Raw file	Fraction	Enzyme	Enzyme mode	Enzyme first search	Enzyme mode first search	Use enzyme first search	Variable modifications	Fixed modifications	Multi modifications	Variable modifications first search	Use variable modifications first search	Requantify	Multiplicity	Max. missed cleavages	Labels0	LC-MS run type	Time-dependent recalibration	MS	MS/MS	MS3	MS/MS Submitted	MS/MS Submitted (SIL)	MS/MS Submitted (ISO)	MS/MS Submitted (PEAK)	MS/MS Identified	MS/MS Identified (SIL)	MS/MS Identified (ISO)	MS/MS Identified (PEAK)	MS/MS Identified [%]	MS/MS Identified (SIL) [%]	MS/MS Identified (ISO) [%]	MS/MS Identified (PEAK) [%]	Peptide Sequences Identified	Peaks	Peaks Sequenced	Peaks Sequenced [%]	Peaks Repeatedly Sequenced	Peaks Repeatedly Sequenced [%]	Isotope Patterns	Isotope Patterns Sequenced	Isotope Patterns Sequenced (z>1)	Isotope Patterns Sequenced [%]	Isotope Patterns Sequenced (z>1) [%]	Isotope Patterns Repeatedly Sequenced	Isotope Patterns Repeatedly Sequenced [%]	Recalibrated	Av. Absolute Mass Deviation [ppm]	Mass Standard Deviation [ppm]	Av. Absolute Mass Deviation [mDa]	Mass Standard Deviation [mDa]
TMT 1-phospho	1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		7454	22210	0	26059	18361	0	7698	1189	1134	0	55	4.56	6.18	0	0.71	951	3326397	18508	0.56	2859	15.45	399223	13754	13062	3.45	4.76	3301	24	+	1.421	1.9964	140560	149720
TMT1-1	2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		11008	24723	0	29433	20005	0	9428	2741	2445	0	296	9.31	12.22	0	3.14	1809	3256000	19556	0.6	4026	20.59	362782	13751	12749	3.79	7.21	4505	32.76	+	1.8329	2.498	118300	127330
TMT1-2	3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		10967	25909	0	30914	20904	0	10010	3457	3118	0	339	11.18	14.92	0	3.39	2314	3685198	21110	0.57	3871	18.34	415919	14826	13829	3.56	6.14	4427	29.86	+	1.711	2.3879	116200	125260
TMT1-3	4	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		9993	26992	0	31790	22194	0	9596	4759	4315	0	444	14.97	19.44	0	4.63	3166	3393972	22458	0.66	3761	16.75	377777	16154	15249	4.28	7.02	4549	28.16	+	1.6775	2.4172	134620	144340
TMT1-4	5	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		9245	29099	0	34164	24034	0	10130	5772	5252	0	520	16.89	21.85	0	5.13	3824	3182587	24224	0.76	4003	16.52	352588	17454	16531	4.95	7.74	4891	28.02	+	1.6358	2.3601	142310	151830
TMT1-5	6	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		8980	29580	0	34717	24443	0	10274	6328	5744	0	584	18.23	23.5	0	5.68	4132	2937146	24498	0.83	3990	16.29	330320	17695	16829	5.36	8.03	4854	27.43	+	1.5925	2.2616	148760	157990
TMT1-6	7	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		9144	28913	0	33726	24100	0	9626	6246	5716	0	530	18.52	23.72	0	5.51	4139	3085961	23808	0.77	4083	17.15	349725	17413	16546	4.98	7.46	4911	28.2	+	1.5705	2.3297	149700	159370
TMT1-7	8	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Acetyl (N-term)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		8695	29350	0	34288	24410	0	9878	5855	5364	0	491	17.08	21.97	0	4.97	3842	2944072	24280	0.82	4051	16.68	352245	17742	16855	5.04	7.18	4924	27.75	+	1.5152	2.1704	150830	160270
TMT1-8	9	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7961	31679	0	37209	26149	0	11060	6653	6104	0	549	17.88	23.34	0	4.96	4369	2166593	25888	1.19	4693	18.13	252252	18814	17776	7.46	11.3	5437	28.9	+	1.5623	2.3016	149710	159510
Total																		83447	248455	0	292300	204600	0	87700	43000	39192	0	3808	14.71	19.16	0	4.34	21272	27977926					3192831	147603	139426	4.62	7.22	41799	28.32		1.6073	2.3133	142280	152190