Sequence Modifications Mass Mass Fractional Part Protein Groups Proteins Unique (Groups) Unique (Proteins) GFP-Chromophore Pyl->PocK Pyl->TCOK Missed cleavages Identification type 2 Identification type 3 Experiment 2 Experiment 3 Retention time Calibrated retention time Charges PEP MS/MS scan number Raw file Score Delta score Intensity Intensity 2 Intensity 3 Reverse Potential contaminant id Protein group IDs Peptide ID Evidence IDs MS/MS IDs Best MS/MS GFP-Chromophore site IDs Pyl->PocK site IDs Pyl->TCOK site IDs MS/MS Count AAGITHGMDELYK Unmodified 1404.6708 0.6707503 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 25.25 25.384 3 0.040011 5843 20190218_JEBM_GFP3 13.404 13.404 253560 0 253560 0 0;1 0 0;1 0;1 1 2 DHMVLLEFVTA Unmodified 1273.6377 0.63765925 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS 2 34.828 34.962 2 4.8784E-14 11911 20190218_JEBM_GFP3 60.973 60.973 0 0 0 1 0;1 1 2;3 2;3 3 2 DHMVLLEFVTAA Unmodified 1344.6748 0.67477304 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 35.215 35.282 2 1.2613E-11 12091 20190218_JEBM_GFP3 58.676 58.676 3430300 488500 2941800 2 0;1 2 4;5 4;5;6;7 7 4 DHMVLLEFVTAAGIT Unmodified 1615.828 0.82797922 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 36.734 36.801 2 1.6788E-15 13064 20190218_JEBM_GFP3 87.298 87.298 671370 281070 390300 3 0;1 3 6;7 8;9;10 9 3 DHMVLLEFVTAAGITH Unmodified 1752.8869 0.88689108 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 2 2 33.83 33.897 2;3 2.359E-85 10834 20190218_JEBM_GFP2 178.71 178.71 10179000 8690000 1488700 4 0;1 4 8;9;10;11 11;12;13;14;15;16 12 6 DHMVLLEFVTAAGITHGMDELY Unmodified 2461.1658 0.16576806 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 36.148 36.216 3 0.0081592 12735 20190218_JEBM_GFP3 29.136 29.136 643200 201550 441650 5 0;1 5 12;13 17 17 1 DHMVLLEFVTAAGITHGMDELYK Unmodified 2589.2607 0.26073108 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 7 8 36.807 36.859 3;4 5.0356E-114 11321 20190218_JEBM_GFP3 151.82 151.82 435400000 245270000 190130000 6 0;1 6 14;15;16;17;18;19;20;21;22;23;24;25;26;27;28 18;19;20;21;22;23;24;25;26;27;28;29;30;31;32;33;34;35 28 15 DHMVLLEFVTAAGITHGMDELYKG Unmodified 2646.2822 0.2821948 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By MS/MS 2 2 33.913 33.98 3;4 0.00055628 10902 20190218_JEBM_GFP2 50.425 50.425 6594700 4848000 1746700 7 0;1 7 29;30;31;32 36;37;38;39 36 3 EDGNILGHK Unmodified 981.48796 0.48795855 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS 1 21.137 21.137 2 0.053191 3268 20190218_JEBM_GFP2 16.144 16.144 249220 249220 0 8 0;1 8 33 40;41 40 2 EFVTAAGITHGMDELYK Unmodified 1880.8978 0.8978497 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 29.398 29.465 2 1.2242E-70 8449 20190218_JEBM_GFP3 147.96 147.96 1292900 402230 890620 9 0;1 9 34;35 42 42 1 FEGDTLVN Unmodified 893.41306 0.41306227 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By matching 1 1 28.473 28.54 1 0.0043023 7372 20190218_JEBM_GFP2 33.738 33.738 1537300 1408300 129040 10 0;1 10 36;37 43 43 1 FEGDTLVNR Unmodified 1049.5142 0.5141733 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 20 18 33.465 33.519 2 3.514E-151 5522 20190218_JEBM_GFP2 190.73 190.73 3518200000 2390700000 1127500000 11 0;1 11 38;39;40;41;42;43;44;45;46;47;48;49;50;51;52;53;54;55;56;57;58;59;60;61;62;63;64;65;66;67;68;69;70;71;72;73;74;75 44;45;46;47;48;49;50;51;52;53;54;55;56;57;58;59;60;61;62;63;64;65;66;67;68;69;70;71;72;73;74;75;76;77;78;79;80;81;82;83;84;85;86;87;88;89;90;91;92;93;94;95;96;97;98;99;100 44 52 FEGDTLVNRIELK Unmodified 1532.8199 0.81985737 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By matching 1 1 27.841 27.908 3 0.0017462 7038 20190218_JEBM_GFP2 52.693 52.693 577660 502110 75552 12 0;1 12 76;77 101;102 101 2 FNSHOVYITADK Pyl->PocK 1503.7358 0.73579339 1 GFP_150TAG yes yes 0 1 0 0 By MS/MS 2 25.884 25.884 2;3 1.655E-23 5746 20190218_JEBM_GFP2 93.348 90.986 2608300 2608300 0 13 1 13 78;79 103;104;105 103 1 3 GEELFTGVVPILVELD Unmodified 1728.9186 0.91856837 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 39.289 39.306 2 3.013E-32 14636 20190218_JEBM_GFP3 113.63 111.63 858140 191750 666390 14 0;1 14 80;81 106 106 1 GEELFTGVVPILVELDGD Unmodified 1900.967 0.96697512 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 39.517 39.534 2 5.0447E-71 14775 20190218_JEBM_GFP3 151.1 151.1 903370 155050 748320 15 0;1 15 82;83 107;108 108 2 GEELFTGVVPILVELDGDVN Unmodified 2114.0783 0.078316486 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By matching 1 1 39.382 39.4 3 0.04984 14263 20190218_JEBM_GFP2 30.684 30.684 326950 211330 115620 16 0;1 16 84;85 109 109 1 GIDFKEDGNILGHK Unmodified 1541.7838 0.78380622 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By matching 1 1 25.6 25.667 3 0.011659 5565 20190218_JEBM_GFP2 26.124 26.124 1169900 539260 630660 17 0;1 17 86;87 110 110 1 GPVLLPDNHYLSTQSVLSK Unmodified 2067.1001 0.10005498 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 28.413 28.48 3 7.8451E-06 7822 20190218_JEBM_GFP3 39.657 39.657 4730000 3039700 1690200 18 0;1 18 88;89 111;112;113 113 3 HMVLLEFVTAAGITHGMDELYK Unmodified 2474.2338 0.23378805 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS 1 32.846 32.846 3 2.5295E-10 10243 20190218_JEBM_GFP2 73.735 73.735 290160 290160 0 19 0;1 19 90 114 114 1 HNVEDGSVQLADHYQQ Unmodified 1838.8184 0.81835383 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 24.337 24.404 3 7.5616E-23 4819 20190218_JEBM_GFP2 100.11 100.11 3943500 2357400 1586100 20 0;1 20 91;92 115;116;117;118 116 4 HNVEDGSVQLADHYQQN Unmodified 1952.8613 0.86128127 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 2 2 24.227 24.294 2;3 1.2089E-43 4758 20190218_JEBM_GFP2 127.78 127.78 18108000 9760800 8347000 21 0;1 21 93;94;95;96 119;120 119 2 HYLSTQSVLSK Unmodified 1261.6667 0.6666512 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By matching 1 1 23.597 23.664 3 0.0024552 4387 20190218_JEBM_GFP2 46.796 46.796 272810 163150 109660 22 0;1 22 97;98 121 121 1 IDFKEDGNILGHK 2 GFP-Chromophore 1444.7099 0.709913 1;0 GFP_102TAG;GFP_150TAG no no 2 0 0 1 By MS/MS By matching 1 1 23.931 23.998 3 0.061435 4587 20190218_JEBM_GFP2 25.98 21.917 1568400 921970 646440 23 0;1 23 99;100 122 122 1 IRHNVEDGSVQLADHYQQN Unmodified 2222.0465 0.046456281 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By MS/MS 1 1 23.335 23.403 4 0.026715 4208 20190218_JEBM_GFP2 36.593 36.593 1903300 1007400 895860 24 0;1 24 101;102 123;124 123 2 KLEYNFNSHLVYITADKQKNGIK 3 GFP-Chromophore 2662.3656 0.36560558 0 GFP_102TAG yes yes 3 0 0 3 By MS/MS By matching 1 1 32.503 32.57 3 0.052843 10086 20190218_JEBM_GFP2 27.035 27.035 10935000 5880800 5054000 25 0 25 103;104 125 125 8 1 KNGIKANFKIR GFP-Chromophore 1267.7513 0.7513243 1;0 GFP_102TAG;GFP_150TAG no no 1 0 0 3 By MS/MS 1 29.416 29.551 2 0.05906 8544 20190218_JEBM_GFP3 0 0 63525 0 63525 26 0;1 26 105 126 126 0 LEYNFNSH Unmodified 1022.4458 0.44575938 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 2 26.066 26.155 2 4.2782E-59 5754 20190218_JEBM_GFP2 141.52 141.52 85843000 57825000 28019000 27 0;1 27 106;107;108 127;128;129 128 2 LEYNFNSHLVYITADK Unmodified 1925.9523 0.95232811 0 GFP_102TAG yes yes 0 0 0 0 By MS/MS By matching 4 1 32.856 32.883 3 0.011567 11049 20190218_JEBM_GFP2 40.941 38.198 11220000 1232000 9988000 28 0 28 109;110;111;112;113 130;131;132;133 130 3 LEYNFNSHOVY Pyl->TCOK 1564.7562 0.75619449 1 GFP_150TAG yes yes 0 0 1 0 By MS/MS 2 33.636 33.77 2 1.3964E-25 11028 20190218_JEBM_GFP3 83.182 83.182 23405000 0 23405000 29 1 29 114;115 134;135 134 1 1 LEYNFNSHOVY Pyl->PocK;GFP-Chromophore 1474.6517 0.65172941 1 GFP_150TAG yes yes 1 1 0 0 By matching By MS/MS 1 1 39.341 39.358 2 0.051577 14653 20190218_JEBM_GFP3 4.4511 4.4511 2102200 1149600 952580 30 1 29 116;117 136 136 3;10 1 0 LEYNFNSHOVYIT Pyl->PocK 1708.8097 0.80968662 1 GFP_150TAG yes yes 0 1 0 0 By MS/MS 1 30.825 30.825 2 0.025143 8937 20190218_JEBM_GFP2 36.318 36.318 819160 819160 0 31 1 30 118 137 137 1 1 LEYNFNSHOVYITADK Pyl->PocK 2022.9687 0.96870646 1 GFP_150TAG yes yes 0 1 0 0 By MS/MS By MS/MS 8 2 36.159 36.193 3 1.822E-60 7792 20190218_JEBM_GFP2 135.47 135.47 4850000 4685200 164790 32 1 31 119;120;121;122;123;124;125;126;127;128 138;139;140;141;142;143;144;145;146 140 1 7 LEYNFNSHOVYITADK Pyl->TCOK 2093.047 0.046956778 1 GFP_150TAG yes yes 0 0 1 0 By MS/MS 1 31.469 31.604 3 1.9713E-35 9985 20190218_JEBM_GFP3 89.483 89.483 922560000 0 922560000 33 1 31 129 147;148 148 1 2 LPVPWPTLV Unmodified 1020.6008 0.60080346 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS 1 37.007 37.142 2 0.039571 13295 20190218_JEBM_GFP3 20.699 20.699 79351 0 79351 34 0;1 32 130 149 149 1 LPVPWPTLVTTLTYGVQCFSR GFP-Chromophore 2414.2457 0.24567336 1;0 GFP_102TAG;GFP_150TAG no no 1 0 0 0 By MS/MS By matching 1 1 36.429 36.496 3 7.4003E-06 12597 20190218_JEBM_GFP2 63.447 63.447 166960000 126240000 40715000 35 0;1 33 131;132 150;151;152;153;154;155 153 0 6 LTLKFICTTGK 4 GFP-Chromophore 1200.6114 0.61138492 1;0 GFP_102TAG;GFP_150TAG no no 4 0 0 1 By matching By MS/MS 1 1 25.074 25.141 2 0.05906 5680 20190218_JEBM_GFP3 0 0 459080 128020 331060 36 0;1 34 133;134 156 156 0 MVLLEFVTAAGITHGMDELYK Unmodified 2337.1749 0.17487618 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 34.406 34.473 3 7.196E-34 11623 20190218_JEBM_GFP3 116.35 116.35 3808700 2845900 962830 37 0;1 35 135;136 157;158 157 2 NFNSHOVYITADK Pyl->TCOK 1687.857 0.85697116 1 GFP_150TAG yes yes 0 0 1 0 By MS/MS 1 29.698 29.832 3 0.020057 8686 20190218_JEBM_GFP3 20.65 20.65 629760 0 629760 38 1 36 137 159 159 1 1 NHYLSTQSVLSK Unmodified 1375.7096 0.70957864 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS 1 23.916 23.916 2 1.3703E-06 4545 20190218_JEBM_GFP2 52.79 52.79 0 0 0 39 0;1 37 138 160 160 1 NSHOVYITADK Pyl->TCOK;3 GFP-Chromophore 1366.667 0.66698555 1 GFP_150TAG yes yes 3 0 1 0 By MS/MS By MS/MS 2 1 35.02 35.087 2 0.04411 13561 20190218_JEBM_GFP2 12.213 1.0007 885510 427680 457840 40 1 38 139;140;141 161;162 161 3;7;10 1 1 NSHOVYITADKQK 3 GFP-Chromophore 1579.7896 0.7895603 1 GFP_150TAG yes yes 3 0 0 1 By MS/MS 1 28.651 28.786 2 0.0017026 8026 20190218_JEBM_GFP3 37.556 37.556 0 0 0 41 1 39 142 163 163 3;7;10 1 NTPIGDGPVLLPDNHYLSTQSVLSK Unmodified 2664.3759 0.37589549 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 29.614 29.681 3 6.2191E-07 8591 20190218_JEBM_GFP3 63.522 63.522 12238000 7243400 4994100 42 0;1 40 143;144 164;165 164 2 OVYITADK Pyl->PocK 1018.5335 0.53351176 1 GFP_150TAG yes yes 0 1 0 0 By MS/MS 1 26.09 26.09 2 1.6878E-60 5865 20190218_JEBM_GFP2 139.97 139.97 82534000 82534000 0 43 1 41 145 166;167;168;169 166 1 4 OVYITADKQK Pyl->TCOK;2 GFP-Chromophore 1304.7129 0.71287311 1 GFP_150TAG yes yes 2 0 1 1 By MS/MS 1 31.077 31.212 2 0.063905 9562 20190218_JEBM_GFP3 0.78953 0.78953 46170 0 46170 44 1 42 146 170 170 3;7 1 0 PDNHYLSTQSVLSK Unmodified 1587.7893 0.78928553 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By matching 1 1 24.953 25.021 3 0.06401 5237 20190218_JEBM_GFP2 26.232 26.232 457600 201520 256070 45 0;1 43 147;148 171 171 1 RDHMVLLEF Unmodified 1158.5856 0.5855641 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By MS/MS 2 2 29.496 29.563 2;3 0.00051295 8102 20190218_JEBM_GFP2 31.934 31.934 1541300 1101700 439590 46 0;1 44 149;150;151;152 172;173;174 172 3 RDHMVLLEFVTA Unmodified 1429.7388 0.73877028 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By matching By MS/MS 1 2 33.525 33.582 2 0.031868 14254 20190218_JEBM_GFP3 35.944 35.944 777540 202570 574970 47 0;1 45 153;154;155 175;176 176 2 RDHMVLLEFVTAA Unmodified 1500.7759 0.77588407 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By MS/MS 2 2 31.482 31.55 2;3 2.1234E-23 9760 20190218_JEBM_GFP3 67.08 67.08 9053400 559420 8494000 48 0;1 46 156;157;158;159 177;178;179;180;181;182 181 6 RDHMVLLEFVTAAGITH Unmodified 1908.988 0.98800211 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS 1 31.103 31.103 4 1.3018E-25 9081 20190218_JEBM_GFP2 74.786 74.786 6048300 6048300 0 49 0;1 47 160 183;184 184 2 RDHMVLLEFVTAAGITHGMDELYK Unmodified 2745.3618 0.36184211 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 1 By MS/MS By MS/MS 10 9 34.123 34.176 3;4;5 1.5795E-165 9736 20190218_JEBM_GFP2 215.39 215.39 1183000000 787370000 395650000 50 0;1 48 161;162;163;164;165;166;167;168;169;170;171;172;173;174;175;176;177;178;179 185;186;187;188;189;190;191;192;193;194;195;196;197;198;199;200;201;202;203;204;205;206;207;208;209;210;211;212;213;214;215;216;217;218;219;220 190 35 RDHMVLLEFVTAAGITHGMDELYKG Unmodified 2802.3833 0.38330583 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 2 By MS/MS By MS/MS 3 3 30.167 30.234 4;5 1.4465E-06 10173 20190218_JEBM_GFP3 40.404 40.404 19500000 14717000 4782700 51 0;1 49 180;181;182;183;184;185 221;222;223;224;225;226;227;228 228 8 SAMPEGYVQER Unmodified 1265.571 0.57103625 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 13 9 33.987 34.028 2 4.5192E-91 5101 20190218_JEBM_GFP2 143.03 143.03 1629000000 900810000 728190000 52 0;1 50 186;187;188;189;190;191;192;193;194;195;196;197;198;199;200;201;202;203;204;205;206;207 229;230;231;232;233;234;235;236;237;238;239;240;241;242;243;244;245;246;247;248;249;250;251;252;253;254;255;256;257;258 232 27 SFODDGTYK Pyl->TCOK;2 GFP-Chromophore 1171.5186 0.51858998 0 GFP_102TAG yes yes 2 0 1 0 By MS/MS By matching 1 1 32.15 32.217 2 0.049935 9815 20190218_JEBM_GFP2 16.54 16.54 2816200 441270 2374900 53 0 51 208;209 259 259 2;4;9 0 1 SHOVYITADK Pyl->PocK 1242.6245 0.62445203 1 GFP_150TAG yes yes 0 1 0 0 By MS/MS 2 24.023 24.023 2;3 0.011589 4623 20190218_JEBM_GFP2 20.329 20.329 428700 428700 0 54 1 52 210;211 260;261 260 1 2 TGVVPILVELDGDVNGHK Unmodified 1860.9945 0.99452728 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 31.285 31.352 3 0.027475 9704 20190218_JEBM_GFP3 36.193 36.193 1029900 818610 211250 55 0;1 53 212;213 262 262 1 TISFODDGTYKTRAEV Pyl->TCOK;3 GFP-Chromophore 1921.921 0.92102798 0 GFP_102TAG yes yes 3 0 1 2 By matching By MS/MS 1 1 30.389 30.456 3 0.060095 9085 20190218_JEBM_GFP3 16.139 9.9757 420440 256900 163540 56 0 54 214;215 263 263 2;4;5;9 0 0 TISFODDGTYKTRAEVKFEGDT Unmodified 2616.2708 0.27076172 0 GFP_102TAG yes yes 0 0 0 3 By MS/MS By matching 1 1 30.66 30.727 3 0.072429 8891 20190218_JEBM_GFP2 24.227 24.227 3054500 1504100 1550400 57 0 55 216;217 264 264 1 TISFODDGTYKTRAEVKFEGDTLV Pyl->PocK;3 GFP-Chromophore 2741.2973 0.29731082 0 GFP_102TAG yes yes 3 1 0 3 By MS/MS By matching 1 1 29.421 29.488 3 0.033328 8050 20190218_JEBM_GFP2 29.842 29.842 82026000 62493000 19533000 58 0 56 218;219 265 265 1;2;4;5;6;9 0 1 TPIGDGPVLLPDNHYLSTQSVLSK Unmodified 2550.333 0.33296804 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By MS/MS 1 1 29.583 29.65 3 3.9682E-46 8538 20190218_JEBM_GFP3 124.86 124.86 52673000 36422000 16251000 59 0;1 57 220;221 266;267;268 267 3 VTAAGITHGMDELYK Unmodified 1604.7868 0.78684269 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By matching By MS/MS 1 1 26.838 26.906 3 1.2055E-05 6865 20190218_JEBM_GFP3 56.139 56.139 9492100 5831800 3660300 60 0;1 58 222;223 269;270 269 2 YLSTQSVLSK Unmodified 1124.6077 0.60773933 1;0 GFP_102TAG;GFP_150TAG no no 0 0 0 0 By MS/MS By matching 1 1 25.419 25.486 2 1.8778E-34 5454 20190218_JEBM_GFP2 118.03 118.03 3409500 2504100 905360 61 0;1 59 224;225 271;272 272 2