*******************************************************************************
--------------------------------- IADBs ---------------------------------------
--------------------- Copyright © 2013 Waters Corporation ---------------------
Version 2.135.2.0 Compiled: 10/31/2013 1:59 PM
Run at: 4/14/2018 8:02 PM
Command Line:

-paraXMLFileName C:\Users\waters\AppData\Local\Temp\plgs7720930122632459699.params -pep3DFilename C:\Users\waters\AppData\Local\Temp\plgs7720930122632459699.bin -proteinFASTAFileName C:\PLGS3.0.2\demodbs\uniprot-reviewed_viridiplantae.fasta_def -outPutDirName C:\Users\waters\AppData\Local\Temp -outputUserDirName C:\Users\waters\AppData\Local\Temp -newWorkflowXML -maxCPUs 11

 Parameter XML file:
<?xml version="1.0" encoding="UTF-8"?>
<IA_PARAMS>
    <FASTA_FORMAT VALUE="DEF"/>
    <PRECURSOR_MHP_WINDOW_PPM VALUE="50"/>
    <PRODUCT_MHP_WINDOW_PPM VALUE="100"/>
    <NUM_BY_MATCH_FOR_PEPTIDE_MINIMUM VALUE="2"/>
    <NUM_PEPTIDE_FOR_PROTEIN_MINIMUM VALUE="1"/>
    <NUM_BY_MATCH_FOR_PROTEIN_MINIMUM VALUE="5"/>
    <PROTEIN_MASS_MAXIMUM_AMU VALUE="250000"/>
    <FALSE_POSITIVE_RATE VALUE="100"/>
    <AQ_PROTEIN_ACCESSION VALUE=""/>
    <AQ_PROTEIN_MOLES VALUE="-1"/>
    <MANUAL_RESPONSE_FACTOR VALUE="-1"/>
    <DIGESTS>
        <ANALYSIS_DIGESTOR MISSED_CLEAVAGES="1">
            <AMINO_ACID_SEQUENCE_DIGESTOR NAME="Trypsin" UUID="0a017806-3674-44be-affa-1413ee6f81fb">
                <CLEAVES_AT AMINO_ACID="K" POSITION="C-TERM">
                    <EXCLUDES AMINO_ACID="P" POSITION="N-TERM"/>
                </CLEAVES_AT>
                <CLEAVES_AT AMINO_ACID="R" POSITION="C-TERM">
                    <EXCLUDES AMINO_ACID="P" POSITION="N-TERM"/>
                </CLEAVES_AT>
            </AMINO_ACID_SEQUENCE_DIGESTOR>
        </ANALYSIS_DIGESTOR>
    </DIGESTS>
    <MODIFICATIONS>
        <ANALYSIS_MODIFIER ENRICHED="FALSE" STATUS="FIXED">
            <MODIFIER MCAT_REAGENT="No" NAME="Carbamidomethyl+C">
                <MODIFIES APPLIES_TO="C" DELTA_MASS="57.0215" TYPE="SIDECHAIN"/>
            </MODIFIER>
        </ANALYSIS_MODIFIER>
        <ANALYSIS_MODIFIER ENRICHED="FALSE" STATUS="VARIABLE">
            <MODIFIER MCAT_REAGENT="No" NAME="Oxidation+M">
                <MODIFIES APPLIES_TO="M" DELTA_MASS="15.9949" TYPE="SIDECHAIN"/>
            </MODIFIER>
        </ANALYSIS_MODIFIER>
    </MODIFICATIONS>
</IA_PARAMS>


  Input parameters                                values
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 Parameter XML file:       "C:\Users\waters\AppData\Local\Temp\plgs7720930122632459699.params"
 Spectrum  Bin File:       "C:\Users\waters\AppData\Local\Temp\plgs7720930122632459699.bin"
 Protein   FAS File:       "C:\PLGS3.0.2\demodbs\uniprot-reviewed_viridiplantae.fasta_def"
 Output Directory:         "C:\Users\waters\AppData\Local\Temp"
 User Output Directory:    "C:\Users\waters\AppData\Local\Temp"

 Instrument Model:   SYNAPT-G2
 Instrument Serial#: NotSet
 Raw Input File: "D:\Data\2017\December\OU_Bindhu\02122017_TL_OU_Bindhu_3.raw"
 Acquired Name: "02122017_TL_OU_Bindhu_3"
 Acquired Date: "30-Dec-1899"
 Acquired Time: "00:00:00"
 Sample Description: "02122017_TL_OU_Bindhu_3"

Processed by:
 Apex3D version 2.128.9.0,  compiled: 9/19/2013 processed: 4/14/2018
    command line: C:\PLGS3.0.2\bin\..\lib\apex3d\Apex3D64.exe -pRawDirName D:\Data\2017\December\OU_Bindhu\02122017_TL_OU_Bindhu_3.raw -outputDirName C:\Users\waters\AppData\Local\Temp -outputUserDirName C:\Users\waters\AppData\Local\Temp -lockmassZ2 785.8426 -lockMassToleranceAMU 0.5 -leThresholdCounts 135.0 -heThresholdCounts 20.0 -binIntenThreshold 1000 -writeXML 1 -PLGS 1 

 Peptide3D version 2.96.5010.13362,  compiled: 9/19/2013 processed: 4/14/2018
    command line: C:\PLGS3.0.2\lib\apex3d\Peptide3D.exe -inputFilename C:\Users\waters\AppData\Local\Temp\02122017_tl_ou_bindhu_3_Apex3D.bin -outputDirName C:\Users\waters\AppData\Local\Temp -outputUserDirName C:\Users\waters\AppData\Local\Temp -minLEMHPlus 350.0


Resolution                  = 13461.13
Precursor MHPlus Window PPM = 50 (set by user)
Product MHPlus Window PPM   = 100 (set by user)
Max MHPlus Window PPM       = 200
Bin FWHM Fraction           = 0.1428571
Report homologs             = True
Min fragments per peptide   = 2
Min peptides per protein    = 1
Min fragments per protein   = 5
Max False positive rate     = 100
Pass 1 False positive rate  = 100
Max protein mass            = 250000
Min ratio by to theoretical = 0.3
Min sumProdInten/precInten  = 0.03
Max sumProdInten/precInten  = 1
Max simultaneous varMods    = 3
Max CPUs                    = 11
ScoreFactors                = 1, 9, 3.5, 1, 1.9, 1, 2, 1
No Pass 2                   = False
No Pass 3                   = False
Non-specific Pass           = False

Read 124115 LE ions, 1547062 HE ions (1547062 binned), 70379 bins in 30.2257474 seconds
Read 78822 proteins in 3.9936689 seconds
Matched 1202085 out of 2186819 peptides in digest to 1202085 matches in 75944  of 78822 proteins
Insufficient pass 0 matches for mass cal
Matched 1202085 out of 2186819 peptides in digest to 1202085 matches in 75944  of 78822 proteins
After Pass1:  2943 distraction, 5490 regular proteins
Time to calc all pass2 raw pepScores 00:00:03.9786359

Calibrating AutoCurate Thresholds using 1747605 distraction peptides
Median Random pepScore 5.724366 Std Random pepScore 0.3069844
Red-Yellow Threshold is 6.49 which is 2.50 stddevs above the mean
Yellow-Green Threshold is 6.95 which is 4.00 stddevs above the mean
Median Random raw pepScore 284.8236 Std Random raw pepScore 259.9276
Time to run pass 2 depletion: 00:05:50.2273989
After Pass2 Depletion:  1897 distraction, 4319 regular proteins
After Filter Proteins:  0 distraction, 4319 regular proteins
Total processing time: 1096.0965036 seconds