Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param 20190218_JEBM_GFP2 2 Trypsin/P Semispecific Trypsin/P Specific True Pyl->PocK;Pyl->TCOK;GFP-Chromophore Carbamidomethyl (C) False True 1 0 Standard 4121 11623 0 14505 8741 0 5764 158 133 0 25 1.09 1.52 NaN 0.43 43 143973 6047 4.2 3747 61.96 15087 4335 4208 28.73 35.29 2946 67.96 + 0.76834 0.90008 0.50132 0.59871 20190218_JEBM_GFP3 3 Trypsin/P Semispecific Trypsin/P Specific True Pyl->PocK;Pyl->TCOK;GFP-Chromophore Carbamidomethyl (C) False True 1 0 Standard 4007 12140 0 14607 9673 0 4934 91 79 0 12 0.62 0.82 NaN 0.24 38 160602 6733 4.19 3864 57.39 18030 5111 4992 28.35 35.46 3173 62.08 + 0.86923 1.0372 0.61338 0.76251 Total 8128 23763 0 29112 18414 0 10698 249 212 0 37 0.86 1.15 NaN 0.35 60 304575 33117 9446 9200 28.52 35.39 6119 64.78 0.80661 0.95442 0.54383 0.6656