<?xml version="1.0" standalone="yes"?>
<FractionTreatmentDataSet xmlns="BDAL/HyStar/FractionTreatmentDataSet.xsd">
  <Method Author="Narumon" Comment="" DataAcqRunTime="40" StartDelay="0" HyStarVersion="3.2.44.0" ComputerName="HPZ440" xmlns="" />
  <TimeTable Function="FlowRate" Time="0" DeviceType="Pump" DeviceNumber="1" xmlns="">
    <TableValues Value="0.0003" />
  </TimeTable>
  <TimeTable Function="SolventMix" Time="0" DeviceType="Pump" DeviceNumber="1" xmlns="">
    <TableValues Value="100" />
    <TableValues Value="0" />
    <TableValues Value="0" />
    <TableValues Value="0" />
  </TimeTable>
  <Signal SlopeThreshold="0" ID="0" FullName="214.000000" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="1" FullName="254.000000" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="2" FullName="BPC,All" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="MassSpec" DeviceNumber="1" xmlns="" />
  <Signal SlopeThreshold="0" ID="3" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="4" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="5" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="6" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="7" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Signal SlopeThreshold="0" ID="8" SmoothingWidth="2" Slope="20" Sensitivity="5" DeviceType="None" DeviceNumber="0" xmlns="" />
  <Detector SliceWidth="400" AutoZero="0" InvertSignal="0" Offset="0" DataChannel="1" DoReference="false" ReferenceStart="254" ReferenceWidth="10" SpecStart="0" SpecEnd="0" DoSaveSpectra="true" SaveInterval="1" SaveType="1" DeviceNumber="1" xmlns="" />
  <Pump MinPressure="0" MaxPressure="500" Start="false" Runtime="40" DeviceNumber="1" xmlns="" />
  <Solvent ID="0" DeviceType="Pump" DeviceNumber="1" xmlns="" />
  <Solvent ID="1" DeviceType="Pump" DeviceNumber="1" xmlns="" />
  <Solvent ID="2" DeviceType="Pump" DeviceNumber="1" xmlns="" />
  <Solvent ID="3" DeviceType="Pump" DeviceNumber="1" xmlns="" />
  <ExtendedParameters DeviceFullName="Dionex System	SYSTEM1	SYSTEM	" Parameter="&lt;?xml version=&quot;1.0&quot; encoding=&quot;windows-1252&quot;?&gt; &#xD;&#xA;&#xD;&#xA;&lt;root&gt;&#xD;&#xA;    &lt;HyStarInfo ModuleName=&quot;Dionex System&quot; StyleSheet=&quot;NoStyleSheet.xls&quot; MethodName = &quot;PGM::\HCTULTRAPTM_local\NanoLC_HCT\TempPgm&quot;&gt;&#xD;&#xA;&#xD;&#xA;	&lt;MethodData TotalRunTime=&quot;40.000000&quot; AcqDelay=&quot;0.0&quot; AcqTime=&quot;0.000000&quot; Flow=&quot;0.000300&quot;&gt;&#xD;&#xA;	     &#xD;&#xA;        &lt;DetectorInfo DetectorType=&quot;UVD&quot; Slicewidth=&quot;400.000000&quot; WaveStart=&quot;0.0&quot; WaveEnd=&quot;0.0&quot;/&gt;&#xD;&#xA;	&lt;/MethodData&gt;&#xD;&#xA;&#xD;&#xA;    &lt;/HyStarInfo&gt;&#xD;&#xA;&#xD;&#xA;    &lt;ModuleInfo Value=&quot;&quot;&gt; &#xD;&#xA;			AcquireExclusiveAccess&#xD;&#xA;	;Initialize all Hystar synchronisation properties to 0.&#xD;&#xA;	ReadyToRun = 	0&#xD;&#xA;	DoInject = 	0&#xD;&#xA;	InjectResponse = 	0&#xD;&#xA;	ColumnOven.TempCtrl = 	On&#xD;&#xA;	ColumnOven.Temperature.Nominal = 	60.0 [°C]&#xD;&#xA;	ColumnOven.Temperature.LowerLimit = 	20.0 [°C]&#xD;&#xA;	ColumnOven.Temperature.UpperLimit = 	82.6 [°C]&#xD;&#xA;	ColumnOven.ReadyTempDelta = 	0.5 [°C]&#xD;&#xA;	LoadingPump.Pressure.LowerLimit = 	0 [bar]&#xD;&#xA;	LoadingPump.Pressure.UpperLimit = 	350 [bar]&#xD;&#xA;	LoadingPump.MaximumFlowRampDown = 	20 [µl/min²]&#xD;&#xA;	LoadingPump.MaximumFlowRampUp = 	20 [µl/min²]&#xD;&#xA;	LoadingPump.%A.Equate = 	&quot;0.1% HFBA in Water&quot;&#xD;&#xA;	LoadingPump.%B.Equate = 	&quot;%B&quot;&#xD;&#xA;	LoadingPump.%C.Equate = 	&quot;%C&quot;&#xD;&#xA;	MicroPump.Pressure.LowerLimit = 	0 [bar]&#xD;&#xA;	MicroPump.Pressure.UpperLimit = 	350 [bar]&#xD;&#xA;	MasterPressure.LowerLimit = 	0 [bar]&#xD;&#xA;	MasterPressure.UpperLimit = 	400 [bar]&#xD;&#xA;	MicroPump.MaximumFlowRampDown = 	1.001 [µl/min²]&#xD;&#xA;	MicroPump.MaximumFlowRampUp = 	0.400 [µl/min²]&#xD;&#xA;	MicroPump.%A.Equate = 	&quot;0.1% FA in Water&quot;&#xD;&#xA;	MicroPump.%B.Equate = 	&quot;80% ACN in Water+0.1% FA&quot;&#xD;&#xA;	MicroPump.%C.Equate = 	&quot;%C&quot;&#xD;&#xA;	DrawSpeed = 	200 [nl/s]&#xD;&#xA;	DrawDelay = 	5000 [ms]&#xD;&#xA;	DispSpeed = 	2000 [nl/s]&#xD;&#xA;	DispenseDelay = 	2000 [ms]&#xD;&#xA;	WasteSpeed = 	4000 [nl/s]&#xD;&#xA;	WashSpeed = 	4000 [nl/s]&#xD;&#xA;	SampleHeight = 	2.000 [mm]&#xD;&#xA;	PunctureDepth = 	10.000 [mm]&#xD;&#xA;	WashVolume = 	100.000 [µl]&#xD;&#xA;	RinseBetweenReinjections = 	Yes&#xD;&#xA;	LowDispersionMode = 	Off&#xD;&#xA;	InjectMode = 	ulPickUp&#xD;&#xA;	FlushVolume = 	5.000 [µl]&#xD;&#xA;	LoadingPump_Pressure.Step = 	Auto&#xD;&#xA;	LoadingPump_Pressure.Average = 	Off&#xD;&#xA;	MicroPump_MasterPressure.Step = 	Auto&#xD;&#xA;	MicroPump_MasterPressure.Average = 	Off&#xD;&#xA;	ColumnOven_Temp.Step = 	Auto&#xD;&#xA;	ColumnOven_Temp.Average = 	On&#xD;&#xA;	ColumnPressure.Step = 	Auto&#xD;&#xA;	ColumnPressure.Average = 	Off&#xD;&#xA;	ColumnOven_FC_BridgeFlow.Step = 	Auto&#xD;&#xA;	ColumnOven_FC_BridgeFlow.Average = 	On&#xD;&#xA;	ColumnOven_FC_Stepper.Step = 	Auto&#xD;&#xA;	ColumnOven_FC_Stepper.Average = 	On&#xD;&#xA;	FirstTransportVial = 	R1&#xD;&#xA;	LastTransportVial = 	R1&#xD;&#xA;	TransportVialCapacity = 	10000&#xD;&#xA;	TransLiquidHeight = 	8.000 [mm]&#xD;&#xA;	TransVialPunctureDepth = 	5.000 [mm]&#xD;&#xA;	LoadingPump.Flow = 	20 [µl/min]&#xD;&#xA;	LoadingPump.%B = 	0.0 [%]&#xD;&#xA;	LoadingPump.%C = 	0.0 [%]&#xD;&#xA;	LoadingPump.Curve = 	5&#xD;&#xA;&#xD;&#xA; 0.000	Wait	UV.Ready and ColumnOven.Ready and Sampler.Ready&#xD;&#xA;	;Chromeleon sets this property to signal to Hystar that it is ready to start a run.&#xD;&#xA;	ReadyToRun = 	1&#xD;&#xA;	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	MicroPump.%B = 	4.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;	;Hystar sets this property to start the injection.&#xD;&#xA;	Wait	DoInject&#xD;&#xA;	Wait	UV.Ready and ColumnOven.Ready and Sampler.Ready&#xD;&#xA;	;Chromeleon sets this property to signal to Hystar, that it is ready to start a run.&#xD;&#xA;	Inject&#xD;&#xA;	;Chromeleon sets this property to signal the injection to Hystar.&#xD;&#xA;	InjectResponse = 	1&#xD;&#xA;	LoadingPump_Pressure.AcqOn&#xD;&#xA;	MicroPump_MasterPressure.AcqOn&#xD;&#xA;	ColumnOven_Temp.AcqOn&#xD;&#xA;	ColumnPressure.AcqOn&#xD;&#xA;	ColumnOven_FC_BridgeFlow.AcqOn&#xD;&#xA;	ColumnOven_FC_Stepper.AcqOn&#xD;&#xA;	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	ValveLeft = 	10_1&#xD;&#xA;	ValveRight = 	1_2&#xD;&#xA;	MicroPump.%B = 	4.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;&#xD;&#xA; 3.000	ValveLeft = 	1_2&#xD;&#xA;	ValveRight = 	1_2&#xD;&#xA;&#xD;&#xA;&#xD;&#xA;20.000	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	MicroPump.%B = 	70.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;&#xD;&#xA;20.100	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	MicroPump.%B = 	90.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;&#xD;&#xA;25.000	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	MicroPump.%B = 	90.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;&#xD;&#xA;25.100	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	MicroPump.%B = 	4.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;&#xD;&#xA;37.000	ValveLeft = 	10_1&#xD;&#xA;	ValveRight = 	1_2&#xD;&#xA;&#xD;&#xA;40.000	MicroPump.Flow = 	0.300 [µl/min]&#xD;&#xA;	MicroPump.%B = 	4.0 [%]&#xD;&#xA;	MicroPump.%C = 	0.0 [%]&#xD;&#xA;&#xD;&#xA;	LoadingPump_Pressure.AcqOff&#xD;&#xA;	MicroPump_MasterPressure.AcqOff&#xD;&#xA;	ColumnOven_Temp.AcqOff&#xD;&#xA;	ColumnPressure.AcqOff&#xD;&#xA;	ColumnOven_FC_BridgeFlow.AcqOff&#xD;&#xA;	ColumnOven_FC_Stepper.AcqOff&#xD;&#xA;&#xD;&#xA;	ReleaseExclusiveAccess	&#xD;&#xA;	End&#xD;&#xA;&#xD;&#xA;    &lt;/ModuleInfo&gt; &#xD;&#xA;&#xD;&#xA;&#xD;&#xA;&lt;/root&gt;" xmlns="" />
  <Device Type="None" Number="0" xmlns="" />
  <Device Type="Pump" Number="1" FullName="Dionex System	SYSTEM1	PUMP	PLUGIN" xmlns="" />
  <Device Type="AutoSampler" Number="1" FullName="Dionex System	SYSTEM1	ALS	PLUGIN" xmlns="" />
  <Device Type="Detector" Number="1" FullName="Dionex System	SYSTEM1	DET	PLUGIN" xmlns="" />
  <Device Type="System" Number="1" FullName="Dionex System	SYSTEM1	SYS	PLUGIN" xmlns="" />
  <Device Type="MassSpec" Number="1" FullName="HCT/esquire series	MS1	MS	LAN" xmlns="" />
</FractionTreatmentDataSet>