Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param Tak1_Blue_00min_1 Tak1_Blue 0 min 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 3682 2771 0 2803 2739 0 64 1084 1077 0 7 38.67 39.32 NaN 10.94 573 141153 1999 1.42 534 26.71 12653 1772 1770 14 18.39 646 36.46 + 0.17453 0.24953 0.12587 0.17835 Tak1_Blue_00min_2 Tak1_Blue 0 min 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4267 3263 0 3375 3151 0 224 1593 1563 0 30 47.2 49.6 NaN 13.39 820 159699 2432 1.52 599 24.63 13557 2165 2157 15.97 21.69 695 32.1 + 0.21389 0.2794 0.15184 0.20384 Tak1_Blue_00min_3 Tak1_Blue 0 min 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4263 3331 0 3439 3223 0 216 1544 1525 0 19 44.9 47.32 NaN 8.8 801 164955 2517 1.53 633 25.15 14406 2252 2247 15.63 20.76 726 32.24 + 0.2759 0.35007 0.19563 0.24988 Tak1_Blue_10min_1 Tak1_Blue 10 min 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4256 3419 0 3533 3305 0 228 1804 1784 0 20 51.06 53.98 NaN 8.77 900 164440 2521 1.53 628 24.91 13985 2221 2213 15.88 22.19 747 33.63 + 0.20507 0.28373 0.143 0.19663 Tak1_Blue_10min_2 Tak1_Blue 10 min 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4191 3731 0 3851 3611 0 240 1805 1777 0 28 46.87 49.21 NaN 11.67 960 161548 2834 1.75 666 23.5 14517 2531 2524 17.43 23.39 785 31.02 + 0.22424 0.32168 0.15495 0.23531 Tak1_Blue_10min_3 Tak1_Blue 10 min 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4215 3653 0 3763 3543 0 220 1777 1753 0 24 47.22 49.48 NaN 10.91 922 165379 2808 1.7 651 23.18 14706 2498 2491 16.99 22.57 790 31.63 + 0.18581 0.2466 0.12985 0.17697 Tak1_Blue_30min_1 Tak1_Blue 30 min 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4253 3397 0 3515 3279 0 236 1711 1686 0 25 48.68 51.42 NaN 10.59 883 164839 2543 1.54 623 24.5 14198 2265 2260 15.95 21.05 720 31.79 + 0.21006 0.27318 0.14952 0.19785 Tak1_Blue_30min_2 Tak1_Blue 30 min 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4214 3417 0 3528 3306 0 222 1698 1673 0 25 48.13 50.6 NaN 11.26 884 161162 2605 1.62 621 23.84 14159 2340 2332 16.53 21.56 725 30.98 + 0.20457 0.2703 0.14504 0.19687 Tak1_Blue_30min_3 Tak1_Blue 30 min 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4302 3063 0 3151 2975 0 176 1426 1409 0 17 45.26 47.36 NaN 9.66 755 165044 2330 1.41 559 23.99 13452 2084 2078 15.49 20.55 655 31.43 + 0.23303 0.2973 0.16862 0.21778 Tak1_DCMU_Blue_00min_1 Tak1_DCMU 0 min 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4307 3079 0 3196 2962 0 234 1441 1421 0 20 45.09 47.97 NaN 8.55 746 164764 2225 1.35 604 27.15 13290 1959 1952 14.74 20.77 686 35.02 + 0.18029 0.23724 0.13094 0.18022 Tak1_DCMU_Blue_00min_2 Tak1_DCMU 0 min 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4305 2731 0 2793 2669 0 124 1370 1360 0 10 49.05 50.96 NaN 8.06 698 157580 2067 1.31 506 24.48 12780 1844 1839 14.43 19.6 604 32.75 + 0.20092 0.2726 0.147 0.20639 Tak1_DCMU_Blue_00min_3 Tak1_DCMU 0 min 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4338 2582 0 2657 2507 0 150 1202 1191 0 11 45.24 47.51 NaN 7.33 592 162608 1977 1.22 450 22.76 13037 1752 1750 13.44 17.88 542 30.94 + 0.18238 0.24451 0.13392 0.18064 Tak1_DCMU_Blue_10min_1 Tak1_DCMU 10 min 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4343 2821 0 2915 2727 0 188 1360 1348 0 12 46.66 49.43 NaN 6.38 755 165525 2163 1.31 490 22.65 13621 1925 1916 14.13 19.1 582 30.23 + 0.20032 0.26171 0.14795 0.19483 Tak1_DCMU_Blue_10min_2 Tak1_DCMU 10 min 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4217 4648 0 5014 4282 0 732 1248 1234 0 14 24.89 28.82 NaN 1.91 578 169480 2580 1.52 878 34.03 13770 2155 2129 15.65 19.98 920 42.69 + 0.22255 0.29139 0.16783 0.2227 Tak1_DCMU_Blue_10min_3 Tak1_DCMU 10 min 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4295 2763 0 2842 2684 0 158 1321 1308 0 13 46.48 48.73 NaN 8.23 681 167720 2137 1.27 477 22.32 13825 1914 1909 13.84 17.95 565 29.52 + 0.2047 0.26704 0.14829 0.1981 Tak1_DCMU_Blue_30min_1 Tak1_DCMU 30 min 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4298 3746 0 4036 3456 0 580 1159 1147 0 12 28.72 33.19 NaN 2.07 599 164381 2364 1.44 692 29.27 12929 1994 1970 15.42 19.62 747 37.46 + 0.19066 0.24136 0.14274 0.18624 Tak1_DCMU_Blue_30min_2 Tak1_DCMU 30 min 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4305 2893 0 2984 2802 0 182 1394 1382 0 12 46.72 49.32 NaN 6.59 723 161229 2216 1.37 506 22.83 13178 1983 1978 15.05 19.55 599 30.21 + 0.18632 0.242 0.1369 0.18224 Tak1_DCMU_Blue_30min_3 Tak1_DCMU 30 min 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 4330 2536 0 2589 2483 0 106 1187 1176 0 11 45.85 47.36 NaN 10.38 624 162219 1964 1.21 446 22.71 12532 1782 1780 14.22 18.26 521 29.24 + 0.19443 0.26075 0.14789 0.20883 Total 76381 57844 0 59984 55704 0 4280 26124 25814 0 310 43.55 46.34 NaN 7.24 1773 2923725 244595 37436 37295 15.31 20.31 12255 32.74 0.20628 0.27509 0.14861 0.20247