Mascot Search Results User : Email : Search title : MS data file : F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data¥PMFtrim¥trim+LS2NS005 B1_0001.mgf Database : NCBInr 20040815 (1986685 sequences; 666719865 residues) Taxonomy : Mus. (74538 sequences) Timestamp : 28 Sep 2004 at 10:53:10 GMT Top Score : 95 for gi¦33585712, Car6 protein [Mus musculus] Probability Based Mowse Score Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 61 are significant (p<0.05). Concise Protein Summary Report Switch to full Protein Summary Report To create a bookmark for this report, right click this link: Concise Summary Report (../data/20040928/ F032077.dat) 1. gi¦33585712 Mass: 36555 Score: 95 Expect: 2.3e-005 Peptides matched: 10 Car6 protein [Mus musculus] gi¦29612611 Mass: 37325 Score: 94 Expect: 2.9e-005 Peptides matched: 10 Car6 protein [Mus musculus] gi¦28461317 Mass: 29221 Score: 77 Expect: 0.0016 Peptides matched: 8 Car6 protein [Mus musculus] 2. gi¦5921196 Mass: 36440 Score: 81 Expect: 0.00065 Peptides matched: 9 Carbonic anhydrase VI precursor (Carbonate dehydratase VI) (CA-VI) (Secreted carbonic anhydrase) (S gi¦3421369 Mass: 30239 Score: 61 Expect: 0.054 Peptides matched: 7 stress-inducible intra-cellular carbonic anhydrase isozyme VI [Mus musculus] 3. gi¦90689 Mass: 11778 Score: 44 Expect: 3.2 Peptides matched: 4 Ig heavy chain precursor V region (BFL23) - mouse (fragment) gi¦1334038 Mass: 13001 Score: 42 Expect: 5 Peptides matched: 4 unnamed protein product [Mus musculus] 4. gi¦12861258 Mass: 18421 Score: 38 Expect: 11 Peptides matched: 4 unnamed protein product [Mus musculus] gi¦12860679 Mass: 19673 Score: 37 Expect: 14 Peptides matched: 4 unnamed protein product [Mus musculus] 5. gi¦26331700 Mass: 62029 Score: 37 Expect: 14 Peptides matched: 6 unnamed protein product [Mus musculus] gi¦12858423 Mass: 63029 Score: 37 Expect: 15 Peptides matched: 6 unnamed protein product [Mus musculus] gi¦11321424 Mass: 65849 Score: 36 Expect: 18 Peptides matched: 6 Ral-A exchange factor RalGPS2 [Mus musculus] gi¦31560036 Mass: 65845 Score: 36 Expect: 18 Peptides matched: 6 Ral-A exchange factor RalGPS2 [Mus musculus] gi¦28570774 Mass: 3435 Score: 28 Expect: 1.1e+002 Peptides matched: 2 immunoglobulin heavy chain [Mus musculus] 6. gi¦13508539 Mass: 67012 Score: 37 Expect: 16 Peptides matched: 7 CLASP2 [Mus musculus] 7. gi¦1166506 Mass: 83772 Score: 37 Expect: 16 Peptides matched: 7 Stat3B gi¦17512414 Mass: 83758 Score: 37 Expect: 16 Peptides matched: 7 Signal transducer and activator of transcription 3, isoform 3 [Mus musculus] gi¦458706 Mass: 88809 Score: 35 Expect: 24 Peptides matched: 7 Stat3 gi¦34559410 Mass: 88796 Score: 35 Expect: 24 Peptides matched: 7 signal transducer and activator of transcription 3 [Mus musculus] gi¦2137460 Mass: 88778 Score: 35 Expect: 25 Peptides matched: 7 ISGF3 p91-related transcription factor - mouse 8. gi¦2137361 Mass: 35986 Score: 36 Expect: 18 Peptides matched: 5 GPI-anchored protein - mouse (fragment) 9. gi¦13529410 Mass: 41732 Score: 35 Expect: 24 Peptides matched: 6 Nf2 protein [Mus musculus] 10. gi¦2598562 Mass: 72547 Score: 34 Expect: 27 Peptides matched: 7 BiP [Mus musculus] 11. gi¦41946803 Mass: 32879 Score: 34 Expect: 28 Peptides matched: 5 RIKEN cDNA 2900024D24 [Mus musculus] 12. gi¦409226 Mass: 275164 Score: 34 Expect: 32 Peptides matched: 13 brain beta spectrin [Mus musculus] gi¦448251 Mass: 272627 Score: 34 Expect: 32 Peptides matched: 13 beta spectrin (beta fodrin) gi¦19353700 Mass: 14756 Score: 32 Expect: 45 Peptides matched: 4 Unknown (protein for IMAGE:5361724) [Mus musculus] gi¦38088507 Mass: 23166 Score: 31 Expect: 55 Peptides matched: 4 similar to Spindlin-like protein 2 (SPIN-2) [Mus musculus] 13. gi¦37359958 Mass: 73622 Score: 34 Expect: 32 Peptides matched: 6 mKIAA0450 protein [Mus musculus] 14. gi¦18606452 Mass: 50742 Score: 33 Expect: 37 Peptides matched: 5 Expressed sequence C80913 [Mus musculus] 15. gi¦1173483 Mass: 59817 Score: 33 Expect: 38 Peptides matched: 5 receptor tyrosine kinase 16. gi¦50510365 Mass: 55788 Score: 31 Expect: 54 Peptides matched: 7 mKIAA0113 protein [Mus musculus] 17. gi¦29881548 Mass: 50164 Score: 31 Expect: 57 Peptides matched: 5 Golgi associated PDZ and coiled-coil motif containing [Mus musculus] 18. gi¦20073181 Mass: 46358 Score: 31 Expect: 57 Peptides matched: 5 Oxa1l protein [Mus musculus] gi¦26346514 Mass: 48760 Score: 29 Expect: 86 Peptides matched: 5 unnamed protein product [Mus musculus] gi¦38372481 Mass: 48702 Score: 29 Expect: 86 Peptides matched: 5 Inner membrane protein OXA1L, mitochondrial precursor (Oxidase assembly 1-like protein) (OXA1- like 19. gi¦47938931 Mass: 71590 Score: 31 Expect: 65 Peptides matched: 6 C030018L16Rik protein [Mus musculus] 20. gi¦14250456 Mass: 73334 Score: 31 Expect: 66 Peptides matched: 8 Tnip1 protein [Mus musculus] gi¦20139295 Mass: 73404 Score: 30 Expect: 70 Peptides matched: 8 Nef-associated factor 1 (Naf1) (A20-binding inhibitor of NF-kappa B activation) (ABIN) (Virion-asso gi¦14198253 Mass: 73460 Score: 30 Expect: 70 Peptides matched: 8 TNFAIP3 interacting protein 1 [Mus musculus] gi¦4995751 Mass: 67556 Score: 29 Expect: 98 Peptides matched: 8 ABINs, A20-binding inhibitor of NF-kappa B activation (small) [Mus musculus] Search Parameters Type of search : Peptide Mass Fingerprint Enzyme : Trypsin Fixed modifications : Carbamidomethyl (C) Mass values : Monoisotopic Protein Mass : Unrestricted Peptide Mass Tolerance : ± 0.3 Da Peptide Charge State : 1+ Max Missed Cleavages : 2 Data File Name : F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data¥PMFtrim¥trim +LS2N-S005 B1_0001.mgf Number of queries : 32 Mascot: http://www.matrixscience.com/ Mascot Search Results User : Email : Search title : MS data file : F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Database : NCBInr 20040815 (1986685 sequences; 666719865 residues) Taxonomy : Mus. (74538 sequences) Timestamp : 28 Sep 2004 at 10:53:10 GMT Top Score : 95 for gi¦33585712, Car6 protein [Mus musculus] Probability Based Mowse Score Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 61 are significant (p<0.05). Protein Summary Report Switch to Concise Protein Summary Report To create a bookmark for this report, right click this link: Protein Summary Report (../data/20040928/F032077.dat) Index Accession Mass Score Description 1. gi¦33585712 36555 95 Car6 protein [Mus musculus] 2. gi¦29612611 37325 94 Car6 protein [Mus musculus] 3. gi¦5921196 36440 81 Carbonic anhydrase VI precursor (Carbonate dehydratase VI) (CA-VI) (Secreted carbonic anhydrase) (S 4. gi¦28461317 29221 77 Car6 protein [Mus musculus] 5. gi¦3421369 30239 61 stress-inducible intra-cellular carbonic anhydrase isozyme VI [Mus musculus] 6. gi¦90689 11778 44 Ig heavy chain precursor V region (BFL23) - mouse (fragment) 7. gi¦1334038 13001 42 unnamed protein product [Mus musculus] 8. gi¦12861258 18421 38 unnamed protein product [Mus musculus] 9. gi¦12860679 19673 37 unnamed protein product [Mus musculus] 10. gi¦26331700 62029 37 unnamed protein product [Mus musculus] 11. gi¦12858423 63029 37 unnamed protein product [Mus musculus] 12. gi¦13508539 67012 37 CLASP2 [Mus musculus] 13. gi¦1166506 83772 37 Stat3B 14. gi¦17512414 83758 37 Signal transducer and activator of transcription 3, isoform 3 [Mus musculus] 15. gi¦11321424 65849 36 Ral-A exchange factor RalGPS2 [Mus musculus] 16. gi¦31560036 65845 36 Ral-A exchange factor RalGPS2 [Mus musculus] 17. gi¦2137361 35986 36 GPI-anchored protein - mouse (fragment) 18. gi¦458706 88809 35 Stat3 19. gi¦34559410 88796 35 signal transducer and activator of transcription 3 [Mus musculus] 20. gi¦13529410 41732 35 Nf2 protein [Mus musculus] Results List 1. gi¦33585712 Mass: 36555 Score: 95 Expect: 2.3e-005 Peptides matched: 10 Car6 protein [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 786.73 785.72 785.43 0.30 46 - 52 0 QSPIDVK 1114.82 1113.82 1113.53 0.29 108 - 116 0 AFHFHWGGR 1144.83 1143.82 1143.70 0.12 156 - 166 0 NGLAVLAVLFK 1330.83 1329.82 1329.71 0.11 185 - 196 0 NIEKPGETTTLK 1459.93 1458.92 1458.71 0.21 132 - 143 0 SIMEAHFVHFNK 1714.94 1713.93 1713.80 0.14 117 - 131 0 DWELSGSEHTIDGIR 2107.97 2106.96 2107.00 -0.04 167 - 184 0 IDEYAENTYYSDIISALK 2338.05 2337.04 2337.19 -0.15 53 - 72 0 TEEVMFNPSLKPLSLVNYEK 2502.02 2501.02 2501.23 -0.21 272 - 292 0 VVEANFLNVPDMYSSYHLYLK 3145.63 3144.62 3144.56 0.06 236 - 263 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 880.61, 1074.82, 1171.90, 1252.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 2. gi¦29612611 Mass: 37325 Score: 94 Expect: 2.9e-005 Peptides matched: 10 Car6 protein [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 786.73 785.72 785.43 0.30 54 - 60 0 QSPIDVK 1114.82 1113.82 1113.53 0.29 116 - 124 0 AFHFHWGGR 1144.83 1143.82 1143.70 0.12 164 - 174 0 NGLAVLAVLFK 1330.83 1329.82 1329.71 0.11 193 - 204 0 NIEKPGETTTLK 1459.93 1458.92 1458.71 0.21 140 - 151 0 SIMEAHFVHFNK 1714.94 1713.93 1713.80 0.14 125 - 139 0 DWELSGSEHTIDGIR 2107.97 2106.96 2107.00 -0.04 175 - 192 0 IDEYAENTYYSDIISALK 2338.05 2337.04 2337.19 -0.15 61 - 80 0 TEEVMFNPSLKPLSLVNYEK 2502.02 2501.02 2501.23 -0.21 280 - 300 0 VVEANFLNVPDMYSSYHLYLK 3145.63 3144.62 3144.56 0.06 244 - 271 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 880.61, 1074.82, 1171.90, 1252.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 3. gi¦5921196 Mass: 36440 Score: 81 Expect: 0.00065 Peptides matched: 9 Carbonic anhydrase VI precursor (Carbonate dehydratase VI) (CA-VI) (Secreted carbonic anhydrase) (S Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 786.73 785.72 785.43 0.30 46 - 52 0 QSPIDVK 1114.82 1113.82 1113.53 0.29 108 - 116 0 AFHFHWGGR 1144.83 1143.82 1143.70 0.12 156 - 166 0 NGLAVLAVLFK 1171.90 1170.90 1170.66 0.24 197 - 206 1 DTTIRDLLPK 1459.93 1458.92 1458.71 0.21 132 - 143 0 SIMEAHFVHFNK 1714.94 1713.93 1713.80 0.14 117 - 131 0 DWELSGSEHTIDGIR 2107.97 2106.96 2107.00 -0.04 167 - 184 0 IDEYAENTYYSDIISALK 2338.05 2337.04 2337.19 -0.15 53 - 72 0 TEEVMFNPSLKPLSLVNYEK 3145.63 3144.62 3144.56 0.06 236 - 263 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 880.61, 1074.82, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 4. gi¦28461317 Mass: 29221 Score: 77 Expect: 0.0016 Peptides matched: 8 Car6 protein [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 1114.82 1113.82 1113.53 0.29 42 - 50 0 AFHFHWGGR 1144.83 1143.82 1143.70 0.12 90 - 100 0 NGLAVLAVLFK 1330.83 1329.82 1329.71 0.11 119 - 130 0 NIEKPGETTTLK 1459.93 1458.92 1458.71 0.21 66 - 77 0 SIMEAHFVHFNK 1714.94 1713.93 1713.80 0.14 51 - 65 0 DWELSGSEHTIDGIR 2107.97 2106.96 2107.00 -0.04 101 - 118 0 IDEYAENTYYSDIISALK 2502.02 2501.02 2501.23 -0.21 206 - 226 0 VVEANFLNVPDMYSSYHLYLK 3145.63 3144.62 3144.56 0.06 170 - 197 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1171.90, 1252.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2338.05, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 5. gi¦3421369 Mass: 30239 Score: 61 Expect: 0.054 Peptides matched: 7 stress-inducible intra-cellular carbonic anhydrase isozyme VI [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 1114.82 1113.82 1113.53 0.29 52 - 60 0 AFHFHWGGR 1144.83 1143.82 1143.70 0.12 100 - 110 0 NGLAVLAVLFK 1171.90 1170.90 1170.66 0.24 141 - 150 1 DTTIRDLLPK 1459.93 1458.92 1458.71 0.21 76 - 87 0 SIMEAHFVHFNK 1714.94 1713.93 1713.80 0.14 61 - 75 0 DWELSGSEHTIDGIR 2107.97 2106.96 2107.00 -0.04 111 - 128 0 IDEYAENTYYSDIISALK 3145.63 3144.62 3144.56 0.06 180 - 207 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 6. gi¦90689 Mass: 11778 Score: 44 Expect: 3.2 Peptides matched: 4 Ig heavy chain precursor V region (BFL23) - mouse (fragment) Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.49 0.08 71 - 77 1 SRLTISK 1653.96 1652.95 1652.92 0.03 64 - 77 2 YYNPALKSRLTISK 2026.92 2025.92 2026.08 -0.17 71 - 87 2 SRLTISKDTYNNQVFLK 3070.66 3069.65 3069.61 0.05 45 - 70 2 QPSGKGLEWLLHILWNDSKYYNPALK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 7. gi¦1334038 Mass: 13001 Score: 42 Expect: 5 Peptides matched: 4 unnamed protein product [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.49 0.08 63 - 69 1 SRLTISK 1653.96 1652.95 1652.92 0.03 56 - 69 2 YYNPALKSRLTISK 2026.92 2025.92 2026.08 -0.17 63 - 79 2 SRLTISKDTYNNQVFLK 3070.66 3069.65 3069.61 0.05 37 - 62 2 QPSGKGLEWLLHILWNDSKYYNPALK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 8. gi¦12861258 Mass: 18421 Score: 38 Expect: 11 Peptides matched: 4 unnamed protein product [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 864.60 863.59 863.43 0.16 82 - 88 1 QSSQKMR 1714.94 1713.93 1713.91 0.02 29 - 43 1 AQDPSLLSNRLMIEK 2920.30 2919.29 2919.48 -0.19 93 - 116 2 TLMEETTRQQSMIRELIETNQQLK 3279.90 3278.90 3278.61 0.29 117 - 144 2 SELQLEQNRAAHQEQRANDLQQIMDSVK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3293.91 9. gi¦12860679 Mass: 19673 Score: 37 Expect: 14 Peptides matched: 4 unnamed protein product [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 864.60 863.59 863.43 0.16 82 - 88 1 QSSQKMR 1714.94 1713.93 1713.91 0.02 29 - 43 1 AQDPSLLSNRLMIEK 2920.30 2919.29 2919.48 -0.19 93 - 116 2 TLMEETTRQQSMIRELIETNQQLK 3279.90 3278.90 3278.61 0.29 117 - 144 2 SELQLEQNRAAHQEQRANDLQQIMDSVK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3293.91 10. gi¦26331700 Mass: 62029 Score: 37 Expect: 14 Peptides matched: 6 unnamed protein product [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.45 0.12 472 - 478 1 SLKATER 1074.82 1073.81 1073.57 0.24 194 - 202 0 SNLMNNILR 1368.99 1367.98 1367.78 0.20 245 - 257 1 LSLKIEPGASTPR 2920.30 2919.29 2919.44 -0.15 249 - 278 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 3175.67 3174.67 3174.47 0.20 488 - 515 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK 3279.90 3278.90 3278.70 0.19 408 - 438 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 11. gi¦12858423 Mass: 63029 Score: 37 Expect: 15 Peptides matched: 6 unnamed protein product [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.45 0.12 507 - 513 1 SLKATER 1074.82 1073.81 1073.57 0.24 229 - 237 0 SNLMNNILR 1368.99 1367.98 1367.78 0.20 280 - 292 1 LSLKIEPGASTPR 2920.30 2919.29 2919.44 -0.15 284 - 313 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 3175.67 3174.67 3174.47 0.20 523 - 550 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK 3279.90 3278.90 3278.70 0.19 443 - 473 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 12. gi¦13508539 Mass: 67012 Score: 37 Expect: 16 Peptides matched: 7 CLASP2 [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.43 0.14 204 - 209 1 IDLCKR 1353.00 1352.00 1351.72 0.27 369 - 379 1 LLKELSNHNER 1459.93 1458.92 1458.88 0.04 431 - 442 2 VLKEILRHQPAR 1653.96 1652.95 1652.82 0.13 372 - 384 2 ELSNHNERIEERK 2026.92 2025.92 2025.95 -0.03 223 - 240 1 FDEVQNSGGMILSVCKDK 2951.69 2950.69 2950.39 0.29 239 - 266 2 DKSFDDEESVDGNRPSSAASAFKVPAPK 3293.91 3292.90 3292.62 0.27 17 - 47 1 DVSGRLQAGEELLLCLGTPGAIPDLEDDPSR No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1368.99, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90 13. gi¦1166506 Mass: 83772 Score: 37 Expect: 16 Peptides matched: 7 Stat3B Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 727.74 726.73 726.51 0.22 349 - 354 1 VRLLVK 776.64 775.63 775.46 0.17 309 - 314 0 IVELFR 1252.83 1251.82 1251.61 0.21 616 - 626 0 EGGVTFTWVEK 1653.96 1652.95 1652.83 0.12 410 - 423 2 HLTLREQRCGNGGR 2502.02 2501.02 2501.13 -0.11 686 - 707 0 YCRPESQEHPEADPGSAAPYLK 2920.30 2919.29 2919.40 -0.10 393 - 417 2 VMNMEESNNGSLSAEFKHLTLREQR 3161.64 3160.63 3160.56 0.07 632 - 658 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 14. gi¦17512414 Mass: 83758 Score: 37 Expect: 16 Peptides matched: 7 Signal transducer and activator of transcription 3, isoform 3 [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 727.74 726.73 726.51 0.22 349 - 354 1 VRLLVK 776.64 775.63 775.46 0.17 309 - 314 0 IVELFR 1252.83 1251.82 1251.61 0.21 616 - 626 0 EGGVTFTWVEK 1653.96 1652.95 1652.83 0.12 410 - 423 2 HLTLREQRCGNGGR 2502.02 2501.02 2501.13 -0.11 686 - 707 0 YCRPESQEHPEADPGSAAPYLK 2920.30 2919.29 2919.40 -0.10 393 - 417 2 VMNMEESNNGSLSAEFKHLTLREQR 3161.64 3160.63 3160.56 0.07 632 - 658 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 15. gi¦11321424 Mass: 65849 Score: 36 Expect: 18 Peptides matched: 6 Ral-A exchange factor RalGPS2 [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.45 0.12 507 - 513 1 SLKATER 1074.82 1073.81 1073.57 0.24 229 - 237 0 SNLMNNILR 1368.99 1367.98 1367.78 0.20 280 - 292 1 LSLKIEPGASTPR 2920.30 2919.29 2919.44 -0.15 284 - 313 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 3175.67 3174.67 3174.47 0.20 523 - 550 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK 3279.90 3278.90 3278.70 0.19 443 - 473 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 16. gi¦31560036 Mass: 65845 Score: 36 Expect: 18 Peptides matched: 6 Ral-A exchange factor RalGPS2 [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 804.58 803.57 803.45 0.12 507 - 513 1 SLKATER 1074.82 1073.81 1073.57 0.24 229 - 237 0 SNLMNNILR 1368.99 1367.98 1367.78 0.20 280 - 292 1 LSLKIEPGASTPR 2920.30 2919.29 2919.44 -0.15 284 - 313 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 3175.67 3174.67 3174.47 0.20 523 - 550 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK 3279.90 3278.90 3278.70 0.19 443 - 473 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 17. gi¦2137361 Mass: 35986 Score: 36 Expect: 18 Peptides matched: 5 GPI-anchored protein - mouse (fragment) Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 1144.83 1143.82 1143.67 0.15 1 - 10 1 MKQILGVIDK 1330.83 1329.82 1329.67 0.15 139 - 149 1 LVDPERDMSLR 1653.96 1652.95 1652.76 0.19 22 - 34 2 LDDYQERMNKGER 2294.95 2293.95 2294.13 -0.19 150 - 168 0 LNEQYEHASIHLWDLLEGK 3175.67 3174.67 3174.63 0.03 117 - 144 1 QGLSGVPILSEEELSLLDEFYKLVDPER No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1171.90, 1252.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3279.90, 3293.91 18. gi¦458706 Mass: 88809 Score: 35 Expect: 24 Peptides matched: 7 Stat3 Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 727.74 726.73 726.51 0.22 349 - 354 1 VRLLVK 776.64 775.63 775.46 0.17 309 - 314 0 IVELFR 1252.83 1251.82 1251.61 0.21 616 - 626 0 EGGVTFTWVEK 1653.96 1652.95 1652.83 0.12 410 - 423 2 HLTLREQRCGNGGR 2502.02 2501.02 2501.13 -0.11 686 - 707 0 YCRPESQEHPEADPGSAAPYLK 2920.30 2919.29 2919.40 -0.10 393 - 417 2 VMNMEESNNGSLSAEFKHLTLREQR 3161.64 3160.63 3160.56 0.07 632 - 658 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 19. gi¦34559410 Mass: 88796 Score: 35 Expect: 24 Peptides matched: 7 signal transducer and activator of transcription 3 [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 727.74 726.73 726.51 0.22 349 - 354 1 VRLLVK 776.64 775.63 775.46 0.17 309 - 314 0 IVELFR 1252.83 1251.82 1251.61 0.21 616 - 626 0 EGGVTFTWVEK 1653.96 1652.95 1652.83 0.12 410 - 423 2 HLTLREQRCGNGGR 2502.02 2501.02 2501.13 -0.11 686 - 707 0 YCRPESQEHPEADPGSAAPYLK 2920.30 2919.29 2919.40 -0.10 393 - 417 2 VMNMEESNNGSLSAEFKHLTLREQR 3161.64 3160.63 3160.56 0.07 632 - 658 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 20. gi¦13529410 Mass: 41732 Score: 35 Expect: 24 Peptides matched: 6 Nf2 protein [Mus musculus] Observed Mr(expt) Mr(calc) Delta Start End Miss Peptide 749.74 748.73 748.45 0.28 279 - 284 1 KIDVFK 776.64 775.63 775.40 0.23 1 - 8 0 MAGAIASR 1144.83 1143.82 1143.57 0.25 323 - 331 2 AQAREERER 2294.95 2293.95 2294.19 -0.25 229 - 249 0 GTELLLGVDALGLHIYDPENR 2920.30 2919.29 2919.54 -0.25 124 - 149 2 KQILDEKVYCPPEASVLLASYAVQAK 2951.69 2950.69 2950.48 0.21 100 - 123 0 FYPENAEEELVQEITQHLFFLQVK No match to: 727.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2338.05, 2502.02, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 Search Parameters Type of search : Peptide Mass Fingerprint Enzyme : Trypsin Fixed modifications : Carbamidomethyl (C) Mass values : Monoisotopic Protein Mass : Unrestricted Peptide Mass Tolerance : ± 0.3 Da Peptide Charge State : 1+ Max Missed Cleavages : 2 Data File Name : F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Number of queries : 32 Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦33585712 Score: 95 Expect: 2.3e-005 Car6 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 36555; Calculated pI value: 6.11 NCBI BLAST search of gi¦33585712 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 10 Sequence Coverage: 48% Matched peptides shown in Bold Red 1 MRALVSVVSL FFLGIQAHSD WSYSGDDGVG ESQWSEQYPS CGGERQSPID 51 VKTEEVMFNP SLKPLSLVNY EKENLEFTMT NNGHTVSIDL PPSMYLETSD 101 GTEFISKAFH FHWGGRDWEL SGSEHTIDGI RSIMEAHFVH FNKEYGTYEN 151 AKDQKNGLAV LAVLFKIDEY AENTYYSDII SALKNIEKPG ETTTLKDTTI 201 RNLLPKDVHH YYTYPGSLTT PPCTENVQWF VLRDKVTLSK AQVVTIENSV 251 MDHNNNTIQN GYRSTQPNNH RVVEANFLNV PDMYSSYHLY LKNMQKEILQ 301 PKKQKKTKKN RHFWSRK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 46 - 52 786.73 785.72 785.43 0.30 0 QSPIDVK 53 - 72 2338.05 2337.04 2337.19 -0.15 0 TEEVMFNPSLKPLSLVNYEK 108 - 116 1114.82 1113.82 1113.53 0.29 0 AFHFHWGGR 117 - 131 1714.94 1713.93 1713.80 0.14 0 DWELSGSEHTIDGIR 132 - 143 1459.93 1458.92 1458.71 0.21 0 SIMEAHFVHFNK 156 - 166 1144.83 1143.82 1143.70 0.12 0 NGLAVLAVLFK 167 - 184 2107.97 2106.96 2107.00 -0.04 0 IDEYAENTYYSDIISALK 185 - 196 1330.83 1329.82 1329.71 0.11 0 NIEKPGETTTLK 236 - 263 3145.63 3144.62 3144.56 0.06 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR 272 - 292 2502.02 2501.02 2501.23 -0.21 0 VVEANFLNVPDMYSSYHLYLK No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 880.61, 1074.82, 1171.90, 1252.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH55437 317 aa linear ROD 08-OCT-2003 DEFINITION Car6 protein [Mus musculus]. ACCESSION AAH55437 VERSION AAH55437.1 GI:33585712 DBSOURCE accession BC055437.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 317) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse, L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C. M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer, C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang, J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci, P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R. D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J. A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu, X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green, E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers, R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REFERENCE 2 (residues 1 to 317) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (01-AUG-2003) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih. gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey E. Green, M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I. M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http://www-shgc.stanford.edu Contact: (Dickson, Mark) mcd@paxil.stanford.edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 120 Row: j Column: 21 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 6753269. Method: conceptual translation. FEATURES Location/Qualifiers source 1..317 / organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" / clone="MGC:65279 IMAGE:4190566" /tissue_type="Salivary gland, 10 week old female mouse" /clone_lib="NCI_CGAP_SG2" /lab_host="DH10B" / note="Vector: pCMV-SPORT6" Protein 1..317 /product="Car6 protein" Region 21..276 /region_name="Eukaryotic-type carbonic anhydrase" / note="carb_anhydrase" /db_xref="CDD:pfam00194" Region 21..276 / region_name="Eukaryotic-type carbonic anhydrase" / note="Carb_anhydrase" /db_xref="CDD:25435" CDS 1..317 /gene="Car6" / coded_by="BC055437.1:13..966" /db_xref="LocusID:12353" / db_xref="MGI:1333786" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦29612611 Score: 94 Expect: 2.9e-005 Car6 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 37325; Calculated pI value: 6.17 NCBI BLAST search of gi¦29612611 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 10 Sequence Coverage: 47% Matched peptides shown in Bold Red 1 VAHAYASAMR ALVSVVSLFF LGIQAHSDWS YSGDDGVGES QWSEQYPSCG 51 GERQSPIDVK TEEVMFNPSL KPLSLVNYEK ENLEFTMTNN GHTVSIDLPP 101 SMYLETSDGT EFISKAFHFH WGGRDWELSG SEHTIDGIRS IMEAHFVHFN 151 KEYGTYENAK DQKNGLAVLA VLFKIDEYAE NTYYSDIISA LKNIEKPGET 201 TTLKDTTIRN LLPKDVHHYY TYPGSLTTPP CTENVQWFVL RDKVTLSKAQ 251 VVTIENSVMD HNNNTIQNGY RSTQPNNHRV VEANFLNVPD MYSSYHLYLK 301 NMQKEILQPK KQKKTKKNRH FWSRK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 54 - 60 786.73 785.72 785.43 0.30 0 QSPIDVK 61 - 80 2338.05 2337.04 2337.19 -0.15 0 TEEVMFNPSLKPLSLVNYEK 116 - 124 1114.82 1113.82 1113.53 0.29 0 AFHFHWGGR 125 - 139 1714.94 1713.93 1713.80 0.14 0 DWELSGSEHTIDGIR 140 - 151 1459.93 1458.92 1458.71 0.21 0 SIMEAHFVHFNK 164 - 174 1144.83 1143.82 1143.70 0.12 0 NGLAVLAVLFK 175 - 192 2107.97 2106.96 2107.00 -0.04 0 IDEYAENTYYSDIISALK 193 - 204 1330.83 1329.82 1329.71 0.11 0 NIEKPGETTTLK 244 - 271 3145.63 3144.62 3144.56 0.06 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR 280 - 300 2502.02 2501.02 2501.23 -0.21 0 VVEANFLNVPDMYSSYHLYLK No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 880.61, 1074.82, 1171.90, 1252.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH49973 325 aa linear ROD 10-JUN-2003 DEFINITION Car6 protein [Mus musculus]. ACCESSION AAH49973 VERSION AAH49973.1 GI:29612611 DBSOURCE accession BC049973.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 325) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse, L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C. M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer, C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang, J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci, P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R. D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J. A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu, X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green, E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers, R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REFERENCE 2 (residues 1 to 325) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (31-MAR-2003) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih. gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey E. Green, M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I. M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S. M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley, C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D., McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P. J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K. D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 108 Row: c Column: 18 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 6753269. Method: conceptual translation. FEATURES Location/Qualifiers source 1..325 / organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" / clone="IMAGE:4193056" /tissue_type="Salivary gland, 10 week old female mouse" /clone_lib="NCI_CGAP_SG2" /lab_host="DH10B" / note="Vector: pCMV-SPORT6" Protein 1..325 /product="Car6 protein" Region 29..284 /region_name="Eukaryotic-type carbonic anhydrase" / note="Carb_anhydrase" /db_xref="CDD:25435" CDS 1..325 /gene="Car6" / coded_by="BC049973.1:<1..978" /db_xref="LocusID:12353" / db_xref="MGI:1333786" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦28461317 Score: 77 Expect: 0.0016 Car6 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 29221; Calculated pI value: 7.96 NCBI BLAST search of gi¦28461317 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 8 Sequence Coverage: 50% Matched peptides shown in Bold Red 1 RTRGEKENLE FTMTNNGHTV SIDLPPSMYL ETSDGTEFIS KAFHFHWGGR 51 DWELSGSEHT IDGIRSIMEA HFVHFNKEYG TYENAKDQKN GLAVLAVLFK 101 IDEYAENTYY SDIISALKNI EKPGETTTLK DTTIRNLLPK DVHHYYTYPG 151 SLTTPPCTEN VQWFVLRDKV TLSKAQVVTI ENSVMDHNNN TIQNGYRSTQ 201 PNNHRVVEAN FLNVPDMYSS YHLYLKNMQK EILQPKKQKK TKKNRHFWSR 251 K Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 42 - 50 1114.82 1113.82 1113.53 0.29 0 AFHFHWGGR 51 - 65 1714.94 1713.93 1713.80 0.14 0 DWELSGSEHTIDGIR 66 - 77 1459.93 1458.92 1458.71 0.21 0 SIMEAHFVHFNK 90 - 100 1144.83 1143.82 1143.70 0.12 0 NGLAVLAVLFK 101 - 118 2107.97 2106.96 2107.00 -0.04 0 IDEYAENTYYSDIISALK 119 - 130 1330.83 1329.82 1329.71 0.11 0 NIEKPGETTTLK 170 - 197 3145.63 3144.62 3144.56 0.06 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR 206 - 226 2502.02 2501.02 2501.23 -0.21 0 VVEANFLNVPDMYSSYHLYLK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1171.90, 1252.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2338.05, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH46495 251 aa linear ROD 10-JUN-2003 DEFINITION Car6 protein [Mus musculus]. ACCESSION AAH46495 VERSION AAH46495.1 GI:28461317 DBSOURCE accession BC046495.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 251) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse, L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C. M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer, C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang, J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci, P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R. D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J. A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu, X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green, E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers, R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REFERENCE 2 (residues 1 to 251) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (03-FEB-2003) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih. gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey E. Green, M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I. M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, A. N., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 37 Row: e Column: 20 This clone was selected for full length sequencing because it passed the following selection criteria: Similarity but not identity to protein. Method: conceptual translation. FEATURES Location/ Qualifiers source 1..251 /organism="Mus musculus" /strain="FVB/N" / db_xref="taxon:10090" /clone="IMAGE:4190426" /tissue_type="Salivary gland, 10 week old female mouse" /clone_lib="NCI_CGAP_SG2" / lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..251 / product="Car6 protein" Region <12..210 /region_name="Eukaryotictype carbonic anhydrase" /note="Carb_anhydrase" / db_xref="CDD:25435" CDS 1..251 /gene="Car6" /coded_by="BC046495.1: <1..756" /db_xref="LocusID:12353" /db_xref="MGI:1333786" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦5921196 Score: 81 Expect: 0.00065 Carbonic anhydrase VI precursor (Carbonate dehydratase VI) (CA-VI) (Secreted carbonic anhydrase) (S Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 36440; Calculated pI value: 5.91 NCBI BLAST search of gi¦5921196 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦3421371 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 9 Sequence Coverage: 41% Matched peptides shown in Bold Red 1 MRALVSVVSL FFLGIQAHSD WSYSGDDGVG ESQWSEQYPS CGGERQSPID 51 VKTEEVMFNP SLKPLSLVNY EKENLEFTMT NNGHTVSIDL PPSMYLETSD 101 GTEFISKAFH FHWGGRDWEL SGSEHTIDGI RSIMEAHFVH FNKEYGTYEN 151 AKDQKNGLAV LAVLFKIDEY AENTYYSDII SALKDIEKPG ETTTLKDTTI 201 RDLLPKDVHH YYTYPGSLTT PPCTENVQWF VLRDRVTLSK AQVVTIENSV 251 MDHNNNTIQN GYRSTQPNNH RVVEANFLNV PDMYSSYHLY PKNMQKEILQ 301 PKKQKKTKKN RHFGSRK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 46 - 52 786.73 785.72 785.43 0.30 0 QSPIDVK 53 - 72 2338.05 2337.04 2337.19 -0.15 0 TEEVMFNPSLKPLSLVNYEK 108 - 116 1114.82 1113.82 1113.53 0.29 0 AFHFHWGGR 117 - 131 1714.94 1713.93 1713.80 0.14 0 DWELSGSEHTIDGIR 132 - 143 1459.93 1458.92 1458.71 0.21 0 SIMEAHFVHFNK 156 - 166 1144.83 1143.82 1143.70 0.12 0 NGLAVLAVLFK 167 - 184 2107.97 2106.96 2107.00 -0.04 0 IDEYAENTYYSDIISALK 197 - 206 1171.90 1170.90 1170.66 0.24 1 DTTIRDLLPK 236 - 263 3145.63 3144.62 3144.56 0.06 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 880.61, 1074.82, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS P18761 317 aa linear ROD 15-JUN-2004 DEFINITION Carbonic anhydrase VI precursor (Carbonate dehydratase VI) (CA-VI) (Secreted carbonic anhydrase) (Salivary carbonic anhydrase). ACCESSION P18761 VERSION P18761 GI:5921196 DBSOURCE swissprot: locus CAH6_MOUSE, accession P18761; class: standard. extra accessions:O88625,created: Nov 1, 1990. sequence updated: Jul 15, 1999. annotation updated: Jun 15, 2004. xrefs: gi: 3421370, gi: 3421371, gi: 90358 xrefs (nonsequence databases): HSSPP00918, MGI1333786, InterProIPR001148, PfamPF00194, ProDomPD000865, PROSITEPS00162 KEYWORDS Lyase; Zinc; Glycoprotein; Signal; Direct protein sequencing. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 317) AUTHORS Sok,J., Wang,X.Z., Batchvarova,N., Kuroda,M., Harding,H. and Ron,D. TITLE CHOP-Dependent stressinducible expression of a novel form of carbonic anhydrase VI JOURNAL Mol. Cell. Biol. 19 (1), 495-504 (1999) MEDLINE 99077987 PUBMED 9858573 REMARK SEQUENCE FROM N.A. STRAIN=CD-1; TISSUE=Salivary gland REFERENCE 2 (residues 1 to 317) AUTHORS Fernley,R.T., Darling,P., Aldred,P., Wright,R.D. and Coghlan,J.P. TITLE Tissue and species distribution of the secreted carbonic anhydrase isoenzyme JOURNAL Biochem. J. 259 (1), 91-96 (1989) MEDLINE 89246331 PUBMED 2497732 REMARK SEQUENCE OF 18-38. COMMENT On Sep 23, 1999 this sequence version replaced gi:115468. ------------------------------------------------------------------- This SWISS-PROT entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. The original entry is available from http://www.expasy.ch/sprot and http://www.ebi.ac.uk/sprot ------------------------------------------------------------------. [FUNCTION] Reversible hydration of carbon dioxide. Its role in saliva is unknown. [CATALYTIC ACTIVITY] H(2)CO(3) = CO(2) + H(2)O. [COFACTOR] Zinc (By similarity). [SUBCELLULAR LOCATION] Secreted. [TISSUE SPECIFICITY] Major consistuent of saliva. [SIMILARITY] Belongs to the eukaryotic-type carbonic anhydrase family. FEATURES Location/Qualifiers source 1..317 /organism="Mus musculus" / db_xref="taxon:10090" gene 1..317 /gene="CA6" /note="synonym: CAR6" Protein 1..317 /gene="CA6" /product="Carbonic anhydrase VI precursor" /EC_number="4.2.1.1" Region 1..17 /gene="CA6" / region_name="Signal" Region 18..317 /gene="CA6" / region_name="Mature chain" /note="Carbonic anhydrase VI." Region 18 /gene="CA6" /region_name="Conflict" /note="H -> G (in Ref. 2)." Region 21..276 /gene="CA6" /region_name="Eukaryotic-type carbonic anhydrase" /note="Carb_anhydrase" /db_xref="CDD:25435" Region 35 / gene="CA6" /region_name="Conflict" /note="S -> T (in Ref. 2)." Bond bond(41,223) /gene="CA6" /bond_type="disulfide" /note="Potential." Site 110 /gene="CA6" /site_type="metal-binding" /note="Zinc (catalytic) (By similarity)." Site 112 /gene="CA6" / site_type="metal-binding" /note="Zinc (catalytic) (By similarity)." Site 137 /gene="CA6" /site_type="metal-binding" /note="Zinc (catalytic) (By similarity)." Site 255 /gene="CA6" / site_type="glycosylation" /note="N-linked (GlcNAc...) (Potential)." Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦3421369 Score: 61 Expect: 0.054 stress-inducible intra-cellular carbonic anhydrase isozyme VI [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 30239; Calculated pI value: 6.64 NCBI BLAST search of gi¦3421369 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦6753270 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 39% Matched peptides shown in Bold Red 1 MFNPSLKPLS LVNYEKENLE FTMTNNGHTV SIDLPPSMYL ETSDGTEFIS 51 KAFHFHWGGR DWELSGSEHT IDGIRSIMEA HFVHFNKEYG TYENAKDQKN 101 GLAVLAVLFK IDEYAENTYY SDIISALKDI EKPGETTTLK DTTIRDLLPK 151 DVHHYYTYPG SLTTPPCTEN VQWFVLRDRV TLSKAQVVTI ENSVMDHNNN 201 TIQNGYRSTQ PNNHRVVEAN FLNVPDMYSS YHLYPKNMQK EILQPKKQKK 251 TKKNRHFGSR K Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 52 - 60 1114.82 1113.82 1113.53 0.29 0 AFHFHWGGR 61 - 75 1714.94 1713.93 1713.80 0.14 0 DWELSGSEHTIDGIR 76 - 87 1459.93 1458.92 1458.71 0.21 0 SIMEAHFVHFNK 100 - 110 1144.83 1143.82 1143.70 0.12 0 NGLAVLAVLFK 111 - 128 2107.97 2106.96 2107.00 -0.04 0 IDEYAENTYYSDIISALK 141 - 150 1171.90 1170.90 1170.66 0.24 1 DTTIRDLLPK 180 - 207 3145.63 3144.62 3144.56 0.06 1 VTLSKAQVVTIENSVMDHNNNTIQNGYR No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 2026.92, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAD12539 261 aa linear ROD 04-FEB-1999 DEFINITION stressinducible intra-cellular carbonic anhydrase isozyme VI [Mus musculus]. ACCESSION AAD12539 VERSION AAD12539.1 GI:3421369 DBSOURCE locus AF079834 accession AF079834.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 261) AUTHORS Wang,X.Z., Kuroda,M., Sok,J., Batchvarova,N., Kimmel,R., Chung,P., Zinszner,H. and Ron,D. TITLE Identification of novel stress-induced genes downstream of chop JOURNAL EMBO J. 17 (13), 3619-3630 (1998) MEDLINE 98315054 PUBMED 9649432 REFERENCE 2 (residues 1 to 261) AUTHORS Sok,J., Wang,X.Z., Batchvarova,N., Kuroda,M., Harding,H. and Ron,D. TITLE CHOPDependent stress-inducible expression of a novel form of carbonic anhydrase VI JOURNAL Mol. Cell. Biol. 19 (1), 495-504 (1999) MEDLINE 99077987 PUBMED 9858573 REFERENCE 3 (residues 1 to 261) AUTHORS Sok,J., Wang,X.Z. and Ron,D. TITLE Direct Submission JOURNAL Submitted (22-JUL-1998) Skirball Institute, New York University Medical Center, 540 First Ave., New York, NY 10016, USA COMMENT Method: conceptual translation supplied by author. FEATURES Location/Qualifiers source 1..261 /organism="Mus musculus" / strain="NIH Swiss" /db_xref="taxon:10090" /cell_line="NIH-3T3" Protein 1..261 /product="stress-inducible intra-cellular carbonic anhydrase isozyme VI" Region 2..220 /region_name="Eukaryotic-type carbonic anhydrase" /note="Carb_anhydrase" /db_xref="CDD:25435" CDS 1..261 /coded_by="AF079834.1:285..1070" /note="alternatively spliced" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦90689 Score: 44 Expect: 3.2 Ig heavy chain precursor V region (BFL23) - mouse (fragment) Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 11778; Calculated pI value: 8.90 NCBI BLAST search of gi¦90689 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 4 Sequence Coverage: 41% Matched peptides shown in Bold Red 1 RCLCQVTLKE CGPGILQPSQ TLSLTCSFSG FSLSTSNMGV GWIRQPSGKG 51 LEWLLHILWN DSKYYNPALK SRLTISKDTY NNQVFLKIAN VDTADTATYY 101 CAR Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 45 - 70 3070.66 3069.65 3069.61 0.05 2 QPSGKGLEWLLHILWNDSKYYNPALK 64 - 77 1653.96 1652.95 1652.92 0.03 2 YYNPALKSRLTISK 71 - 77 804.58 803.57 803.49 0.08 1 SRLTISK 71 - 87 2026.92 2025.92 2026.08 -0.17 2 SRLTISKDTYNNQVFLK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS B25913 103 aa linear ROD 16-AUG-1996 DEFINITION Ig heavy chain precursor V region (BFL23) - mouse (fragment). ACCESSION B25913 VERSION B25913 GI:90689 DBSOURCE pir: locus B25913; summary: #length 103 #checksum 957 ; superfamily: immunoglobulin V region; immunoglobulin homology ; PIR dates: 16-Aug-1988 #sequence_revision 16-Aug-1988 #text_change 16-Aug-1996 ; punctuation in sequence. KEYWORDS heterotetramer; immunoglobulin. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 103) AUTHORS Lawler,A.M., Lin,P.S. and Gearhart,P.J. TITLE Adult Bcell repertoire is biased toward two heavy-chain variable-region genes that rearrange frequently in fetal pre-B cells JOURNAL Proc. Natl. Acad. Sci. U.S.A. 84 (8), 2454-2458 (1987) MEDLINE 87175692 PUBMED 3104915 FEATURES Location/Qualifiers source 1..103 / organism="Mus musculus" /db_xref="taxon:10090" Protein 1..103 / product="Ig heavy chain precursor V region (BFL23)" Region 17..103 / region_name="Immunoglobulin domain variable region (v) subfamily" / note="IGv" /db_xref="CDD:24150" Region 19..103 / region_name="domain" /note="immunoglobulin homology #label IMM" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦1334038 Score: 42 Expect: 5 unnamed protein product [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 13001; Calculated pI value: 6.73 NCBI BLAST search of gi¦1334038 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦284860 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 4 Sequence Coverage: 37% Matched peptides shown in Bold Red 1 VESGPGILQP SQTLSLTCSF SGFSLSTSNM GVGWIRQPSG KGLEWLLHIL 51 WNDSKYYNPA LKSRLTISKD TYNNQVFLKI ANVDTADTAT YYCARIANWD 101 WYFDVWGAGT TVTVSS Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 37 - 62 3070.66 3069.65 3069.61 0.05 2 QPSGKGLEWLLHILWNDSKYYNPALK 56 - 69 1653.96 1652.95 1652.92 0.03 2 YYNPALKSRLTISK 63 - 69 804.58 803.57 803.49 0.08 1 SRLTISK 63 - 79 2026.92 2025.92 2026.08 -0.17 2 SRLTISKDTYNNQVFLK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS CAA41908 116 aa linear ROD 20-MAY-1992 DEFINITION unnamed protein product [Mus musculus]. ACCESSION CAA41908 VERSION CAA41908.1 GI:1334038 DBSOURCE embl locus MMIGHT651, accession X59198.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 116) AUTHORS Stark,S.E. and Caton,A.J. TITLE Antibodies that are specific for a single amino acid interchange in a protein epitope use structurally distinct variable regions JOURNAL J. Exp. Med. 174 (3), 613-624 (1991) MEDLINE 91341421 PUBMED 1908510 REFERENCE 2 (residues 1 to 116) AUTHORS Caton,A.J. TITLE Direct Submission JOURNAL Submitted (19-APR-1991) A.J. Caton, The Wistar Institute, 361 Spruce St, Philadelphia, PA 19104, USA COMMENT see x59172-x59211. FEATURES Location/Qualifiers source 1..116 /organism="Mus musculus" / strain="BALB/C" /isolate="T6-510" /db_xref="taxon:10090" / cell_line="T6-510" /cell_type="hybridoma" /tissue_type="B-cell" Protein 1..116 /name="unnamed protein product" Region 9..115 / region_name="Immunoglobulin domain variable region (v) subfamily" / note="IGv" /db_xref="CDD:24150" CDS 1..116 / coded_by="X59198.1:1..350" /note="rearranged heavy chain variable region" /codon_start=3 Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦12861258 Score: 38 Expect: 11 unnamed protein product [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 18421; Calculated pI value: 5.04 NCBI BLAST search of gi¦12861258 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 4 Sequence Coverage: 46% Matched peptides shown in Bold Red 1 MTEGSQIFLL PISTSDSTKE PLSPVASKAQ DPSLLSNRLM IEKQQEEAEW 51 ESINGLLMTH GFKPLCLVKG ADLRDFIVFD KQSSQKMRQI LKTLMEETTR 101 QQSMIRELIE TNQQLKSELQ LEQNRAAHQE QRANDLQQIM DSVKSKIGEL 151 EDESLNRVCQ Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 29 - 43 1714.94 1713.93 1713.91 0.02 1 AQDPSLLSNRLMIEK 82 - 88 864.60 863.59 863.43 0.16 1 QSSQKMR 93 - 116 2920.30 2919.29 2919.48 -0.19 2 TLMEETTRQQSMIRELIETNQQLK 117 - 144 3279.90 3278.90 3278.61 0.29 2 SELQLEQNRAAHQEQRANDLQQIMDSVK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3293.91 LOCUS BAB32153 160 aa linear ROD 03-APR-2004 DEFINITION unnamed protein product [Mus musculus]. ACCESSION BAB32153 VERSION BAB32153.1 GI:12861258 DBSOURCE accession AK020622.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency fulllength cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) MEDLINE 99279253 PUBMED 10349636 REFERENCE 2 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10 (10), 1617-1630 (2000) MEDLINE 20499374 PUBMED 11042159 REFERENCE 3 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai, T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa, M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka, T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M., Inoue,Y., Kira, A. and Hayashizaki,Y. TITLE RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10 (11), 1757-1771 (2000) MEDLINE 20530913 PUBMED 11076861 REFERENCE 4 AUTHORS The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 5 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 6 (residues 1 to 160) AUTHORS Adachi,J., Aizawa,K., Akahira,S., Akimura,T., Arai,A., Aono,H., Arakawa,T., Bono,H., Carninci,P., Fukuda,S., Fukunishi,Y., Furuno,M., Hanagaki,T., Hara,A., Hayatsu,N., Hiramoto,K., Hiraoka, T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Izawa,M., Kasukawa,T., Kato,H., Kawai,J., Kojima,Y., Konno,H., Kouda,M., Koya,S., Kurihara, C., Matsuyama,T., Miyazaki,A., Nishi,K., Nomura,K., Numazaki,R., Ohno,M., Okazaki,Y., Okido,T., Owa,C., Saito,H., Saito,R., Sakai, C., Sakai,K., Sano,H., Sasaki,D., Shibata,K., Shibata,Y., Shinagawa, A., Shiraki,T., Sogabe,Y., Suzuki,H., Tagami,M., Tagawa,A., Takahashi,F., Tanaka,T., Tejima,Y., Toya,T., Yamamura,T., Yasunishi, A., Yoshida,K., Yoshino,M., Muramatsu,M. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (18-AUG-2000) Yoshihide Hayashizaki, The Institute of Physical and Chemical Research (RIKEN), Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (E-mail: genome-res@gsc.riken.jp, URL:http://genome.gsc.riken.jp/, Tel:81-45- 503-9222, Fax:81-45-503-9216) COMMENT Please visit our web site (http://genome.gsc.riken.jp/) for further details. cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. First strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through two rounds of normalization to Rot = 20.0 and subtraction to Rot = 370.4. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. Host: DH10B. FEATURES Location/Qualifiers source 1..160 /organism="Mus musculus" /strain="C57BL/6J" / db_xref="FANTOM_DB:9530064H08" /db_xref="taxon:10090" / clone="9530064H08" /sex="male" /tissue_type="urinary bladder" / clone_lib="RIKEN full-length enriched mouse cDNA library" / dev_stage="adult" Protein 1..160 /name="unnamed protein product" CDS 1..160 /coded_by="AK020622.1:192..>671" /note="putative similar to SIMILAR TO P10-BINDING PROTEIN [Homo sapiens] (SPTR|Q96B31, evidence: FASTY, 77.3%ID, 72.6%length, match=462)" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦12860679 Score: 37 Expect: 14 unnamed protein product [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 19673; Calculated pI value: 5.34 NCBI BLAST search of gi¦12860679 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 4 Sequence Coverage: 43% Matched peptides shown in Bold Red 1 MTEGSQIFLL PISTSDSTKE PLSPVASKAQ DPSLLSNRLM IEKQQEEAEW 51 ESINGLLMTH GFKPLCLVKG ADLRDFIVFD KQSSQKMRQI LKTLMEETTR 101 QQSMIRELIE TNQQLKSELQ LEQNRAAHQE QRANDLQQIM DSVKSKIGEL 151 EDESLNRVCQ QQNRIKDLQK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 29 - 43 1714.94 1713.93 1713.91 0.02 1 AQDPSLLSNRLMIEK 82 - 88 864.60 863.59 863.43 0.16 1 QSSQKMR 93 - 116 2920.30 2919.29 2919.48 -0.19 2 TLMEETTRQQSMIRELIETNQQLK 117 - 144 3279.90 3278.90 3278.61 0.29 2 SELQLEQNRAAHQEQRANDLQQIMDSVK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3293.91 LOCUS BAB32020 170 aa linear ROD 03-APR-2004 DEFINITION unnamed protein product [Mus musculus]. ACCESSION BAB32020 VERSION BAB32020.1 GI:12860679 DBSOURCE accession AK020174.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency fulllength cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) MEDLINE 99279253 PUBMED 10349636 REFERENCE 2 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10 (10), 1617-1630 (2000) MEDLINE 20499374 PUBMED 11042159 REFERENCE 3 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai, T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa, M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka, T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M., Inoue,Y., Kira, A. and Hayashizaki,Y. TITLE RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10 (11), 1757-1771 (2000) MEDLINE 20530913 PUBMED 11076861 REFERENCE 4 AUTHORS The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 5 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 6 (residues 1 to 170) AUTHORS Adachi,J., Aizawa,K., Akahira,S., Akimura,T., Arai,A., Aono,H., Arakawa,T., Bono,H., Carninci,P., Fukuda,S., Fukunishi,Y., Furuno,M., Hanagaki,T., Hara,A., Hayatsu,N., Hiramoto,K., Hiraoka, T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Izawa,M., Kasukawa,T., Kato,H., Kawai,J., Kojima,Y., Konno,H., Kouda,M., Koya,S., Kurihara, C., Matsuyama,T., Miyazaki,A., Nishi,K., Nomura,K., Numazaki,R., Ohno,M., Okazaki,Y., Okido,T., Owa,C., Saito,H., Saito,R., Sakai, C., Sakai,K., Sano,H., Sasaki,D., Shibata,K., Shibata,Y., Shinagawa, A., Shiraki,T., Sogabe,Y., Suzuki,H., Tagami,M., Tagawa,A., Takahashi,F., Tanaka,T., Tejima,Y., Toya,T., Yamamura,T., Yasunishi, A., Yoshida,K., Yoshino,M., Muramatsu,M. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (18-AUG-2000) Yoshihide Hayashizaki, The Institute of Physical and Chemical Research (RIKEN), Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (E-mail: genome-res@gsc.riken.jp, URL:http://genome.gsc.riken.jp/, Tel:81-45- 503-9222, Fax:81-45-503-9216) COMMENT Please visit our web site (http://genome.gsc.riken.jp/) for further details. cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. First strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. Host: DH10B. FEATURES Location/Qualifiers source 1..170 /organism="Mus musculus" /strain="C57BL/6J" / db_xref="FANTOM_DB:6720484E09" /db_xref="taxon:10090" / clone="6720484E09" /sex="male" /tissue_type="wolffian duct includes surrounding region" /clone_lib="RIKEN full-length enriched mouse cDNA library" /dev_stage="12 days embryo" Protein 1..170 / name="unnamed protein product" CDS 1..170 / coded_by="AK020174.1:453..>962" /note="putative similar to SIMILAR TO P10-BINDING PROTEIN [Homo sapiens] (SPTR|Q96B31, evidence: FASTY, 77.3%ID, 72.6%length, match=462)" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦26331700 Score: 37 Expect: 14 unnamed protein product [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 62029; Calculated pI value: 9.04 NCBI BLAST search of gi¦26331700 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 19% Matched peptides shown in Bold Red 1 MDLMNGQASS VTIAATVSEG QITLMDVPVF KAIQPDELSS CGWNKKEKYS 51 SAPNAVAFTR RFNHVSFWVV REILHAQTLK IRAEVLSHYI KTAKKLYELN 101 NLHALMAVVS GLQSAPIFRL TKTWALLSRK DKTTFEKLEY VMSKEDNYKR 151 LRDYISSLKM TPCIPYLGIY LSDLTYIDSA YPSTGSILEN EQRSNLMNNI 201 LRIISDLQQS CEYDIPILPH VQKYLNSVQY IEELQKFVED DNYKLSLKIE 251 PGASTPRSAA SREDLAGPDI GASPQGGRKS SAAAAAAAAA EGALLPQTPP 301 SPRNLIPHGH RKCHSLGYNF IHKMNTAEFK SATFPNAGPR HLLDDSVMEP 351 HAPSRGQAES STLSSGISIG SSDGSELSEE TSWPAFERNR LYHSLGPVTR 401 VPRNGYRSHT KASSSAESED LAVHLYPGAV TIQGVLRRKT LLKEGKKPTV 451 ASWTKYWAAL CGTQLFYYAA KSLKATERKH FKSTSNKNVS VVGWMVMMAD 501 DPEHPDLFLL TDSEKGNSYK FQAGSRMNAM LWFKHLSAAC QSNKQQVPTN 551 LMTFE Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 194 - 202 1074.82 1073.81 1073.57 0.24 0 SNLMNNILR 245 - 257 1368.99 1367.98 1367.78 0.20 1 LSLKIEPGASTPR 249 - 278 2920.30 2919.29 2919.44 -0.15 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 408 - 438 3279.90 3278.90 3278.70 0.19 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR 472 - 478 804.58 803.57 803.45 0.12 1 SLKATER 488 - 515 3175.67 3174.67 3174.47 0.20 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 LOCUS BAC29580 555 aa linear ROD 03-APR-2004 DEFINITION unnamed protein product [Mus musculus]. ACCESSION BAC29580 VERSION BAC29580.1 GI:26331700 DBSOURCE accession AK036803.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency fulllength cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) MEDLINE 99279253 PUBMED 10349636 REFERENCE 2 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10 (10), 1617-1630 (2000) MEDLINE 20499374 PUBMED 11042159 REFERENCE 3 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai, T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa, M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka, T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M., Inoue,Y., Kira, A. and Hayashizaki,Y. TITLE RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10 (11), 1757-1771 (2000) MEDLINE 20530913 PUBMED 11076861 REFERENCE 4 AUTHORS The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 5 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 6 (residues 1 to 555) AUTHORS Adachi,J., Aizawa,K., Akimura,T., Arakawa,T., Bono,H., Carninci,P., Fukuda,S., Furuno,M., Hanagaki,T., Hara,A., Hashizume, W., Hayashida,K., Hayatsu,N., Hiramoto,K., Hiraoka,T., Hirozane,T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I., Kasukawa,T., Katoh,H., Kawai,J., Kojima,Y., Kondo,S., Konno,H., Kouda,M., Koya, S., Kurihara,C., Matsuyama,T., Miyazaki,A., Murata,M., Nakamura,M., Nishi,K., Nomura,K., Numazaki,R., Ohno,M., Ohsato,N., Okazaki,Y., Saito,R., Saitoh,H., Sakai,C., Sakai,K., Sakazume,N., Sano,H., Sasaki,D., Shibata,K., Shinagawa,A., Shiraki,T., Sogabe,Y., Tagami, M., Tagawa,A., Takahashi,F., Takaku-Akahira,S., Takeda,Y., Tanaka, T., Tomaru,A., Toya,T., Yasunishi,A., Muramatsu,M. and Hayashizaki, Y. TITLE Direct Submission JOURNAL Submitted (16-JUL-2001) Yoshihide Hayashizaki, The Institute of Physical and Chemical Research (RIKEN), Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute; 1-7- 22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (Email: genome-res@gsc.riken.jp, URL:http://genome.gsc.riken.jp/, Tel:81-45-503-9222, Fax:81-45-503-9216) COMMENT cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. Please visit our web site for further details. URL:http://genome. gsc.riken.jp/ URL:http://fantom.gsc.riken.jp/. FEATURES Location/ Qualifiers source 1..555 /organism="Mus musculus" / strain="C57BL/6J" /db_xref="FANTOM_DB:9930012L10" / db_xref="taxon:10090" /clone="9930012L10" /sex="female" / tissue_type="vagina" /clone_lib="RIKEN full-length enriched mouse cDNA library" /dev_stage="adult" Protein 1..555 /name="unnamed protein product" Region 21..248 /region_name="Guanine nucleotide exchange factor for Ras-like small GTPases" /note="RasGEF" / db_xref="CDD:14828" Region 430..543 /region_name="Pleckstrin homology domain" /note="PH" /db_xref="CDD:24224" CDS 1..555 / coded_by="AK036803.1:199..1866" /note="RAL-A EXCHANGE FACTOR RALGPS2 homolog [Mus musculus] (SPTR|Q9ERD6, evidence: FASTY, 90.1% ID, 100%length, match=1797) putative" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦12858423 Score: 37 Expect: 15 unnamed protein product [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 63029; Calculated pI value: 8.50 NCBI BLAST search of gi¦12858423 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 19% Matched peptides shown in Bold Red 1 MDLMNGQASS VTIAATVSEK SSSSESLSEK GSELKKSFDA VVFDVLKVTP 51 EEYAGQITLM DVPVFKAIQP DELSSCGWNK KEKYSSAPNA VAFTRRFNHV 101 SFWVVREILH AQTLKIRAEV LSHYIKTAKK LYELNNLHAL MAVVSGLQSA 151 PIFRLTKTWA LLSRKDKTTF EKLEYVMSKE DNYKRLRDYI SSLKMTPCIP 201 YLGIYLSDLT YIDSAYPSTG SILENEQRSN LMNNILRIIS DLQQSCEYDI 251 PILPHVQKYL NSVQYIEELQ KFVEDDNYKL SLKIEPGAST PRSAASREDL 301 AGPDIGASPQ GGRKSSAAAA AAAAAEGALL PQTPPSPRNL IPHGHRKCHS 351 LGYNFIHKMN TAEFKSATFP NAGPRHLLDD SVMEPHAPSR GQAESSTLSS 401 GISIGSSDGS ELSEETSWPA FERNRLYHSL GPVTRVPRNG YRSHTKASSS 451 AESEDLAVHL YPGAVTIQGV LRRKTLLKEG KKPTVASWTK YWAALCGTQL 501 FYYAAKSLKA TERKHFKSTS NKNVSVVGWM VMMADDPEHP DLFLLTDSEK 551 GERLDRLGSS TADPNSGS Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 229 - 237 1074.82 1073.81 1073.57 0.24 0 SNLMNNILR 280 - 292 1368.99 1367.98 1367.78 0.20 1 LSLKIEPGASTPR 284 - 313 2920.30 2919.29 2919.44 -0.15 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 443 - 473 3279.90 3278.90 3278.70 0.19 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR 507 - 513 804.58 803.57 803.45 0.12 1 SLKATER 523 - 550 3175.67 3174.67 3174.47 0.20 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 LOCUS BAB31312 568 aa linear ROD 03-APR-2004 DEFINITION unnamed protein product [Mus musculus]. ACCESSION BAB31312 VERSION BAB31312.1 GI:12858423 DBSOURCE accession AK018622.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency fulllength cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) MEDLINE 99279253 PUBMED 10349636 REFERENCE 2 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10 (10), 1617-1630 (2000) MEDLINE 20499374 PUBMED 11042159 REFERENCE 3 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai, T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa, M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka, T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M., Inoue,Y., Kira, A. and Hayashizaki,Y. TITLE RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10 (11), 1757-1771 (2000) MEDLINE 20530913 PUBMED 11076861 REFERENCE 4 AUTHORS The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 5 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 6 (residues 1 to 568) AUTHORS Adachi,J., Aizawa,K., Akahira,S., Akimura,T., Arai,A., Aono,H., Arakawa,T., Bono,H., Carninci,P., Fukuda,S., Fukunishi,Y., Furuno,M., Hanagaki,T., Hara,A., Hayatsu,N., Hiramoto,K., Hiraoka, T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Izawa,M., Kasukawa,T., Kato,H., Kawai,J., Kojima,Y., Konno,H., Kouda,M., Koya,S., Kurihara, C., Matsuyama,T., Miyazaki,A., Nishi,K., Nomura,K., Numazaki,R., Ohno,M., Okazaki,Y., Okido,T., Owa,C., Saito,H., Saito,R., Sakai, C., Sakai,K., Sano,H., Sasaki,D., Shibata,K., Shibata,Y., Shinagawa, A., Shiraki,T., Sogabe,Y., Suzuki,H., Tagami,M., Tagawa,A., Takahashi,F., Tanaka,T., Tejima,Y., Toya,T., Yamamura,T., Yasunishi, A., Yoshida,K., Yoshino,M., Muramatsu,M. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (10-JUL-2000) Yoshihide Hayashizaki, The Institute of Physical and Chemical Research (RIKEN), Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (E-mail: genome-res@gsc.riken.jp, URL:http://genome.gsc.riken.jp/, Tel:81-45- 503-9222, Fax:81-45-503-9216) COMMENT Please visit our web site (http://genome.gsc.riken.jp/) for further details. cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. First strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 185.2. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. Host: DH10B. FEATURES Location/Qualifiers source 1..568 /organism="Mus musculus" /strain="C57BL/6J" / db_xref="FANTOM_DB:9130014M22" /db_xref="taxon:10090" / clone="9130014M22" /sex="male" /tissue_type="cecum" / clone_lib="RIKEN full-length enriched mouse cDNA library" / dev_stage="adult" Protein 1..568 /name="unnamed protein product" Region 45..283 /region_name="Guanine nucleotide exchange factor for Ras-like small GTPases" /note="RasGEF" /db_xref="CDD:14828" CDS 1..568 /coded_by="AK018622.1:164..1870" /note="RAL-A EXCHANGE FACTOR RALGPS2 homolog [Mus musculus] (SPTR|Q9ERD6, evidence: FASTY, 90.1%ID, 100%length, match=1797) putative" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦11321424 Score: 36 Expect: 18 Ral-A exchange factor RalGPS2 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 65849; Calculated pI value: 8.86 NCBI BLAST search of gi¦11321424 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 18% Matched peptides shown in Bold Red 1 MDLMNGQASS VTIAATVSEK SSSSESLSEK GSELKKSFDA VVFDVLKVTP 51 EEYAGQITLM DVPVFKAIQP DELSSCGWNK KEKYSSAPNA VAFTRRFNHV 101 SFWVVREILH AQTLKIRAEV LSHYIKTAKK LYELNNLHAL MAVVSGLQSA 151 PIFRLTKTWA LLSRKDKTTF EKLEYVMSKE DNYKRLRDYI SSLKMTPCIP 201 YLGIYLSDLT YIDSAYPSTG SILENEQRSN LMNNILRIIS DLQQSCEYDI 251 PMLPHVQKYL NSVQYIEELQ KFVEDDNYKL SLKIEPGAST PRSAASREDL 301 AGPDIGASPQ GGRKSSAAAA AAAAAEGALL PQTPPSPRNL IPHGHRKCHS 351 LGYNFIHKMN TAEFKSATFP NAGPRHLLDD SVMEPHAPSR GQAESSTLSS 401 GISIGSSDGS ELSEETSWPA FERNRLYHSL GPVTRVPRNG YRSHTKASSS 451 AESEDLAVHL YPGAVTIQGV LRRKTLLKEG KKPTVASWTK YWAALCGTQL 501 FYYAAKSLKA TERKHFKSTS NKNVSVVGWM VMMADDPEHP DLFLLTDSEK 551 GNSYKFQAGS RMNAMLWFKH LSAACQSNKQ QVPTNLMTFE Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 229 - 237 1074.82 1073.81 1073.57 0.24 0 SNLMNNILR 280 - 292 1368.99 1367.98 1367.78 0.20 1 LSLKIEPGASTPR 284 - 313 2920.30 2919.29 2919.44 -0.15 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 443 - 473 3279.90 3278.90 3278.70 0.19 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR 507 - 513 804.58 803.57 803.45 0.12 1 SLKATER 523 - 550 3175.67 3174.67 3174.47 0.20 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 LOCUS AAG34162 590 aa linear ROD 23-NOV-2000 DEFINITION Ral-A exchange factor RalGPS2 [Mus musculus]. ACCESSION AAG34162 VERSION AAG34162.1 GI:11321424 DBSOURCE locus AF312924 accession AF312924.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 590) AUTHORS Martegani,E., Ceriani,M. and Bossi,D. TITLE RalGPS2, a mouse testis Ral activator JOURNAL Unpublished REFERENCE 2 (residues 1 to 590) AUTHORS Martegani,E., Ceriani,M. and Bossi,D. TITLE Direct Submission JOURNAL Submitted (13-OCT-2000) Biotecnologie e Bioscienze, Universita di Milano- Bicocca, Piazza della Scienza 2, Milano, MI 20126, Italy COMMENT Method: conceptual translation. FEATURES Location/Qualifiers source 1..590 /organism="Mus musculus" /strain="BALB/c" / db_xref="taxon:10090" /sex="male" /tissue_type="testis" / dev_stage="9-11 weeks old" Protein 1..590 /product="Ral-A exchange factor RalGPS2" /note="contains a CDC25 exchange domain and a PH domain" Region 45..283 /region_name="Guanine nucleotide exchange factor for Ras-like small GTPases" /note="RasGEF" / db_xref="CDD:14828" Region 465..578 /region_name="Pleckstrin homology domain" /note="PH" /db_xref="CDD:24224" CDS 1..590 / coded_by="AF312924.1:70..1842" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦31560036 Score: 36 Expect: 18 Ral-A exchange factor RalGPS2 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 65845; Calculated pI value: 8.86 NCBI BLAST search of gi¦31560036 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦26329225 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 18% Matched peptides shown in Bold Red 1 MDLMNGQASS VTIAATVSEK SSSSESLSEK GSELKKSFDA VVFDVLKVTP 51 EEYAGQITLM DVPVFKAIQP DELSSCGWNK KEKYSSAPNA VAFTRRFNHV 101 SFWVVREILH AQTLKIRAEV LSHYIKTAKK LYELNNLHAL MAVVSGLQSA 151 PIFRLTKTWA LLSRKDKTTF EKLEYVMSKE DNYKRLRDYI SSLKMTPCIP 201 YLGIYLSDLT YIDSAYPSTG SILENEQRSN LMNNILRIIS DLQQSCEYDI 251 PILPHVQKYL NSVQYIEELQ KFVEDDNYKL SLKIEPGAST PRSAASREDL 301 AGPDIGASPQ GGRKSSAAAA AAAAAEGALL PQTPPSPRNL IPHGHRKCHS 351 LGYNFIHKMN TAEFKSATFP NAGPRHLLDD SVMEPHAPSR GQAESSTLSS 401 GISIGSSDGS ELSEETSWPA FERNRLYHTL GPVTRVPRNG YRSHTKASSS 451 AESEDLAVHL YPGAVTIQGV LRRKTLLKEG KKPTVASWTK YWAALCGTQL 501 FYYAAKSLKA TERKHFKSTS NKNVSVVGWM VMMADDPEHP DLFLLTDSEK 551 GNSYKFQAGS RMNAMLWFKH LSAACQSNKQ QVPTNLMTFE Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 229 - 237 1074.82 1073.81 1073.57 0.24 0 SNLMNNILR 280 - 292 1368.99 1367.98 1367.78 0.20 1 LSLKIEPGASTPR 284 - 313 2920.30 2919.29 2919.44 -0.15 2 IEPGASTPRSAASREDLAGPDIGASPQGGR 443 - 473 3279.90 3278.90 3278.70 0.19 2 SHTKASSSAESEDLAVHLYPGAVTIQGVLRR 507 - 513 804.58 803.57 803.45 0.12 1 SLKATER 523 - 550 3175.67 3174.67 3174.47 0.20 0 NVSVVGWMVMMADDPEHPDLFLLTDSEK No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3293.91 LOCUS NP_076373 590 aa linear ROD 25-AUG-2004 DEFINITION Ral-A exchange factor RalGPS2 [Mus musculus]. ACCESSION NP_076373 VERSION NP_076373.2 GI:31560036 DBSOURCE REFSEQ: accession NM_023884.2 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 590) AUTHORS Martegani,E., Ceriani,M., Tisi,R. and Berruti,G. TITLE Cloning and characterization of a new Ral-GEF expressed in mouse testis JOURNAL Ann. N. Y. Acad. Sci. 973, 135-137 (2002) PUBMED 12485849 REFERENCE 2 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 3 AUTHORS The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 4 (residues 1 to 590) AUTHORS Rebhun,J.F., Chen,H. and Quilliam,L.A. TITLE Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral JOURNAL J. Biol. Chem. 275 (18), 13406-13410 (2000) PUBMED 10747847 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK033549.1. On Jun 10, 2003 this sequence version replaced gi:13357218. FEATURES Location/ Qualifiers source 1..590 /organism="Mus musculus" / strain="C57BL/6J" /db_xref="taxon:10090" /chromosome="1" Protein 1..590 /product="Ral-A exchange factor RalGPS2" Region 45..283 / region_name="Guanine nucleotide exchange factor for Ras-like small GTPases" /note="RasGEF" /db_xref="CDD:14828" Region 465..578 / region_name="Pleckstrin homology domain" /note="PH" / db_xref="CDD:24224" CDS 1..590 /gene="Ralgps2" / coded_by="NM_023884.2:118..1890" /note="go_function: guanylnucleotide exchange factor activity [goid 0005085] [evidence ISS] [pmid 12466851]; go_process: intracellular signaling cascade [goid 0007242] [evidence ISS] [pmid 12466851]" /db_xref="GeneID:78255" / db_xref="LocusID:78255" /db_xref="MGI:1925505" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦28570774 Score: 28 Expect: 1.1e+002 immunoglobulin heavy chain [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 3435; Calculated pI value: 9.63 NCBI BLAST search of gi¦28570774 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 2 Sequence Coverage: 100% Matched peptides shown in Bold Red 1 CARKGGRNHS RSYPYAMDYW GQGTSVTVSS G Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 1 - 7 804.58 803.57 803.42 0.15 2 CARKGGR 5 - 31 2920.30 2919.29 2919.30 -0.01 2 GGRNHSRSYPYAMDYWGQGTSVTVSSG No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAO40559 31 aa linear ROD 27-FEB-2003 DEFINITION immunoglobulin heavy chain [Mus musculus]. ACCESSION AAO40559 VERSION AAO40559.1 GI:28570774 DBSOURCE accession AY205817.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 31) AUTHORS Ippolito,G.C., Zemlin,M., Ivanov,I., Nguyen,H.H., Warr,I., Schelonka,R.L., Link,J.M., Zemlin, C., Nitschke,L., Pelkonen,J., Marion,T., Mestecky,J., Rajewsky,K. and Schroeder,H.W. Jr. TITLE Immune deficiency and autoimmunity in an immunoglobulin D-limited mouse JOURNAL Unpublished REFERENCE 2 (residues 1 to 31) AUTHORS Ippolito,G.C., Zemlin,M., Ivanov,I., Nguyen,H.H., Warr,I., Schelonka,R.L., Link,J.M., Zemlin,C., Nitschke,L., Pelkonen,J., Marion,T., Mestecky,J., Rajewsky,K. and Schroeder,H.W. Jr. TITLE Direct Submission JOURNAL Submitted (23- DEC-2002) Departments of Microbiology, Medicine, and Pediatrics, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, AL 35294-3300, USA COMMENT Method: conceptual translation. FEATURES Location/Qualifiers source 1..31 / organism="Mus musculus" /strain="BALB/cJ" /db_xref="taxon:10090" / clone="1F-19" /cell_type="B lymphocyte" /tissue_type="bone marrow" / note="B220+,IgM+,IgD+ D-limited" Protein <1..>31 / product="immunoglobulin heavy chain" CDS 1..31 /gene="Igh" / coded_by="AY205817.1:<1..>93" /note="complementarity determining region 3" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦13508539 Score: 37 Expect: 16 CLASP2 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 67012; Calculated pI value: 6.24 NCBI BLAST search of gi¦13508539 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 18% Matched peptides shown in Bold Red 1 MEPRGAEYFC AQVLQKDVSG RLQAGEELLL CLGTPGAIPD LEDDPSRLAK 51 TVDALTRWVG SSNYRVSLLG LEILSAFVDR LSTRFKSYVT MVTTALIDRM 101 GDVKDKVREE AQNLTLKLMD EVAPPMYIWE QLASGFKHKN FRSREGVCLC 151 LIETLNIFGT QPLVISKLVP HLCVLFGDSN SQVRNAALSA VVEIYRHVGE 201 KLRIDLCKRD IPPARLEMVL AKFDEVQNSG GMILSVCKDK SFDDEESVDG 251 NRPSSAASAF KVPAPKTPGN PVSSARKPGS AGGPKVGGPS KEGGAGAVDE 301 DDFIKAFTDV PSVQIYSSRE LEETLNKIRE ILSDDKHDWD QRANALKKIR 351 SLLVAGAAQY DCFFQHLRLL KELSNHNERI EERKIALYEL MKLTQEESFS 401 VWDEHFKTIL LLLLETLGDK EPTIRALALK VLKEILRHQP ARFKNYAELT 451 VMKTLEAHKD PHKEVVRSAE EAASVLATSI SPEQCIKVLC PIIQTADYPI 501 NLAAIKMQTK VIERVSKETL NMLLPEIMPG LIQGYDNSES SVRKACVFCL 551 VAVHAVIGDE LKPHLSQLTG SKMKLLNLYI KRAQTGSAGA DPTADVSGQS 601 Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 17 - 47 3293.91 3292.90 3292.62 0.27 1 DVSGRLQAGEELLLCLGTPGAIPDLEDDPSR 204 - 209 804.58 803.57 803.43 0.14 1 IDLCKR 223 - 240 2026.92 2025.92 2025.95 -0.03 1 FDEVQNSGGMILSVCKDK 239 - 266 2951.69 2950.69 2950.39 0.29 2 DKSFDDEESVDGNRPSSAASAFKVPAPK 369 - 379 1353.00 1352.00 1351.72 0.27 1 LLKELSNHNER 372 - 384 1653.96 1652.95 1652.82 0.13 2 ELSNHNERIEERK 431 - 442 1459.93 1458.92 1458.88 0.04 2 VLKEILRHQPAR No match to: 727.74, 749.74, 776.64, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1368.99, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90 LOCUS CAC35161 600 aa linear ROD 26-MAR-2001 DEFINITION CLASP2 [Mus musculus]. ACCESSION CAC35161 VERSION CAC35161.1 GI:13508539 DBSOURCE embl locus MMU276961, accession AJ276961.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Akhmanova,A., Hoogenraad,C.C., Drabek,K., Stepanova,T., Dortland,B., Verkerk,T., Vermeulen,W., Burgering,B.M., de Zeeuw,C. I., Grosveld,F. and Galjar,N. TITLE Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts JOURNAL Cell 104 (6), 923-935 (2001) MEDLINE 21185938 PUBMED 11290329 REFERENCE 2 (residues 1 to 600) AUTHORS Galjart,N. TITLE Direct Submission JOURNAL Submitted (30-MAR-2000) Galjart N., Cell Biology and Genetics, Erasmus University Rotterdam, P.O. Box 1738, 3000 DR, NETHERLANDS COMMENT related sequence AA251672. FEATURES Location/ Qualifiers source 1..600 /organism="Mus musculus" / db_xref="taxon:10090" Protein 1..600 /product="CLASP2" Region 320.. >525 /region_name="Adaptin N terminal region" /note="Adaptin_N" / db_xref="CDD:25788" Region 452..585 /region_name="Mast C-terminus" / note="Mast_C" /db_xref="CDD:27035" CDS 1..600 /gene="Clasp2" / coded_by="AJ276961.1:209..2011" /db_xref="GOA:Q99JI3" / db_xref="UniProt/TrEMBL:Q99JI3" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦1166506 Score: 37 Expect: 16 Stat3B Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 83772; Calculated pI value: 6.70 NCBI BLAST search of gi¦1166506 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 14% Matched peptides shown in Bold Red 1 MAQWNQLQQL DTRYLEQLHQ LYSDTFPMEL RQFLAPWIES QDWAYAASKE 51 SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME 101 IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD 151 VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM 201 QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY VQKTLTDEEL ADWKRRQQIA 251 CIGGPPNICL DRLENWITSL AESQLQTRQQ IKKLEELQQK VSYKGDPIVQ 301 HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTTKVR 351 LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN 401 NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL 451 KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT 501 WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITWAKFC 551 KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST 601 KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF 651 AEIIMGYKIM DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHPEADPG 701 SAAPYLKTKF ICVTPFIDAV WK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 309 - 314 776.64 775.63 775.46 0.17 0 IVELFR 349 - 354 727.74 726.73 726.51 0.22 1 VRLLVK 393 - 417 2920.30 2919.29 2919.40 -0.10 2 VMNMEESNNGSLSAEFKHLTLREQR 410 - 423 1653.96 1652.95 1652.83 0.12 2 HLTLREQRCGNGGR 616 - 626 1252.83 1251.82 1251.61 0.21 0 EGGVTFTWVEK 632 - 658 3161.64 3160.63 3160.56 0.07 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK 686 - 707 2502.02 2501.02 2501.13 -0.11 0 YCRPESQEHPEADPGSAAPYLK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 LOCUS AAC52612 722 aa linear ROD 17-JUN-1996 DEFINITION Stat3B. ACCESSION AAC52612 VERSION AAC52612.1 GI:1166506 DBSOURCE locus MMU30709 accession U30709.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 722) AUTHORS Schaefer,T.S., Sanders,L.K. and Nathans,D. TITLE Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 92 (20), 9097- 9101 (1995) MEDLINE 96016116 PUBMED 7568080 REFERENCE 2 (residues 1 to 722) AUTHORS Caldenhoven,E., van Dijk,T.B., Solari,R., Armstrong, J., Raaijmakers,J.A., Lammers,J.W., Koenderman,L. and de Groot,R.P. TITLE STAT3beta, a splice variant of transcription factor STAT3, is a dominant negative regulator of transcription JOURNAL J. Biol. Chem. 271 (22), 13221-13227 (1996) MEDLINE 96278730 PUBMED 8675499 REFERENCE 3 (residues 1 to 722) AUTHORS Schaefer,T.S., Sanders,L.K. and Nathans,D. TITLE Direct Submission JOURNAL Submitted (30-JUN- 1995) Timothy S. Schaefer, Mol. Biol & Genetics, Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205, USA FEATURES Location/Qualifiers source 1..722 / organism="Mus musculus" /db_xref="taxon:10090" Protein 1..722 / product="Stat3B" Region 2..116 /region_name="STAT protein, protein interaction domain" /note="STAT_int" /db_xref="CDD:23390" Region 138..319 /region_name="STAT protein, all-alpha domain" / note="STAT_alpha" /db_xref="CDD:1568" Region 321..574 / region_name="STAT protein, DNA binding domain" /note="STAT_bind" / db_xref="CDD:8403" Region 583..674 /region_name="Src homology 2 domains" /note="SH2" /db_xref="CDD:16538" CDS 1..722 / coded_by="U30709.1:173..2341" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦17512414 Score: 37 Expect: 16 Signal transducer and activator of transcription 3, isoform 3 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 83758; Calculated pI value: 6.70 NCBI BLAST search of gi¦17512414 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦47458820 from Homo sapiens gi¦22094115 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 14% Matched peptides shown in Bold Red 1 MAQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAYAASKE 51 SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME 101 IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD 151 VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM 201 QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY VQKTLTDEEL ADWKRRQQIA 251 CIGGPPNICL DRLENWITSL AESQLQTRQQ IKKLEELQQK VSYKGDPIVQ 301 HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTTKVR 351 LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN 401 NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL 451 KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT 501 WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITWAKFC 551 KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST 601 KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF 651 AEIIMGYKIM DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHPEADPG 701 SAAPYLKTKF ICVTPFIDAV WK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 309 - 314 776.64 775.63 775.46 0.17 0 IVELFR 349 - 354 727.74 726.73 726.51 0.22 1 VRLLVK 393 - 417 2920.30 2919.29 2919.40 -0.10 2 VMNMEESNNGSLSAEFKHLTLREQR 410 - 423 1653.96 1652.95 1652.83 0.12 2 HLTLREQRCGNGGR 616 - 626 1252.83 1251.82 1251.61 0.21 0 EGGVTFTWVEK 632 - 658 3161.64 3160.63 3160.56 0.07 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK 686 - 707 2502.02 2501.02 2501.13 -0.11 0 YCRPESQEHPEADPGSAAPYLK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 LOCUS AAH19168 722 aa linear ROD 29-JUN-2004 DEFINITION Signal transducer and activator of transcription 3, isoform 3 [Mus musculus]. ACCESSION AAH19168 VERSION AAH19168.1 GI:17512414 DBSOURCE accession BC019168.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 722) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J. G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G. D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N. K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T. E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange, C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy, S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne, P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs, R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M. C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 722) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (07-DEC-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey E. Green, M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen, N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q. L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell, J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon, C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 39 Row: c Column: 12 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 24475592. Method: conceptual translation. FEATURES Location/Qualifiers source 1..722 /organism="Mus musculus" / strain="FVB/N" /db_xref="taxon:10090" /clone="MGC:29120 IMAGE:4923137" /tissue_type="Salivary gland, 10 week old female mouse" /clone_lib="NCI_CGAP_SG2" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..722 /product="signal transducer and activator of transcription 3, isoform 3" Region 2..116 / region_name="STAT protein, protein interaction domain" / note="STAT_int" /db_xref="CDD:23390" Region 138..319 / region_name="STAT protein, all-alpha domain" /note="STAT_alpha" / db_xref="CDD:1568" Region 321..574 /region_name="STAT protein, DNA binding domain" /note="STAT_bind" /db_xref="CDD:8403" Region 583..674 /region_name="Src homology 2 domains" /note="SH2" / db_xref="CDD:16538" CDS 1..722 /gene="Stat3" / coded_by="BC019168.1:184..2352" /db_xref="LocusID:20848" / db_xref="MGI:103038" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦458706 Score: 35 Expect: 24 Stat3 Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 88809; Calculated pI value: 6.10 NCBI BLAST search of gi¦458706 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 13% Matched peptides shown in Bold Red 1 MAQWNQLQQL DTRYLKQLHQ LYSDTFPMEL RQFLAPWIES QDWAYAASKE 51 SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME 101 IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD 151 VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM 201 QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY VQKTLTDEEL ADWKRRQQIA 251 CIGGPPNICL DRLENWITSL AESQLQTRQQ IKKLEELQQK VSYKGDPIVQ 301 HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTTKVR 351 LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN 401 NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL 451 KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT 501 WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITWAKFC 551 KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST 601 KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF 651 AEIIMGYKIM DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHPEADPG 701 SAAPYLKTKF ICVTPTTCSN TIDLPMSPRT LDSLMQFGNN GEGAEPSAGG 751 QFESLTFDMD LTSECATSPM Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 309 - 314 776.64 775.63 775.46 0.17 0 IVELFR 349 - 354 727.74 726.73 726.51 0.22 1 VRLLVK 393 - 417 2920.30 2919.29 2919.40 -0.10 2 VMNMEESNNGSLSAEFKHLTLREQR 410 - 423 1653.96 1652.95 1652.83 0.12 2 HLTLREQRCGNGGR 616 - 626 1252.83 1251.82 1251.61 0.21 0 EGGVTFTWVEK 632 - 658 3161.64 3160.63 3160.56 0.07 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK 686 - 707 2502.02 2501.02 2501.13 -0.11 0 YCRPESQEHPEADPGSAAPYLK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 LOCUS AAA19452 770 aa linear ROD 01-JUL-1994 DEFINITION Stat3. ACCESSION AAA19452 VERSION AAA19452.1 GI:458706 DBSOURCE locus MMU06922 accession U06922.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 770) AUTHORS Zhong,Z., Wen,Z. and Darnell,J.E. Jr. TITLE Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6 JOURNAL Science 264 (5155), 95-98 (1994) MEDLINE 94188718 PUBMED 8140422 REFERENCE 2 (residues 1 to 770) AUTHORS Zhong,Z., Wen,Z. and Darnell,J.E. Jr. TITLE Stat3 and Stat4: members of the family of signal transducers and activators of transcription JOURNAL Proc. Natl. Acad. Sci. U.S.A. 91 (11), 4806-4810 (1994) MEDLINE 94255416 PUBMED 7545930 REFERENCE 3 (residues 1 to 770) AUTHORS Zhong,Z. TITLE Direct Submission JOURNAL Submitted (18-FEB-1994) Z. Zhong, The Rockefeller University, The Molecular Cell Biology Laboratory, 1230 York Avenue, New York, NY 10021, USA COMMENT Method: conceptual translation. FEATURES Location/Qualifiers source 1..770 / organism="Mus musculus" /db_xref="taxon:10090" /sex="female" / tissue_type="thymus" /clone_lib="Mouse thymus cDNA library (Stratagene)" /dev_stage="6-8 weeks" Protein 1..770 / product="Stat3" /function="DNA binding protein induced by EGF and IL-6" Region 2..116 /region_name="STAT protein, protein interaction domain" /note="STAT_int" /db_xref="CDD:23390" Region 138..319 / region_name="STAT protein, all-alpha domain" /note="STAT_alpha" / db_xref="CDD:1568" Region 321..574 /region_name="STAT protein, DNA binding domain" /note="STAT_bind" /db_xref="CDD:8403" Region 583..674 /region_name="Src homology 2 domains" /note="SH2" / db_xref="CDD:16538" CDS 1..770 /gene="Stat3" / coded_by="U06922.1:69..2381" /citation=[1] /citation=[2] Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦34559410 Score: 35 Expect: 24 signal transducer and activator of transcription 3 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 88796; Calculated pI value: 5.94 NCBI BLAST search of gi¦34559410 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦34559408 from Mus musculus gi¦13277852 from Mus musculus gi¦47458804 from Mus musculus gi¦1711553 from Mus musculus gi¦18087726 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 13% Matched peptides shown in Bold Red 1 MAQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAYAASKE 51 SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME 101 IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD 151 VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM 201 QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY VQKTLTDEEL ADWKRRQQIA 251 CIGGPPNICL DRLENWITSL AESQLQTRQQ IKKLEELQQK VSYKGDPIVQ 301 HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTTKVR 351 LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN 401 NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL 451 KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT 501 WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITWAKFC 551 KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST 601 KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF 651 AEIIMGYKIM DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHPEADPG 701 SAAPYLKTKF ICVTPTTCSN TIDLPMSPRT LDSLMQFGNN GEGAEPSAGG 751 QFESLTFDMD LTSECATSPM Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 309 - 314 776.64 775.63 775.46 0.17 0 IVELFR 349 - 354 727.74 726.73 726.51 0.22 1 VRLLVK 393 - 417 2920.30 2919.29 2919.40 -0.10 2 VMNMEESNNGSLSAEFKHLTLREQR 410 - 423 1653.96 1652.95 1652.83 0.12 2 HLTLREQRCGNGGR 616 - 626 1252.83 1251.82 1251.61 0.21 0 EGGVTFTWVEK 632 - 658 3161.64 3160.63 3160.56 0.07 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK 686 - 707 2502.02 2501.02 2501.13 -0.11 0 YCRPESQEHPEADPGSAAPYLK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 LOCUS AAQ75419 770 aa linear ROD 01-DEC-2003 DEFINITION signal transducer and activator of transcription 3 [Mus musculus]. ACCESSION AAQ75419 VERSION AAQ75419.1 GI:34559410 DBSOURCE accession AY299490.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 770) AUTHORS Davoodi-Semiromi,A. and She,J.-X. TITLE A Mutant Stat5b with Weaker DNA Binding Defines a Key Defective Pathway in Non-Obese Diabetic (NOD) Mice JOURNAL Unpublished REFERENCE 2 (residues 1 to 770) AUTHORS Davoodi-Semiromi,A. and She,J.-X. TITLE Direct Submission JOURNAL Submitted (15-MAY-2003) Center for Biotechnology and Genomic Medicine, Medical College of Georgia, 1120 15th Street, PV6B108, Augusta, GA, USA FEATURES Location/ Qualifiers source 1..770 /organism="Mus musculus" /strain="NOD/ LtJ" /db_xref="taxon:10090" /chromosome="11" Protein 1..770 / product="signal transducer and activator of transcription 3" / name="transcription factor" Region 2..116 /region_name="STAT protein, protein interaction domain" /note="STAT_int" / db_xref="CDD:23390" Region 138..319 /region_name="STAT protein, allalpha domain" /note="STAT_alpha" /db_xref="CDD:1568" Region 321..574 /region_name="STAT protein, DNA binding domain" / note="STAT_bind" /db_xref="CDD:8403" Region 583..674 / region_name="Src homology 2 domains" /note="SH2" / db_xref="CDD:16538" CDS 1..770 /gene="Stat3" / coded_by="AY299490.1:7..2319" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦2137460 Score: 35 Expect: 25 ISGF3 p91-related transcription factor - mouse Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 88778; Calculated pI value: 5.94 NCBI BLAST search of gi¦2137460 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦476716 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 13% Matched peptides shown in Bold Red 1 MAQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAYAASKE 51 SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME 101 IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD 151 VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM 201 QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY VQKTLTDEEL ADWKRRQQIA 251 CIGGPPNICL DRLENWITSL AESQLQTRQQ IKKLEELQQK VSYKGDPIVQ 301 HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTTKVR 351 LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVINMEESN 401 NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL 451 KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT 501 WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITWAKFC 551 KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST 601 KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF 651 AEIIMGYKIM DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHPEADPG 701 SAAPYLKTKF ICVTPTTCSN TIDLPMSPRT LDSLMQFGNN GEGAEPSAGG 751 QFESLTFDMD LTSECATSPM Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 295 - 318 2920.30 2919.29 2919.56 -0.27 2 GDPIVQHRPMLEERIVELFRNLMK 309 - 314 776.64 775.63 775.46 0.17 0 IVELFR 349 - 354 727.74 726.73 726.51 0.22 1 VRLLVK 410 - 423 1653.96 1652.95 1652.83 0.12 2 HLTLREQRCGNGGR 616 - 626 1252.83 1251.82 1251.61 0.21 0 EGGVTFTWVEK 632 - 658 3161.64 3160.63 3160.56 0.07 1 TQIQSVEPYTKQQLNNMSFAEIIMGYK 686 - 707 2502.02 2501.02 2501.13 -0.11 0 YCRPESQEHPEADPGSAAPYLK No match to: 749.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 LOCUS I49508 770 aa linear ROD 01-DEC-2000 DEFINITION ISGF3 p91- related transcription factor - mouse. ACCESSION I49508 VERSION I49508 GI:2137460 DBSOURCE pir: locus I49508; summary: #length 770 #molecular-weight 88035 #checksum 6757 ; genetic: #gene APRF ; superfamily: human signal transducer and transcription activator STAT5A ; PIR dates: 02-Jul-1996 #sequence_revision 02-Jul-1996 #text_change 01-Dec-2000 . KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 770) AUTHORS Akira,S., Nishio,Y., Inoue,M., Wang,X.J., Wei,S., Matsusaka,T., Yoshida,K., Sudo,T., Naruto,M. and Kishimoto,T. TITLE Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91- related transcription factor involved in the gp130-mediated signaling pathway JOURNAL Cell 77 (1), 63-71 (1994) MEDLINE 94208062 PUBMED 7512451 REFERENCE 2 (residues 1 to 770) AUTHORS Raz, R., Durbin,J.E. and Levy,D.E. TITLE Acute phase response factor and additional members of the interferon-stimulated gene factor 3 family integrate diverse signals from cytokines, interferons, and growth factors JOURNAL J. Biol. Chem. 269 (39), 24391-24395 (1994) MEDLINE 95014185 PUBMED 7523373 FEATURES Location/Qualifiers source 1..770 /organism="Mus musculus" /db_xref="taxon:10090" Protein 1..770 /product="ISGF3 p91-related transcription factor" Region 2..116 /region_name="STAT protein, protein interaction domain" / note="STAT_int" /db_xref="CDD:23390" Region 138..319 / region_name="STAT protein, all-alpha domain" /note="STAT_alpha" / db_xref="CDD:1568" Region 321..574 /region_name="STAT protein, DNA binding domain" /note="STAT_bind" /db_xref="CDD:8403" Region 583..674 /region_name="Src homology 2 domains" /note="SH2" / db_xref="CDD:16538" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦2137361 Score: 36 Expect: 18 GPI-anchored protein - mouse (fragment) Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 35986; Calculated pI value: 4.70 NCBI BLAST search of gi¦2137361 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦902342 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 24% Matched peptides shown in Bold Red 1 MKQILGVIDK KLRNLEKKKG KLDDYQERMN KGERLNQDQL DAVSKYQEVT 51 NNLEFAKELQ RSFMALSQDI QKTIKKTARR EQLMREEAEQ KRLKTVLELQ 101 YVLDKLGDDD VRTDLKQGLS GVPILSEEEL SLLDEFYKLV DPERDMSLRL 151 NEQYEHASIH LWDLLEGKEK PVCGTTYKAL KEIVERVFQS NYFDSTHNHQ 201 NGLCEEEEAA SAPTVEDQVA EAEPEPAEEY TEQSEVESTE YVNRQFMAET 251 QFSSGEKEQV DEWTVETVEV VNSLQQQPQA ASPSVPEPHS LTPVAQSDPL 301 VRRQRVQDLM A Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 1 - 10 1144.83 1143.82 1143.67 0.15 1 MKQILGVIDK 22 - 34 1653.96 1652.95 1652.76 0.19 2 LDDYQERMNKGER 117 - 144 3175.67 3174.67 3174.63 0.03 1 QGLSGVPILSEEELSLLDEFYKLVDPER 139 - 149 1330.83 1329.82 1329.67 0.15 1 LVDPERDMSLR 150 - 168 2294.95 2293.95 2294.13 -0.19 0 LNEQYEHASIHLWDLLEGK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1171.90, 1252.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3279.90, 3293.91 LOCUS S58008 311 aa linear ROD 05-NOV-1999 DEFINITION GPI-anchored protein - mouse (fragment). ACCESSION S58008 VERSION S58008 GI:2137361 DBSOURCE pir: locus S58008; summary: #length 311 #checksum 5296 ; PIR dates: 13-Jan-1996 #sequence_revision 01-Mar- 1996 #text_change 05-Nov-1999 ; punctuation in sequence. KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 311) AUTHORS Gessler,M. TITLE Direct Submission JOURNAL Submitted (??-JUL-1995) to the EMBL Data Library FEATURES Location/Qualifiers source 1..311 /organism="Mus musculus" / db_xref="taxon:10090" Protein 1..311 /product="GPI-anchored protein" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦13529410 Score: 35 Expect: 24 Nf2 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 41732; Calculated pI value: 8.64 NCBI BLAST search of gi¦13529410 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 26% Matched peptides shown in Bold Red 1 MAGAIASRMS FSSLKRKQPK TFTVRIVTMD AEMEFNCEMK WKGKDLFDLV 51 CRTLGLRETW FFGLQYTIKD TVAWLKMDKK VLDHDVSKEE PVTFHFLAKF 101 YPENAEEELV QEITQHLFFL QVKKQILDEK VYCPPEASVL LASYAVQAKY 151 GDYDPSVHKR GFLAQEELLP KRVINLYQMT PEMWEERITA WYAEHRGRAR 201 DEAEMEYLKI AQDLEMYGVN YFTIRNKKGT ELLLGVDALG LHIYDPENRL 251 TPKISFLWNE IRNISYSDKE FTIKPLDKKI DVFKFNSSKL RVNKLILQLC 301 IGNHDLFMRR RKADSLEVQQ MKAQAREERE RERGRGGERE RARMGPQNVS 351 SLL Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 1 - 8 776.64 775.63 775.40 0.23 0 MAGAIASR 100 - 123 2951.69 2950.69 2950.48 0.21 0 FYPENAEEELVQEITQHLFFLQVK 124 - 149 2920.30 2919.29 2919.54 -0.25 2 KQILDEKVYCPPEASVLLASYAVQAK 229 - 249 2294.95 2293.95 2294.19 -0.25 0 GTELLLGVDALGLHIYDPENR 279 - 284 749.74 748.73 748.45 0.28 1 KIDVFK 323 - 331 1144.83 1143.82 1143.57 0.25 2 AQAREERER No match to: 727.74, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2338.05, 2502.02, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH05442 353 aa linear ROD 13-FEB-2004 DEFINITION Nf2 protein [Mus musculus]. ACCESSION AAH05442 VERSION AAH05442.1 GI:13529410 DBSOURCE accession BC005442.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 353) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J. G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G. D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N. K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T. E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange, C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy, S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne, P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs, R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M. C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 353) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (27-MAR-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Lothar Hennighausen Ph.D., Robin Humphreys cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http://www-shgc. stanford.edu Contact: (Dickson, Mark) mcd@paxil.stanford.edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl. gov Series: IRAK Plate: 8 Row: d Column: 8 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 6754827. Method: conceptual translation. FEATURES Location/Qualifiers source 1..353 / organism="Mus musculus" /strain="mix FVB/N, C57BL/6J" / db_xref="taxon:10090" /clone="MGC:6102 IMAGE:3496053" / tissue_type="Mammary tumor. WAP-TGF alpha model. 7 months old, gross tissue." /clone_lib="NCI_CGAP_Mam5" /lab_host="DH10B" / note="Vector: pCMV-SPORT6" Protein 1..353 /product="Nf2 protein" Region 18..222 /region_name="Band 4" /note="B41" / db_xref="CDD:24244" Region 24..222 /region_name="FERM domain (Band 4.1 family). This domain has been renamed the FERM domain, which stands for F for 4.1, E for Ezrin, R for radixin and M for moesin" / note="Band_41" /db_xref="CDD:pfam00373" Region 226..>324 / region_name="Ezrin/radixin/moesin family" /note="ERM" / db_xref="CDD:24470" Region 226..324 /region_name="Ezrin/radixin/ moesin family. This family of proteins contain a band 4.1 domain (pfam00373), at their amino terminus. This family represents the rest of these proteins" /note="ERM" /db_xref="CDD:pfam00769" CDS 1..353 /gene="Nf2" /coded_by="BC005442.1:426..1487" / db_xref="LocusID:18016" /db_xref="MGI:97307" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦2598562 Score: 34 Expect: 27 BiP [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 72547; Calculated pI value: 5.10 NCBI BLAST search of gi¦2598562 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 19% Matched peptides shown in Bold Red 1 MMKFTVVAAA LLLLGAVRAE EEDKKEDVGT VVGIDLGTTY SCVGVFKNGR 51 VEIIANDQGN RITPSYVAFT PEGERLIGDA AKNQLTSNPE NTVFDAKRLI 101 GRTWNDPSVQ QDIKFLPFKV VEKKTKPYIQ VDIGGGQTKT FAPEEISAMV 151 LTKMKETAEA YLGKKVTHAV VTVPAYFNDA QRQATKDAGT IAGLNVMRII 201 NEPTAAAIAY GLDKREGEKN ILVFDLGGGT FDVSLLTIDN GVFEVVATNG 251 DTHLGGEDFD QRVMEHFIKL YKKKTGKDVR KDNRAVQKLR REVEKAKRAL 301 SSQHQARIEI ESFFEGEDFS ETLTRAKFEE LNMDLFRSTM KPVQKVLEDS 351 DLKKSDIDEI VLVGGSTRIP KIQQLVKEFF NGKEPSRGIN PDEAVAYGAA 401 VQAGVLSGDQ DTGDLVLLDV CPLTLGIETV GGVMTKLIPR NTVVPTKKSQ 451 IFSTASDNQP TVTIKVYEGE RPLRKDNHLL GTFDLTGIPP APRGVPQIEV 501 TFEIDVNGIL RVTAEDKGTG NKNKITITND QNRLTPEEIE RMVNDAEKFA 551 EEDKKLKERI DTRNELESYA YSLKNQIGDK EKLGGKLSSE DKETMEKAVE 601 EKIEWLESHQ DADIEDFKAK KKELEEIVQP IISKLYGSGG PPPTGEEDTS 651 EKDEL Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 19 - 47 3145.63 3144.62 3144.52 0.10 2 AEEEDKKEDVGTVVGIDLGTTYSCVGVFK 76 - 98 2502.02 2501.02 2501.29 -0.27 2 LIGDAAKNQLTSNPENTVFDAKR 154 - 165 1368.99 1367.98 1367.71 0.27 2 MKETAEAYLGKK 296 - 307 1353.00 1352.00 1351.74 0.25 2 AKRALSSQHQAR 466 - 493 3161.64 3160.63 3160.68 -0.05 2 VYEGERPLRKDNHLLGTFDLTGIPPAPR 525 - 533 1074.82 1073.81 1073.55 0.26 0 ITITNDQNR 621 - 634 1653.96 1652.95 1652.97 -0.02 2 KKELEEIVQPIISK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2920.30, 2951.69, 3070.66, 3131.63, 3175.67, 3279.90, 3293.91 LOCUS CAA05361 655 aa linear ROD 07-NOV-1997 DEFINITION BiP [Mus musculus]. ACCESSION CAA05361 VERSION CAA05361.1 GI:2598562 DBSOURCE embl locus MMBIPCHAP, accession AJ002387.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Haas,I.G. and Meo,T. TITLE cDNA cloning of the immunoglobulin heavy chain binding protein JOURNAL Proc. Natl. Acad. Sci. U.S.A. 85 (7), 2250-2254 (1988) MEDLINE 88176922 PUBMED 2895472 REFERENCE 2 (residues 1 to 655) AUTHORS Haas,I.G. TITLE Direct Submission JOURNAL Submitted (03-NOV-1997) Haas I.G., Biochemiezentrum Heidelberg (BZH), University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, GERMANY FEATURES Location/ Qualifiers source 1..655 /organism="Mus musculus" / db_xref="taxon:10090" /tissue_lib="liver" Protein 1..655 / product="BiP" /function="chaperone" Region 31..636 / region_name="Hsp70 protein" /note="HSP70" /db_xref="CDD:4036" CDS 1..655 /coded_by="AJ002387.1:12..1979" /db_xref="GOA:P20029" / db_xref="UniProt/Swiss-Prot:P20029" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦41946803 Score: 34 Expect: 28 RIKEN cDNA 2900024D24 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 32879; Calculated pI value: 8.95 NCBI BLAST search of gi¦41946803 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦42734483 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 31% Matched peptides shown in Bold Red 1 MPLHQISVIP ARETASNGRS SMGRNKEKNK EVENEKSPGR SASRSSNISK 51 ASSPTTGTAP RSQSRLSVCP STQDICRICH CEGDEESPLI TPCRCTGTLR 101 FVHQSCLHQW IKSSDTRCCE LCKYDFIMET KLKPLRKWEK LQMTTSERRK 151 IFCSVTFHVI AVTCVVWSLY VLIDRTAEEI KQGNDNGVLE WPFWTKLVVV 201 AIGFTGGLVF MYVQCKVYVQ LWRRLKAYNR VIFVQNCPDT ANKLEKNFPC 251 NVNTEIKDAV VVPVPQTGSN TLPTAEGAPP EVIPV Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 13 - 24 1252.83 1251.82 1251.56 0.26 1 ETASNGRSSMGR 31 - 40 1144.83 1143.82 1143.55 0.27 1 EVENEKSPGR 51 - 77 2920.30 2919.29 2919.39 -0.10 2 ASSPTTGTAPRSQSRLSVCPSTQDICR 124 - 136 1653.96 1652.95 1652.90 0.05 1 YDFIMETKLKPLR 197 - 223 3145.63 3144.62 3144.70 -0.08 1 LVVVAIGFTGGLVFMYVQCKVYVQLWR No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1171.90, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH66008 285 aa linear ROD 30-JUN-2004 DEFINITION RIKEN cDNA 2900024D24 [Mus musculus]. ACCESSION AAH66008 VERSION AAH66008.1 GI:41946803 DBSOURCE accession BC066008.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 285) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse, L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C. M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer, C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang, J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci, P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R. D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J. A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu, X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green, E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers, R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 285) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (02-FEB-2004) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Jim Lin, University of Iowa cDNA Library Preparation: M. Bento Soares, University of Iowa cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: University of Iowa, Dr. M. Bento Soares and Dr. Thomas L. Casavant. Web site: http://genome.uiowa.edu Contact: bento-soares@uiowa.edu; tom-casavant@uiowa.edu Bonaldo,M.F., Akabogu,I., Bair,T., Bair,J., Crouch,K., Davis,A., Fishler,K., Keppel,C., Kucaba,T., Lebeck,M., Melo,A., Schaefer,K., Scheetz,T., Smith,C., Snir,E., Tack,D., Trout, K., Walters,J., Casavant,T., Soares,M.B. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: Plate: Row: Column: 0. Method: conceptual translation. FEATURES Location/ Qualifiers source 1..285 /organism="Mus musculus" / strain="C57BL/6" /db_xref="taxon:10090" /clone="MGC:86120 IMAGE:5703753" /tissue_type="Brain, mouse 15.5 dpc" / clone_lib="NIH_BMAP_EW0" /lab_host="DH10B" /note="Vector: pYX-ASC" Protein 1..285 /product="RIKEN cDNA 2900024D24" Region 75..123 / region_name="The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins" / note="RINGv" /db_xref="CDD:22653" CDS 1..285 /gene="2900024D24Rik" / coded_by="BC066008.1:427..1284" /db_xref="LocusID:72925" / db_xref="MGI:1920175" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦409226 Score: 34 Expect: 32 brain beta spectrin [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 275164; Calculated pI value: 5.66 NCBI BLAST search of gi¦409226 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦2493435 from Mus musculus gi¦30348966 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 13 Sequence Coverage: 8% Matched peptides shown in Bold Red 1 MTTTVATDYD NIEIQQQYSD VNNRWDVDDW DNENSSARLF ERSRIKALAD 51 EREAVQKKTF TKWVNSHLAR VSCRITDLYT DLRDGRMLIK LLEVLSGERL 101 PKPTKGRMRI HCLENVDKAL QFLKEQRVHL ENMGSHDIVD GNHRLTLGLI 151 WTIILRFQIQ DISVETEDNK EKKSAKDALL LWCQMKTAGY PNVNIHNFTT 201 SWRDGMAFNA LIHKHRPDLI DFDKLKKSNA HYNLQNAFNL AEQHLGLTKL 251 LDPEDISVDH PDEKSIITYV VTYYHYFSKM KALAVEGKRI GKVLDNAIET 301 EKMIEKYESL ASDLLEWIEQ TIIILNNRKF ANSLVGVQQQ LQAFNTYRTV 351 EKPPKFTEKG NLEVLLFTIQ SKMRANNQKV YMPREGKLIS DINKAWERLE 401 KAEHERELAL RNELIRQEKL EQLARRFDRK AAMRETWLSE NQRLVSQDNF 451 GFDLPAVEAA TKKHEAIETD IAAYEERVQA VVAVARELEA ENYHDIKRIT 501 ARKDNVIRLW EYLLELLRAR RQRLEMNLGL QKIFQEMLYI MDWMDEMKVL 551 LLSQDYGKHL LGVEDLLQKH ALVEADIAIQ AERVRGVNAS AQKFATDGEG 601 YKPCDPQVIR DRVAHMEFCY QELCQLAAER RARLEESRRL WKFFWEMAEE 651 EGWIREKEKI LSSDDYGKDL TSVMRLLSKH RAFEDEMSGR SGHFEQAIKE 701 GEDMIAEEHF GSEKIRERII YIREQWANLE QLSAIRIKRL EEASLLHQFQ 751 ADADDIDAWM LDILKIVSSN DVGHDEYSTQ SLVKKHKDVA EEITNYRPTI 801 DTLHEQASAL PQAHAESPDV KGRLAGIEER CKEMAELTRL RKQALRDTLA 851 LYKMFSEADA CELWIDEKEQ WLNNMQIPEK LEDLEVIQHR FESLEPEMNN 901 QASRVAVVNQ IARQLMHNGH PSEKEIRAQQ DKLNTRWSQF RELVDRKKDA 951 LLSALSIQNY HLECNETKSW IREKTKVIES TQDLGNDLAG VMALQRKLTG 1001 MERDLVAIEA KLSDLQKEAE KLESEHPDQA QAILSRLAEI SDVWEEMKTT 1051 LKNREASLGE ASKLQQFLRD LDDFQSWLSR TQTAIASEDM PNTLTEAEKL 1101 LTQHENIKNE IDNYEEDYQK MRDMGEMVTQ GQTDAQYMFL RQRLQALDTG 1151 WNELHKMWEN RQNLLSQSHA YQQFLRDTKQ AEAFLNNQEY VLAHTEMPTT 1201 LEGAEAAIKK QEDFMTTMDA NEEKINAVVE TGRRLVSDGN INSDRIQEKV 1251 DSIDDRHRKN REAASELLMR LKDNRDLQKF LQDCQELSLW INEKMLTAQD 1301 MSYDEARNLH SKWLKHQAFM AELASNKEWL DKIEKEGMQL ISEKPETEAV 1351 VKEKLTGLHK MWEVLESTTQ TKAQRLFDAN KAELFTQSCA DLDKWLHGLE 1401 KPGFQSDDYG KDLTQSQYSS EKGNRRRRIR WKFGRKRSRN CRPSPGSSRG 1451 RAQMRVDSKR LTVQTKFMEL LEPLSERKHN LLASKEIHQF NRDVEDEILW 1501 VGERMPLRTS TDHGHNLQTV QLLIKKNQTL QKEIQGHQPR IDDIFERSQN 1551 IITDSSSLNA EAIRQRLADL KQLWGLLIEE TEKRHRRLEE AHKAQQYYFD 1601 AAEAEAWMSE QELYMMSEKR PRMKQSAVSM LKKHQILEQA VEDYAETVHQ 1651 LSKTSRALVA DSHPESERIS MRQSKVDKLY AGLKDLAEER RGKLDERHRL 1701 FQLNREVDDL EQWIAEREVV AGSHELGQDY EHVTMLQERF REFARDTGNI 1751 GQERVDTVNN MADELINSGH SDAATIAEWK DGLNEAWADL LELIDTRTQI 1801 LAASYELHKF YHDAKEIFGR IQDKHKKLPE ELGRDQNTVE TLQRMHTTFE 1851 HDIQALGTQV RQLQEDAARL QAAYAGDKAD DIQKRENEVL EAWKSLLGAC 1901 EGRRVRLVDT GDKFRFFSMV RDLMLWMEDV IRQIEAQEKP RDVSSVELLM 1951 NNHQGIKAEI DARNDSFTAC IELGKSLLAR KHYASEEIKE KLLQLTEKRK 2001 EMIDKWEDRW EWLRLILEVH QFSRDASVAE AWLLGQEPYL SSREIGQSVD 2051 EVEKLIKRHE AFEKSAATWD ERFSALERLT TLELLEVRRQ QEEEERKRRP 2101 PSPDPNTKVS EEAESQQWDT SKGDQVSQNG LPAEQGSPRM AGTMETSEMV 2151 NGAAEQRTSS KESSPVPSPT SDRKAKSALP AQSAATLPAR TLETPAAQME 2201 GFLNRKHEWE AHNKKASSRS WHNVYCVINN QEMGFYKDAK SAASGIPYHS 2251 EVPVSLKEAI CEVALDYKKK KHVFKLRLSD GNEYLFQAKD DEEMNTWIQA 2301 ISSAISSDKH DTSASTQSTP ASSRAQTLPT SVVTITSESS PGKRAEDKEK 2351 DKEKRSTVFG KKK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 59 - 70 1459.93 1458.92 1458.77 0.15 1 TFTKWVNSHLAR 187 - 214 3175.67 3174.67 3174.55 0.12 1 TAGYPNVNIHNFTTSWRDGMAFNALIHK 388 - 401 1714.94 1713.93 1713.94 -0.01 2 LISDINKAWERLEK 435 - 463 3293.91 3292.90 3292.64 0.26 2 ETWLSENQRLVSQDNFGFDLPAVEAATKK 1064 - 1069 804.58 803.57 803.47 0.10 0 LQQFLR 1225 - 1234 1114.82 1113.82 1113.63 0.19 1 INAVVETGRR 1440 - 1451 1330.83 1329.82 1329.63 0.19 1 NCRPSPGSSRGR 1634 - 1653 2338.05 2337.04 2337.16 -0.12 0 HQILEQAVEDYAETVHQLSK 2241 - 2269 3161.64 3160.63 3160.61 0.02 2 SAASGIPYHSEVPVSLKEAICEVALDYKK 2270 - 2275 786.73 785.72 785.49 0.23 2 KKHVFK 2276 - 2289 1653.96 1652.95 1652.85 0.10 1 LRLSDGNEYLFQAK 2325 - 2348 2502.02 2501.02 2501.30 -0.28 2 AQTLPTSVVTITSESSPGKRAEDK 2349 - 2354 776.64 775.63 775.41 0.22 2 EKDKEK No match to: 727.74, 749.74, 864.60, 880.61, 1074.82, 1144.83, 1171.90, 1252.83, 1353.00, 1368.99, 2026.92, 2107.97, 2294.95, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3279.90 LOCUS AAC42040 2363 aa linear ROD 11-FEB-2002 DEFINITION brain beta spectrin [Mus musculus]. ACCESSION AAC42040 VERSION AAC42040.1 GI:409226 DBSOURCE locus MUSSPNA accession M74773.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 2363) AUTHORS Ma,Y., Zimmer,W.E., Riederer,B.M., Bloom,M.L., Barker,J.E., Goodman,S.R. and Goodman SM [corrected to Goodman,S.R.]. TITLE The complete amino acid sequence for brain beta spectrin (beta fodrin): relationship to globin sequences JOURNAL Brain Res. Mol. Brain Res. 18 (1-2), 87-99 (1993) MEDLINE 93240985 PUBMED 8479293 COMMENT Method: conceptual translation. FEATURES Location/Qualifiers source 1..2363 /organism="Mus musculus" /strain="BALB/c" /sub_species="domesticus" / db_xref="taxon:10090" /map="2" Protein 1..2363 /product="brain beta spectrin" Region 55..158 /region_name="Calponin homology domain" / note="CH" /db_xref="CDD:8913" Region 174..277 / region_name="Calponin homology domain" /note="CH" / db_xref="CDD:8913" Region 305..>471 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" / note="SPEC" /db_xref="CDD:5385" Region 423..625 / region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 530..743 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" / db_xref="CDD:5385" Region 745..955 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" / note="SPEC" /db_xref="CDD:5385" Region 851..1061 / region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 958..1169 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" / db_xref="CDD:5385" Region 1171..1380 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 1486..1698 / region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 1593..1805 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" / db_xref="CDD:5385" Region 1806..2013 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 2020..>2075 / region_name="Spectrin repeats" /note="SPEC" /db_xref="CDD:24210" Region 2199..2307 /region_name="Pleckstrin homology domain" / note="PH" /db_xref="CDD:24224" CDS 1..2363 /gene="Spnb-2" / coded_by="M74773.1:367..7458" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦448251 Score: 34 Expect: 32 beta spectrin (beta fodrin) Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 272627; Calculated pI value: 5.63 NCBI BLAST search of gi¦448251 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 13 Sequence Coverage: 8% Matched peptides shown in Bold Red 1 MTTTVATDYD NIEIQQQYSD VNNRWDVDDW DNENSSARLF ERSRIKALAD 51 EREAVQKKTF TKWVNSHLAR VSCRITDLYT DLRDGRMLIK LLEVLSGERL 101 PKPTKGRMRI HCLENVDKAL QFLKEQRVHL ENMGSHDIVD GNHRLTLGLI 151 WTIILRFQIQ DISVETEDNK EKKSAKDALL LWCQMKTAGY PNVNIHNFTT 201 SWRDGMAFNA LIHKHRPDLI DFDKLKKSNA HYNLQNAFNL AEQHLGLTKL 251 LDPEDISVDH PDEKSIITYV VTYYHYFSKM KALAVEGKRI GKVLDNAIET 301 EKMIEKYESL ASDLLEWIEQ TIIILNNRKF ANSLVGVQQQ LQAFNTYRTV 351 EKPPKFTEKG NLEVLLFTIQ SKMRANNQEG KLISDINKAW ERLEKAEHER 401 ELALRNELIR QEKDRKAAMR ETWLSENQRL VSQDNFGFDL PAVEAATKKH 451 EAIETDIAAY EERVQAVVAV ARELEAENYH DIKRITARKD NVIRLWEYLL 501 ELLRARRQRL EMNLGLQKIF QEMLYIMDWM DEMKVLLLSQ DYGKHLLGVE 551 DLLQKHALVE ADIAIQAERV RGVNASAQKF ATDGEGYKPC IRDRVAHMEF 601 CYQELCQLAA ERRARLEESR RLWKFFWEMA EEEGWIREKE KILSSDDYGK 651 DLTSVMRLLS KHRAFEDEMS GRSGHFEQAI KEGEDMIAEE HFGSEKIRER 701 IIYIREQWAN LEQLSAIRIK RLEEASLLHQ FQADADDIDA WMLDILKIVS 751 SNDVGHDEYS TQSLVKKHKD VAEEITNYRP TIDTLHEQAS ALPQAHAESP 801 DVKGRLAGIE ERCHEMAELT RLRKQALRDT LALYKMFSEA DACELWIDEK 851 EQWLNNMQIP EKLEDLEVIQ HRFESLEPEM NNQASRVAVV NQIARQLMHN 901 GHPSEKEIRA QQDKLNTRWS QFRELVDRKK DALLSALSIQ NYHLECNETK 951 SWIREKTKVI ESTQDLGNDL AGVMALQRKL TGMERDLVAI EAKLSDLQKE 1001 AEKLESEHPD QAQILSRLAE ISDVWEEMKT TLKNREASLG EASKLQQFLR 1051 DLDDFQSWLS RTQTAIASED MPNTLTEAEK LLTQHENIKN EIDNYEEDYQ 1101 KMRDMGEMVT QGQTDAQYML RQRLQALDTG WNELHKMWEN RQNLLSQSHA 1151 YQQFLRDTKQ AEAFLNNQEY VLAHTEMPTT LEGAEAAIKK QEDFMTTMDA 1201 NEEKINAVVE TGRRLVSDGN INSDRIQEKV DSIDDRHRKN REAASELLMR 1251 LKDNRDLQKF LQDCQELSLW INEKMLTAQD MSYDEARNLH SKWLKHQAFM 1301 AELASNKEWL DKIEKEGMQL ISEKPETEAV VKEKLTGLHK MWEVLESTTQ 1351 TKAQRLFDAN KAELFTQSCA DLDKWLHGLE KPGFQSDDYG KDLTQSQYSS 1401 EKGNRRRRIR WKFGRKRSRN CRPSPGSSRG RAQMRVDSKR LTVQTKFMEL 1451 LEPLSERKHN LLASKEIHQF NRDVEDEILW VGERMPLRTS TDHGHNLQTV 1501 QLLIKKNQTL QKEIQGHQPR IDDIFERSQN IITDSSSLNA EAIRQRLADL 1551 KQLWGLLIEE TEKRHRRLEE AHKAQQYYFD AAEAEAWMSE QELYMMSEKR 1601 PRMKQSAVSM LKKHQILEQA VEDYAETVHQ LSKTSRALVA DSHPESERIS 1651 MRQSKVDKLY AGLKDLAEER RGKLDERHRL FQLNREVDDL EQWIAEREVV 1701 AGSHELGQDY EHVTMLQERF REFARDTGNI GQERVDTVNN MADELINSGH 1751 SDAAIAEWKD GLNEAWADLL ELIDTRTQIL AASYELHKFY HDAKEIFGRI 1801 QDKHKKLPEE LGRDQNTVET LQRMHTTFEH DIQALGTQVR QLQEDAARLQ 1851 AAYAGDKADD IQKRENEVLE AWKSLLGACE GRRVRLVDTG DKFRFFSMVR 1901 DLMLWMEDVI RQIEAQEKPR DVSSVELLMN NHQGIKAEID ARNDSFTACI 1951 ELGKSLLARK HYASEEIKEK LLQLTEKRKE MIDKWEDRWE WLRLILEVHQ 2001 FSRDASVAEA WLLGQEPYLS SREIGQSVDE VEKLIKRHEA FEKSAATWDE 2051 RFSALERLTT LELLEVRRQQ EEEERKRRPP SPDPNTKVSE EAESQQWDTS 2101 KGDQVSQNGL PAEQGSPRMA GTMETSEMVN GAAEQRTSSK ESSPVPSPTS 2151 DRKAKSALPA QSAATLPART LETPAAQMEG FLNRKHEWEA HNKKASSRSW 2201 HNVYCVINNQ EMGFYKDAKS AASGIPYHSE VPVSLKEAIC EVALDYKKKK 2251 HVFKLRLSDG NEYLFQAKDD EEMNTWIQAI SSAISSDKHD TSASTQSTPA 2301 SSRAQTLPTS VVTITSESSP GKRAEDKEKD KEKRSTVFGK KK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 59 - 70 1459.93 1458.92 1458.77 0.15 1 TFTKWVNSHLAR 187 - 214 3175.67 3174.67 3174.55 0.12 1 TAGYPNVNIHNFTTSWRDGMAFNALIHK 382 - 395 1714.94 1713.93 1713.94 -0.01 2 LISDINKAWERLEK 421 - 449 3293.91 3292.90 3292.64 0.26 2 ETWLSENQRLVSQDNFGFDLPAVEAATKK 1045 - 1050 804.58 803.57 803.47 0.10 0 LQQFLR 1205 - 1214 1114.82 1113.82 1113.63 0.19 1 INAVVETGRR 1420 - 1431 1330.83 1329.82 1329.63 0.19 1 NCRPSPGSSRGR 1614 - 1633 2338.05 2337.04 2337.16 -0.12 0 HQILEQAVEDYAETVHQLSK 2220 - 2248 3161.64 3160.63 3160.61 0.02 2 SAASGIPYHSEVPVSLKEAICEVALDYKK 2249 - 2254 786.73 785.72 785.49 0.23 2 KKHVFK 2255 - 2268 1653.96 1652.95 1652.85 0.10 1 LRLSDGNEYLFQAK 2304 - 2327 2502.02 2501.02 2501.30 -0.28 2 AQTLPTSVVTITSESSPGKRAEDK 2328 - 2333 776.64 775.63 775.41 0.22 2 EKDKEK No match to: 727.74, 749.74, 864.60, 880.61, 1074.82, 1144.83, 1171.90, 1252.83, 1353.00, 1368.99, 2026.92, 2107.97, 2294.95, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3279.90 LOCUS 1916380A 2342 aa linear ROD 19-NOV-1996 DEFINITION beta spectrin (beta fodrin). ACCESSION 1916380A VERSION 1916380A GI:448251 DBSOURCE prf: locus 1916380A; part: brain; state: adult; taxonomy: Mammalia. KEYWORDS Spectrin beta; Fodrin beta; cDNA Clone; Mouse Brain; Antibody Screening; SDS PAGE; Seq Determination; 7520bp; Seq Comparison with Globin; Repeat of 106AAs; Dot Matrix; Spectrin Binding Hemin. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 2342) AUTHORS Ma,Y., Zimmer,W.E., Riederer,B.M. and Goodman,S.R. TITLE The complete amino acid sequence for brain beta spectrin (beta fodrin). Relationship to globin sequences JOURNAL Mol.Brain Res. 18(1/2), 87-99 (1993) COMMENT EC=6.1.1.1. FEATURES Location/ Qualifiers source 1..2342 /organism="Mus musculus" / db_xref="taxon:10090" Region 55..158 /region_name="Calponin homology domain" /note="CH" /db_xref="CDD:8913" Region 174..277 / region_name="Calponin homology domain" /note="CH" / db_xref="CDD:8913" Region 305..>457 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" / note="SPEC" /db_xref="CDD:5385" Region 411..607 / region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 516..725 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" / db_xref="CDD:5385" Region 727..937 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" / note="SPEC" /db_xref="CDD:5385" Region 833..1042 / region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 940..1149 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" / db_xref="CDD:5385" Region 1151..1360 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 1466..1678 / region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 1573..1784 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" / db_xref="CDD:5385" Region 1785..1992 /region_name="Spectrin repeats, found in several proteins involved in cytoskeletal structure" /note="SPEC" /db_xref="CDD:5385" Region 1999..>2054 / region_name="Spectrin repeats" /note="SPEC" /db_xref="CDD:24210" Region 2178..2286 /region_name="Pleckstrin homology domain" / note="PH" /db_xref="CDD:24224" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦19353700 Score: 32 Expect: 45 Unknown (protein for IMAGE:5361724) [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 14756; Calculated pI value: 8.88 NCBI BLAST search of gi¦19353700 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 4 Sequence Coverage: 41% Matched peptides shown in Bold Red 1 EMGFYKDAKS AASGIPYHSE VPVSLKEAIC EVALDYKKKK HVFKLRLSDG 51 NEYLFQAKDD EEMNTWIQAI SSAISSDKHD TSASTQSTPA SSRAQTLPTS 101 VVTITSESSP GKREKDKEKD KEKRFSLFGK KK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 10 - 38 3161.64 3160.63 3160.61 0.02 2 SAASGIPYHSEVPVSLKEAICEVALDYKK 39 - 44 786.73 785.72 785.49 0.23 2 KKHVFK 45 - 58 1653.96 1652.95 1652.85 0.10 1 LRLSDGNEYLFQAK 118 - 123 776.64 775.63 775.41 0.22 2 EKDKEK No match to: 727.74, 749.74, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1459.93, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67, 3279.90, 3293.91 LOCUS AAH24833 132 aa linear ROD 07-AUG-2002 DEFINITION Unknown (protein for IMAGE:5361724) [Mus musculus]. ACCESSION AAH24833 VERSION AAH24833.1 GI:19353700 DBSOURCE accession BC024833.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 132) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (01-MAR-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih. gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: The Cepko Laboratory cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Hale, S.M., Yoon, V.S., Kowis, C.R., Lawrence, S., Martin, R.G., Muzny, D.M., Richards, S., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 54 Row: p Column: 7 This clone was selected for full length sequencing because it passed the following selection criteria: Hexamer frequency ORF analysis. Method: conceptual translation. FEATURES Location/Qualifiers source 1..132 /organism="Mus musculus" / db_xref="taxon:10090" /clone="IMAGE:5361724" /tissue_type="Eye, retina, mouse strain C57Bl¥6" /clone_lib="NIH_MGC_94" / lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..132 / product="Unknown (protein for IMAGE:5361724)" Region <2..76 / region_name="Pleckstrin homology domain" /note="PH" / db_xref="CDD:24224" CDS 1..132 /coded_by="BC024833.1:<1..400" / codon_start=2 Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦38088507 Score: 31 Expect: 55 similar to Spindlin-like protein 2 (SPIN-2) [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 23166; Calculated pI value: 4.97 NCBI BLAST search of gi¦38088507 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 4 Sequence Coverage: 25% Matched peptides shown in Bold Red 1 MEALTGKSSS FTPIVKISHE RKEGDEPITQ WKGTVLDQVP INPSLYLEKY 51 DGIDCMYGLE FHTDKRVLSL KVLSDKVASS RVTDASLADV IIGKAVNHLF 101 EGEHGSKDEW RGMVLGQVSI LDSNFYITYE RDPVLYMYEL LDDYKAGDLL 151 IVEVFSDLPP LDIDLELVDG LIGKHVEYTK DDRSRRVRMV IHQVEAKPTV 201 LHQV Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 22 - 32 1330.83 1329.82 1329.66 0.17 1 KEGDEPITQWK 82 - 111 3293.91 3292.90 3292.65 0.25 2 VTDASLADVIIGKAVNHLFEGEHGSKDEWR 175 - 180 776.64 775.63 775.39 0.24 0 HVEYTK 181 - 186 804.58 803.57 803.40 0.17 2 DDRSRR No match to: 727.74, 749.74, 786.73, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1353.00, 1368.99, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90 LOCUS XP_357994 204 aa linear ROD 01-SEP-2004 DEFINITION similar to Spindlin-like protein 2 (SPIN-2) [Mus musculus]. ACCESSION XP_357994 VERSION XP_357994.1 GI:38088507 DBSOURCE REFSEQ: accession XM_357994.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. COMMENT MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from an annotated genomic sequence (NT_040294) using gene prediction method: GNOMON. Also see: Documentation of NCBI's Annotation Process FEATURES Location/Qualifiers source 1..204 /organism="Mus musculus" /strain="C57BL/6J" / db_xref="taxon:10090" /chromosome="Un" Protein 1..204 / product="similar to Spindlin-like protein 2 (SPIN-2)" Region 17..62 /region_name="Spin/Ssty Family" /note="Spin-Ssty" / db_xref="CDD:9423" Region 17..62 /region_name="Spin/Ssty Family. Spindlin (Spin) is a novel maternal transcript present in the unfertilised egg and early embryo. The Y-linked spermiogenesis - specific transcript (Ssty) is also expressed during gametogenesis and forms part of this Pfam family. Members of this family contain three copies of this 50 residue repeat. The repeat is predicted to contain four beta strands" /note="Spin-Ssty" /db_xref="CDD: pfam02513" Region 92..141 /region_name="Spin/Ssty Family" / note="Spin-Ssty" /db_xref="CDD:9423" Region 92..141 / region_name="Spin/Ssty Family. Spindlin (Spin) is a novel maternal transcript present in the unfertilised egg and early embryo. The Ylinked spermiogenesis -specific transcript (Ssty) is also expressed during gametogenesis and forms part of this Pfam family. Members of this family contain three copies of this 50 residue repeat. The repeat is predicted to contain four beta strands" /note="Spin- Ssty" /db_xref="CDD:pfam02513" CDS 1..204 /gene="LOC385018" / coded_by="XM_357994.1:1..615" /db_xref="GeneID:385018" / db_xref="InterimID:385018" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦37359958 Score: 34 Expect: 32 mKIAA0450 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 73622; Calculated pI value: 9.51 NCBI BLAST search of gi¦37359958 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 16% Matched peptides shown in Bold Red 1 KVKQTLGLKG LFLRGTKPGS LDSHAAGQPL PRPSVSQRLL RRTASAPTKS 51 QKPSRKGFPE LALGTQDAGS EGAADDVAPS SPNPALEAPT QERSGSSSPR 101 GKAPGGEATE ERTLAQVRSP NAPEGPGPAG MAATCMKCVV GSCAGMDVEG 151 LRREQQPSPG PAGSHMAISH QPRARVDSLG GPCCSPSPRA TPGRSKEAPK 201 GPRARRQGPG GGSVSSDSSS PDSPGSPKVA PCQPEGAHRQ QGALQGEMNA 251 LFVQKLEEIR SHSPMFSDTR LFPLQRPISP LCSLEPIAEE PALGPGLPLQ 301 AAAPTGPSQE GSQCPVGLGA KVTSSQQTSL GAFGTLQLRI GGGRENEEPP 351 LRPHNGGISS GPREGTSGRQ TDSKSRSRVP GHLPVVRRAK SEGQVLSELS 401 PTPAVYSDAT GTDRLWQRLE PGSHRDSVSS SSSMSSNDTV IDLSLPSLGL 451 CRSRESIPGV SLGRLTSRPC LASAARPDLP PVTKSKSNPN LRVAGGLPTA 501 PDELQPRPLA PRLTGHHPRP PWHHLTLVGL RDCPVSAKSK SLGDLTADDF 551 APSFQGSTSS LSCGLGSLGV AHQVLEPGIR RDALTEQLRW LTGFQQAGDI 601 TSPTSLGPAG DGSVGGPSFL RRSSSRGQSR VRAIASRARQ AQERQQRLRG 651 QDSRGPPEEE RGTPEGACSV GHEGCVDVPM PAKGAPEQVC GAADSQLLLR 701 L Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 340 - 369 3070.66 3069.65 3069.51 0.15 2 IGGGRENEEPPLRPHNGGISSGPREGTSGR 375 - 387 1459.93 1458.92 1458.85 0.07 2 SRSRVPGHLPVVR 455 - 484 3145.63 3144.62 3144.71 -0.09 1 ESIPGVSLGRLTSRPCLASAARPDLPPVTK 485 - 512 2951.69 2950.69 2950.61 0.08 2 SKSNPNLRVAGGLPTAPDELQPRPLAPR 532 - 538 776.64 775.63 775.35 0.28 0 DCPVSAK 623 - 630 864.60 863.59 863.42 0.17 1 SSSRGQSR No match to: 727.74, 749.74, 786.73, 804.58, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 3131.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS BAC97957 701 aa linear ROD 29-JAN-2004 DEFINITION mKIAA0450 protein [Mus musculus]. ACCESSION BAC97957 VERSION BAC97957.1 GI:37359958 DBSOURCE accession AK129147.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S., Saga,Y., Nagase,T., Ohara,O. and Koga,H. TITLE Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries JOURNAL DNA Res. 10 (4), 167-180 (2003) MEDLINE 22977043 PUBMED 14621295 REFERENCE 2 (residues 1 to 701) AUTHORS Okazaki,N., Kikuno,R., Nagase,T., Ohara, O. and Koga,H. TITLE Direct Submission JOURNAL Submitted (23-JUL- 2003) Hisashi Koga, Kazusa DNA Research Institute, Laboratory for Genome Informatics; 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292- 0818, Japan (E-mail:mouse@kazusa.or.jp, Tel:81-438-52-3919, Fax:81- 438-52-3918) COMMENT The CREATE program supported by Japan science and technology corporation; cDNA full insert sequencing: Kazusa DNA Research Institute; cDNA library construction, clone selection and 5'- & 3'-end one pass sequencing. FEATURES Location/Qualifiers source 1..701 /organism="Mus musculus" /db_xref="taxon:10090" / clone="mbg09026" /tissue_type="brain" /dev_stage="adult" / note="vector:modified pBC SK+" Protein 1..701 /product="mKIAA0450 protein" CDS 1..701 /gene="mKIAA0450" /coded_by="join(AK129147.1: <2662..3462, AK129147.1:3493..4797)" /note="CDS is predicted by in silico analysis. Start codon is not identified." Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦18606452 Score: 33 Expect: 37 Expressed sequence C80913 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 50742; Calculated pI value: 5.03 NCBI BLAST search of gi¦18606452 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦31982769 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 23% Matched peptides shown in Bold Red 1 MVPFGPLAFM PGKLVHTNEV TVLLGDNWFA KCSAKQAVGL VEHRKEHVRK 51 TIDDFKKVLK NFESRVEFTE DLQKMSDAAG DFVDIREEIK SDFEFKGKQR 101 IAHKPHSKPK TSDIFEADFE NGVKPKDTFD ADELWARLEE LERQEELLGE 151 LESKPDTVIA NGEDRVSSEE EKEGADTGVN VVSPVTDSSA ASSCKRRAGN 201 AGLPNGQVNS LNYSVNGSNS YHSNKDDDEE EDDDDDDDDE DDDNESDHAI 251 SADNSIPTIY FSHTVEPKRV RINTGKNTTL KFSEKKEEAK RKRKSGAGSH 301 ATHELPAIKS PADIYRVFVD VVNGEYVPHK SILKSRSREN SVCSDTSESS 351 AADVEDRRGL LRSTSSEEAV ATEAGGSSLD ELQENHPKKP LPSGVSEAFS 401 GTVIEKEFLS PSLAPYSAIA HHALPTIPER KEVPSEVSEE PTKRVSKFRA 451 ARLQQRS Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 14 - 35 2502.02 2501.02 2501.27 -0.26 1 LVHTNEVTVLLGDNWFAKCSAK 101 - 126 2920.30 2919.29 2919.52 -0.23 1 IAHKPHSKPKTSDIFEADFENGVKPK 127 - 143 2107.97 2106.96 2106.99 -0.02 1 DTFDADELWARLEELER 196 - 225 3175.67 3174.67 3174.53 0.14 2 RRAGNAGLPNGQVNSLNYSVNGSNSYHSNK 432 - 443 1330.83 1329.82 1329.63 0.19 0 EVPSEVSEEPTK No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1353.00, 1368.99, 1459.93, 1653.96, 1714.94, 2026.92, 2294.95, 2338.05, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3279.90, 3293.91 LOCUS AAH23029 457 aa linear ROD 29-JUN-2004 DEFINITION Expressed sequence C80913 [Mus musculus]. ACCESSION AAH23029 VERSION AAH23029.1 GI:18606452 DBSOURCE accession BC023029.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 457) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S. I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki, S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G. J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan, K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale, S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman, M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young, A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus, D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 457) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (04-FEB-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Gilbert Smith, Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, A. N., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 53 Row: p Column: 20 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 6755331. Method: conceptual translation. FEATURES Location/Qualifiers source 1..457 / organism="Mus musculus" /strain="FVB/N-3" /db_xref="taxon:10090" / clone="MGC:36045 IMAGE:5374731" /tissue_type="Mammary tumor. MMTVLTR/ INT3 model. 5 month old mouse. Taken by biopsy." / clone_lib="NCI_CGAP_Mam2" /lab_host="DH10B" /note="Vector: pCMVSPORT6" Protein 1..457 /product="expressed sequence C80913" Region <1..71 /region_name="Prefoldin alpha subunit" / note="Prefoldin_alpha" /db_xref="CDD:24168" CDS 1..457 / gene="C80913" /coded_by="BC023029.1:215..1588" / db_xref="LocusID:19777" /db_xref="MGI:1342294" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦1173483 Score: 33 Expect: 38 receptor tyrosine kinase Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 59817; Calculated pI value: 7.95 NCBI BLAST search of gi¦1173483 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦1587975 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 21% Matched peptides shown in Bold Red 1 CPTGFYRVDM NTLRCLKCPQ HSIAESEGST ICTCENGHYR APGEGPQVAC 51 TRPPSAPQNL SFSTSGTQLS LRWEPPRDTG GRHDIRYSVE CLQCRGIAQD 101 GGPCQPCGKG VHFSPAASGL TTSTVQVQGL EPYANYTFTV KPQNRVSGLD 151 SSSPSSASLS INMGHAESLS GLSLKLVKKE PRQLELTWAG SRPRNPGGNL 201 SYELHVLNQD EEWHQMVLEP RVLRTKLQPD TTYIVRVRTL APLGPGPFSP 251 DHEFRTSPPV SRSLTGGEIV AVIFGLLLGI ALLIGIYVFR SRRGQRQRQQ 301 RQRERTTNVG REDKLWLKPY VDLQAYEDPA QGALDFAQEL DPAWLIVDTV 351 IGEGEYGEVY RGALRLPSQD CKTVAIKTLR DTSPDGYWWN FLREATIMGQ 401 FNHPHILRLE GVITKRKPIM IITEFMENGA LDAFLKEREG QLAPGQLVAM 451 LLGIASGMNC LSGHNYVHRD LAARNILVNQ NLCCKVSDLG LTRLLDDFDG 501 TYETQGGKIP IRWTAPGAIA HRIFTTASDV WSFG Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 83 - 109 3161.64 3160.63 3160.41 0.22 2 HDIRYSVECLQCRGIAQDGGPCQPCGK 227 - 255 3279.90 3278.90 3278.72 0.18 2 LQPDTTYIVRVRTLAPLGPGPFSPDHEFR 378 - 393 2026.92 2025.92 2025.97 -0.05 1 TLRDTSPDGYWWNFLR 439 - 469 3293.91 3292.90 3292.63 0.27 0 EGQLAPGQLVAMLLGIASGMNCLSGHNYVHR 523 - 534 1330.83 1329.82 1329.62 0.20 0 IFTTASDVWSFG No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1353.00, 1368.99, 1459.93, 1653.96, 1714.94, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3131.63, 3145.63, 3175.67 LOCUS AAC52384 534 aa linear ROD 01-FEB-1996 DEFINITION receptor tyrosine kinase. ACCESSION AAC52384 VERSION AAC52384.1 GI:1173483 DBSOURCE locus MMU18084 accession U18084.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 534) AUTHORS Lickliter,J.D., Smith,F.M., Olsson,J. E., Mackwell,K.L. and Boyd,A.W. TITLE Embryonic stem cells express multiple Eph-subfamily receptor tyrosine kinases JOURNAL Proc. Natl. Acad. Sci. U.S.A. 93 (1), 145-150 (1996) MEDLINE 96133894 PUBMED 8552593 COMMENT On or before Feb 2, 1996 this sequence version replaced gi:1172084, gi:1172084. Method: conceptual translation. FEATURES Location/Qualifiers source 1..534 / organism="Mus musculus" /strain="129/SV" /db_xref="taxon:10090" / clone="35C15" /cell_line="W9.5" /dev_stage="embryonic stem cells" Protein 1..534 /product="receptor tyrosine kinase" Region 54..145 / region_name="Fibronectin type 3 domain" /note="FN3" / db_xref="CDD:14799" Region 181..256 /region_name="Fibronectin type 3 domain" /note="FN3" /db_xref="CDD:14799" Region 339..>534 / region_name="Tyrosine kinase, catalytic domain" /note="TyrKc" / db_xref="CDD:5392" CDS 1..534 /coded_by="U18084.1:<1..>1602" / codon_start=2 Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦50510365 Score: 31 Expect: 54 mKIAA0113 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 55788; Calculated pI value: 7.23 NCBI BLAST search of gi¦50510365 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 7 Sequence Coverage: 16% Matched peptides shown in Bold Red 1 VTWAEGELLE ESQMEASRLR QKAEELVKDS ELSPPTSAPS LVSFDDLAEL 51 TGQDTKVQVH PATSTAATTT ATATTGNSME KPEPASKSPS NGASSDFEVV 101 PTEEQNSPET GSHPTNMMDL GPPPPEDSNL KLHLQRLETT LSVCAEEPDH 151 SQLFTHLGRM ALEFNRLASK VHKNEQRTSI LQTLCEQLRQ ENEALKAKLD 201 KGLEQRDLAA ERLREENTEL KKLLMNSSCK EGLCGQPSSP KPEGAGKKGV 251 AGQQQASVMA SKVPEAGAFG AAEKKVKLLE QQRMELLEVN KQWDQHFRSM 301 KQQYEQKITE LRQKLVDLQK QVTELEAERE QKQRDFDRKL LLAKSKIEME 351 ETDKEQLTAE AKELRQKVRY LQDQLSPLTR QREYQEKEIQ RLNKALEEAL 401 SIQASPSSPP AAFGSPEGVG GHLRKQELVT QNELLKQQVK IFEEDFQRER 451 SDRERMKEGR KEGRKEGRKE RKKERKKERK KERKKERKK Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 29 - 56 2920.30 2919.29 2919.39 -0.10 0 DSELSPPTSAPSLVSFDDLAELTGQDTK 160 - 166 880.61 879.60 879.43 0.17 0 MALEFNR 278 - 283 786.73 785.72 785.44 0.28 0 LLEQQR 313 - 329 2026.92 2025.92 2026.11 -0.19 2 QKLVDLQKQVTELEAER 321 - 329 1074.82 1073.81 1073.54 0.27 0 QVTELEAER 321 - 332 1459.93 1458.92 1458.73 0.19 1 QVTELEAEREQK 370 - 387 2294.95 2293.95 2294.17 -0.22 2 YLQDQLSPLTRQREYQEK No match to: 727.74, 749.74, 776.64, 804.58, 864.60, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2107.97, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS BAD32168 489 aa linear ROD 28-JUL-2004 DEFINITION mKIAA0113 protein [Mus musculus]. ACCESSION BAD32168 VERSION BAD32168.1 GI:50510365 DBSOURCE accession AK172890.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Okazaki,N., Kikuno,R.F., Ohara,R., Inamoto,S., Koseki,H., Hiraoka, S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K., Kitamura,H., Nagase,T., Ohara,O. and Koga,H. TITLE Prediction of the Coding Sequences of Mouse Homologues of KIAA Gene: IV. The Complete Nucleotide Sequences of 500 Mouse KIAA-Homologous cDNAs Identified by Screening of Terminal Sequences of cDNA Clones Randomly Sampled from Size-Fractionated Libraries JOURNAL DNA Res. 11, 205-218 (2004) REFERENCE 2 (residues 1 to 489) AUTHORS Okazaki, N., Kikuno,R.F., Nagase,T., Ohara,O. and Koga,H. TITLE Direct Submission JOURNAL Submitted (19-MAY-2004) Hisashi Koga, Kazusa DNA Research Institute, Laboratory for Genome Informatics; 2-6-7 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan (E-mail:mouse@kazusa.or. jp, Tel:81-438-52-3919, Fax:81-438-52-3918) COMMENT The CREATE program supported by Japan science and technology corporation; cDNA full insert sequencing: Kazusa DNA Research Institute; cDNA library construction, clone selection and 5'- & 3'-end one pass sequencing. FEATURES Location/Qualifiers source 1..489 /organism="Mus musculus" /db_xref="taxon:10090" /clone="mgj04380" /note="vector: modified pBC SK+" Protein 1..489 /product="mKIAA0113 protein" Region <186..>225 /region_name="Cell shape-determining protein [Cell envelope biogenesis, outer membrane]" /note="MreC" / db_xref="CDD:11502" Region <273..>399 /region_name="Ezrin/radixin/ moesin family" /note="ERM" /db_xref="CDD:24470" CDS 1..489 / gene="mKIAA0113" /coded_by="AK172890.1:<51..>1517" /note="CDS is predicted by in silico analysis. Start codon is not identified." Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦29881548 Score: 31 Expect: 57 Golgi associated PDZ and coiled-coil motif containing [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 50164; Calculated pI value: 5.91 NCBI BLAST search of gi¦29881548 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦16197486 from Mus musculus gi¦31543485 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 21% Matched peptides shown in Bold Red 1 MSAGGPCPAG AGGGPGGSSC PVGVSPGGVS MFRWLEVLEK EFDKAFVDVD 51 LLLGEIDPDQ ADITYEGRQK MTSLSSCFAQ LCHKAQTVSQ INHKLEAQLV 101 DLRSELTETQ AEKVVLEKEV HEQLLQLHST QLQLHAKTGQ SVDSGAIKAK 151 LERELEANKT EKVKEARLEA EVKLLRKENE ALRRHIAVLQ AEVYGARLAA 201 KYLDKELAGR VQQIQLLGRD MKGPAHDKLW NQLEAEIHLH RHKTVIRACR 251 GRNDLKRPMQ APPGHDQDSL KKSQGVGPIR KVLLLKEDHE GLGISITGGK 301 EHGVPILISE IHPGQPADRC GGLHVGDAIL AVNGVNLRDT KHKEAVTILS 351 QQRGEIEFEV VYVAPEVDSD DENVEYEDES GHRYRLYLDE LEGSGNSGAS 401 CKDSSGEMKM LQGYNKKAVR DAHENGDVGA AGESPLDDTA ARAAHLHSLH 451 QKKAY Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 45 - 70 2920.30 2919.29 2919.45 -0.16 1 AFVDVDLLLGEIDPDQADITYEGRQK 85 - 113 3279.90 3278.90 3278.71 0.18 2 AQTVSQINHKLEAQLVDLRSELTETQAEK 151 - 162 1459.93 1458.92 1458.77 0.15 2 LERELEANKTEK 178 - 197 2294.95 2293.95 2294.22 -0.28 2 ENEALRRHIAVLQAEVYGAR 344 - 353 1144.83 1143.82 1143.62 0.20 0 EAVTILSQQR No match to: 727.74, 749.74, 776.64, 786.73, 804.58, 864.60, 880.61, 1074.82, 1114.82, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2026.92, 2107.97, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3293.91 LOCUS AAH51171 455 aa linear ROD 30-JUN-2004 DEFINITION Golgi associated PDZ and coiled-coil motif containing [Mus musculus]. ACCESSION AAH51171 VERSION AAH51171.1 GI:29881548 DBSOURCE accession BC051171.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 455) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J. G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G. D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N. K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T. E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange, C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy, S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne, P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs, R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M. C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 455) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (14-APR-2003) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Susan L. Sullivan, PhD. cDNA Library Preparation: ResGen, Invitrogen Corp cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Institute for Systems Biology http://www.systemsbiology.org contact: amadan@systemsbiology.org Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 105 Row: d Column: 13 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 16716482. Method: conceptual translation. FEATURES Location/Qualifiers source 1..455 / organism="Mus musculus" /db_xref="taxon:10090" /clone="MGC:56850 IMAGE:6307523" /tissue_type="Olfactory epithelium, neonatal mouse, C57Bl/6" /clone_lib="NIH_MGC_129" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..455 /product="golgi associated PDZ and coiled-coil motif containing" Region 279..361 /region_name="PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements" /note="PDZ_signaling" /db_xref="CDD:27595" CDS 1..455 /gene="Gopc" /coded_by="BC051171.1:178..1545" / db_xref="LocusID:94221" /db_xref="MGI:2149946" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦20073181 Score: 31 Expect: 57 Oxa1l protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 46358; Calculated pI value: 9.88 NCBI BLAST search of gi¦20073181 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 19% Matched peptides shown in Bold Red 1 MARNLVCGRW QLLRLLRLQR SYHSVAVSLR PLAAELLAAR RSNGRPPYAL 51 LAVFTPRCIS TSATLFAEAQ VQAPPVVPAT SIPAAVPEVA SGGAADVVQC 101 ATELSFTELG LPWWGAIATC TVLARCLVFP LIVKGQREAA KIHNHMPEMQ 151 KFSARIREAK LAGDQAEFYK ATIEMTRYQK KHDIKLLRPL ILPLTQAPVF 201 ISFFIALREM ANLPVPSLQT GGLWWFQDLT VSDPIYVLPL VVTATMWCVL 251 ELGAETGVQS NDLQFMRNII RVMPLVVLPV TIHFPSAVFM YWLSSNVFSL 301 CQVACLRIPA VRTVLKIPQR VVHDPDKLPP REGFLKSFKK GWKNAEIAQQ 351 LREREQRMQK HLDLAARGPL RQTFTHNPLL QHDPSHPPKA PNSNNSSIKA 401 NAKKPWQDTL G Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 152 - 157 749.74 748.73 748.43 0.30 1 FSARIR 171 - 181 1368.99 1367.98 1367.72 0.26 2 ATIEMTRYQKK 182 - 208 3131.63 3130.62 3130.88 -0.26 1 HDIKLLRPLILPLTQAPVFISFFIALR 337 - 343 880.61 879.60 879.50 0.10 2 SFKKGWK 361 - 389 3293.91 3292.90 3292.73 0.17 2 HLDLAARGPLRQTFTHNPLLQHDPSHPPK No match to: 727.74, 776.64, 786.73, 804.58, 864.60, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3145.63, 3161.64, 3175.67, 3279.90 LOCUS AAH27191 411 aa linear ROD 21-OCT-2003 DEFINITION Oxa1l protein [Mus musculus]. ACCESSION AAH27191 VERSION AAH27191.1 GI:20073181 DBSOURCE accession BC027191.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 411) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse, L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C. M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer, C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang, J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci, P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R. D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J. A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu, X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green, E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers, R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REFERENCE 2 (residues 1 to 411) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (04-APR-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih. gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey E. Green, M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I. M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, A. N., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 37 Row: k Column: 9 This clone was selected for full length sequencing because it passed the following selection criteria: Similarity but not identity to protein. Method: conceptual translation. FEATURES Location/ Qualifiers source 1..411 /organism="Mus musculus" /strain="FVB/N" / db_xref="taxon:10090" /clone="MGC:28641 IMAGE:4223847" / tissue_type="Kidney, normal. 5 month old male mouse." / clone_lib="NCI_CGAP_Kid14" /lab_host="DH10B" /note="Vector: pCMVSPORT6" Protein 1..411 /product="Oxa1l protein" Region 109..320 / region_name="Preprotein translocase subunit YidC [Intracellular trafficking and secretion]" /note="YidC" /db_xref="CDD:COG0706" Region 114..302 /region_name="60Kd inner membrane protein" / note="60KD_IMP" /db_xref="CDD:9384" CDS 1..411 /gene="Oxa1l" / coded_by="BC027191.1:13..1248" /db_xref="LocusID:69089" / db_xref="MGI:1916339" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦26346514 Score: 29 Expect: 86 unnamed protein product [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 48760; Calculated pI value: 9.62 NCBI BLAST search of gi¦26346514 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 18% Matched peptides shown in Bold Red 1 MARNLVCGRW QLLRLLRPQR SYHSVAVSLR PLAAELLAAR RGNGRPPCAL 51 LAVFTPRCIS TSATLFAEAQ VQAPPVIPAT SIPAAVPEVA SGGAADVVQC 101 ATEPSFTELG LGSYTPVGLI QNLLEYIHVD LGLPWWGAIA TCTVLARCLV 151 FPLIVKGQRE AAKIHNHMPE MQKFSARIRE AKLAGDQAEF YKATIEMTRY 201 QKKHDIKLLR PLILPLTQAP VFISFFIALR EMANLPVPSL QTGGLWWFQD 251 LTVSDPIYVL PLVVTATMWC VLELDAETGV QSNDLQFMRN IIRVMPLVVL 301 PVTIHFPSAV FMYWLSSNVF SLCQVACLRI PAVRTVLKIP QRVVHDPDKL 351 PPREGFLKSF KKGWKNAEIA QQLREREQRM QKHLDLAARG PLRQTFTHNP 401 LLQHDPSHPP KAPNSNNSSI KANAKKPWQD TLG Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 174 - 179 749.74 748.73 748.43 0.30 1 FSARIR 193 - 203 1368.99 1367.98 1367.72 0.26 2 ATIEMTRYQKK 204 - 230 3131.63 3130.62 3130.88 -0.26 1 HDIKLLRPLILPLTQAPVFISFFIALR 359 - 365 880.61 879.60 879.50 0.10 2 SFKKGWK 383 - 411 3293.91 3292.90 3292.73 0.17 2 HLDLAARGPLRQTFTHNPLLQHDPSHPPK No match to: 727.74, 776.64, 786.73, 804.58, 864.60, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3145.63, 3161.64, 3175.67, 3279.90 LOCUS BAC36908 433 aa linear ROD 03-APR-2004 DEFINITION unnamed protein product [Mus musculus]. ACCESSION BAC36908 VERSION BAC36908.1 GI:26346514 DBSOURCE accession AK077624.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency fulllength cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) MEDLINE 99279253 PUBMED 10349636 REFERENCE 2 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10 (10), 1617-1630 (2000) MEDLINE 20499374 PUBMED 11042159 REFERENCE 3 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai, T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa, M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka, T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M., Inoue,Y., Kira, A. and Hayashizaki,Y. TITLE RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10 (11), 1757-1771 (2000) MEDLINE 20530913 PUBMED 11076861 REFERENCE 4 AUTHORS The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 5 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 6 (residues 1 to 433) AUTHORS Adachi,J., Aizawa,K., Akimura,T., Arakawa,T., Bono,H., Carninci,P., Fukuda,S., Furuno,M., Hanagaki,T., Hara,A., Hashizume, W., Hayashida,K., Hayatsu,N., Hiramoto,K., Hiraoka,T., Hirozane,T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I., Kasukawa,T., Katoh,H., Kawai,J., Kojima,Y., Kondo,S., Konno,H., Kouda,M., Koya, S., Kurihara,C., Matsuyama,T., Miyazaki,A., Murata,M., Nakamura,M., Nishi,K., Nomura,K., Numazaki,R., Ohno,M., Ohsato,N., Okazaki,Y., Saito,R., Saitoh,H., Sakai,C., Sakai,K., Sakazume,N., Sano,H., Sasaki,D., Shibata,K., Shinagawa,A., Shiraki,T., Sogabe,Y., Tagami, M., Tagawa,A., Takahashi,F., Takaku-Akahira,S., Takeda,Y., Tanaka, T., Tomaru,A., Toya,T., Yasunishi,A., Muramatsu,M. and Hayashizaki, Y. TITLE Direct Submission JOURNAL Submitted (16-APR-2002) Yoshihide Hayashizaki, The Institute of Physical and Chemical Research (RIKEN), Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute; 1-7- 22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (Email: genome-res@gsc.riken.jp, URL:http://genome.gsc.riken.jp/, Tel:81-45-503-9222, Fax:81-45-503-9216) COMMENT cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. Please visit our web site for further details. URL:http://genome. gsc.riken.jp/ URL:http://fantom.gsc.riken.jp/. FEATURES Location/ Qualifiers source 1..433 /organism="Mus musculus" / strain="C57BL/6J" /db_xref="FANTOM_DB:5730481C09" / db_xref="taxon:10090" /clone="5730481C09" /tissue_type="whole body" /clone_lib="RIKEN full-length enriched mouse cDNA library" / dev_stage="8 days embryo" Protein 1..433 /name="unnamed protein product" Region 136..324 /region_name="60Kd inner membrane protein" /note="60KD_IMP" /db_xref="CDD:9384" CDS 1..433 / coded_by="AK077624.1:16..1317" /note="putative similar to CYTOCHROME OXIDASE BIOGENESIS PROTEIN OXA1, MITOCHONDRIAL PRECURSOR (OXA1-LIKE PROTEIN) (OXA1HS) [Homo sapiens] (SWISSPROT|Q15070, evidence: FASTY, 73.8%ID, 88.6%length, match=1311)" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦38372481 Score: 29 Expect: 86 Inner membrane protein OXA1L, mitochondrial precursor (Oxidase assembly 1-like protein) (OXA1-like Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 48702; Calculated pI value: 9.68 NCBI BLAST search of gi¦38372481 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦26342657 from Mus musculus gi¦26336841 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 5 Sequence Coverage: 18% Matched peptides shown in Bold Red 1 MARNLVCGRW QLLRLLRPQR SYHSVAVSLR PLAAELLAAR RGNGRPPCAL 51 LAVFTPRCIS TSATLFAEAQ VQAPPVIPAT SIPAAVPEVA SGGAADVVQC 101 ATEPSFTELG LGSYTPVGLI QNLLEYIHVD LGLPWWGAIA TCTVLARCLV 151 FPLIVKGQRE AAKIHNHMPE MQKFSARIRE AKLAGDQAEF YKATIEMTRY 201 QKKHDIKLLR PLILPLTQAP VFISFFIALR EMANLPVPSL QTGGLWWFQD 251 LTVSDPIYVL PLVVTATMWC VLELGAETGV QSNDLQFMRN IIRVMPLVVL 301 PVTIHFPSAV FMYWLSSNVF SLCQVACLRI PAVRTVLKIP QRVVHDPDKL 351 PPREGFLKSF KKGWKNAEIA QQLREREQRM QKHLDLAARG PLRQTFTHNP 401 LLQHDPSHPP KAPNSNNSSI KANAKKPWQD TLG Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 174 - 179 749.74 748.73 748.43 0.30 1 FSARIR 193 - 203 1368.99 1367.98 1367.72 0.26 2 ATIEMTRYQKK 204 - 230 3131.63 3130.62 3130.88 -0.26 1 HDIKLLRPLILPLTQAPVFISFFIALR 359 - 365 880.61 879.60 879.50 0.10 2 SFKKGWK 383 - 411 3293.91 3292.90 3292.73 0.17 2 HLDLAARGPLRQTFTHNPLLQHDPSHPPK No match to: 727.74, 776.64, 786.73, 804.58, 864.60, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1459.93, 1653.96, 1714.94, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2920.30, 2951.69, 3070.66, 3145.63, 3161.64, 3175.67, 3279.90 LOCUS Q8BGA9 433 aa linear ROD 15-MAR-2004 DEFINITION Inner membrane protein OXA1L, mitochondrial precursor (Oxidase assembly 1- like protein) (OXA1-like protein). ACCESSION Q8BGA9 VERSION Q8BGA9 GI:38372481 DBSOURCE swissprot: locus OXA1_MOUSE, accession Q8BGA9; class: standard. extra accessions:Q8BK01,Q8R091,Q9D8X7,created: Mar 15, 2004. sequence updated: Mar 15, 2004. annotation updated: Mar 15, 2004. xrefs: gi: 12841193, gi: 12841194, gi: 26336840, gi: 26336841, gi: 26342656, gi: 26342657, gi: 26346513, gi: 26346514, gi: 20073180, gi: 20073181, gi: 91655 xrefs (non-sequence databases): MGI1916339, InterProIPR001708, PfamPF02096 KEYWORDS Mitochondrion; Inner membrane; Transit peptide; Transmembrane. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 433) AUTHORS Okazaki,Y., Furuno,M., Kasukawa,T., Adachi,J., Bono,H., Kondo,S., Nikaido,I., Osato,N., Saito,R., Suzuki,H., Yamanaka,I., Kiyosawa,H., Yagi,K., Tomaru,Y., Hasegawa, Y., Nogami,A., Schonbach,C., Gojobori,T., Baldarelli,R., Hill,D.P., Bult,C., Hume,D.A., Quackenbush,J., Schriml,L.M., Kanapin,A., Matsuda,H., Batalov,S., Beisel,K.W., Blake,J.A., Bradt,D., Brusic, V., Chothia,C., Corbani,L.E., Cousins,S., Dalla,E., Dragani,T.A., Fletcher,C.F., Forrest,A., Frazer,K.S., Gaasterland,T., Gariboldi, M., Gissi,C., Godzik,A., Gough,J., Grimmond,S., Gustincich,S., Hirokawa,N., Jackson,I.J., Jarvis,E.D., Kanai,A., Kawaji,H., Kawasawa,Y., Kedzierski,R.M., King,B.L., Konagaya,A., Kurochkin,I. V., Lee,Y., Lenhard,B., Lyons,P.A., Maglott,D.R., Maltais,L., Marchionni,L., McKenzie,L., Miki,H., Nagashima,T., Numata,K., Okido, T., Pavan,W.J., Pertea,G., Pesole,G., Petrovsky,N., Pillai,R., Pontius,J.U., Qi,D., Ramachandran,S., Ravasi,T., Reed,J.C., Reed,D. J., Reid,J., Ring,B.Z., Ringwald,M., Sandelin,A., Schneider,C., Semple,C.A., Setou,M., Shimada,K., Sultana,R., Takenaka,Y., Taylor, M.S., Teasdale,R.D., Tomita,M., Verardo,R., Wagner,L., Wahlestedt, C., Wang,Y., Watanabe,Y., Wells,C., Wilming,L.G., Wynshaw-Boris,A., Yanagisawa,M., Yang,I., Yang,L., Yuan,Z., Zavolan,M., Zhu,Y., Zimmer,A., Carninci,P., Hayatsu,N., Hirozane-Kishikawa,T., Konno, H., Nakamura,M., Sakazume,N., Sato,K., Shiraki,T., Waki,K., Kawai, J., Aizawa,K., Arakawa,T., Fukuda,S., Hara,A., Hashizume,W., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I., Miyazaki,A., Sakai,K., Sasaki,D., Shibata,K., Shinagawa,A., Yasunishi,A., Yoshino,M., Waterston,R., Lander,E.S., Rogers,J., Birney,E. and Hayashizaki,Y. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420 (6915), 563-573 (2002) MEDLINE 22354683 PUBMED 12466851 REMARK SEQUENCE FROM N.A. STRAIN=C57BL/6J; TISSUE=Embryo, Lung, and Pancreas REFERENCE 2 (residues 1 to 433) AUTHORS Strausberg,R.L., Feingold,E. A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner, L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K. H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A. A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki, S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G. J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan, K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale, S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman, M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young, A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S.N., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J.M. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 fulllength human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REMARK SEQUENCE FROM N.A. TISSUE=Kidney COMMENT ------------------------------------------------------------------- This SWISS-PROT entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. The original entry is available from http://www.expasy.ch/sprot and http://www.ebi.ac.uk/sprot ------------------------------------------------------------------. [FUNCTION] Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase (By similarity). [SUBCELLULAR LOCATION] Integral membrane protein. Mitochondria; inner membrane (By similarity). [SIMILARITY] Belongs to the OXA1/oxaA family. FEATURES Location/Qualifiers source 1..433 /organism="Mus musculus" /db_xref="taxon:10090" gene 1..433 /gene="OXA1L" Protein 1..433 /gene="OXA1L" /product="Inner membrane protein OXA1L, mitochondrial precursor" Region (1.432)..433 /gene="OXA1L" / region_name="Mature chain" /note="Inner membrane protein OXA1L." Region 1..(2.433) /gene="OXA1L" /region_name="Transit peptide" / note="Mitochondrion (Potential)." Region 18 /gene="OXA1L" / region_name="Conflict" /note="P -> L (in Ref. 2)." Region 42 / gene="OXA1L" /region_name="Conflict" /note="G -> S (in Ref. 2)." Region 48 /gene="OXA1L" /region_name="Conflict" /note="C -> Y (in Ref. 2)." Region 77 /gene="OXA1L" /region_name="Conflict" /note="I - > V (in Ref. 2)." Region 104 /gene="OXA1L" /region_name="Conflict" / note="P -> L (in Ref. 2)." Region 109..130 /gene="OXA1L" / region_name="Conflict" /note="Missing (in Ref. 2)." Region 109..129 /gene="OXA1L" /region_name="Transmembrane region" / note="Potential." Region 135..155 /gene="OXA1L" / region_name="Transmembrane region" /note="Potential." Region 136..324 /gene="OXA1L" /region_name="60Kd inner membrane protein" / note="60KD_IMP" /db_xref="CDD:9384" Region 208..228 /gene="OXA1L" / region_name="Transmembrane region" /note="Potential." Region 218 / gene="OXA1L" /region_name="Conflict" /note="Q -> H (in Ref. 1; BAB25113)." Region 256..276 /gene="OXA1L" / region_name="Transmembrane region" /note="Potential." Region 275 / gene="OXA1L" /region_name="Conflict" /note="G -> D (in Ref. 1; BAC36908)." Region 294..314 /gene="OXA1L" / region_name="Transmembrane region" /note="Potential." Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦47938931 Score: 31 Expect: 65 C030018L16Rik protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 71590; Calculated pI value: 5.80 NCBI BLAST search of gi¦47938931 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 6 Sequence Coverage: 15% Matched peptides shown in Bold Red 1 MTEGSQIFLL PISTSDSTKE PLSPVASKAQ DPSLLSNRLM IEKQQEEAEW 51 ESINGLLMTH GFKPLCLVKG ADLRDFIVFD KQSSQKMRQI LKTLMEETTR 101 QQSMIRELIE TNQQLKSELQ LEQNRAAHQE QRANDLQQIM DSVKSKIGEL 151 EDESLNRVCQ QQNRIKDLQK EYKMLQMKCQ QYKKNRMEQE GTIASLQKEI 201 HRLAKEEEER ILTQNRVFAH LCRRVPHSVL DKQLLCLIDY YECKLRKLHI 251 QRQFEEDSQS EEKDFTNLGA SPNYKGVLMS LQKQLKESKS RIDVLVGEKL 301 SLQKDLENRP TEHELRLYKQ QVKKLEKTLK KNIKLQDLIG QKKSDDTEKK 351 DEPSKDSHQQ ALIEQSYFQV LCSINSIVHN PRAPVIIYKQ SKGRAPNGNK 401 DIGQDCGFEH LVPIIEMWVD ELTSLKDLYK SLKILSAELV PWHSLKKLDE 451 KEGVKVGDLL FMVDTMLEEV ENQKETSSTP NSQTLQAIVS HFQKLFDVQS 501 LNGVFPRMNE VYTRLGEMNN AVRNLQELLE LDSSSSLCVV VSTVGKLCEI 551 INKDVSEQVK QVLGPEDLQS IIKKLEEHEE FFPAFQAFAN DLLEILEIDD 601 LDAIVPAVKK LKILSY Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 29 - 43 1714.94 1713.93 1713.91 0.02 1 AQDPSLLSNRLMIEK 93 - 116 2920.30 2919.29 2919.48 -0.19 2 TLMEETTRQQSMIRELIETNQQLK 117 - 144 3279.90 3278.90 3278.61 0.29 2 SELQLEQNRAAHQEQRANDLQQIMDSVK 287 - 299 1459.93 1458.92 1458.80 0.12 2 ESKSRIDVLVGEK 554 - 560 804.58 803.57 803.40 0.17 0 DVSEQVK 610 - 616 864.60 863.59 863.55 0.04 2 KLKILSY No match to: 727.74, 749.74, 776.64, 786.73, 880.61, 1074.82, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 2026.92, 2107.97, 2294.95, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3293.91 LOCUS AAH71260 616 aa linear ROD 25-JUN-2004 DEFINITION C030018L16Rik protein [Mus musculus]. ACCESSION AAH71260 VERSION AAH71260.1 GI:47938931 DBSOURCE accession BC071260.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 616) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S. I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki, S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G. J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan, K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale, S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman, M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young, A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus, D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 616) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (01-JUN-2004) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Mark Maconochie, Ph.D. and Nancy L. Freeman, Ph.D. cDNA Library Preparation: ResGen, Invitrogen Corp cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http:// www-shgc.stanford.edu Contact: (Dickson, Mark) mcd@paxil.stanford. edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http:// image.llnl.gov Series: IRAK Plate: 148 Row: l Column: 18 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 24475695. Method: conceptual translation. FEATURES Location/Qualifiers source 1..616 / organism="Mus musculus" /db_xref="taxon:10090" /clone="MGC:78322 IMAGE:6336351" /tissue_type="Embryo, day 9 mouse (C57BL/6 background) otocysts" /clone_lib="NIH_MGC_130" /lab_host="DH10B" / note="Vector: pCMV-SPORT6.1" Protein 1..616 /product="C030018L16Rik protein" CDS 1..616 /gene="C030018L16Rik" / coded_by="BC071260.1:115..1965" /db_xref="LocusID:68121" / db_xref="MGI:1915371" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦14250456 Score: 31 Expect: 66 Tnip1 protein [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 73334; Calculated pI value: 6.01 NCBI BLAST search of gi¦14250456 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 8 Sequence Coverage: 13% Matched peptides shown in Bold Red 1 MEGRGPYRIY DPGGSTPLGE VSAAFERLVE ENTRLKGKMQ GIKMLGELLE 51 ESQMEASRLR QKAEELVKDS ELSPPTSAPS LVSFDDLAEL TGQDTKVQVH 101 PATSTAATTT ATATTGNSME KPEPASKSPS NGASSDFEVV PTEEQNSPET 151 GSHPTNMMDL GPPPPEDSNL KLHLQRLETT LSVCAEEPDH SQLFTHLGRM 201 ALEFNRLASK VHKNEQRTSI LQTLCEQLRQ ENEALKAKLD KGLEQRDLAA 251 ERLREENTEL KKLLMNSSCK EGLCGQPSSP KPEGAGKKGV AGQQQASVMA 301 SKVPEAGAFG AAEKKVKLLE QQRMELLEVN KQWDQHFRSM KQQYEQKITE 351 LRQKLVDLQK QVTELEAERE QKQRDFDRKL LLAKSKIEME ETDKEQLTAE 401 AKELRQKVRY LQDQLSPLTR QREYQEKEIQ RLNKALEEAL SIQASPSSPP 451 AAFGSPEGVG GHLRKQELVT QNELLKQQVK IFEEDFQRER SDRERMNEEK 501 EELKKQVEKL QAQVTLTNAQ LKTLKEEEKA KEALKQQKRK AKASGERYHM 551 EPHPEHVCGA YPYAYPPMPA MVPHHAYKDW SQIRYPPPPV PMEHPPPHPN 601 SRLFHLPEYT WRPPCAGIRN QSSQVMDPPP DRPAEPEPAD LRLPKV Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 69 - 96 2920.30 2919.29 2919.39 -0.10 0 DSELSPPTSAPSLVSFDDLAELTGQDTK 200 - 206 880.61 879.60 879.43 0.17 0 MALEFNR 318 - 323 786.73 785.72 785.44 0.28 0 LLEQQR 353 - 369 2026.92 2025.92 2026.11 -0.19 2 QKLVDLQKQVTELEAER 361 - 369 1074.82 1073.81 1073.54 0.27 0 QVTELEAER 361 - 372 1459.93 1458.92 1458.73 0.19 1 QVTELEAEREQK 410 - 427 2294.95 2293.95 2294.17 -0.22 2 YLQDQLSPLTRQREYQEK 579 - 584 804.58 803.57 803.39 0.18 0 DWSQIR No match to: 727.74, 749.74, 776.64, 864.60, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2107.97, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH08665 646 aa linear ROD 04-OCT-2003 DEFINITION Tnip1 protein [Mus musculus]. ACCESSION AAH08665 VERSION AAH08665.1 GI:14250456 DBSOURCE accession BC008665.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 646) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse, L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C. M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer, C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang, J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci, P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R. D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J. A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu, X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green, E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers, R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REFERENCE 2 (residues 1 to 646) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih. gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey Green M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, A. N., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 16 Row: m Column: 22 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 10946635. Method: conceptual translation. FEATURES Location/Qualifiers source 1..646 / organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" / clone="MGC:11575 IMAGE:3598934" /tissue_type="Mammary tumor. C3(1)- Tag model. Infiltrating ductal carcinoma. 5 month old virgin mouse." /clone_lib="NCI_CGAP_Mam6" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..646 /product="Tnip1 protein" Region 182..439 /region_name="Chromosome segregation ATPases [Cell division and chromosome partitioning]" /note="Smc" /db_xref="CDD: COG1196" Region <226..>265 /region_name="Cell shape-determining protein [Cell envelope biogenesis, outer membrane]" /note="MreC" / db_xref="CDD:11502" Region 309..>441 /region_name="Intermediate filament protein" /note="Filament" /db_xref="CDD:16593" Region <313..>439 /region_name="Ezrin/radixin/moesin family" /note="ERM" / db_xref="CDD:24470" CDS 1..646 /gene="Tnip1" / coded_by="BC008665.1:169..2109" /db_xref="LocusID:57783" / db_xref="MGI:1926194" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦20139295 Score: 30 Expect: 70 Nef-associated factor 1 (Naf1) (A20-binding inhibitor of NF-kappa B activation) (ABIN) (Virion-asso Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 73404; Calculated pI value: 5.77 NCBI BLAST search of gi¦20139295 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi¦4995753 from Mus musculus gi¦10946636 from Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 8 Sequence Coverage: 13% Matched peptides shown in Bold Red 1 MEGRGPYRIY DPGGSTPLGE VSAAFERLVE ENTRLKGKMQ GIKMLGELLE 51 ESQMEASRLR QKAEELVKDS ELSPPTSAPS LVSFDDLAEL TGQDTKVQVH 101 PATSTAATTT ATATTGNSME KPEPASKSPS NGASSDFEVV PTEEQNSPET 151 GSHPTNMMDL GPPPPEDSNL KLHLQRLETT LSVCAEEPDH SQLFTHLGRM 201 ALEFNRLASK VHKNEQRTSI LQTLCEQLRQ ENEALKAKLD KGLEQRDLAA 251 ERLREENTEL KKLLMNSSCK EGLCGQPSSP KPEGAGKKGV AGQQQASVMA 301 SKVPEAGAFG AAEKKVKLLE QQRMELLEVN KQWDQHFRSM KQQYEQKITE 351 LRQKLVDLQK QVTELEAERE QKQRDFDRKL LLAKSKIEME ETDKEQLTAE 401 AKELRQKVRY LQDQLSPLTR QREYQEKEIQ RLNKALEEAL SIQASPSSPP 451 AAFGSPEGVG GHLRKQELVT QNELLKQQVK IFEEDFQRER SDRERMNEEK 501 EELKKQVEKL QAQVTLTNAQ LKTLKEEEKA KEALKQQKRK AKASGERYHM 551 EPHPEHVCGA YPYAYPPMPA MVPHHAYKDW SQIRYPPPPV PMEHPPPHPN 601 SRLFHLPEYT WRPPCAGIRN QSSQVMDPPP DRPAEPESAD NDCDGPQ Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 69 - 96 2920.30 2919.29 2919.39 -0.10 0 DSELSPPTSAPSLVSFDDLAELTGQDTK 200 - 206 880.61 879.60 879.43 0.17 0 MALEFNR 318 - 323 786.73 785.72 785.44 0.28 0 LLEQQR 353 - 369 2026.92 2025.92 2026.11 -0.19 2 QKLVDLQKQVTELEAER 361 - 369 1074.82 1073.81 1073.54 0.27 0 QVTELEAER 361 - 372 1459.93 1458.92 1458.73 0.19 1 QVTELEAEREQK 410 - 427 2294.95 2293.95 2294.17 -0.22 2 YLQDQLSPLTRQREYQEK 579 - 584 804.58 803.57 803.39 0.18 0 DWSQIR No match to: 727.74, 749.74, 776.64, 864.60, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2107.97, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS Q9WUU8 647 aa linear ROD 15-MAR-2004 DEFINITION Nefassociated factor 1 (Naf1) (A20-binding inhibitor of NF-kappa B activation) (ABIN) (Virion-associated nuclear shuttling protein) (VAN) (mVAN). ACCESSION Q9WUU8 VERSION Q9WUU8 GI:20139295 DBSOURCE swissprot: locus NAF1_MOUSE, accession Q9WUU8; class: standard. extra accessions:Q922A9,Q922F7,Q9EPP8,Q9R0X3,created: Feb 28, 2003. sequence updated: Feb 28, 2003. annotation updated: Mar 15, 2004. xrefs: gi: 4995750, gi: 4995751, gi: 4995752, gi: 4995753, gi: 14198252, gi: 14198253, gi: 14250455, gi: 14250456, gi: 9997803, gi: 9997804, gi: 31021776, gi: 31021777 xrefs (non-sequence databases): MGI1926194, GO0005737 KEYWORDS Coiled coil; Nuclear protein; Alternative splicing. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 647) AUTHORS Heyninck,K., De Valck,D., Vanden Berghe,W., Van Criekinge,W., Contreras,R., Fiers,W., Haegeman,G. and Beyaert,R. TITLE The zinc finger protein A20 inhibits TNF-induced NF-kappaBdependent gene expression by interfering with an RIP- or TRAF2- mediated transactivation signal and directly binds to a novel NFkappaB- inhibiting protein ABIN JOURNAL J. Cell Biol. 145 (7), 1471- 1482 (1999) MEDLINE 99315915 PUBMED 10385526 REMARK SEQUENCE FROM N. A. (ISOFORMS 1 AND 2). REFERENCE 2 (residues 1 to 647) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R. D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S. F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R. F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko, L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein, M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S. A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S. W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez, A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez, A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S.N., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J. E., Jones,S.J.M. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899- 16903 (2002) MEDLINE 22388257 PUBMED 12477932 REMARK SEQUENCE FROM N.A. (ISOFORMS 1 AND 3). TISSUE=Breast tumor REFERENCE 3 (residues 1 to 647) AUTHORS Gupta,K., Ott,D., Hope,T.J., Siliciano,R.F. and Boeke,J.D. TITLE A human nuclear shuttling protein that interacts with human immunodeficiency virus type 1 matrix is packaged into virions JOURNAL J. Virol. 74 (24), 11811-11824 (2000) MEDLINE 20541981 PUBMED 11090181 REMARK SEQUENCE OF 97-647 FROM N.A. (ISOFORM 3). COMMENT ------------------------------------------------------------------- This SWISS-PROT entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. The original entry is available from http://www.expasy.ch/sprot and http://www.ebi.ac.uk/sprot ------------------------------------------------------------------. [FUNCTION] Increases cell surface CD4(T4) antigen expression (By similarity). Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal. [SUBUNIT] Interacts with TNFAIP3. [SUBCELLULAR LOCATION] Cytoplasmic. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=ABINl; IsoId=Q9WUU8-1; Sequence=Displayed; Name=2; Synonyms=ABINs; IsoId=Q9WUU8-2; Sequence=VSP_003914; Name=3; IsoId=Q9WUU8-3; Sequence=VSP_003915. [TISSUE SPECIFICITY] Ubiquitous. Abundant in heart and skeletal muscle and expressed at lower levels in thymus, liver, kidney, brain and intestinal tract. FEATURES Location/Qualifiers source 1..647 /organism="Mus musculus" /db_xref="taxon:10090" gene 1..647 / gene="TNIP1" /note="synonym: NAF1" Protein 1..647 /gene="TNIP1" / product="Nef-associated factor 1" Region 1..53 /gene="TNIP1" / region_name="Splicing variant" /note="Missing (in isoform 2). / FTId=VSP_003914." Region 39..72 /gene="TNIP1" / region_name="Domain" /note="Coiled coil (Potential)." Region 95..425 /gene="TNIP1" /region_name="Domain" /note="INTERACTS WITH NEF." Region 97..98 /gene="TNIP1" /region_name="Conflict" /note="VQ -> TR (in Ref. 3)." Region 162..165 /gene="TNIP1" / region_name="Domain" /note="Poly-Pro." Region 209..270 / gene="TNIP1" /region_name="Domain" /note="Coiled coil (Potential)." Region <226..>265 /gene="TNIP1" /region_name="Cell shapedetermining protein [Cell envelope biogenesis, outer membrane]" / note="MreC" /db_xref="CDD:11502" Region 308 /gene="TNIP1" / region_name="Conflict" /note="A -> V (in Ref. 2; AAH08186)." Region 309..>441 /gene="TNIP1" /region_name="Intermediate filament protein" /note="Filament" /db_xref="CDD:16593" Region 311..551 / gene="TNIP1" /region_name="Domain" /note="Coiled coil (Potential)." Region <313..>439 /gene="TNIP1" /region_name="Ezrin/radixin/moesin family" /note="ERM" /db_xref="CDD:24470" Region 533 /gene="TNIP1" / region_name="Conflict" /note="A -> V (in Ref. 2; AAH08186)." Region 537..543 /gene="TNIP1" /region_name="Domain" /note="Nuclear localization signal (Potential)." Region 552..647 /gene="TNIP1" / region_name="Domain" /note="Pro-rich." Region 638..647 / gene="TNIP1" /region_name="Splicing variant" /note="SADNDCDGPQ -> PADLRLPKV (in isoform 3). /FTId=VSP_003915." Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦14198253 Score: 30 Expect: 70 TNFAIP3 interacting protein 1 [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 73460; Calculated pI value: 5.77 NCBI BLAST search of gi¦14198253 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 8 Sequence Coverage: 13% Matched peptides shown in Bold Red 1 MEGRGPYRIY DPGGSTPLGE VSAAFERLVE ENTRLKGKMQ GIKMLGELLE 51 ESQMEASRLR QKAEELVKDS ELSPPTSAPS LVSFDDLAEL TGQDTKVQVH 101 PATSTAATTT ATATTGNSME KPEPASKSPS NGASSDFEVV PTEEQNSPET 151 GSHPTNMMDL GPPPPEDSNL KLHLQRLETT LSVCAEEPDH SQLFTHLGRM 201 ALEFNRLASK VHKNEQRTSI LQTLCEQLRQ ENEALKAKLD KGLEQRDLAA 251 ERLREENTEL KKLLMNSSCK EGLCGQPSSP KPEGAGKKGV AGQQQASVMA 301 SKVPEAGVFG AAEKKVKLLE QQRMELLEVN KQWDQHFRSM KQQYEQKITE 351 LRQKLVDLQK QVTELEAERE QKQRDFDRKL LLAKSKIEME ETDKEQLTAE 401 AKELRQKVRY LQDQLSPLTR QREYQEKEIQ RLNKALEEAL SIQASPSSPP 451 AAFGSPEGVG GHLRKQELVT QNELLKQQVK IFEEDFQRER SDRERMNEEK 501 EELKKQVEKL QAQVTLTNAQ LKTLKEEEKA KEVLKQQKRK AKASGERYHM 551 EPHPEHVCGA YPYAYPPMPA MVPHHAYKDW SQIRYPPPPV PMEHPPPHPN 601 SRLFHLPEYT WRPPCAGIRN QSSQVMDPPP DRPAEPESAD NDCDGPQ Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 69 - 96 2920.30 2919.29 2919.39 -0.10 0 DSELSPPTSAPSLVSFDDLAELTGQDTK 200 - 206 880.61 879.60 879.43 0.17 0 MALEFNR 318 - 323 786.73 785.72 785.44 0.28 0 LLEQQR 353 - 369 2026.92 2025.92 2026.11 -0.19 2 QKLVDLQKQVTELEAER 361 - 369 1074.82 1073.81 1073.54 0.27 0 QVTELEAER 361 - 372 1459.93 1458.92 1458.73 0.19 1 QVTELEAEREQK 410 - 427 2294.95 2293.95 2294.17 -0.22 2 YLQDQLSPLTRQREYQEK 579 - 584 804.58 803.57 803.39 0.18 0 DWSQIR No match to: 727.74, 749.74, 776.64, 864.60, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2107.97, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS AAH08186 647 aa linear ROD 29-JUN-2004 DEFINITION TNFAIP3 interacting protein 1 [Mus musculus]. ACCESSION AAH08186 VERSION AAH08186.1 GI:14198253 DBSOURCE accession BC008186.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 647) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S. I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki, S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G. J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan, K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale, S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman, M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young, A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus, D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S. A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 647) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (22-MAY-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892- 2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Gilbert Smith, Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, A. N., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 10 Row: p Column: 20 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 10946635. Method: conceptual translation. FEATURES Location/Qualifiers source 1..647 / organism="Mus musculus" /strain="CZECH II" /db_xref="taxon:10090" / clone="MGC:5698 IMAGE:3589311" /tissue_type="Mammary tumor metastatized to lung. Tumor arose spontaneously from a senescent normal mammary (clonal) outgrowth infected with the virus MMTV." / clone_lib="NCI_CGAP_Lu29" /lab_host="DH10B" /note="Vector: pCMVSPORT6" Protein 1..647 /product="TNFAIP3 interacting protein 1" Region <226..>265 /region_name="Cell shape-determining protein [Cell envelope biogenesis, outer membrane]" /note="MreC" / db_xref="CDD:11502" Region 309..>441 /region_name="Intermediate filament protein" /note="Filament" /db_xref="CDD:16593" Region <313..>439 /region_name="Ezrin/radixin/moesin family" /note="ERM" / db_xref="CDD:24470" CDS 1..647 /gene="Tnip1" / coded_by="BC008186.1:62..2005" /db_xref="LocusID:57783" / db_xref="MGI:1926194" Mascot: http://www.matrixscience.com/ Mascot Search Results Protein View Match to: gi¦4995751 Score: 29 Expect: 98 ABINs, A20-binding inhibitor of NF-kappa B activation (small) [Mus musculus] Found in search of F:¥LS2N-S005¥大島 朋子先生¥PMF¥040921LS2NS005¥data ¥PMFtrim¥trim+LS2N-S005 B1_0001.mgf Nominal mass (Mr): 67556; Calculated pI value: 5.87 NCBI BLAST search of gi¦4995751 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Fixed modifications: Carbamidomethyl (C) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 32 Number of mass values matched: 8 Sequence Coverage: 14% Matched peptides shown in Bold Red 1 MEASRLRQKA EELVKDSELS PPTSAPSLVS FDDLAELTGQ DTKVQVHPAT 51 STAATTTATA TTGNSMEKPE PASKSPSNGA SSDFEVVPTE EQNSPETGSH 101 PTNMMDLGPP PPEDSNLKLH LQRLETTLSV CAEEPDHSQL FTHLGRMALE 151 FNRLASKVHK NEQRTSILQT LCEQLRQENE ALKAKLDKGL EQRDLAAERL 201 REENTELKKL LMNSSCKEGL CGQPSSPKPE GAGKKGVAGQ QQASVMASKV 251 PEAGAFGAAE KKVKLLEQQR MELLEVNKQW DQHFRSMKQQ YEQKITELRQ 301 KLVDLQKQVT ELEAEREQKQ RDFDRKLLLA KSKIEMEETD KEQLTAEAKE 351 LRQKVRYLQD QLSPLTRQRE YQEKEIQRLN KALEEALSIQ ASPSSPPAAF 401 GSPEGVGGHL RKQELVTQNE LLKQQVKIFE EDFQRERSDR ERMNEEKEEL 451 KKQVEKLQAQ VTLTNAQLKT LKEEEKAKEA LKQQKRKAKA SGERYHMEPH 501 PEHVCGAYPY AYPPMPAMVP HHAYKDWSQI RYPPPPVPME HPPPHPNSRL 551 FHLPEYTWRP PCAGIRNQSS QVMDPPPDRP AEPESADNDC DGPQ Residue Number Increasing Mass Decreasing Mass Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 16 - 43 2920.30 2919.29 2919.39 -0.10 0 DSELSPPTSAPSLVSFDDLAELTGQDTK 147 - 153 880.61 879.60 879.43 0.17 0 MALEFNR 265 - 270 786.73 785.72 785.44 0.28 0 LLEQQR 300 - 316 2026.92 2025.92 2026.11 -0.19 2 QKLVDLQKQVTELEAER 308 - 316 1074.82 1073.81 1073.54 0.27 0 QVTELEAER 308 - 319 1459.93 1458.92 1458.73 0.19 1 QVTELEAEREQK 357 - 374 2294.95 2293.95 2294.17 -0.22 2 YLQDQLSPLTRQREYQEK 526 - 531 804.58 803.57 803.39 0.18 0 DWSQIR No match to: 727.74, 749.74, 776.64, 864.60, 1114.82, 1144.83, 1171.90, 1252.83, 1330.83, 1353.00, 1368.99, 1653.96, 1714.94, 2107.97, 2338.05, 2502.02, 2951.69, 3070.66, 3131.63, 3145.63, 3161.64, 3175.67, 3279.90, 3293.91 LOCUS CAB44239 594 aa linear ROD 20-JUL-1999 DEFINITION ABINs, A20- binding inhibitor of NF-kappa B activation (small) [Mus musculus]. ACCESSION CAB44239 VERSION CAB44239.1 GI:4995751 DBSOURCE embl locus MMU242777, accession AJ242777.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS Heyninck,K., De Valck,D., Vanden Berghe,W., Van Criekinge,W., Contreras,R., Fiers,W., Haegeman,G. and Beyaert,R. TITLE The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaBinhibiting protein ABIN JOURNAL J. Cell Biol. 145 (7), 1471-1482 (1999) MEDLINE 99315915 PUBMED 10385526 REFERENCE 2 (residues 1 to 594) AUTHORS Heyninck,K.M.S. TITLE Direct Submission JOURNAL Submitted (28-MAY-1999) Heyninck K.M.S., Department of Molecular Biology, University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, BELGIUM FEATURES Location/Qualifiers source 1..594 /organism="Mus musculus" /db_xref="taxon:10090" Protein 1..594 /product="ABINs, A20-binding inhibitor of NF-kappa B activation (small)" Region <173..>212 /region_name="Cell shape-determining protein [Cell envelope biogenesis, outer membrane]" /note="MreC" / db_xref="CDD:11502" Region 256..>388 /region_name="Intermediate filament protein" /note="Filament" /db_xref="CDD:16593" Region <260..>386 /region_name="Ezrin/radixin/moesin family" /note="ERM" / db_xref="CDD:24470" CDS 1..594 /gene="ABINs" / coded_by="AJ242777.1:104..1888" /db_xref="GOA:Q9WUU8" / db_xref="UniProt/Swiss-Prot:Q9WUU8" Mascot: http://www.matrixscience.com/ Home Welcome This site features Mascot, a powerful search engine that uses mass spectrometry data to identify proteins from primary sequence databases. To assist you, the help text for Mascot forms a substantial knowledge base concerning protein identification by MS. If this is your first visit, please check for browser compatibility and read the small print. If you include results from Mascot in a publication, please cite either this URL or Electrophoresis, 20(18) 3551-67 (1999) (abstract). We value your feedback and suggestions for new features. If you find any problems, errors, oversights, or just get unexpected results then please let us know. For information on licensing Mascot for in-house use, please refer to our Products and Support pages. For recent news, check What's New. Matrix Science develops and markets software products which integrate mass spectrometry into bioinformatics. Our interests extend to all aspects of mass spectrometry in the life sciences. Please contact us to discuss: ● Developing new applications ● Consultancy in mass spectrometry and bioinformatics ● Systems analysis and integration Collaborations Mascot incorporates code from Mowse, developed by Darryl Pappin and David Perkins when working at the former Imperial Cancer Research Fund, and licensed from its technology transfer subsidiary, Cancer Research Technology. Matrix Science is collaborating with BioVisioN to develop improved data reduction software. LabVantage Solutions and Matrix Science are working together to develop data management and data mining solutions for proteomics. We are grateful to the Swiss Institute of Bioinformatics for permission to make Swiss-Prot available on this web site for searching with Mascot. Copyright © 2003 Matrix Science Ltd. All Rights Reserved. Last Updated undefined