -.
DR   InParanoid; P05067; -.
DR   KO; K04520; -.
DR   OMA; THAHIVI; -.
DR   OrthoDB; EOG7RNJZP; -.
DR   PhylomeDB; P05067; -.
DR   TreeFam; TF317274; -.
DR   BioCyc; MetaCyc:ENSG00000142192-MONOMER; -.
DR   Reactome; REACT_111102; Signal Transduction.
DR   Reactome; REACT_116125; Disease.
DR   Reactome; REACT_118779; Extracellular matrix organization.
DR   Reactome; REACT_604; Hemostasis.
DR   Reactome; REACT_6900; Immune System.
DR   SABIO-RK; P05067; -.
DR   ChiTaRS; app; human.
DR   EvolutionaryTrace; P05067; -.
DR   GeneWiki; Amyloid_precursor_protein; -.
DR   GenomeRNAi; 351; -.
DR   NextBio; 1445; -.
DR   PMAP-CutDB; P05067; -.
DR   PRO; PR:P05067; -.
DR   ArrayExpress; P05067; -.
DR   Bgee; P05067; -.
DR   Genevestigator; P05067; -.
DR   GO; GO:0045177; C:apical part of cell; IEA:Ensembl.
DR   GO; GO:0030424; C:axon; ISS:UniProtKB.
DR   GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR   GO; GO:0035253; C:ciliary rootlet; IEA:Ensembl.
DR   GO; GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0043198; C:dendritic shaft; IDA:MGI.
DR   GO; GO:0043197; C:dendritic spine; IDA:MGI.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0070062; C:extracellular vesicular exosome; IDA:UniProt.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; ISS:UniProtKB.
DR   GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR   GO; GO:0045121; C:membrane raft; IDA:ParkinsonsUK-UCL.
DR   GO; GO:0031594; C:neuromuscular junction; IEA:Ensembl.
DR   GO; GO:0005641; C:nuclear envelope lumen; IDA:Alzheimers_University_of_Toronto.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProt.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0031093; C:platelet alpha granule lumen; TAS:Reactome.
DR   GO; GO:0043235; C:receptor complex; IDA:MGI.
DR   GO; GO:0051233; C:spindle midzone; IEA:Ensembl.
DR   GO; GO:0045202; C:synapse; IDA:MGI.
DR   GO; GO:0033130; F:acetylcholine receptor binding; IPI:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0019899; F:enzyme binding; IPI:ParkinsonsUK-UCL.
DR   GO; GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0016504; F:peptidase activator activity; IEA:Ensembl.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0051425; F:PTB domain binding; IPI:BHF-UCL.
DR   GO; GO:0005102; F:receptor binding; IPI:BHF-UCL.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR   GO; GO:0008344; P:adult locomotory behavior; ISS:UniProtKB.
DR   GO; GO:0008088; P:axon cargo transport; ISS:UniProtKB.
DR   GO; GO:0016199; P:axon midline choice point recognition; ISS:UniProtKB.
DR   GO; GO:0007409; P:axonogenesis; ISS:UniProtKB.
DR   GO; GO:0007596; P:blood coagulation; TAS:Reactome.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   GO; GO:0006878; P:cellular copper ion homeostasis; ISS:UniProtKB.
DR   GO; GO:0008203; P:cholesterol metabolic process; IEA:Ensembl.
DR   GO; GO:0048669; P:collateral sprouting in absence of injury; ISS:UniProtKB.
DR   GO; GO:0016358; P:dendrite development; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   GO; GO:0030198; P:extracellular matrix organization; ISS:UniProtKB.
DR   GO; GO:0030900; P:forebrain development; IEA:Ensembl.
DR   GO; GO:0045087; P:innate immune response; TAS:Reactome.
DR   GO; GO:0035235; P:ionotropic glutamate receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0007626; P:locomotory behavior; ISS:UniProtKB.
DR   GO; GO:0007617; P:mating behavior; ISS:UniProtKB.
DR   GO; GO:0000085; P:mitotic G2 phase; ISS:UniProtKB.
DR   GO; GO:0006378; P:mRNA polyadenylation; ISS:UniProtKB.
DR   GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:GOC.
DR   GO; GO:0045665; P:negative regulation of neuron differentiation; IEA:Ensembl.
DR   GO; GO:0050885; P:neuromuscular process controlling balance; IEA:Ensembl.
DR   GO; GO:0051402; P:neuron apoptotic process; IMP:UniProtKB.
DR   GO; GO:0031175; P:neuron projection development; ISS:UniProtKB.
DR   GO; GO:0016322; P:neuron remodeling; ISS:UniProtKB.
DR   GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0035872; P:nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway; TAS:Reactome.
DR   GO; GO:0030168; P:platelet activation; TAS:Reactome.
DR   GO; GO:0002576; P:platelet degranulation; TAS:Reactome.
DR   GO; GO:0010971; P:positive regulation of G2/M transition of mitotic cell cycle; IEA:Ensembl.
DR   GO; GO:0045931; P:positive regulation of mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Ensembl.
DR   GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
DR   GO; GO:0007176; P:regulation of epidermal growth factor-activated receptor activity; ISS:UniProtKB.
DR   GO; GO:0040014; P:regulation of multicellular organism growth; ISS:UniProtKB.
DR   GO; GO:0043393; P:regulation of protein binding; IEA:Ensembl.
DR   GO; GO:0050803; P:regulation of synapse structure and activity; ISS:UniProtKB.
DR   GO; GO:0006417; P:regulation of translation; ISS:UniProtKB.
DR   GO; GO:0006979; P:response to oxidative stress; IEA:Ensembl.
DR   GO; GO:0051563; P:smooth endoplasmic reticulum calcium ion homeostasis; IEA:Ensembl.
DR   GO; GO:0001967; P:suckling behavior; IEA:Ensembl.
DR   GO; GO:0051124; P:synaptic growth at neuromuscular junction; IEA:Ensembl.
DR   GO; GO:0008542; P:visual learning; ISS:UniProtKB.
DR   Gene3D; 3.30.1490.140; -; 1.
DR   Gene3D; 3.90.570.10; -; 1.
DR   Gene3D; 4.10.230.10; -; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR008155; Amyloid_glyco.
DR   InterPro; IPR013803; Amyloid_glyco_Abeta.
DR   InterPro; IPR011178; Amyloid_glyco_Cu-bd.
DR   InterPro; IPR024329; Amyloid_glyco_E2_domain.
DR   InterPro; IPR008154; Amyloid_glyco_extra.
DR   InterPro; IPR019744; Amyloid_glyco_extracell_CS.
DR   InterPro; IPR015849; Amyloid_glyco_heparin-bd.
DR   InterPro; IPR019745; Amyloid_glyco_intracell_CS.
DR   InterPro; IPR028866; APP.
DR   InterPro; IPR019543; APP_amyloid_C.
DR   InterPro; IPR002223; Prot_inh_Kunz-m.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   PANTHER; PTHR23103:SF7; PTHR23103:SF7; 1.
DR   Pfam; PF10515; APP_amyloid; 1.
DR   Pfam; PF12924; APP_Cu_bd; 1.
DR   Pfam; PF12925; APP_E2; 1.
DR   Pfam; PF02177; APP_N; 1.
DR   Pfam; PF03494; Beta-APP; 1.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00203; AMYLOIDA4.
DR   PRINTS; PR00759; BASICPTASE.
DR   PRINTS; PR00204; BETAAMYLOID.
DR   SMART; SM00006; A4_EXTRA; 1.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF109843; SSF109843; 1.
DR   SUPFAM; SSF56491; SSF56491; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   SUPFAM; SSF89811; SSF89811; 1.
DR   PROSITE; PS00319; A4_EXTRA; 1.
DR   PROSITE; PS00320; A4_INTRA; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Alzheimer disease; Amyloid;
KW   Amyloidosis; Apoptosis; Cell adhesion; Coated pit; Complete proteome;
KW   Copper; Direct protein sequencing; Disease mutation; Disulfide bond;
KW   Endocytosis; Glycoprotein; Heparin-binding; Iron; Membrane;
KW   Metal-binding; Neurodegeneration; Notch signaling pathway;
KW   Phosphoprotein; Polymorphism; Protease inhibitor; Proteoglycan;
KW   Reference proteome; Serine protease inhibitor; Signal; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   SIGNAL        1     17
FT   CHAIN        18    770       Amyloid beta A4 protein.
FT                                /FTId=PRO_0000000088.
FT   CHAIN        18    687       Soluble APP-alpha.
FT                                /FTId=PRO_0000000089.
FT   CHAIN        18    671       Soluble APP-beta.
FT                                /FTId=PRO_0000000090.
FT   CHAIN        18    286       N-APP.
FT                                /FTId=PRO_0000381966.
FT   CHAIN       672    770       C99.
FT                                /FTId=PRO_0000000091.
FT   CHAIN       672    713       Beta-amyloid protein 42.
FT                                /FTId=PRO_0000000092.
FT   CHAIN       672    711       Beta-amyloid protein 40.
FT                                /FTId=PRO_0000000093.
FT   CHAIN       688    770       C83.
FT                                /FTId=PRO_0000000094.
FT   PEPTIDE     688    713       P3(42).
FT                                /FTId=PRO_0000000095.
FT   PEPTIDE     688    711       P3(40).
FT                                /FTId=PRO_0000000096.
FT   CHAIN       691    770       C80.
FT                                /FTId=PRO_0000384574.
FT   CHAIN       712    770       Gamma-secretase C-terminal fragment 59.
FT                                /FTId=PRO_0000000097.
FT   CHAIN       714    770       Gamma-secretase C-terminal fragment 57.
FT                                /FTId=PRO_0000000098.
FT   CHAIN       721    770       Gamma-secretase C-terminal fragment 50
FT                                (By similarity).
FT                                /FTId=PRO_0000000099.
FT   CHAIN       740    770       C31.
FT                                /FTId=PRO_0000000100.
FT   TOPO_DOM     18    699       Extracellular (Potential).
FT   TRANSMEM    700    723       Helical; (Potential).
FT   TOPO_DOM    724    770       Cytoplasmic (Potential).
FT   DOMAIN      291    341       BPTI/Kunitz inhibitor.
FT   REGION       96    110       Heparin-binding.
FT   REGION      181    188       Zinc-binding.
FT   REGION      391    423       Heparin-binding.
FT   REGION      491    522       Heparin-binding.
FT   REGION      523    540       Collagen-binding.
FT   REGION      732    751       Interaction with G(o)-alpha.
FT   MOTIF       724    734       Basolateral sorting signal.
FT   MOTIF       759    762       NPXY motif; contains endocytosis signal.
FT   COMPBIAS    230    260       Asp/Glu-rich (acidic).
FT   COMPBIAS    274    280       Poly-Thr.
FT   METAL       147    147       Copper 1.
FT   METAL       151    151       Copper 1.
FT   METAL       168    168       Copper 1.
FT   METAL       677    677       Copper or zinc 2.
FT   METAL       681    681       Copper or zinc 2 (Probable).
FT   METAL       684    684       Copper or zinc 2.
FT   METAL       685    685       Copper or zinc 2.
FT   SITE        144    144       Required for Cu(2+) reduction.
FT   SITE        301    302       Reactive bond.
FT   SITE        671    672       Cleavage; by beta-secretase.
FT   SITE        672    673       Cleavage; by caspase-6; when associated
FT                                with variant 670-N-L-671.
FT   SITE        687    688       Cleavage; by alpha-secretase.
FT   SITE        690    691       Cleavage; by theta-secretase.
FT   SITE        704    704       Implicated in free radical propagation
FT                                (By similarity).
FT   SITE        706    706       Susceptible to oxidation.
FT   SITE        711    712       Cleavage; by gamma-secretase; site 1.
FT   SITE        713    714       Cleavage; by gamma-secretase; site 2.
FT   SITE        720    721       Cleavage; by gamma-secretase; site 3.
FT   SITE        739    740       Cleavage; by caspase-6, caspase-8 or
FT                                caspase-9.
FT   MOD_RES     198    198       Phosphoserine; by CK2.
FT   MOD_RES     206    206       Phosphoserine; by CK1.
FT   MOD_RES     729    729       Phosphothreonine (By similarity).
FT   MOD_RES     730    730       Phosphoserine; by APP-kinase I (By
FT                                similarity).
FT   MOD_RES     743    743       Phosphothreonine; by CDK5 and MAPK10.
FT   MOD_RES     757    757       Phosphotyrosine.
FT   CARBOHYD    542    542       N-linked (GlcNAc...).
FT   CARBOHYD    571    571       N-linked (GlcNAc...) (Probable).
FT   CARBOHYD    614    614       O-linked (GalNAc...).
FT   CARBOHYD    623    623       O-linked (GalNAc...).
FT   CARBOHYD    628    628       O-linked (GalNAc...).
FT   CARBOHYD    633    633       O-linked (GalNAc...).
FT   CARBOHYD    651    651       O-linked (GalNAc...).
FT   CARBOHYD    652    652       O-linked (GalNAc...).
FT   CARBOHYD    656    656       O-linked (Xyl...) (chondroitin sulfate);
FT                                in L-APP isoforms.
FT   CARBOHYD    659    659       O-linked (GalNAc...).
FT   CARBOHYD    663    663       O-linked (GalNAc...) (Probable).
FT   CARBOHYD    667    667       O-linked (GalNAc...) (Probable).
FT   CARBOHYD    679    679       O-linked (GalNAc...).
FT   CARBOHYD    697    697       O-linked (GalNAc...).
FT   DISULFID     38     62
FT   DISULFID     73    117
FT   DISULFID     98    105
FT   DISULFID    133    187
FT   DISULFID    144    174
FT   DISULFID    158    186
FT   DISULFID    291    341
FT   DISULFID    300    324
FT   DISULFID    316    337
FT   VAR_SEQ       1     19       MLPGLALLLLAAWTARALE -> MDQLEDLLVLFINY (in
FT                                isoform 11).
FT                                /FTId=VSP_045446.
FT   VAR_SEQ      19     74       Missing (in isoform APP639).
FT                                /FTId=VSP_009116.
FT   VAR_SEQ     289    363       Missing (in isoform APP639).
FT                                /FTId=VSP_009117.
FT   VAR_SEQ     289    289       E -> V (in isoform APP695, isoform L-
FT                                APP696, isoform L-APP677 and isoform
FT                                APP714).
FT                                /FTId=VSP_000002.
FT   VAR_SEQ     290    364       Missing (in isoform APP695 and isoform L-
FT                                APP677).
FT                                /FTId=VSP_000004.
FT   VAR_SEQ     290    345       Missing (in isoform L-APP696 and isoform
FT                                APP714).
FT                                /FTId=VSP_000003.
FT   VAR_SEQ     290    305       VCSEQAETGPCRAMIS -> KWYKEVHSGQARWLML (in
FT                                isoform APP305).
FT                                /FTId=VSP_000005.
FT   VAR_SEQ     306    770       Missing (in isoform APP305).
FT                                /FTId=VSP_000006.
FT   VAR_SEQ     345    364       MSQSLLKTTQEPLARDPVKL -> I (in isoform
FT                                11).
FT                                /FTId=VSP_045447.
FT   VAR_SEQ     345    345       M -> I (in isoform L-APP733 and isoform
FT                                APP751).
FT                                /FTId=VSP_000007.
FT   VAR_SEQ     346    364       Missing (in isoform L-APP733 and isoform
FT                                APP751).
FT                                /FTId=VSP_000008.
FT   VAR_SEQ     364    364       L -> V (in isoform APP639).
FT                                /FTId=VSP_009118.
FT   VAR_SEQ     637    654       Missing (in isoform L-APP677, isoform L-
FT                                APP696, isoform L-APP733 and isoform L-
FT                                APP752).
FT                                /FTId=VSP_000009.
FT   VARIANT     501    501       E -> K (in dbSNP:rs45588932).
FT                                /FTId=VAR_022315.
FT   VARIANT     665    665       E -> D (in a patient with late onset
FT                                Alzheimer disease).
FT                                /FTId=VAR_010107.
FT   VARIANT     670    671       KM -> NL (in AD1).
FT                                /FTId=VAR_000015.
FT   VARIANT     678    678       D -> N (in AD1).
FT                                /FTId=VAR_044424.
FT   VARIANT     692    692       A -> G (in AD1; Flemish mutation;
FT                                increases the solubility of processed
FT                                beta-amyloid peptides and increases the
FT                                stability of peptide oligomers).
FT                                /FTId=VAR_000016.
FT   VARIANT     693    693       E -> G (in AD1).
FT                                /FTId=VAR_014215.
FT   VARIANT     693    693       E -> K (in CAA-APP; Italian type).
FT                                /FTId=VAR_014216.
FT   VARIANT     693    693       E -> Q (in CAA-APP; Dutch type).
FT                                /FTId=VAR_000017.
FT   VARIANT     694    694       D -> N (in CAA-APP; Iowa type).
FT                                /FTId=VAR_014217.
FT   VARIANT     705    705       L -> V (in CAA-APP; Italian type).
FT                                /FTId=VAR_032276.
FT   VARIANT     713    713       A -> T (in AD1).
FT                                /FTId=VAR_000019.
FT   VARIANT     713    713       A -> V (in one chronic schizophrenia
FT                                patient; unknown pathological
FT                                significance; dbSNP:rs1800557).
FT                                /FTId=VAR_000018.
FT   VARIANT     714    714       T -> A (in AD1).
FT                                /FTId=VAR_032277.
FT   VARIANT     714    714       T -> I (in AD1; increased beta-APP42/
FT                                beta-APP40 ratio).
FT                                /FTId=VAR_014218.
FT   VARIANT     715    715       V -> M (in AD1; decreased beta-APP40/
FT                                total APP-beta).
FT                                /FTId=VAR_010108.
FT   VARIANT     716    716       I -> V (in AD1).
FT                                /FTId=VAR_000020.
FT   VARIANT     717    717       V -> F (in AD1).
FT                                /FTId=VAR_000023.
FT   VARIANT     717    717       V -> G (in AD1).
FT                                /FTId=VAR_000022.
FT   VARIANT     717    717       V -> I (in AD1).
FT                                /FTId=VAR_000021.
FT   VARIANT     717    717       V -> L (in AD1).
FT                                /FTId=VAR_014219.
FT   VARIANT     723    723       L -> P (in AD1).
FT                                /FTId=VAR_010109.
FT   MUTAGEN      99    102       KRGR->NQGG: Reduced heparin-binding.
FT   MUTAGEN     137    137       H->N: Binds copper. Forms dimer.
FT   MUTAGEN     141    141       M->T: Binds copper. Forms dimer.
FT   MUTAGEN     144    144       C->S: Binds copper. No dimer formation.
FT                                No copper reducing activity.
FT   MUTAGEN     147    149       HLH->ALA: 50% decrease in copper reducing
FT                                activity.
FT   MUTAGEN     147    147       H->A: Some decrease in copper reducing
FT                                activity.
FT   MUTAGEN     147    147       H->N: Binds copper. Forms dimer.
FT   MUTAGEN     147    147       H->Y: Greatly reduced copper-mediated
FT                                low-density lipoprotein oxidation.
FT   MUTAGEN     151    151       H->K: Greatly reduced copper-mediated
FT                                low-density lipoprotein oxidation.
FT   MUTAGEN     151    151       H->N: Binds copper. Forms dimer.
FT   MUTAGEN     198    198       S->A: Greatly reduced casein kinase
FT                                phosphorylation.
FT   MUTAGEN     206    206       S->A: Reduced casein kinase
FT                                phosphorylation.
FT   MUTAGEN     499    499       R->A: Reduced affinity for heparin; when
FT                                associated with A-503.
FT   MUTAGEN     503    503       K->A: Reduced affinity for heparin; when
FT                                associated with A-499.
FT   MUTAGEN     656    656       S->A: Abolishes chondroitin sulfate
FT                                binding in L-APP733 isoform.
FT   MUTAGEN     676    676       R->G: 60-70% zinc-induced beta-APP (28)
FT                                peptide aggregation.
FT   MUTAGEN     681    681       Y->F: 60-70% zinc-induced beta-APP (28)
FT                                peptide aggregation.
FT   MUTAGEN     684    684       H->R: Only 23% zinc-induced beta-APP (28)
FT                                peptide aggregation.
FT   MUTAGEN     704    704       G->V: Reduced protein oxidation. No
FT                                hippocampal neuron toxicity.
FT   MUTAGEN     706    706       M->L: Reduced lipid peroxidation
FT                                inhibition.
FT   MUTAGEN     706    706       M->V: No free radical production. No
FT                                hippocampal neuron toxicity.
FT   MUTAGEN     717    717       V->C,S: Unchanged beta-APP42/total APP-
FT                                beta ratio.
FT   MUTAGEN     717    717       V->F,G,I: Increased beta-APP42/beta-APP40
FT                                ratio.
FT   MUTAGEN     717    717       V->K: Decreased beta-APP42/total APP-beta
FT                                ratio.
FT   MUTAGEN     717    717       V->M: Increased beta-APP42/beta-APP40
FT                                ratio. No change in apoptosis after
FT                                caspase cleavage.
FT   MUTAGEN     728    728       Y->A: No effect on APBA1 nor APBB1
FT                                binding. Greatly reduces the binding to
FT                                APPBP2. APP internalization unchanged. No
FT                                change in beta-APP42 secretion.
FT   MUTAGEN     739    739       D->A: No cleavage by caspases during
FT                                apoptosis.
FT   MUTAGEN     739    739       D->N: No effect on FADD-induced
FT                                apoptosis.
FT   MUTAGEN     743    743       T->A: Greatly reduces the binding to SHC1
FT                                and APBB family members; no effect on
FT                                NGF-stimulated neurite extension.
FT   MUTAGEN     743    743       T->E: Reduced NGF-stimulated neurite
FT                                extension. No effect on APP maturation.
FT   MUTAGEN     756    756       G->A: APP internalization unchanged. No
FT                                change in beta-APP42 secretion.
FT   MUTAGEN     757    757       Y->A: Little APP internalization. Reduced
FT                                beta-APP42 secretion.
FT   MUTAGEN     757    757       Y->G: Loss of binding to MAPK8IP1, APBA1,
FT                                APBB1, APPBP2 and SHC1.
FT   MUTAGEN     759    759       N->A: No binding to APBA1, no effect on
FT                                APBB1 binding. Little APP
FT                                internalization. Reduced beta-APP42
FT                                secretion.
FT   MUTAGEN     760    760       P->A: Little APP internalization. Reduced
FT                                beta-APP42 secretion.
FT   MUTAGEN     762    762       Y->A: Loss of binding to APBA1 and APBB1.
FT                                APP internalization unchanged. No change
FT                                in beta-APP42 secretion.
FT   CONFLICT     15     16       AR -> VW (in Ref. 3; CAA31830).
FT   CONFLICT    647    647       D -> E (in Ref. 36; AAA51722).
FT   CONFLICT    724    724       Missing (in Ref. 23; AAB26263/AAB26264).
FT   CONFLICT    731    731       I -> N (in Ref. 23; AAB26263/AAB26264/
FT                                AAB26265).
FT   CONFLICT    757    757       Y -> S (in Ref. 31; AAA35540).
FT   STRAND       33     35
FT   STRAND       43     45
FT   TURN         47     49
FT   STRAND       52     54
FT   HELIX        66     76
FT   STRAND       82     87
FT   STRAND       92     94
FT   STRAND       97     99
FT   HELIX       100    102
FT   STRAND      103    106
FT   STRAND      110    112
FT   STRAND      115    119
FT   STRAND      134    139
FT   HELIX       147    160
FT   STRAND      163    174
FT   TURN        175    177
FT   STRAND      178    188
FT   HELIX       288    292
FT   STRAND      299    301
FT   STRAND      304    310
FT   TURN        311    314
FT   STRAND      315    321
FT   STRAND      323    325
FT   STRAND      331    333
FT   HELIX       334    341
FT   HELIX       374    380
FT   HELIX       389    418
FT   STRAND      421    423
FT   HELIX       425    480
FT   STRAND      482    484
FT   HELIX       487    518
FT   HELIX       520    546
FT   HELIX       547    550
FT   HELIX       552    566
FT   HELIX       673    675
FT   STRAND      679    682
FT   STRAND      683    685
FT   STRAND      688    691
FT   HELIX       695    697
FT   STRAND      698    700
FT   STRAND      702    705
FT   STRAND      707    712
FT   HELIX       744    754
FT   STRAND      755    758
FT   STRAND      763    765
SQ   SEQUENCE   770 AA;  86943 MW;  A12EE761403740F5 CRC64;
     MLPGLALLLL AAWTARALEV PTDGNAGLLA EPQIAMFCGR LNMHMNVQNG KWDSDPSGTK
     TCIDTKEGIL QYCQEVYPEL QITNVVEANQ PVTIQNWCKR GRKQCKTHPH FVIPYRCLVG
     EFVSDALLVP DKCKFLHQER MDVCETHLHW HTVAKETCSE KSTNLHDYGM LLPCGIDKFR
     GVEFVCCPLA EESDNVDSAD AEEDDSDVWW GGADTDYADG SEDKVVEVAE EEEVAEVEEE
     EADDDEDDED GDEVEEEAEE PYEEATERTT SIATTTTTTT ESVEEVVREV CSEQAETGPC
     RAMISRWYFD VTEGKCAPFF YGGCGGNRNN FDTEEYCMAV CGSAMSQSLL KTTQEPLARD
     PVKLPTTAAS TPDAVDKYLE TPGDENEHAH FQKAKERLEA KHRERMSQVM REWEEAERQA
     KNLPKADKKA VIQHFQEKVE SLEQEAANER QQLVETHMAR VEAMLNDRRR LALENYITAL
     QAVPPRPRHV FNMLKKYVRA EQKDRQHTLK HFEHVRMVDP KKAAQIRSQV MTHLRVIYER
     MNQSLSLLYN VPAVAEEIQD EVDELLQKEQ NYSDDVLANM ISEPRISYGN DALMPSLTET
     KTTVELLPVN GEFSLDDLQP WHSFGADSVP ANTENEVEPV DARPAADRGL TTRPGSGLTN
     IKTEEISEVK MDAEFRHDSG YEVHHQKLVF FAEDVGSNKG AIIGLMVGGV VIATVIVITL
     VMLKKKQYTS IHHGVVEVDA AVTPEERHLS KMQQNGYENP TYKFFEQMQN
Mascot: http://www.matrixscience.com/