Raw file Instrument Tune file Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Variable modifications first search Use variable modifications first search Multiplicity Max. missed cleavages Max. labeled AAs Labels0 Labels1 LC-MS run type Time-dependent recalibration MS MS/MS MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS on Polymers MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Multiplets Multiplets z=1 Multiplets z=2 Multiplets z=3 Multiplets z=4 Multiplets z=5 Multiplets z=6 Multiplets z=7 Multiplets Sequenced Multiplets Sequenced [%] Multiplets Repeatedly Sequenced Multiplets Repeatedly Sequenced [%] Multiplets Identified Multiplets Identified [%] Recalibrated Av. Absolute Mass Deviation Mass Standard Deviation Label free norm param 131025-12hr_IBMX_30mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3700 21522 22373 1430 19187 1756 0 1545 130 1400 15 6.91 9.09 7.3 0.85 1155 833972 20276 2.43 1041 5.13 137026 18894 18784 13.79 15.5 1490 7.89 4705 73 2945 1474 182 21 7 3 1100 23.38 269 24.45 95 8.64 + 1.1506 1.3662 131025-12hr_IBMX_30mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3722 21366 22139 1453 19084 1602 0 1523 136 1367 20 6.88 9.36 7.16 1.25 1145 835537 20152 2.41 1003 4.98 136001 18871 18764 13.88 15.57 1432 7.59 4649 57 2920 1432 205 25 8 2 1135 24.41 274 24.14 104 9.16 + 1.1959 1.3265 131025-12hr_IBMX_30mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3741 21301 22158 1413 18981 1764 0 1555 130 1413 12 7.02 9.2 7.44 0.68 1171 813959 20010 2.46 1085 5.42 132831 18617 18497 14.02 15.73 1541 8.28 4603 83 2880 1445 166 19 7 3 1087 23.62 268 24.66 97 8.92 + 1.2479 1.4217 131025-12hr_IBMX_60mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3673 21273 22204 1265 19055 1884 0 2148 180 1941 27 9.67 14.23 10.19 1.43 1494 852378 19915 2.34 994 4.99 132380 18423 18316 13.92 15.36 1431 7.77 4316 50 1959 1913 333 46 10 5 991 22.96 228 23.01 131 13.22 + 0.93571 1.1472 131025-12hr_IBMX_60mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3676 21225 22126 1305 18993 1828 0 2136 185 1929 22 9.65 14.18 10.16 1.2 1507 853446 19827 2.32 1049 5.29 132747 18398 18303 13.86 15.28 1448 7.87 4359 44 1948 1992 314 48 10 3 997 22.87 248 24.87 123 12.34 + 0.92281 1.0758 131025-12hr_IBMX_60mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3747 21123 21967 1243 19010 1714 0 1934 179 1737 18 8.8 14.4 9.14 1.05 1384 846552 19849 2.34 979 4.93 133012 18485 18410 13.9 15.35 1392 7.53 4339 79 1985 1898 319 48 7 3 936 21.57 223 23.82 123 13.14 + 0.89017 1.0782 131025-12hr_IBMX_90mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3763 20418 21328 1102 18402 1824 0 2286 173 2097 16 10.72 15.7 11.4 0.88 1652 756984 19460 2.57 826 4.24 120693 18039 17943 14.95 16.46 1269 7.03 3621 50 1578 1676 276 32 7 2 854 23.58 202 23.65 133 15.57 + 1.1983 1.3471 131025-12hr_IBMX_90mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3745 20433 21281 1048 18525 1708 0 2303 175 2111 17 10.82 16.7 11.4 1 1671 760891 19484 2.56 810 4.16 120990 18127 18016 14.98 16.47 1247 6.88 3649 51 1595 1668 285 40 8 2 806 22.09 196 24.32 128 15.88 + 1.1157 1.3177 131025-12hr_IBMX_90mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3987 19927 21003 924 17913 2166 0 1880 122 1742 16 8.95 13.2 9.72 0.74 1431 727158 18929 2.6 823 4.35 113593 17287 17190 15.22 16.84 1334 7.72 3172 51 1566 1319 204 28 4 0 732 23.08 157 21.45 90 12.3 + 1.1744 1.3873 131025-12hr_IBMX_200mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3752 20248 21157 1040 18293 1824 0 2319 152 2145 22 10.96 14.62 11.73 1.21 1631 745475 19245 2.58 853 4.43 114741 17741 17651 15.46 17.01 1346 7.59 3360 46 1228 1622 368 71 20 5 779 23.18 196 25.16 108 13.86 + 1.1288 1.2962 131025-12hr_IBMX_200mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3762 20361 21368 1066 18284 2018 0 2254 209 2020 25 10.55 19.61 11.05 1.24 1604 747940 19284 2.58 889 4.61 114386 17692 17576 15.47 16.99 1369 7.74 3315 59 1226 1566 375 71 13 5 791 23.86 204 25.79 157 19.85 + 1.1174 1.3071 131025-12hr_IBMX_200mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4035 19571 20674 819 17639 2216 0 1994 100 1877 17 9.64 12.21 10.64 0.77 1472 733583 18567 2.53 858 4.62 107889 16828 16700 15.6 17.33 1367 8.12 2771 59 1134 1263 258 43 12 2 637 22.99 134 21.04 79 12.4 + 1.1639 1.3393 131025-12hr_IBMX_500mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3798 20300 21421 1017 18158 2246 0 2446 174 2251 21 11.42 17.11 12.4 0.93 1688 745978 19265 2.58 887 4.6 110377 17465 17366 15.82 17.5 1459 8.35 3130 50 1067 1430 460 98 21 4 731 23.35 201 27.5 122 16.69 + 0.99569 1.1611 131025-12hr_IBMX_500mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3830 20216 21262 1027 18131 2104 0 2304 171 2107 26 10.84 16.65 11.62 1.24 1631 750078 19198 2.56 872 4.54 110311 17446 17346 15.82 17.48 1453 8.33 3053 52 1022 1451 422 82 21 3 707 23.16 226 31.97 119 16.83 + 1.0303 1.18 131025-12hr_IBMX_500mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4136 19403 20713 819 17270 2624 0 2137 119 1991 27 10.32 14.53 11.53 1.03 1527 753100 18406 2.44 834 4.53 104738 16500 16386 15.75 17.56 1361 8.25 2621 46 973 1187 322 76 16 1 609 23.24 156 25.62 86 14.12 + 1.1171 1.3141 131025-12hr_IBMX_FT-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4340 18872 20313 958 16423 2932 0 519 5 508 6 2.56 0.52 3.09 0.2 283 483099 16981 3.52 1673 9.85 65941 13912 13358 21.1 35.23 2949 21.2 1251 256 791 195 9 0 0 0 517 41.33 255 49.32 4 0.77 + 1.0717 1.1654 131025-12hr_IBMX_FT-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4330 18978 20383 968 16547 2868 0 459 12 444 3 2.25 1.24 2.68 0.1 243 479574 16964 3.54 1769 10.43 65787 13907 13329 21.14 35.21 3035 21.82 1258 255 801 188 14 0 0 0 492 39.11 262 53.25 7 1.42 + 0.74057 1.0741 131025-12hr_IBMX_FT-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4954 17310 18951 732 14885 3334 0 380 13 361 6 2.01 1.78 2.43 0.18 213 489476 15435 3.15 1610 10.43 59783 12246 11710 20.48 36.65 2769 22.61 934 229 582 118 5 0 0 0 397 42.51 194 48.87 8 2.02 + 0.89 0.94829 131025-24hr_IBMX_30mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3842 20876 21657 1437 18656 1564 0 1659 167 1478 14 7.66 11.62 7.92 0.9 1241 808925 19664 2.43 1064 5.41 129124 18336 18245 14.2 16.07 1565 8.54 4308 80 2824 1219 156 17 8 4 1099 25.51 274 24.93 123 11.19 + 0.98515 1.1671 131025-24hr_IBMX_30mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3816 20894 21699 1491 18596 1612 0 1687 159 1504 24 7.77 10.66 8.09 1.49 1223 809372 19640 2.43 1102 5.61 129546 18270 18173 14.1 15.91 1587 8.69 4391 66 2886 1262 148 21 6 2 1123 25.58 291 25.91 116 10.33 + 1.0201 1.1895 131025-24hr_IBMX_30mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3836 20946 21726 1396 18766 1564 0 1538 124 1405 9 7.08 8.88 7.49 0.58 1173 799097 19676 2.46 1118 5.68 128532 18316 18202 14.25 16.12 1602 8.75 4182 72 2800 1141 142 16 7 4 1078 25.78 254 23.56 90 8.35 + 0.98075 1.1368 131025-24hr_IBMX_60mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3762 21158 22105 1258 18935 1912 0 2050 211 1823 16 9.27 16.77 9.63 0.84 1462 845551 19760 2.34 1087 5.5 130633 18257 18128 13.98 15.52 1528 8.37 4085 55 1939 1766 281 38 5 1 957 23.43 216 22.57 142 14.84 + 0.98203 1.1537 131025-24hr_IBMX_60mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3767 21226 22155 1305 18950 1900 0 1972 193 1760 19 8.9 14.79 9.29 1 1436 853883 19887 2.33 1040 5.23 131247 18337 18202 13.97 15.55 1511 8.24 4172 48 1987 1807 290 31 8 1 987 23.66 241 24.42 126 12.77 + 1.0034 1.1618 131025-24hr_IBMX_60mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3767 21126 21999 1277 18928 1794 0 2162 168 1979 15 9.83 13.16 10.46 0.84 1581 828085 19823 2.39 1084 5.47 128698 18341 18236 14.25 15.78 1568 8.55 3980 64 1925 1727 222 30 10 2 962 24.17 232 24.12 119 12.37 + 0.95329 1.1497 131025-24hr_IBMX_90mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3845 20069 21132 997 18001 2134 0 2038 131 1880 27 9.64 13.14 10.44 1.27 1499 726489 18927 2.61 983 5.19 110712 17206 17097 15.54 17.24 1530 8.89 2989 45 1494 1256 170 17 6 1 766 25.63 171 22.32 99 12.92 + 1.192 1.3568 131025-24hr_IBMX_90mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3869 20082 21087 994 18071 2022 0 2088 128 1946 14 9.9 12.88 10.77 0.69 1548 725006 18943 2.61 976 5.15 110777 17226 17119 15.55 17.21 1548 8.99 3068 49 1491 1312 186 20 8 2 762 24.84 170 22.31 92 12.07 + 1.0582 1.2133 131025-24hr_IBMX_90mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4156 19104 20214 821 17167 2226 0 1765 96 1655 14 8.73 11.69 9.64 0.63 1354 698236 18001 2.58 916 5.09 103331 16310 16186 15.78 17.56 1426 8.74 2542 49 1361 994 125 10 2 1 646 25.41 135 20.9 68 10.53 + 1.202 1.3731 131025-24hr_IBMX_200mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3836 20111 21030 976 18208 1846 0 2175 141 2007 27 10.34 14.45 11.02 1.46 1605 722141 18983 2.63 978 5.15 109343 17313 17190 15.83 17.47 1584 9.15 2953 62 1203 1377 262 39 9 1 727 24.62 185 25.45 110 15.13 + 1.1087 1.2469 131025-24hr_IBMX_200mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3822 20045 20970 952 18168 1850 0 2200 147 2030 23 10.49 15.44 11.17 1.24 1591 717846 18877 2.63 1021 5.41 107677 17228 17119 16 17.64 1598 9.28 2844 45 1078 1374 290 42 12 3 712 25.04 182 25.56 107 15.03 + 0.90857 1.1109 131025-24hr_IBMX_200mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4129 19180 20266 790 17294 2182 0 1855 116 1713 26 9.15 14.68 9.91 1.19 1422 695784 18088 2.6 922 5.1 99934 16348 16235 16.36 18.17 1455 8.9 2416 54 1039 1101 190 29 3 0 618 25.58 128 20.71 93 15.05 + 1.1969 1.3897 131025-24hr_IBMX_500mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4021 19841 20981 989 17712 2280 0 2357 222 2116 19 11.23 22.45 11.95 0.83 1557 761462 18574 2.44 1098 5.91 101006 16580 16436 16.41 18.7 1743 10.51 2622 76 888 1224 342 76 14 2 679 25.9 219 32.25 140 20.62 + 0.97024 1.1551 131025-24hr_IBMX_500mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4014 19800 20952 961 17681 2310 0 2344 181 2144 19 11.19 18.83 12.13 0.82 1587 763567 18557 2.43 1104 5.95 100697 16553 16407 16.44 18.7 1720 10.39 2503 75 855 1157 331 72 11 2 665 26.57 212 31.88 122 18.35 + 1.0182 1.1895 131025-24hr_IBMX_500mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4284 19052 20263 817 17024 2422 0 2265 117 2115 33 11.18 14.32 12.42 1.36 1562 794893 17838 2.24 1062 5.95 97144 15758 15630 16.22 18.78 1700 10.79 2209 88 766 1009 284 51 11 0 572 25.89 159 27.8 80 13.99 + 1.2056 1.4026 131025-24hr_IBMX_FT-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4202 19125 20572 1067 16567 2938 0 493 9 481 3 2.4 0.84 2.9 0.1 263 493709 17041 3.45 1786 10.48 67062 14177 13660 21.14 34.26 2855 20.14 1302 241 843 206 10 1 1 0 575 44.16 286 49.74 8 1.39 + 1.1405 1.3426 131025-24hr_IBMX_FT-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4152 19447 20961 1115 16762 3084 0 519 11 502 6 2.48 0.99 2.99 0.19 274 501159 17334 3.46 1773 10.23 69329 14455 13851 20.85 33.82 2853 19.74 1373 280 883 198 11 1 0 0 601 43.77 305 50.75 8 1.33 + 0.77625 0.89179 131025-24hr_IBMX_FT-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 5530 12810 14922 455 10083 4384 0 164 2 157 5 1.1 0.44 1.56 0.11 95 442079 11069 2.5 1305 11.79 32205 7110 6837 22.08 40.41 2721 38.27 361 118 175 65 3 0 0 0 174 48.2 121 69.54 2 1.15 + 0.56002 0.6642 131025-48hr_IBMX_30mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3866 20968 21745 1343 18838 1564 0 1628 146 1471 11 7.49 10.87 7.81 0.7 1208 820549 19709 2.4 1104 5.6 130926 18358 18250 14.02 15.81 1642 8.94 4310 85 2635 1376 183 20 10 1 1026 23.81 258 25.15 105 10.23 + 1.157 1.3489 131025-48hr_IBMX_30mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3841 20991 21732 1436 18792 1504 0 1629 142 1456 31 7.5 9.89 7.75 2.06 1200 815481 19724 2.42 1101 5.58 130760 18454 18353 14.11 15.88 1587 8.6 4338 72 2668 1397 168 20 12 1 1091 25.15 261 23.92 99 9.07 + 0.89864 1.1089 131025-48hr_IBMX_30mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3806 21149 22011 1450 18799 1762 0 1588 130 1438 20 7.21 8.97 7.65 1.14 1174 814682 19821 2.43 1119 5.65 130580 18433 18327 14.12 16.04 1586 8.6 4359 84 2772 1301 167 22 7 6 1108 25.42 282 25.45 92 8.3 + 1.1588 1.3788 131025-48hr_IBMX_60mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3825 20767 21510 1251 18771 1488 0 1998 201 1785 12 9.29 16.07 9.51 0.81 1409 827085 19494 2.36 1046 5.37 129917 18212 18124 14.02 15.43 1490 8.18 4074 57 1875 1820 272 43 5 2 943 23.15 223 23.65 133 14.1 + 1.1082 1.253 131025-48hr_IBMX_60mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3798 20847 21647 1237 18808 1602 0 2029 186 1823 20 9.37 15.04 9.69 1.25 1443 829796 19564 2.36 1053 5.38 129757 18192 18115 14.02 15.44 1523 8.37 4097 53 1932 1761 311 37 3 0 928 22.65 237 25.54 131 14.12 + 0.98075 1.1849 131025-48hr_IBMX_60mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3799 20884 21658 1276 18832 1550 0 1936 183 1730 23 8.94 14.34 9.19 1.48 1350 833380 19648 2.36 1001 5.09 129940 18246 18156 14.04 15.49 1514 8.3 4093 52 1871 1813 303 47 6 1 954 23.31 257 26.94 123 12.89 + 0.97703 1.2001 131025-48hr_IBMX_90mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3910 19897 20870 964 17942 1964 0 1924 131 1778 15 9.22 13.59 9.91 0.76 1441 717760 18732 2.61 979 5.23 109121 17104 17003 15.67 17.34 1543 9.02 2949 45 1438 1240 196 25 4 1 734 24.89 169 23.02 95 12.94 + 1.32 1.5013 131025-48hr_IBMX_90mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4008 19809 20824 949 17837 2038 0 1975 116 1841 18 9.48 12.22 10.32 0.88 1483 711624 18700 2.63 940 5.03 109574 17040 16939 15.55 17.18 1512 8.87 2968 41 1484 1236 178 24 4 1 720 24.26 179 24.86 85 11.81 + 1.1306 1.2737 131025-48hr_IBMX_90mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4022 19822 20779 917 17942 1920 0 1992 114 1853 25 9.59 12.43 10.33 1.3 1462 719655 18740 2.6 905 4.83 110896 17191 17079 15.5 17.14 1453 8.45 2878 44 1476 1171 161 21 3 2 703 24.43 160 22.76 87 12.38 + 1.076 1.288 131025-48hr_IBMX_200mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3979 19846 20898 821 17965 2112 0 2099 147 1939 13 10.04 17.9 10.79 0.62 1494 739277 18709 2.53 980 5.24 107269 16956 16827 15.81 17.51 1567 9.24 2654 51 1135 1178 253 28 8 1 609 22.95 153 25.12 98 16.09 + 1.0847 1.2355 131025-48hr_IBMX_200mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3973 19940 20997 895 17980 2122 0 1979 145 1813 21 9.43 16.2 10.08 0.99 1454 746969 18824 2.52 959 5.09 107687 17067 16953 15.85 17.58 1538 9.01 2686 50 1068 1248 266 41 12 1 665 24.76 172 25.86 102 15.34 + 1.1054 1.2965 131025-48hr_IBMX_200mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3981 19851 20936 859 17891 2186 0 1882 124 1732 26 8.99 14.44 9.68 1.19 1395 752954 18712 2.49 999 5.34 108095 16995 16876 15.72 17.5 1513 8.9 2630 55 1109 1194 232 30 9 1 659 25.06 158 23.98 98 14.87 + 1.1501 1.3553 131025-48hr_IBMX_500mM-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4065 19517 20751 856 17387 2508 0 2033 140 1858 35 9.8 16.36 10.69 1.4 1430 743923 18332 2.46 1013 5.53 101352 16416 16301 16.2 18.1 1585 9.66 2408 46 847 1129 313 56 16 1 646 26.83 159 24.61 102 15.79 + 1.0664 1.2344 131025-48hr_IBMX_500mM-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4063 19376 20603 834 17277 2492 0 2044 120 1893 31 9.92 14.39 10.96 1.24 1466 722254 18202 2.52 1016 5.58 98513 16239 16131 16.48 18.43 1597 9.83 2278 52 782 1056 322 52 12 2 605 26.56 162 26.78 84 13.88 + 1.1333 1.3022 131025-48hr_IBMX_500mM-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 3985 19811 21170 859 17453 2858 0 1968 123 1819 26 9.3 14.32 10.42 0.91 1414 745398 18526 2.49 1048 5.66 100927 16430 16324 16.28 18.27 1608 9.79 2387 53 810 1123 318 63 17 3 622 26.06 170 27.33 85 13.67 + 1.0614 1.2519 131025-48hr_IBMX_FT-01 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4467 18552 20108 938 16012 3158 0 471 4 456 11 2.34 0.43 2.85 0.35 277 477629 16569 3.47 1722 10.39 61703 13341 12831 21.62 35.87 3032 22.73 1124 220 736 158 10 0 0 0 479 42.62 257 53.65 4 0.84 + 1.0417 1.1443 131025-48hr_IBMX_FT-02 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4490 18486 20081 866 15975 3240 0 479 5 471 3 2.39 0.58 2.95 0.09 273 474166 16540 3.49 1690 10.22 61021 13264 12721 21.74 36.33 3028 22.83 1038 234 645 151 8 0 0 0 466 44.89 224 48.07 3 0.64 + 0.85113 0.98926 131025-48hr_IBMX_FT-03 20131024_tipcolumn_tune-1uscans Trypsin/P Specific FALSE Acetyl (Protein N-term);Oxidation (M);Deamidation (NQ) FALSE 2 2 3 DimethLys0;DimethNter0 DimethLys6;DimethNter6 Standard 4409 18996 20505 1099 16360 3046 0 470 11 456 3 2.29 1 2.79 0.1 264 490211 17090 3.49 1659 9.71 66773 13974 13381 20.93 35.17 2936 21.01 1257 289 769 190 9 0 0 0 584 46.46 296 50.68 6 1.03 + 0.72133 0.91279 IBMX12h 8368 IBMX24h 8268 IBMX48h 7788 Total 215628 1078248 1136337 57577 961210 117550 0 93577 6856 85748 973 8.23 11.91 8.92 0.83 11417 39179257 5825034 906911 897289 15.57 17.87 94040 10.37 163934 4740 80689 64574 11630 1788 420 93 41543 25.34 11451 27.56 4891 11.77 1.0688 1.2525