Raw file Experiment Fraction Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV285 WT_Rep1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 5957 34239 0 38203 30275 0 7928 6262 6093 0 169 16.39 20.13 NaN 2.13 5579 824119 32043 3.89 1052 3.28 125404 28218 26955 22.5 31.83 1559 5.52 + 0.4079 0.60812 0.27585 0.42135 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV286 WT_Rep1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6215 45910 0 50052 41768 0 8284 14952 14540 0 412 29.87 34.81 NaN 4.97 12599 958316 43539 4.54 1195 2.74 169732 39464 38832 23.25 26.33 1750 4.43 + 0.39638 0.58844 0.27024 0.42189 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV287 WT_Rep1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6087 46121 0 49795 42447 0 7348 16719 16349 0 370 33.58 38.52 NaN 5.04 12969 978421 43848 4.48 1137 2.59 172325 40201 39711 23.33 25.87 1724 4.29 + 0.37806 0.55919 0.24283 0.37758 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV288 WT_Rep2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 5750 35448 0 39374 31522 0 7852 6927 6726 0 201 17.59 21.34 NaN 2.56 6230 828663 33172 4 1168 3.52 129740 29305 28089 22.59 30.93 1672 5.71 + 0.41539 0.62086 0.28217 0.43122 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV289 WT_Rep2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6795 50654 0 55501 45807 0 9694 16502 16018 0 484 29.73 34.97 NaN 4.99 13703 1039786 48108 4.63 1290 2.68 182257 43310 42499 23.76 26.99 1938 4.47 + 0.40409 0.59761 0.28033 0.43401 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV290 WT_Rep2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6227 41813 0 45227 38399 0 6828 14257 13964 0 293 31.52 36.37 NaN 4.29 11347 943166 39812 4.22 1069 2.69 163105 36421 35908 22.33 25.14 1543 4.24 + 0.37956 0.56249 0.24128 0.37489 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV291 WT_Rep3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6878 30918 0 35249 26587 0 8662 4073 3987 0 86 11.55 15 NaN 0.99 3700 842584 28435 3.37 1210 4.26 109770 24275 23126 22.11 32.64 1732 7.13 + 0.39735 0.60165 0.26079 0.40231 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV292 WT_Rep3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6273 45878 0 50872 40884 0 9988 13809 13359 0 450 27.14 32.68 NaN 4.51 11694 937140 43081 4.6 1360 3.16 165090 38336 37558 23.22 26.74 1928 5.03 + 0.40887 0.60681 0.27993 0.43575 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV293 WT_Rep3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6184 37281 0 40600 33962 0 6638 11262 11040 0 222 27.74 32.51 NaN 3.34 9198 867826 35093 4.04 1136 3.24 143007 31804 31346 22.24 25.37 1575 4.95 + 0.38017 0.5631 0.23033 0.35283 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV294 GPX_Rep1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 8046 28751 0 34012 23490 0 10522 2076 2019 0 57 6.1 8.6 NaN 0.54 1917 900135 25793 2.87 1386 5.37 100370 20855 19711 20.78 32.42 2011 9.64 + 0.37667 0.5561 0.24128 0.36417 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV295 GPX_Rep1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6130 42407 0 47133 37681 0 9452 9841 9467 0 374 20.88 25.12 NaN 3.96 8601 878950 39334 4.48 1598 4.06 149044 34686 33877 23.27 27.4 2192 6.32 + 0.41304 0.60798 0.27634 0.42536 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV296 GPX_Rep1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6509 32143 0 35891 28395 0 7496 5417 5283 0 134 15.09 18.61 NaN 1.79 4689 778528 29617 3.8 1325 4.47 110676 25910 25334 23.41 28.5 1771 6.84 + 0.37237 0.54676 0.21735 0.32933 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV297 GPX_Rep2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 7229 30578 0 35684 25472 0 10212 1545 1483 0 62 4.33 5.82 NaN 0.61 1427 818446 27442 3.35 1506 5.49 99406 22571 21310 22.71 34.49 2199 9.74 + 0.39975 0.56953 0.26092 0.38746 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV298 GPX_Rep2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6451 43294 0 48881 37707 0 11174 7213 6910 0 303 14.76 18.33 NaN 2.71 6325 836640 39674 4.74 1801 4.54 140170 34225 33393 24.42 28.52 2480 7.25 + 0.42471 0.61448 0.28487 0.43551 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV299 GPX_Rep2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 5970 35416 0 39327 31505 0 7822 6138 6014 0 124 15.61 19.09 NaN 1.59 5145 805024 32634 4.05 1427 4.37 125667 28753 28299 22.88 26.23 1939 6.74 + 0.39999 0.56782 0.23538 0.35375 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV300 GPX_Rep3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 7402 30581 0 35759 25403 0 10356 2072 2019 0 53 5.79 7.95 NaN 0.51 1885 883667 27594 3.12 1411 5.11 107842 22634 21374 20.99 33.65 2099 9.27 + 0.4383 0.62463 0.28938 0.41778 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV301 GPX_Rep3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6308 43261 0 48421 38101 0 10320 7817 7505 0 312 16.14 19.7 NaN 3.02 6755 868232 40011 4.61 1648 4.12 145604 34902 34071 23.97 28.04 2333 6.68 + 0.42162 0.61151 0.28057 0.42802 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV302 GPX_Rep3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Acetyl (K) Carbamidomethyl (C) False False 1 3 Standard 6166 35391 0 39309 31473 0 7836 6719 6593 0 126 17.09 20.95 NaN 1.61 5628 837272 32702 3.91 1357 4.15 129858 28861 28368 22.23 25.95 1821 6.31 + 0.39581 0.57572 0.23696 0.356 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV324 WT_Rep1_input 32767 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6222 42149 0 45473 38825 0 6648 17245 16893 0 352 37.92 43.51 NaN 5.29 13942 869217 40165 4.62 1172 2.92 148534 36821 36061 24.79 28.73 1675 4.55 + 0.36395 0.54403 0.23468 0.36175 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV325 WT_Rep2_input 32767 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6565 49961 0 53692 46230 0 7462 22241 21786 0 455 41.42 47.13 NaN 6.1 18238 1017897 47864 4.7 1261 2.63 174200 44040 43003 25.28 28.97 1869 4.24 + 0.36732 0.55533 0.24524 0.37997 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV326 WT_Rep3_input 32767 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6355 48221 0 51667 44775 0 6892 22145 21659 0 486 42.86 48.37 NaN 7.05 18046 988746 46294 4.68 1178 2.54 172569 42748 41833 24.77 28.24 1718 4.02 + 0.36758 0.558 0.24196 0.37939 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV327 GPX_Rep1_input 32767 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6132 47929 0 51154 44704 0 6450 22185 21798 0 387 43.37 48.76 NaN 6 17781 996554 46058 4.62 1168 2.54 172691 42672 41772 24.71 28.1 1719 4.03 + 0.36408 0.5525 0.24859 0.3908 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV328 GPX_Rep2_input 32767 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6174 48719 0 52176 45262 0 6914 20668 20287 0 381 39.61 44.82 NaN 5.51 16844 1039856 46886 4.51 1136 2.42 178684 43286 42260 24.22 27.51 1676 3.87 + 0.3702 0.55755 0.25122 0.39088 180831_C18_017_QHF1_PTM_Rev02_#18_031_PTV329 GPX_Rep3_input 32767 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6230 47434 0 51057 43811 0 7246 20313 19916 0 397 39.78 45.46 NaN 5.48 16332 1013795 45456 4.48 1217 2.68 173938 41749 40696 24 27.65 1757 4.21 + 0.37166 0.56395 0.2474 0.38534 Total 154255 974497 0 1074509 874485 0 200024 278398 271708 0 6690 25.91 31.07 NaN 3.34 48613 21752980 3489683 816047 795386 23.38 28.03 44680 5.48 0.3844 0.57323 0.2537 0.39344