Match to: TRI46_MOUSE Score: 916 (Q7TNM2) Tripartite motif protein 46 (Tripartite, fibronectin type-III and C-terminal SPRY motif protein) Found in search of G:\TN9\TN9.mgf Nominal mass (Mr): 83378; Calculated pI value: 7.85 NCBI BLAST search of TRI46_MOUSE against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Carboxymethyl (C),Oxidation (M),Phospho (ST) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 6% Matched peptides shown in Bold Red 1 MAEGEDMQTF TSIMDALVRI STSMKNMEKE LLCPVCQEMY KQPLVLPCTH 51 NVCQACAREV LGQQGYIGHG GDPSSEPTSP ASTPSTRSPR LSRRTLPKPD 101 RLDRLLKSGF GTYPGRKRGA LHPQTILFPC PACQGDVELG ERGLSGLFRN 151 LTLERVVERY RQSVSVGGAI LCQLCKPPPL EATKGCTECR ATFCNECFKL 201 FHPWGTQKAQ HEPTLPTLSF RPKGLMCPDH KEEVTHYCKT CQRLVCQLCR 251 VRRTHSGHKI TPVLSAYQAL KDKLTKSLAY ILGNQDTVQT QICELEETIR 301 HTEVSGQQAK EEVSQLVRGL GAVLEEKRAS LLQAIEECQQ ERLSRLSAQI 351 HEHQSLLDGS GLVGYAQEVL KETDQPCFVQ AAKQLHNRIA RATEALQTFR 401 PAASSSFRHC QLDVGREMKL LTELSFLRVP EAPVIDTQRT FAYDQIFLCW 451 RLPPHSPPAW HYTVEFRRTD VPAQPGPTRW QRREEVRGTS ALLENPDTGS 501 VYVLRVRGCN KAGYGEYSED VHLHTPPAPV LHFFLDGRWG ASRERLAISK 551 DQRAVRSIPG LPLLLAAERL LTGCHLSVDV VLGDVAVTQG RSYWACAVDP 601 ASYLVKVGVG LESKLQESFQ GAPDVISPRY DPDSGHDSGA EDAAVEALPP 651 FAFLTIGMGK ILLGSGASSN AGLTGRDGPT ASCTVPLPPR LGICLDYERG 701 RVSFLDAVSF RGLLECPLDC SGPVCPAFCF IGGGAVQLQE PVGTKPERKV 751 TIGGFAKLD
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 30 - 41 727.3386 1452.6627 1452.6451 0.0176 0 K.ELLCPVCQEMYK.Q Oxidation (M); Glu->pyro-Glu (N-term E) [-18.01] (Ions score 31) 30 - 41 727.8427 1453.6707 1453.7131 -0.0424 0 K.ELLCPVCQEMYK.Q Glu->Lys (E) [-0.95] (Ions score 20) 30 - 41 728.3412 1454.6679 1454.6244 0.0436 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 28) 30 - 41 728.3452 1454.6759 1454.6244 0.0515 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 44) 30 - 41 728.3458 1454.6771 1454.6244 0.0527 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 59) 30 - 41 728.3463 1454.6781 1454.6244 0.0537 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 64) 30 - 41 728.3469 1454.6793 1454.6244 0.0549 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 61) 30 - 41 728.3476 1454.6806 1454.6244 0.0563 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 36) 30 - 41 728.3478 1454.6810 1454.6244 0.0566 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 56) 30 - 41 728.3491 1454.6836 1454.6244 0.0592 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 62) 30 - 41 728.3498 1454.6851 1454.6244 0.0608 0 K.ELLCPVCQEMYK.Q Lys->Gln (K) [-0.04] (Ions score 48) 30 - 41 728.3457 1454.6768 1454.6971 -0.0203 0 K.ELLCPVCQEMYK.Q Gln->Lys (Q) [+0.04] (Ions score 64) 30 - 41 728.8474 1455.6801 1455.6448 0.0354 0 K.ELLCPVCQEMYK.Q Deamidated (NQ) [+0.98] (Ions score 33) 30 - 41 728.8476 1455.6806 1455.6448 0.0359 0 K.ELLCPVCQEMYK.Q Deamidated (NQ) [+0.98] (Ions score 43) 30 - 41 735.3379 1468.6612 1468.6764 -0.0152 0 K.ELLCPVCQEMYK.Q Methyl (DE) [+14.02] (Ions score 20) 30 - 41 736.3430 1470.6714 1470.6193 0.0521 0 K.ELLCPVCQEMYK.Q Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 40) 30 - 41 736.3433 1470.6721 1470.6193 0.0528 0 K.ELLCPVCQEMYK.Q Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 48) 30 - 41 736.3440 1470.6734 1470.6193 0.0541 0 K.ELLCPVCQEMYK.Q Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 41) 30 - 41 736.3441 1470.6735 1470.6193 0.0543 0 K.ELLCPVCQEMYK.Q Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 44) 30 - 41 736.3449 1470.6753 1470.6193 0.0560 0 K.ELLCPVCQEMYK.Q Oxidation (M); Lys->Gln (K) [-0.04] (Ions score 40) 30 - 41 736.3425 1470.6704 1470.6557 0.0147 0 K.ELLCPVCQEMYK.Q Oxidation (C) [+15.99] (Ions score 31) 30 - 41 736.3434 1470.6722 1470.6557 0.0165 0 K.ELLCPVCQEMYK.Q Oxidation (C) [+15.99] (Ions score 50) 30 - 41 743.3267 1484.6389 1484.6349 0.0040 0 K.ELLCPVCQEMYK.Q Val->Glu (V) [+29.97] (Ions score 31) 30 - 41 744.3413 1486.6681 1486.6328 0.0352 0 K.ELLCPVCQEMYK.Q Sulfide (C) [+31.97] (Ions score 32) 30 - 41 749.8487 1497.6828 1497.6666 0.0163 0 K.ELLCPVCQEMYK.Q Carbamyl (C) [+43.01] (Ions score 52) 30 - 41 749.8491 1497.6836 1497.6666 0.0170 0 K.ELLCPVCQEMYK.Q Carbamyl (C) [+43.01] (Ions score 54) 30 - 41 752.3413 1502.6681 1502.6455 0.0226 0 K.ELLCPVCQEMYK.Q Trioxidation (C) [+47.98] (Ions score 62) 30 - 41 757.8477 1513.6809 1513.6615 0.0194 0 K.ELLCPVCQEMYK.Q Oxidation (M); Carbamyl (C) [+43.01] (Ions score 49) 30 - 41 760.3350 1518.6554 1518.6404 0.0150 0 K.ELLCPVCQEMYK.Q Oxidation (M); Trioxidation (C) [+47.98] (Ions score 49) 30 - 41 760.3359 1518.6573 1518.6404 0.0169 0 K.ELLCPVCQEMYK.Q Oxidation (M); Trioxidation (C) [+47.98] (Ions score 44) 30 - 41 788.3387 1574.6628 1574.6853 -0.0224 0 K.ELLCPVCQEMYK.Q DTT_C (C) [+120.02] (Ions score 21) 30 - 41 788.3391 1574.6635 1574.6853 -0.0217 0 K.ELLCPVCQEMYK.Q DTT_C (C) [+120.02] (Ions score 26) 30 - 41 789.3483 1576.6821 1576.6855 -0.0034 0 K.ELLCPVCQEMYK.Q 2 Carboxymethyl (C); Label:13C(5)15N(1) (V) [+6.01] (Ions score 23) 30 - 41 789.3492 1576.6839 1576.6855 -0.0016 0 K.ELLCPVCQEMYK.Q 2 Carboxymethyl (C); Label:13C(5)15N(1) (V) [+6.01] (Ions score 32) 30 - 41 797.3444 1592.6742 1592.6804 -0.0063 0 K.ELLCPVCQEMYK.Q 2 Carboxymethyl (C); Oxidation (M); Label:13C(5)15N(1) (V) [+6.01] (Ions score 22) 30 - 41 862.3345 1722.6545 1722.8646 -0.2101 0 K.ELLCPVCQEMYK.Q Carboxymethyl (C); Myristoyl (C) [+210.20] (Ions score 25) 108 - 117 357.1857 1068.5354 1068.4989 0.0365 1 K.SGFGTYPGRK.R Lys->Gln (K) [-0.04] (Ions score 24) 108 - 117 535.2751 1068.5356 1068.4989 0.0367 1 K.SGFGTYPGRK.R Lys->Gln (K) [-0.04] (Ions score 39) 108 - 117 357.1859 1068.5358 1068.4989 0.0369 1 K.SGFGTYPGRK.R Lys->Gln (K) [-0.04] (Ions score 18) 108 - 117 535.2752 1068.5359 1068.4989 0.0370 1 K.SGFGTYPGRK.R Lys->Gln (K) [-0.04] (Ions score 29) 108 - 117 575.2581 1148.5017 1148.4652 0.0365 1 K.SGFGTYPGRK.R Phospho (ST); Lys->Gln (K) [-0.04] (Ions score 37) 108 - 117 575.2581 1148.5017 1148.4652 0.0365 1 K.SGFGTYPGRK.R Phospho (ST); Lys->Gln (K) [-0.04] (Ions score 25) 108 - 117 383.8413 1148.5019 1148.4652 0.0367 1 K.SGFGTYPGRK.R Phospho (ST); Lys->Gln (K) [-0.04] (Ions score 26) 108 - 117 409.2196 1224.6371 1224.6503 -0.0132 1 K.SGFGTYPGRK.R HNE (CHK) [+156.12] (Ions score 27) 118 - 142 902.4590 2704.3551 2704.3061 0.0490 1 K.RGALHPQTILFPCPACQGDVELGER.G Carboxymethyl (C); Phe->Ser (F) [-60.04] (Ions score 30) 118 - 142 921.8012 2762.3816 2762.3996 -0.0180 1 K.RGALHPQTILFPCPACQGDVELGER.G 2 Ethyl (E) [+28.03] (Ions score 35) ID TRI46_MOUSE STANDARD; PRT; 759 AA. AC Q7TNM2; Q60717; Q6P1I9; DT 01-FEB-2005 (Rel. 46, Created) DT 01-FEB-2005 (Rel. 46, Last sequence update) DT 10-MAY-2005 (Rel. 47, Last annotation update) DE Tripartite motif protein 46 (Tripartite, fibronectin type-III and C- DE terminal SPRY motif protein). GN Name=Trim46; Synonyms=Trific; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE (ISOFORM 1). RC STRAIN=C57BL/6; RA Short K.M., Cox T.C.; RT "The COS box: a novel coiled-coil associated signature for a new RT subgroup of RBCC proteins."; RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC STRAIN=C57BL/6; TISSUE=Brain; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [3] RP NUCLEOTIDE SEQUENCE OF 630-759 (ISOFORM 1). RC STRAIN=BALB/c; RX MEDLINE=96169575; PubMed=8597643; RA Vos H.L., Mockensturm-Wilson M., Rood P.M., Maas A.M., Duhig T., RA Gendler S.J., Bornstein P.; RT "A tightly organized, conserved gene cluster on mouse chromosome 3 RT (E3-F1)."; RL Mamm. Genome 6:820-822(1995). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q7TNM2-1; Sequence=Displayed; CC Name=2; CC IsoId=Q7TNM2-2; Sequence=VSP_011985, VSP_011986, VSP_011987; CC Note=No experimental confirmation available; CC -!- SIMILARITY: Belongs to the TRIM/RBCC family. CC -!- SIMILARITY: Contains 1 B box-type zinc finger. CC -!- SIMILARITY: Contains 1 B30.2-like domain. CC -!- SIMILARITY: Contains 1 fibronectin type-III domain. CC -!- SIMILARITY: Contains 2 RING-type zinc fingers. CC -------------------------------------------------------------------------- CC This Swiss-Prot entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use as long as its content is in no way modified and this statement is not CC removed. CC -------------------------------------------------------------------------- DR EMBL; AY251388; AAP51208.1; -; mRNA. DR EMBL; BC065049; AAH65049.1; ALT_INIT; mRNA. DR EMBL; U16175; AAA98539.1; -; Genomic_DNA. DR Ensembl; ENSMUSG00000042766; Mus musculus. DR MGI; MGI:2673000; Trim46. DR InterPro; IPR001870; B302. DR InterPro; IPR003961; FN_III. DR InterPro; IPR000315; Znf_Bbox. DR InterPro; IPR001841; Znf_ring. DR Pfam; PF00041; fn3; 1. DR Pfam; PF00643; zf-B_box; 1. DR Pfam; PF00097; zf-C3HC4; 1. DR SMART; SM00336; BBOX; 1. DR SMART; SM00060; FN3; 1. DR SMART; SM00184; RING; 1. DR PROSITE; PS50853; FN3; 1. DR PROSITE; PS50119; ZF_BBOX; 1. DR PROSITE; PS00518; ZF_RING_1; 1. DR PROSITE; PS50089; ZF_RING_2; FALSE_NEG. KW Alternative splicing; Coiled coil; Metal-binding; Zinc; Zinc-finger. FT DOMAIN 430 525 Fibronectin type-III. FT DOMAIN 539 728 B30.2-like. FT ZN_FING 33 73 RING-type 1 (degenerate). FT ZN_FING 172 231 RING-type 2 (degenerate). FT ZN_FING 222 263 B box-type. FT COILED 322 400 Potential. FT VARSPLIC 1 23 MAEGEDMQTFTSIMDALVRISTS -> MGGALEINAINPEM FT EGWRQTS (in isoform 2). FT /FTId=VSP_011985. FT VARSPLIC 429 447 VPEAPVIDTQRTFAYDQIF -> GCGHRGLCSGAPQCPRRP FT S (in isoform 2). FT /FTId=VSP_011986. FT VARSPLIC 448 759 Missing (in isoform 2). FT /FTId=VSP_011987. FT CONFLICT 743 743 G -> A (in Ref. 3). SQ SEQUENCE 759 AA; 83432 MW; 8126C4C79841A536 CRC64; MAEGEDMQTF TSIMDALVRI STSMKNMEKE LLCPVCQEMY KQPLVLPCTH NVCQACAREV LGQQGYIGHG GDPSSEPTSP ASTPSTRSPR LSRRTLPKPD RLDRLLKSGF GTYPGRKRGA LHPQTILFPC PACQGDVELG ERGLSGLFRN LTLERVVERY RQSVSVGGAI LCQLCKPPPL EATKGCTECR ATFCNECFKL FHPWGTQKAQ HEPTLPTLSF RPKGLMCPDH KEEVTHYCKT CQRLVCQLCR VRRTHSGHKI TPVLSAYQAL KDKLTKSLAY ILGNQDTVQT QICELEETIR HTEVSGQQAK EEVSQLVRGL GAVLEEKRAS LLQAIEECQQ ERLSRLSAQI HEHQSLLDGS GLVGYAQEVL KETDQPCFVQ AAKQLHNRIA RATEALQTFR PAASSSFRHC QLDVGREMKL LTELSFLRVP EAPVIDTQRT FAYDQIFLCW RLPPHSPPAW HYTVEFRRTD VPAQPGPTRW QRREEVRGTS ALLENPDTGS VYVLRVRGCN KAGYGEYSED VHLHTPPAPV LHFFLDGRWG ASRERLAISK DQRAVRSIPG LPLLLAAERL LTGCHLSVDV VLGDVAVTQG RSYWACAVDP ASYLVKVGVG LESKLQESFQ GAPDVISPRY DPDSGHDSGA EDAAVEALPP FAFLTIGMGK ILLGSGASSN AGLTGRDGPT ASCTVPLPPR LGICLDYERG RVSFLDAVSF RGLLECPLDC SGPVCPAFCF IGGGAVQLQE PVGTKPERKV TIGGFAKLD
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