Mascot Search Results

Match to: TRI46_MOUSE Score: 916
(Q7TNM2) Tripartite motif protein 46 (Tripartite, fibronectin type-III and C-terminal SPRY motif protein)
Found in search of G:\TN9\TN9.mgf

Nominal mass (Mr): 83378; Calculated pI value: 7.85
NCBI BLAST search of TRI46_MOUSE against nr
Unformatted sequence string for pasting into other applications

Taxonomy: Mus musculus

Variable modifications: Carboxymethyl (C),Oxidation (M),Phospho (ST)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Sequence Coverage: 6%

Matched peptides shown in Bold Red

     1 MAEGEDMQTF TSIMDALVRI STSMKNMEKE LLCPVCQEMY KQPLVLPCTH 
    51 NVCQACAREV LGQQGYIGHG GDPSSEPTSP ASTPSTRSPR LSRRTLPKPD 
   101 RLDRLLKSGF GTYPGRKRGA LHPQTILFPC PACQGDVELG ERGLSGLFRN 
   151 LTLERVVERY RQSVSVGGAI LCQLCKPPPL EATKGCTECR ATFCNECFKL 
   201 FHPWGTQKAQ HEPTLPTLSF RPKGLMCPDH KEEVTHYCKT CQRLVCQLCR 
   251 VRRTHSGHKI TPVLSAYQAL KDKLTKSLAY ILGNQDTVQT QICELEETIR 
   301 HTEVSGQQAK EEVSQLVRGL GAVLEEKRAS LLQAIEECQQ ERLSRLSAQI 
   351 HEHQSLLDGS GLVGYAQEVL KETDQPCFVQ AAKQLHNRIA RATEALQTFR 
   401 PAASSSFRHC QLDVGREMKL LTELSFLRVP EAPVIDTQRT FAYDQIFLCW 
   451 RLPPHSPPAW HYTVEFRRTD VPAQPGPTRW QRREEVRGTS ALLENPDTGS 
   501 VYVLRVRGCN KAGYGEYSED VHLHTPPAPV LHFFLDGRWG ASRERLAISK 
   551 DQRAVRSIPG LPLLLAAERL LTGCHLSVDV VLGDVAVTQG RSYWACAVDP 
   601 ASYLVKVGVG LESKLQESFQ GAPDVISPRY DPDSGHDSGA EDAAVEALPP 
   651 FAFLTIGMGK ILLGSGASSN AGLTGRDGPT ASCTVPLPPR LGICLDYERG 
   701 RVSFLDAVSF RGLLECPLDC SGPVCPAFCF IGGGAVQLQE PVGTKPERKV 
   751 TIGGFAKLD

  Residue Number  Increasing Mass  Decreasing Mass 

 Start - End     Observed    Mr(expt)   Mr(calc)     Delta   Miss Sequence
    30 - 41      727.3386  1452.6627  1452.6451     0.0176     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Glu->pyro-Glu (N-term E) [-18.01] (Ions score 31)
    30 - 41      727.8427  1453.6707  1453.7131    -0.0424     0  K.ELLCPVCQEMYK.Q  Glu->Lys (E) [-0.95] (Ions score 20)
    30 - 41      728.3412  1454.6679  1454.6244     0.0436     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 28)
    30 - 41      728.3452  1454.6759  1454.6244     0.0515     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 44)
    30 - 41      728.3458  1454.6771  1454.6244     0.0527     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 59)
    30 - 41      728.3463  1454.6781  1454.6244     0.0537     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 64)
    30 - 41      728.3469  1454.6793  1454.6244     0.0549     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 61)
    30 - 41      728.3476  1454.6806  1454.6244     0.0563     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 36)
    30 - 41      728.3478  1454.6810  1454.6244     0.0566     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 56)
    30 - 41      728.3491  1454.6836  1454.6244     0.0592     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 62)
    30 - 41      728.3498  1454.6851  1454.6244     0.0608     0  K.ELLCPVCQEMYK.Q  Lys->Gln (K) [-0.04] (Ions score 48)
    30 - 41      728.3457  1454.6768  1454.6971    -0.0203     0  K.ELLCPVCQEMYK.Q  Gln->Lys (Q) [+0.04] (Ions score 64)
    30 - 41      728.8474  1455.6801  1455.6448     0.0354     0  K.ELLCPVCQEMYK.Q  Deamidated (NQ) [+0.98] (Ions score 33)
    30 - 41      728.8476  1455.6806  1455.6448     0.0359     0  K.ELLCPVCQEMYK.Q  Deamidated (NQ) [+0.98] (Ions score 43)
    30 - 41      735.3379  1468.6612  1468.6764    -0.0152     0  K.ELLCPVCQEMYK.Q  Methyl (DE) [+14.02] (Ions score 20)
    30 - 41      736.3430  1470.6714  1470.6193     0.0521     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 40)
    30 - 41      736.3433  1470.6721  1470.6193     0.0528     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 48)
    30 - 41      736.3440  1470.6734  1470.6193     0.0541     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 41)
    30 - 41      736.3441  1470.6735  1470.6193     0.0543     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 44)
    30 - 41      736.3449  1470.6753  1470.6193     0.0560     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 40)
    30 - 41      736.3425  1470.6704  1470.6557     0.0147     0  K.ELLCPVCQEMYK.Q  Oxidation (C) [+15.99] (Ions score 31)
    30 - 41      736.3434  1470.6722  1470.6557     0.0165     0  K.ELLCPVCQEMYK.Q  Oxidation (C) [+15.99] (Ions score 50)
    30 - 41      743.3267  1484.6389  1484.6349     0.0040     0  K.ELLCPVCQEMYK.Q  Val->Glu (V) [+29.97] (Ions score 31)
    30 - 41      744.3413  1486.6681  1486.6328     0.0352     0  K.ELLCPVCQEMYK.Q  Sulfide (C) [+31.97] (Ions score 32)
    30 - 41      749.8487  1497.6828  1497.6666     0.0163     0  K.ELLCPVCQEMYK.Q  Carbamyl (C) [+43.01] (Ions score 52)
    30 - 41      749.8491  1497.6836  1497.6666     0.0170     0  K.ELLCPVCQEMYK.Q  Carbamyl (C) [+43.01] (Ions score 54)
    30 - 41      752.3413  1502.6681  1502.6455     0.0226     0  K.ELLCPVCQEMYK.Q  Trioxidation (C) [+47.98] (Ions score 62)
    30 - 41      757.8477  1513.6809  1513.6615     0.0194     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Carbamyl (C) [+43.01] (Ions score 49)
    30 - 41      760.3350  1518.6554  1518.6404     0.0150     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Trioxidation (C) [+47.98] (Ions score 49)
    30 - 41      760.3359  1518.6573  1518.6404     0.0169     0  K.ELLCPVCQEMYK.Q  Oxidation (M); Trioxidation (C) [+47.98] (Ions score 44)
    30 - 41      788.3387  1574.6628  1574.6853    -0.0224     0  K.ELLCPVCQEMYK.Q  DTT_C (C) [+120.02] (Ions score 21)
    30 - 41      788.3391  1574.6635  1574.6853    -0.0217     0  K.ELLCPVCQEMYK.Q  DTT_C (C) [+120.02] (Ions score 26)
    30 - 41      789.3483  1576.6821  1576.6855    -0.0034     0  K.ELLCPVCQEMYK.Q  2 Carboxymethyl (C); Label:13C(5)15N(1) (V) [+6.01] (Ions score 23)
    30 - 41      789.3492  1576.6839  1576.6855    -0.0016     0  K.ELLCPVCQEMYK.Q  2 Carboxymethyl (C); Label:13C(5)15N(1) (V) [+6.01] (Ions score 32)
    30 - 41      797.3444  1592.6742  1592.6804    -0.0063     0  K.ELLCPVCQEMYK.Q  2 Carboxymethyl (C); Oxidation (M); Label:13C(5)15N(1) (V) [+6.01] (Ions score 22)
    30 - 41      862.3345  1722.6545  1722.8646    -0.2101     0  K.ELLCPVCQEMYK.Q  Carboxymethyl (C); Myristoyl (C) [+210.20] (Ions score 25)
   108 - 117     357.1857  1068.5354  1068.4989     0.0365     1  K.SGFGTYPGRK.R  Lys->Gln (K) [-0.04] (Ions score 24)
   108 - 117     535.2751  1068.5356  1068.4989     0.0367     1  K.SGFGTYPGRK.R  Lys->Gln (K) [-0.04] (Ions score 39)
   108 - 117     357.1859  1068.5358  1068.4989     0.0369     1  K.SGFGTYPGRK.R  Lys->Gln (K) [-0.04] (Ions score 18)
   108 - 117     535.2752  1068.5359  1068.4989     0.0370     1  K.SGFGTYPGRK.R  Lys->Gln (K) [-0.04] (Ions score 29)
   108 - 117     575.2581  1148.5017  1148.4652     0.0365     1  K.SGFGTYPGRK.R  Phospho (ST); Lys->Gln (K) [-0.04] (Ions score 37)
   108 - 117     575.2581  1148.5017  1148.4652     0.0365     1  K.SGFGTYPGRK.R  Phospho (ST); Lys->Gln (K) [-0.04] (Ions score 25)
   108 - 117     383.8413  1148.5019  1148.4652     0.0367     1  K.SGFGTYPGRK.R  Phospho (ST); Lys->Gln (K) [-0.04] (Ions score 26)
   108 - 117     409.2196  1224.6371  1224.6503    -0.0132     1  K.SGFGTYPGRK.R  HNE (CHK) [+156.12] (Ions score 27)
   118 - 142     902.4590  2704.3551  2704.3061     0.0490     1  K.RGALHPQTILFPCPACQGDVELGER.G  Carboxymethyl (C); Phe->Ser (F) [-60.04] (Ions score 30)
   118 - 142     921.8012  2762.3816  2762.3996    -0.0180     1  K.RGALHPQTILFPCPACQGDVELGER.G  2 Ethyl (E) [+28.03] (Ions score 35)


ID   TRI46_MOUSE    STANDARD;      PRT;   759 AA.
AC   Q7TNM2; Q60717; Q6P1I9;
DT   01-FEB-2005 (Rel. 46, Created)
DT   01-FEB-2005 (Rel. 46, Last sequence update)
DT   10-MAY-2005 (Rel. 47, Last annotation update)
DE   Tripartite motif protein 46 (Tripartite, fibronectin type-III and C-
DE   terminal SPRY motif protein).
GN   Name=Trim46; Synonyms=Trific;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE (ISOFORM 1).
RC   STRAIN=C57BL/6;
RA   Short K.M., Cox T.C.;
RT   "The COS box: a novel coiled-coil associated signature for a new
RT   subgroup of RBCC proteins.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6; TISSUE=Brain;
RX   MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA   Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA   Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA   Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA   Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA   Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA   Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA   Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA   Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA   Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA   Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA   Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA   Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA   Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA   Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA   Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human
RT   and mouse cDNA sequences.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE OF 630-759 (ISOFORM 1).
RC   STRAIN=BALB/c;
RX   MEDLINE=96169575; PubMed=8597643;
RA   Vos H.L., Mockensturm-Wilson M., Rood P.M., Maas A.M., Duhig T.,
RA   Gendler S.J., Bornstein P.;
RT   "A tightly organized, conserved gene cluster on mouse chromosome 3
RT   (E3-F1).";
RL   Mamm. Genome 6:820-822(1995).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q7TNM2-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7TNM2-2; Sequence=VSP_011985, VSP_011986, VSP_011987;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the TRIM/RBCC family.
CC   -!- SIMILARITY: Contains 1 B box-type zinc finger.
CC   -!- SIMILARITY: Contains 1 B30.2-like domain.
CC   -!- SIMILARITY: Contains 1 fibronectin type-III domain.
CC   -!- SIMILARITY: Contains 2 RING-type zinc fingers.
CC   --------------------------------------------------------------------------
CC   This Swiss-Prot entry is copyright. It is produced through a collaboration
CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
CC   use as long as its content is in no way modified and this statement is not
CC   removed.
CC   --------------------------------------------------------------------------
DR   EMBL; AY251388; AAP51208.1; -; mRNA.
DR   EMBL; BC065049; AAH65049.1; ALT_INIT; mRNA.
DR   EMBL; U16175; AAA98539.1; -; Genomic_DNA.
DR   Ensembl; ENSMUSG00000042766; Mus musculus.
DR   MGI; MGI:2673000; Trim46.
DR   InterPro; IPR001870; B302.
DR   InterPro; IPR003961; FN_III.
DR   InterPro; IPR000315; Znf_Bbox.
DR   InterPro; IPR001841; Znf_ring.
DR   Pfam; PF00041; fn3; 1.
DR   Pfam; PF00643; zf-B_box; 1.
DR   Pfam; PF00097; zf-C3HC4; 1.
DR   SMART; SM00336; BBOX; 1.
DR   SMART; SM00060; FN3; 1.
DR   SMART; SM00184; RING; 1.
DR   PROSITE; PS50853; FN3; 1.
DR   PROSITE; PS50119; ZF_BBOX; 1.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; FALSE_NEG.
KW   Alternative splicing; Coiled coil; Metal-binding; Zinc; Zinc-finger.
FT   DOMAIN      430    525       Fibronectin type-III.
FT   DOMAIN      539    728       B30.2-like.
FT   ZN_FING      33     73       RING-type 1 (degenerate).
FT   ZN_FING     172    231       RING-type 2 (degenerate).
FT   ZN_FING     222    263       B box-type.
FT   COILED      322    400       Potential.
FT   VARSPLIC      1     23       MAEGEDMQTFTSIMDALVRISTS -> MGGALEINAINPEM
FT                                EGWRQTS (in isoform 2).
FT                                /FTId=VSP_011985.
FT   VARSPLIC    429    447       VPEAPVIDTQRTFAYDQIF -> GCGHRGLCSGAPQCPRRP
FT                                S (in isoform 2).
FT                                /FTId=VSP_011986.
FT   VARSPLIC    448    759       Missing (in isoform 2).
FT                                /FTId=VSP_011987.
FT   CONFLICT    743    743       G -> A (in Ref. 3).
SQ   SEQUENCE   759 AA;  83432 MW;  8126C4C79841A536 CRC64;
     MAEGEDMQTF TSIMDALVRI STSMKNMEKE LLCPVCQEMY KQPLVLPCTH NVCQACAREV
     LGQQGYIGHG GDPSSEPTSP ASTPSTRSPR LSRRTLPKPD RLDRLLKSGF GTYPGRKRGA
     LHPQTILFPC PACQGDVELG ERGLSGLFRN LTLERVVERY RQSVSVGGAI LCQLCKPPPL
     EATKGCTECR ATFCNECFKL FHPWGTQKAQ HEPTLPTLSF RPKGLMCPDH KEEVTHYCKT
     CQRLVCQLCR VRRTHSGHKI TPVLSAYQAL KDKLTKSLAY ILGNQDTVQT QICELEETIR
     HTEVSGQQAK EEVSQLVRGL GAVLEEKRAS LLQAIEECQQ ERLSRLSAQI HEHQSLLDGS
     GLVGYAQEVL KETDQPCFVQ AAKQLHNRIA RATEALQTFR PAASSSFRHC QLDVGREMKL
     LTELSFLRVP EAPVIDTQRT FAYDQIFLCW RLPPHSPPAW HYTVEFRRTD VPAQPGPTRW
     QRREEVRGTS ALLENPDTGS VYVLRVRGCN KAGYGEYSED VHLHTPPAPV LHFFLDGRWG
     ASRERLAISK DQRAVRSIPG LPLLLAAERL LTGCHLSVDV VLGDVAVTQG RSYWACAVDP
     ASYLVKVGVG LESKLQESFQ GAPDVISPRY DPDSGHDSGA EDAAVEALPP FAFLTIGMGK
     ILLGSGASSN AGLTGRDGPT ASCTVPLPPR LGICLDYERG RVSFLDAVSF RGLLECPLDC
     SGPVCPAFCF IGGGAVQLQE PVGTKPERKV TIGGFAKLD