Parameter Value Version 1.4.1.2 Fixed modifications Carbamidomethyl (C) Decoy mode revert Special AAs KR Include contaminants True MS/MS tol. (FTMS) 20 ppm Top MS/MS peaks per 100 Da. (FTMS) 12 MS/MS deisotoping (FTMS) True MS/MS tol. (ITMS) 0.5 Da Top MS/MS peaks per 100 Da. (ITMS) 8 MS/MS deisotoping (ITMS) False MS/MS tol. (TOF) 0.1 Da Top MS/MS peaks per 100 Da. (TOF) 10 MS/MS deisotoping (TOF) False MS/MS tol. (Unknown) 0.5 Da Top MS/MS peaks per 100 Da. (Unknown) 10 MS/MS deisotoping (Unknown) False PSM FDR 0.01 Protein FDR 0.01 Site FDR 0.01 Use Normalized Ratios For Occupancy True Min. peptide Length 7 Min. score for unmodified peptides 0 Min. score for modified peptides 40 Min. delta score for unmodified peptides 0 Min. delta score for modified peptides 17 Min. unique peptides 0 Min. razor peptides 1 Min. peptides 1 Use only unmodified peptides and True Modifications included in protein quantification Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) Peptides used for protein quantification Razor Discard unmodified counterpart peptides True Min. ratio count 2 Site quantification Use least modified peptide Re-quantify True Use delta score False iBAQ False iBAQ log fit False MS/MS recalibration False Match between runs True Matching time window [min] 2 Alignment time window [min] 20 Find dependent peptides False Fasta file E:\Sequence\uniprot-Human-Reviewed-20131021-2013_10.fasta Labeled amino acid filtering True Site tables Deamidation (NQ)Sites.txt;Oxidation (M)Sites.txt Cut peaks True Decoy mode revert Special AAs KR Include contaminants True RT shift False Advanced ratios False AIF correlation 0.47 First pass AIF correlation 0.8 AIF topx 20 AIF min mass 0 AIF SIL weight 4 AIF ISO weight 2 AIF iterative True AIF threshold FDR 0.01