MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	CRC_phospho_iTRAQ_JPST000204
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20190823\20190823213658333178^10.242.132.48^taba@jp\PeakList.PeakListMergePreMS2_CH_1\CRC_phospho_iTRAQ_04.HCD.mqWzd.txt
MTD	ms_run[2]-location	D:\JobRequest\ResultFiles\20190823\20190823213658333178^10.242.132.48^taba@jp\Psearch.MaxQuantExec1\CRC_phospho_iTRAQ_04.maxqFin.txt
MTD	software[1]	[MS, MS:1001583, MaxQuant, 1.6.2.10]
MTD	software[1]-setting	Taxon=userFasta.sprot_human_mix_20181121
MTD	software[1]-setting	enzymes=Trypsin/P
MTD	software[1]-setting	enzymeMode=0
MTD	software[1]-setting	fixedModifications=Carbamidomethyl (C),iTRAQ4plex (K),iTRAQ4plex (N-term)
MTD	software[1]-setting	variableModifications=iTRAQ4plex (Y),Oxidation (M),Phospho (STY)
MTD	software[1]-setting	maxMissedCleavages=2
MTD	software[1]-setting	lcmsRunType=Standard
MTD	software[1]-setting	firstSearchTol=20
MTD	software[1]-setting	mainSearchTol=4.5
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.sprot_human_mix_20181121
MTD	software[2]-setting	CLE=[RK]|{}
MTD	software[2]-setting	MODS=Carbamidomethyl (C),iTRAQ4plex (K),iTRAQ4plex (N-term)
MTD	software[2]-setting	IT_MODS=Oxidation (M),Phospho (S),Phospho (T),Phospho (Y),iTRAQ4plex (Y)
MTD	software[2]-setting	TOL(-)=20
MTD	software[2]-setting	TOL(+)=20
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=5
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=2
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.sprot_human_mix_20181121
MTD	software[3]-setting	search_enzyme_number=2
MTD	software[3]-setting	FixMod=Carbamidomethyl (C),iTRAQ4plex (K),iTRAQ4plex (N-term)
MTD	software[3]-setting	VarMod=iTRAQ4plex (Y),Oxidation (M),Phospho (S),Phospho (T),Phospho (Y)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=2
MTD	software[3]-setting	peptide_mass_tolerance=20
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=0.02
MTD	software[3]-setting	fragment_bin_offset=0.0
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:4, Carbamidomethyl,]
MTD	fixed_mod[1]-site	C
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:214, iTRAQ4plex,]
MTD	fixed_mod[2]-site	K
MTD	fixed_mod[2]-position	Anywhere
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:214, iTRAQ4plex,]
MTD	fixed_mod[3]-site	N-term
MTD	fixed_mod[3]-position	Any N-term
MTD	variable_mod[1]	[UNIMOD, UNIMOD:214, iTRAQ4plex,]
MTD	variable_mod[1]-site	Y
MTD	variable_mod[1]-position	Anywhere
MTD	variable_mod[2]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[2]-site	M
MTD	variable_mod[2]-position	Anywhere
MTD	variable_mod[3]	[UNIMOD, UNIMOD:21, Phospho,]
MTD	variable_mod[3]-site	S
MTD	variable_mod[3]-position	Anywhere
MTD	variable_mod[4]	[UNIMOD, UNIMOD:21, Phospho,]
MTD	variable_mod[4]-site	T
MTD	variable_mod[4]-position	Anywhere
MTD	variable_mod[5]	[UNIMOD, UNIMOD:21, Phospho,]
MTD	variable_mod[5]-site	Y
MTD	variable_mod[5]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	sp|O15127|SCAM2_HUMAN	Secretory carrier-associated membrane protein 2 OS=Homo sapiens OX=9606 GN=SCAMP2 PE=1 SV=2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ],[MS, MS:1002251, Comet, ],[MS, MS:1001476, X!Tandem, ]	22.0	null	317-UNIMOD:214,319-UNIMOD:21	0.04	22.0	6	1	0
PRT	sp|Q16204|CCDC6_HUMAN	Coiled-coil domain-containing protein 6 OS=Homo sapiens OX=9606 GN=CCDC6 PE=1 SV=2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ],[MS, MS:1001583, MaxQuant, ],[MS, MS:1001476, X!Tandem, ]	20.0	null	235-UNIMOD:214,240-UNIMOD:21,244-UNIMOD:21	0.03	20.0	3	1	0
PRT	sp|Q3KNT7-3|NSN5B_HUMAN	Isoform 3 of Putative NOL1/NOP2/Sun domain family member 5B OS=Homo sapiens OX=9606 GN=NSUN5P1	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19.0	null	144-UNIMOD:214,149-UNIMOD:4,150-UNIMOD:21,153-UNIMOD:4	0.08	19.0	2	1	0
PRT	sp|Q14247-3|SRC8_HUMAN	Isoform 3 of Src substrate cortactin OS=Homo sapiens OX=9606 GN=CTTN	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19.0	null	362-UNIMOD:214,364-UNIMOD:21,368-UNIMOD:21	0.03	19.0	1	1	1
PRT	sp|P04908|H2A1B_HUMAN	Histone H2A type 1-B/E OS=Homo sapiens OX=9606 GN=HIST1H2AB PE=1 SV=2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ]	19.0	null	101-UNIMOD:214,119-UNIMOD:214	0.15	19.0	1	1	1
PRT	sp|Q15149|PLEC_HUMAN	Plectin OS=Homo sapiens OX=9606 GN=PLEC PE=1 SV=3	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ]	16.0	null	4384-UNIMOD:214,4384-UNIMOD:21,4396-UNIMOD:21	0.00	16.0	1	1	1
PRT	sp|Q9Y4U1|MMAC_HUMAN	Methylmalonic aciduria and homocystinuria type C protein OS=Homo sapiens OX=9606 GN=MMACHC PE=1 SV=3	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	15.0	null	274-UNIMOD:214,275-UNIMOD:21	0.04	15.0	2	1	0
PRT	sp|Q9NSK0-5|KLC4_HUMAN	Isoform 5 of Kinesin light chain 4 OS=Homo sapiens OX=9606 GN=KLC4	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	14.0	null	530-UNIMOD:214,534-UNIMOD:21	0.03	14.0	1	1	1
PRT	sp|Q9Y624-2|JAM1_HUMAN	Isoform 2 of Junctional adhesion molecule A OS=Homo sapiens OX=9606 GN=F11R	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	14.0	null	244-UNIMOD:214,246-UNIMOD:21	0.03	14.0	1	1	1
PRT	sp|Q92597-3|NDRG1_HUMAN	Isoform 3 of Protein NDRG1 OS=Homo sapiens OX=9606 GN=NDRG1	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	14.0	null	247-UNIMOD:214,251-UNIMOD:21,255-UNIMOD:21	0.05	14.0	1	1	1
PRT	sp|O60832|DKC1_HUMAN	H/ACA ribonucleoprotein complex subunit DKC1 OS=Homo sapiens OX=9606 GN=DKC1 PE=1 SV=3	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12.0	null	508-UNIMOD:214,513-UNIMOD:21	0.02	12.0	1	1	1
PRT	sp|P0DMV8-2|HS71A_HUMAN	Isoform 2 of Heat shock 70 kDa protein 1A OS=Homo sapiens OX=9606 GN=HSPA1A	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12.0	null	574-UNIMOD:214,576-UNIMOD:21	0.02	12.0	1	1	1
PRT	sp|Q8N3V7-2|SYNPO_HUMAN	Isoform 2 of Synaptopodin OS=Homo sapiens OX=9606 GN=SYNPO	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12.0	null	893-UNIMOD:214,894-UNIMOD:21,901-UNIMOD:4	0.01	12.0	1	1	1
PRT	sp|P16403|H12_HUMAN	Histone H1.2 OS=Homo sapiens OX=9606 GN=HIST1H1C PE=1 SV=2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12.0	null	160-UNIMOD:214,168-UNIMOD:214	0.05	12.0	1	1	1
PRT	sp|Q99959-2|PKP2_HUMAN	Isoform 1 of Plakophilin-2 OS=Homo sapiens OX=9606 GN=PKP2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12.0	null	148-UNIMOD:214,151-UNIMOD:21,154-UNIMOD:21	0.01	12.0	1	1	1
PRT	sp|P10412|H14_HUMAN	Histone H1.4 OS=Homo sapiens OX=9606 GN=HIST1H1E PE=1 SV=2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001476, X!Tandem, ]	12.0	null	2-UNIMOD:1,2-UNIMOD:21,17-UNIMOD:214	0.08	12.0	1	1	1
PRT	sp|P68871|HBB_HUMAN	Hemoglobin subunit beta OS=Homo sapiens OX=9606 GN=HBB PE=1 SV=2	null	null	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001476, X!Tandem, ]	12.0	null	2-UNIMOD:214,9-UNIMOD:214	0.06	12.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	AASSAAQGAFQGN	1	sp|O15127|SCAM2_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	22	1-UNIMOD:214,3-UNIMOD:21	ms_run[2]:scan=4823	38.655	2	1402.6	1402.6000	R	-	317	330
PSM	LDQPVSAPPSPR	2	sp|Q16204|CCDC6_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:214,6-UNIMOD:21,10-UNIMOD:21	ms_run[1]:scan=5867	46.782968333333336	3	1566.696331	1566.696625	K	D	235	247
PSM	AAAGACTPPCT	3	sp|Q3KNT7-3|NSN5B_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19	1-UNIMOD:214,6-UNIMOD:4,7-UNIMOD:21,10-UNIMOD:4	ms_run[2]:scan=2672	21.855	2	1299.5111	1299.5111	R	-	144	155
PSM	AASSAAQGAFQGN	4	sp|O15127|SCAM2_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19	1-UNIMOD:214,3-UNIMOD:21	ms_run[2]:scan=4693	37.637	2	1402.6	1402.6000	R	-	317	330
PSM	AASSAAQGAFQGN	5	sp|O15127|SCAM2_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19	1-UNIMOD:214,3-UNIMOD:21	ms_run[2]:scan=4964	39.744	2	1402.6	1402.6000	R	-	317	330
PSM	LDQPVSAPPSPR	6	sp|Q16204|CCDC6_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19	1-UNIMOD:214,6-UNIMOD:21,10-UNIMOD:21	ms_run[2]:scan=5921	47.212	2	1566.6966	1566.6966	K	D	235	247
PSM	TQTPPVSPAPQPTEER	7	sp|Q14247-3|SRC8_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	19	1-UNIMOD:214,3-UNIMOD:21,7-UNIMOD:21	ms_run[2]:scan=4221	33.958	3	2037.8932	2037.8932	K	L	362	378
PSM	VTIAQGGVLPNIQAVLLPK	8	sp|P04908|H2A1B_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:214,19-UNIMOD:214	ms_run[1]:scan=18384	152.15397166666665	3	2219.368565	2218.365660	R	K	101	120
PSM	AASSAAQGAFQGN	9	sp|O15127|SCAM2_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:214,3-UNIMOD:21	ms_run[1]:scan=5295	42.32796833333334	2	1403.582473	1402.600009	R	-	317	330
PSM	AASSAAQGAFQGN	10	sp|O15127|SCAM2_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	18	1-UNIMOD:214,3-UNIMOD:21	ms_run[2]:scan=4322	34.759	2	1402.6	1402.6000	R	-	317	330
PSM	SSSVGSSSSYPISPAVSR	11	sp|Q15149|PLEC_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:214,1-UNIMOD:21,13-UNIMOD:21	ms_run[1]:scan=7121	56.6883	3	2057.883348	2057.882982	R	T	4384	4402
PSM	AASSAAQGAFQGN	12	sp|O15127|SCAM2_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001476, X!Tandem, ]	16	1-UNIMOD:214,3-UNIMOD:21	ms_run[1]:scan=5424	43.352138333333336	2	1403.5832	1402.5992	R	-	317	330
PSM	VSPPASPGP	13	sp|Q9Y4U1|MMAC_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	15	1-UNIMOD:214,2-UNIMOD:21	ms_run[2]:scan=5431	43.407	2	1031.4811	1031.4811	R	-	274	283
PSM	GLSASTMDLSSSS	14	sp|Q9NSK0-5|KLC4_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	14	1-UNIMOD:214,5-UNIMOD:21	ms_run[2]:scan=9580	76.355	2	1465.6129	1465.6129	R	-	530	543
PSM	QTSSFLV	15	sp|Q9Y624-2|JAM1_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	14	1-UNIMOD:214,3-UNIMOD:21	ms_run[2]:scan=9738	77.646	2	1004.4702	1004.4702	K	-	244	251
PSM	TASGSSVTSLDGTR	16	sp|Q92597-3|NDRG1_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	14	1-UNIMOD:214,5-UNIMOD:21,9-UNIMOD:21	ms_run[2]:scan=4463	35.856	2	1641.677	1641.6770	R	S	247	261
PSM	LDQPVSAPPSPR	17	sp|Q16204|CCDC6_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001476, X!Tandem, ]	14	1-UNIMOD:214,6-UNIMOD:21,10-UNIMOD:21	ms_run[1]:scan=6063	48.331968333333336	2	1567.6992	1566.6962	K	D	235	247
PSM	AAAGACTPPCT	18	sp|Q3KNT7-3|NSN5B_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,6-UNIMOD:4,7-UNIMOD:21,10-UNIMOD:4	ms_run[2]:scan=2545	20.848	2	1299.5111	1299.5111	R	-	144	155
PSM	EVELVSE	19	sp|O60832|DKC1_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,6-UNIMOD:21	ms_run[2]:scan=7020	55.901	2	1027.4597	1027.4597	K	-	508	515
PSM	GGSGSGPTIEEVD	20	sp|P0DMV8-2|HS71A_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,3-UNIMOD:21	ms_run[2]:scan=6322	50.352	2	1427.5939	1427.5939	K	-	574	587
PSM	GSLPAEASCTT	21	sp|Q8N3V7-2|SYNPO_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,2-UNIMOD:21,9-UNIMOD:4	ms_run[2]:scan=4627	37.126	2	1316.5441	1316.5441	R	-	893	904
PSM	KPAAATVTK	22	sp|P16403|H12_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,9-UNIMOD:214	ms_run[2]:scan=2264	18.593	3	1317.8346	1317.8346	K	K	160	169
PSM	LEISPDSSPER	23	sp|Q99959-2|PKP2_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,4-UNIMOD:21,7-UNIMOD:21	ms_run[2]:scan=6820	54.298	2	1532.6283	1532.6283	R	A	148	159
PSM	VSPPASPGP	24	sp|Q9Y4U1|MMAC_HUMAN	0	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001583, MaxQuant, ]	12	1-UNIMOD:214,2-UNIMOD:21	ms_run[2]:scan=5304	42.399	2	1031.4811	1031.4811	R	-	274	283
PSM	SETAPAAPAAPAPAEK	25	sp|P10412|H14_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001476, X!Tandem, ]	12	1-UNIMOD:1,1-UNIMOD:21,16-UNIMOD:214	ms_run[1]:scan=5360	42.8418	3	1887.9223	1887.9218	M	T	2	18
PSM	VHLTPEEK	26	sp|P68871|HBB_HUMAN	1	userFasta.sprot_human_mix_20181121	userFasta.sprot_human_mix_20181121	[MS, MS:1001476, X!Tandem, ]	12	1-UNIMOD:214,8-UNIMOD:214	ms_run[1]:scan=4332	34.83280166666667	3	1239.7063	1239.7061	M	S	2	10