Parameter Value Version 1.5.9.3 User name maxquant Machine name PCMANN174 Date of writing 06/07/2017 13:38:55 Fixed modifications Carbamidomethyl (C) Include contaminants True PSM FDR 0.01 Protein FDR 0.01 Site FDR 0.01 Use Normalized Ratios For Occupancy True Min. peptide Length 7 Min. score for unmodified peptides 0 Min. score for modified peptides 40 Min. delta score for unmodified peptides 0 Min. delta score for modified peptides 6 Min. unique peptides 0 Min. razor peptides 1 Min. peptides 1 Use only unmodified peptides and True Modifications included in protein quantification Oxidation (M);Acetyl (Protein N-term) Peptides used for protein quantification Razor Discard unmodified counterpart peptides True Label min. ratio count 2 Use delta score False iBAQ False iBAQ log fit False Match between runs True Matching time window [min] 0.7 Alignment time window [min] 20 Find dependent peptides False Fasta file E:\fasta\2015\UP000005640_9606.fasta;E:\fasta\2015\UP000005640_9606_additional.fasta Decoy mode revert Include contaminants True Advanced ratios True Fixed andromeda index folder Temporary folder Combined folder location Second peptides True Stabilize large LFQ ratios True Separate LFQ in parameter groups False Require MS/MS for LFQ comparisons True Calculate peak properties False Main search max. combinations 200 Advanced site intensities True Write msScans table True Write msmsScans table True Write ms3Scans table True Write allPeptides table True Write mzRange table True Max. peptide mass [Da] 4600 Min. peptide length for unspecific search 8 Max. peptide length for unspecific search 25 Razor protein FDR True Disable MD5 False Max mods in site table 3 Match unidentified features False Taxonomy level Species Top 3 False Match between runs FDR False RT shift False AIF correlation 0 First pass AIF correlation 0 AIF topx 0 AIF min mass 0 AIF SIL weight 0 AIF ISO weight 0 AIF iterative False AIF threshold FDR 0 AIF MS/MS tolerance [ppm] 0 AIF Second pass False Epsilon score for mutations Evaluate variant peptides separately True Evaluate proteogenomic peptides separately True AVALON False De-novo sequencing False De-novo include variable modifications True Mass difference search False Mass difference search modifications System.String[] Variation mode None Variation parse rule Custom protein quantification False Custom protein quantification file Split protein groups by taxonomy ID False Show centroid mass differences False Show isotope mass differences False Connect MS/MS False Composition prediction None Verbose table column headers False MS/MS tol. (FTMS) 20 ppm Top MS/MS peaks per Da interval. (FTMS) 12 Da interval. (FTMS) 100 MS/MS deisotoping (FTMS) True MS/MS deisotoping tolerance (FTMS) 7 MS/MS deisotoping tolerance unit (FTMS) ppm MS/MS higher charges (FTMS) True MS/MS water loss (FTMS) True MS/MS ammonia loss (FTMS) True MS/MS dependent losses (FTMS) True MS/MS recalibration (FTMS) False MS/MS de novo tolerance (FTMS) 10 MS/MS de novo tolerance unit (FTMS) ppm MS/MS tol. (ITMS) 0.5 Da Top MS/MS peaks per Da interval. (ITMS) 8 Da interval. (ITMS) 100 MS/MS deisotoping (ITMS) False MS/MS deisotoping tolerance (ITMS) 0.15 MS/MS deisotoping tolerance unit (ITMS) Da MS/MS higher charges (ITMS) True MS/MS water loss (ITMS) True MS/MS ammonia loss (ITMS) True MS/MS dependent losses (ITMS) True MS/MS recalibration (ITMS) False MS/MS de novo tolerance (ITMS) 0.25 MS/MS de novo tolerance unit (ITMS) Da MS/MS tol. (TOF) 40 ppm Top MS/MS peaks per Da interval. (TOF) 10 Da interval. (TOF) 100 MS/MS deisotoping (TOF) True MS/MS deisotoping tolerance (TOF) 0.01 MS/MS deisotoping tolerance unit (TOF) Da MS/MS higher charges (TOF) True MS/MS water loss (TOF) True MS/MS ammonia loss (TOF) True MS/MS dependent losses (TOF) True MS/MS recalibration (TOF) False MS/MS de novo tolerance (TOF) 0.02 MS/MS de novo tolerance unit (TOF) Da MS/MS tol. (Unknown) 0.5 Da Top MS/MS peaks per Da interval. (Unknown) 8 Da interval. (Unknown) 100 MS/MS deisotoping (Unknown) False MS/MS deisotoping tolerance (Unknown) 0.15 MS/MS deisotoping tolerance unit (Unknown) Da MS/MS higher charges (Unknown) True MS/MS water loss (Unknown) True MS/MS ammonia loss (Unknown) True MS/MS dependent losses (Unknown) True MS/MS recalibration (Unknown) False MS/MS de novo tolerance (Unknown) 0.25 MS/MS de novo tolerance unit (Unknown) Da Site tables Oxidation (M)Sites.txt