Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param 190506_IP_mESC_NE_1_control_03 1_control Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6091 57216 0 60107 54325 0 5782 30159 29623 0 536 50.18 54.53 NaN 9.27 15314 1074329 36583 3.41 12293 33.6 169685 31915 29532 18.81 22.43 12952 40.58 + 0.61991 0.90494 0.41301 0.62806 190506_IP_mESC_NE_2_control_01 2_control Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6000 57502 0 60861 54143 0 6718 30782 30162 0 620 50.58 55.71 NaN 9.23 15389 1044604 35828 3.43 12704 35.46 164062 31215 28877 19.03 22.81 13050 41.81 + 0.63023 0.92305 0.41613 0.63551 190506_IP_mESC_NE_2_control_02 2_control Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6028 57346 0 60778 53914 0 6864 30811 30223 0 588 50.69 56.06 NaN 8.57 15316 1028274 35952 3.5 12548 34.9 162699 31216 28984 19.19 22.89 12926 41.41 + 0.63131 0.91862 0.41654 0.63101 190506_IP_mESC_NE_2_control_03 2_control Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6103 57040 0 60597 53483 0 7114 30645 30057 0 588 50.57 56.2 NaN 8.27 15138 1018343 35840 3.52 12410 34.63 160279 30915 28779 19.29 22.86 12944 41.87 + 0.62518 0.91085 0.41089 0.62163 190506_IP_mESC_NE_4_Klf2_01 4_Klf2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6032 57787 0 60604 54970 0 5634 26424 26027 0 397 43.6 47.35 NaN 7.05 13587 1036785 36196 3.49 12289 33.95 162602 31388 29254 19.3 22.18 12888 41.06 + 0.63501 0.91813 0.42929 0.64847 190506_IP_mESC_NE_4_Klf2_02 4_Klf2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6042 57782 0 60621 54943 0 5678 26279 25807 0 472 43.35 46.97 NaN 8.31 13730 1043943 36793 3.52 12153 33.03 165315 31999 29997 19.36 21.94 12807 40.02 + 0.63317 0.91334 0.43352 0.65776 190506_IP_mESC_NE_4_Klf2_03 4_Klf2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6154 57452 0 60228 54676 0 5552 26340 25908 0 432 43.73 47.38 NaN 7.78 13688 1038442 36314 3.5 12262 33.77 161934 31463 29483 19.43 22.16 12921 41.07 + 0.62829 0.90905 0.4298 0.6576 190514_IP_mESC_NE_1_control_01 1_control Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6026 57106 0 60335 53877 0 6458 29198 28678 0 520 48.39 53.23 NaN 8.05 14903 1056641 36076 3.41 12451 34.51 166521 31554 29194 18.95 22.78 13022 41.27 + 0.62334 0.90824 0.42082 0.6401 190514_IP_mESC_NE_1_control_02 1_control Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6061 57110 0 60736 53484 0 7252 29084 28572 0 512 47.89 53.42 NaN 7.06 14908 1028566 35899 3.49 12446 34.67 163966 31501 29296 19.21 23.08 12998 41.26 + 0.62047 0.90225 0.41764 0.63335 190514_IP_mESC_NE_3_Klf2_01 3_Klf2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6144 57175 0 60038 54312 0 5726 23032 22694 0 338 38.36 41.78 NaN 5.9 11689 1069289 36251 3.39 12183 33.61 168396 31565 29241 18.74 21.8 12718 40.29 + 0.64717 0.92854 0.4414 0.66282 190514_IP_mESC_NE_3_Klf2_02 3_Klf2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6004 57868 0 60916 54820 0 6096 22996 22623 0 373 37.75 41.27 NaN 6.12 11745 1065775 36236 3.4 12337 34.05 168796 31497 29202 18.66 21.79 12810 40.67 + 0.65175 0.9361 0.44444 0.66378 190514_IP_mESC_NE_3_Klf2_03 3_Klf2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Carbamyl (N-term) False False 1 2 Standard 6124 57297 0 60350 54244 0 6106 23193 22869 0 324 38.43 42.16 NaN 5.31 11804 1058043 36060 3.41 12235 33.93 167452 31385 29218 18.74 21.96 12825 40.86 + 0.65664 0.94034 0.45138 0.67687 1_control 19614 2_control 19279 3_Klf2 14682 4_Klf2 16978 Total 72809 688681 0 726171 651191 0 74980 328943 323243 0 5700 45.3 49.64 NaN 7.6 28486 12563034 1981707 377613 351057 19.05 22.38 154861 41.01 0.63253 0.91703 0.42584 0.64509