Recombinant Arabidopsis phototropin2 (Phot2) protein or kinase-dead derivative (Phot2-DN) were incubated with ATP under different conditions. Proteins were separated by SDS-PAGE, in-gel tryptic digested, analyzed by LC-MS. 2018-10-09 Hirofumi Nakagami Max Planck Institute for Plant Breeding Research Hirofumi Nakagami Arabidopsis, phototropin2, phosphorylation 29196607 Oide M, Okajima K, Nakagami H, Kato T, Sekiguchi Y, Oroguchi T, Hikima T, Yamamoto M, Nakasako M Blue light-excited LOV1 and LOV2 domains cooperatively regulate the kinase activity of full-length phototropin2 from <i>Arabidopsis</i>. J Biol Chem. 2018 Jan 19;293(3):963-972 Phot2_WT.raw raw 46359088 Arabidopsis thaliana (Mouse-ear cress) Tissue not applicable to dataset Trypsin Carboxymethyl (C) Phospho (S) Arabidopsis thaliana (Mouse-ear cress) Phospho (T) Phospho (Y) Oxidation (M) LTQ Orbitrap DDA-high res. Qualification - Phot2_DN.raw raw 46454812 Arabidopsis thaliana (Mouse-ear cress) Tissue not applicable to dataset Trypsin Carboxymethyl (C) Phospho (S) Arabidopsis thaliana (Mouse-ear cress) Phospho (T) Phospho (Y) Oxidation (M) LTQ Orbitrap DDA-high res. Qualification - AtPHOT2_PP.raw raw 43757242 Arabidopsis thaliana (Mouse-ear cress) Tissue not applicable to dataset Trypsin Carboxymethyl (C) Phospho (S) Arabidopsis thaliana (Mouse-ear cress) Phospho (T) Phospho (Y) Oxidation (M) LTQ Orbitrap DDA-high res. Qualification - AtPHOT2_Dark_1h.raw raw 43839920 Arabidopsis thaliana (Mouse-ear cress) Tissue not applicable to dataset Trypsin Carboxymethyl (C) Phospho (S) Arabidopsis thaliana (Mouse-ear cress) Phospho (T) Phospho (Y) Oxidation (M) LTQ Orbitrap DDA-high res. Qualification - AtPHOT2_BL_1h.raw raw 43731160 Arabidopsis thaliana (Mouse-ear cress) Tissue not applicable to dataset Trypsin Carboxymethyl (C) Phospho (S) Arabidopsis thaliana (Mouse-ear cress) Phospho (T) Phospho (Y) Oxidation (M) LTQ Orbitrap DDA-high res. Qualification - F022140.dat result 13140636 Mascot F022138.dat result 13679564 Mascot