Time course treatment with 0.4 mM Palmitate and 200 nM thapsigargin of insulinoma INS-1E cells. Timepoints; 0, 4, 16 and 24h in biological duplicates within an iTRAQ 8 set for palmitate and thapsigargin, respectively. 2020-08-18 Henrik Johansson Karolinska Institutet Janne Lehtiö Palmitate, thapsigargin, insulinoma, diabetes 100702_VR_Palm_fr_11.raw raw 453879808 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_12.raw raw 424600814 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_13.raw raw 475553449 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_14.raw raw 492987497 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_15.raw raw 445700502 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_16.raw raw 353829086 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_17.raw raw 348133039 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_18.raw raw 352385299 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_19.raw raw 389505480 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_20.raw raw 404362647 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_21.raw raw 347474917 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_60min_Fr_22.raw raw 352209602 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_90min_fr_17.raw raw 504787405 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_90min_fr_23.raw raw 636042178 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_90min_fr_24.raw raw 516542419 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_90min_fr_25.raw raw 555670206 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100709_VR_Palm_90min_fr_26.raw raw 512268255 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_32.raw raw 391960344 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_33.raw raw 386083316 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_34.raw raw 350011778 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_35.raw raw 358448256 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_36.raw raw 395306850 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_37.raw raw 358005204 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_38.raw raw 368880885 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_39.raw raw 350060133 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_40.raw raw 366217895 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_41.raw raw 368007177 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_42.raw raw 384991681 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_43.raw raw 357098043 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_60.raw raw 379546363 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_61.raw raw 423081246 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_62.raw raw 380919077 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_63.raw raw 399926851 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_64.raw raw 447054912 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_65.raw raw 377724245 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_66.raw raw 392779784 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_67.raw raw 418190542 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_68.raw raw 445391384 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_60min_fr_69.raw raw 402437833 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_28.raw raw 511217029 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_29.raw raw 527244451 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_30.raw raw 519947441 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_31.raw raw 452282916 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_44.raw raw 488909805 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_45.raw raw 526425145 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_46.raw raw 441975385 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_47.raw raw 465883219 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_48.raw raw 454499791 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_49.raw raw 464160162 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_50.raw raw 509045929 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_51.raw raw 452918315 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_52.raw raw 471484689 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_53.raw raw 470614468 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_54.raw raw 481326089 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_55.raw raw 484748695 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_56.raw raw 504524975 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_57.raw raw 507480660 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_58.raw raw 514223194 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100710_VR_Palm_90min_fr_59.raw raw 504419783 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_25.raw raw 665118019 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_26.raw raw 550692389 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_27.raw raw 559989979 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_28.raw raw 527605913 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_29.raw raw 540565680 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_30.raw raw 547326405 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_31.raw raw 562622963 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_32.raw raw 563356173 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_33.raw raw 587999230 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_35.raw raw 600996775 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_36.raw raw 523138661 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_37.raw raw 567783061 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_38.raw raw 546882784 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100819_VR_Palm_60min_fr_39.raw raw 563297372 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_4.raw raw 567183735 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_5.raw raw 454546816 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_6.raw raw 229421899 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_7.raw raw 402746760 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_8.raw raw 495034255 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_9.raw raw 437276575 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100702_VR_Palm_fr_10.raw raw 327968471 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_04.raw raw 836477022 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_05.raw raw 579442223 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_06.raw raw 516989325 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_07.raw raw 496495826 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_08.raw raw 471123909 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_09.raw raw 404484144 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_10.raw raw 469284151 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_11.raw raw 395690380 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_12.raw raw 366846314 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_13.raw raw 313556853 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_14.raw raw 296577678 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_15.raw raw 283263677 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_16.raw raw 299745529 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_17.raw raw 279337272 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_18.raw raw 279839557 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_19.raw raw 292532953 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_20.raw raw 296282877 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_21.raw raw 321406809 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_22.raw raw 372635719 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_23.raw raw 404937075 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_24.raw raw 404185641 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_25.raw raw 418905015 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_26.raw raw 383141077 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_27.raw raw 352021577 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_28_1st_after_cal.raw raw 522314104 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_29.raw raw 393527428 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_30.raw raw 355966903 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_31.raw raw 364276377 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_32.raw raw 353600183 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_33.raw raw 336645632 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_34.raw raw 327029813 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_35.raw raw 348754574 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_36.raw raw 354102189 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_37.raw raw 343042059 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_38.raw raw 337736526 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_39.raw raw 351987726 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_40.raw raw 340503030 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_40_2.raw raw 361885642 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_41.raw raw 355311351 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_42.raw raw 350054270 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_43.raw raw 361556983 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_44.raw raw 335585233 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_45.raw raw 333652640 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_46.raw raw 320741163 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_47.raw raw 323229849 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_48.raw raw 326819340 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_49.raw raw 336554200 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_50.raw raw 337599773 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_51.raw raw 330812557 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_52.raw raw 329612365 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_53.raw raw 342498749 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_54.raw raw 327919772 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_55.raw raw 440526862 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_56.raw raw 505105004 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_57.raw raw 504858172 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_58.raw raw 520798124 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_59.raw raw 463735518 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_60.raw raw 404510997 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_61.raw raw 715178210 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_62.raw raw 427827135 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_63.raw raw 423274213 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_64.raw raw 475485913 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_65.raw raw 485625701 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_66.raw raw 472691318 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_67.raw raw 460946123 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_68.raw raw 472312711 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_69.raw raw 444926153 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr25.raw raw 710417642 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr26.raw raw 523920435 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr27.raw raw 564675485 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr28.raw raw 540546741 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr29.raw raw 511641747 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr30.raw raw 510382385 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr31.raw raw 537322027 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr32.raw raw 562453337 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr33.raw raw 549965548 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr34.raw raw 557870204 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr35.raw raw 549865987 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr36.raw raw 558890014 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr37.raw raw 555082844 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr38.raw raw 552997220 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr39.raw raw 548256928 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100818_VR_Thaps_60min_fr40.raw raw 545769725 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100618_VR_Thaps_IPG34to48_fr_07.raw raw 820086404 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100618_VR_Thaps_IPG34to48_fr_09.raw raw 759870407 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100618_VR_Thaps_IPG34to48_fr_10.raw raw 666136951 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100618_VR_Thaps_IPG34to48_fr_11.raw raw 666341783 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100618_VR_Thaps_IPG34to48_fr_12.raw raw 982573524 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_01.raw raw 600810512 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_02.raw raw 575925633 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100624_VR_Thaps_fr_03.raw raw 846010143 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole IPG-IEF pH 3.4-4.8 fractionation The iTRAQ labelled peptides were separated by immobilized pH gradient - isoelectric focusing (IPG-IEF) on a 3.4-4.8 strip. Peptides were extracted from the strips into 72 fractions by a prototype liquid handling robot, supplied by GE Healthcare Bio-Sciences AB. A plastic device with 72 wells was put onto each strip and 50 µl of ultra-pure water (Milli-Q, Millipore) was added to each well. After 30 minutes incubation, the liquid was transferred to a 96 well plate and the extraction was repeated 2 more times. The extracted peptides were dried in a SpeedVac vaccum concentrator and dissolved in 3% acetronitrile (ACN), 0.1 % formic acid. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100514_VR_Palm_4h_2microg.raw raw 1557459337 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100514_VR_Palm_4h_from5_2microg.raw raw 1745956210 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100426_VR_Palm_4h_2microg.raw raw 1612798577 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100514_VR_Thaps_4h_2microg.raw raw 1537938569 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100514_VR_Thaps_4h_from5_2microg.raw raw 1658923515 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 100426_VR_Thaps_4h_2microg.raw raw 1597002638 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 113 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 114 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 115 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 116 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 117 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 118 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 119 Rattus rattus (Black rat) cell culture insulinoma diabetes Treatment with palmitate or thapsigargin for 0, 4, 16 and 24 hours in duplicates. Whole 240 min RP gradient The TMT labelled peptides were separated by a 240 min gradient before analysis by MS. Trypsin Carbamidomethyl (C) Oxidation (M) Rattus rattus (Black rat) iTRAQ8plex (K) iTRAQ8plex (N-term) LTQ Orbitrap Velos DDA-high res. Relative quantification Product ion (reporter quantification) 8 See excel file with protein search results for sample assignments to each iTRAQ tag. iTRAQ 121 VR_Thaps_IPG-IEF_fr1-69_3x4h_uniprot_rat.msf result 8252215296 Proteome Discoverer 1.3 VR_Palm_IPG-IEF_fr1-69_3x4h_uniprot_rat.msf result 9786109952 Proteome Discoverer 1.3 VR_Palm_n_Thaps_2xIPG-IEF_fr1-69_3x4h_uniprot_rat.pep.xml result 518791202 Proteome Discoverer 1.3 VR_Palm_n_Thaps_2xIPG-IEF_fr1-69_3x4h_uniprot_rat.prot.xml result 173309047 Proteome Discoverer 1.3 Proteomics_Thaps_n_Palm.xlsx result 5181801 other Proteome Discoverer 1.3