Raw file	Fraction	Enzyme	Enzyme mode	Enzyme first search	Enzyme mode first search	Use enzyme first search	Variable modifications	Fixed modifications	Multi modifications	Variable modifications first search	Use variable modifications first search	Requantify	Multiplicity	Max. missed cleavages	Labels0	LC-MS run type	Time-dependent recalibration	MS	MS/MS	MS3	MS/MS Submitted	MS/MS Submitted (SIL)	MS/MS Submitted (ISO)	MS/MS Submitted (PEAK)	MS/MS Identified	MS/MS Identified (SIL)	MS/MS Identified (ISO)	MS/MS Identified (PEAK)	MS/MS Identified [%]	MS/MS Identified (SIL) [%]	MS/MS Identified (ISO) [%]	MS/MS Identified (PEAK) [%]	Peptide Sequences Identified	Peaks	Peaks Sequenced	Peaks Sequenced [%]	Peaks Repeatedly Sequenced	Peaks Repeatedly Sequenced [%]	Isotope Patterns	Isotope Patterns Sequenced	Isotope Patterns Sequenced (z>1)	Isotope Patterns Sequenced [%]	Isotope Patterns Sequenced (z>1) [%]	Isotope Patterns Repeatedly Sequenced	Isotope Patterns Repeatedly Sequenced [%]	Recalibrated	Av. Absolute Mass Deviation [ppm]	Mass Standard Deviation [ppm]	Av. Absolute Mass Deviation [mDa]	Mass Standard Deviation [mDa]
F12+F24-169- HC_Hembras- TMT+AKTA- 17042019-12	1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		6374	33579	0	40801	26347	0	14454	4112	3747	0	365	10.08	14.22	NaN	2.53	2203	2679294	27470	1.03	3834	13.96	321275	18851	17437	5.87	8.91	4600	24.4	+	1.426	2.1882	134000	144050
F13-169- HC_Hembras- TMT+AKTA- 17042019-1	2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		10703	25984	0	32207	19759	0	12448	2200	2014	0	186	6.83	10.19	NaN	1.49	1574	4672244	23411	0.5	2160	9.23	480797	15433	14229	3.21	5.28	3281	21.26	+	1.542	2.177	106800	114700
F14-169- HC_Hembras- TMT+AKTA- 17042019-2	3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		10007	28975	0	35869	22081	0	13788	3388	3065	0	323	9.45	13.88	NaN	2.34	2509	4385285	26372	0.6	2236	8.48	467772	17432	16095	3.73	5.92	3623	20.78	+	1.6424	2.3791	115700	124380
F15-169- HC_Hembras- TMT+AKTA- 17042019-3	4	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		9604	31219	0	38713	23719	0	14994	4539	4134	0	405	11.72	17.43	NaN	2.7	3364	4230745	28472	0.67	2328	8.18	465636	18804	17514	4.04	6.31	3808	20.25	+	1.5261	2.2039	125680	134750
F16-169- HC_Hembras- TMT+AKTA- 17042019-4	5	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		9012	32563	0	39751	25367	0	14384	5466	4979	0	487	13.75	19.63	NaN	3.39	4069	4175444	29548	0.71	2476	8.38	477721	20154	18933	4.22	6.28	3986	19.78	+	1.5985	2.3586	133320	142250
F17-169- HC_Hembras- TMT+AKTA- 17042019-5	6	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		8254	34203	0	41897	26493	0	15404	5946	5367	0	579	14.19	20.26	NaN	3.76	4482	3778266	31157	0.82	2532	8.13	448684	21229	19932	4.73	6.85	4078	19.21	+	1.6926	2.4974	139440	148310
F18-169- HC_Hembras- TMT+AKTA- 17042019-6	7	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		8265	33541	0	40969	26099	0	14870	5754	5289	0	465	14.04	20.27	NaN	3.13	4341	3807348	30508	0.8	2555	8.37	453844	20933	19765	4.61	6.72	4031	19.26	+	1.6448	2.4104	145480	154450
F19-169- HC_Hembras- TMT+AKTA- 17042019-7	8	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		8318	32873	0	39645	26099	0	13546	5789	5265	0	524	14.6	20.17	NaN	3.87	4396	3771123	29749	0.79	2656	8.93	443602	20740	19555	4.68	6.91	4214	20.32	+	1.6213	2.4253	148110	157060
F20-169- HC_Hembras- TMT+AKTA- 17042019-8	9	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		7038	36524	0	43908	29122	0	14786	5564	5121	0	443	12.67	17.58	NaN	3	4123	2957978	32156	1.09	3410	10.6	360092	22466	21229	6.24	9.57	4917	21.89	+	1.5443	2.2572	151270	160380
F21-169- HC_Hembras- TMT+AKTA- 17042019-9	10	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		7220	34523	0	41437	27537	0	13900	4838	4413	0	425	11.68	16.03	NaN	3.06	3538	3033856	29986	0.99	3527	11.76	365988	20954	19699	5.73	8.91	4785	22.84	+	1.4747	2.1623	153440	162460
F22-169- HC_Hembras- TMT+AKTA- 17042019-10	11	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		7094	36692	0	44476	28876	0	15600	4453	4082	0	371	10.01	14.14	NaN	2.38	3264	3034773	32040	1.06	3641	11.36	371370	22096	20623	5.95	9.11	4882	22.09	+	1.4944	2.1586	152850	162010
F23-169- HC_Hembras- TMT+AKTA- 17042019-11	12	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		7244	29212	0	34760	23644	0	11116	3770	3457	0	313	10.85	14.62	NaN	2.82	2549	3134787	24945	0.8	3083	12.36	383067	17775	16694	4.64	7.15	4036	22.71	+	1.4534	2.1351	149260	158610
QC TMT-169- HC_Hembras- TMT+AKTA- 17042019-1	13	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Deamidation (N)	Carbamidomethyl (C)			False	False	1	1		Reporter ion MS2		6606	41416	0	51020	31812	0	19208	8070	7277	0	793	15.82	22.88	NaN	4.13	6315	2906195	38524	1.33	2418	6.28	365622	26440	24901	7.23	10.45	4270	16.15	+	1.6737	2.4345	143480	152870
Total																		105739	431304	0	525453	336955	0	188498	63889	58210	0	5679	12.16	17.28	NaN	3.01	22389	46567338					5405470	263307	246606	4.87	7.42	54511	20.7		1.5801	2.3204	140770	150320