Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS submitted MS/MS submitted (SIL) MS/MS submitted (ISO) MS/MS submitted (PEAK) MS/MS identified MS/MS identified (SIL) MS/MS identified (ISO) MS/MS identified (PEAK) MS/MS identified [%] MS/MS identified (SIL) [%] MS/MS identified (ISO) [%] MS/MS identified (PEAK) [%] Peptide sequences identified Peaks Peaks sequenced Peaks sequenced [%] Peaks repeatedly sequenced Peaks repeatedly sequenced [%] Isotope patterns Isotope patterns sequenced Isotope patterns sequenced (z>1) Isotope patterns sequenced [%] Isotope patterns sequenced (z>1) [%] Isotope patterns repeatedly sequenced Isotope patterns repeatedly sequenced [%] Recalibrated Av. absolute mass deviation [ppm] Mass standard deviation [ppm] Av. absolute mass deviation [mDa] Mass standard deviation [mDa] 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP343 1_#20_013_PAP343 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5405 23471 0 27052 19888 0 7164 1142 977 0 165 4.2 4.9 0 2.3 361 537151 21084 3.9 1182 5.6 70246 17725 17245 25 33 1593 9 + 0.37362 0.52845 0.22489 0.32243 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP344 1_#20_013_PAP344 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5840 29234 0 34070 24398 0 9672 1220 975 0 245 3.6 4 0 2.5 204 619485 25608 4.1 1379 5.4 79781 20854 20536 26 30 1985 9.5 + 0.46738 0.6116 0.32702 0.46157 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP345 1_#20_013_PAP345 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5925 30342 0 34881 25803 0 9078 1425 1240 0 185 4.1 4.8 0 2 247 600816 26661 4.4 1384 5.2 79110 21964 21734 28 32 2028 9.2 + 0.43125 0.56153 0.30869 0.43469 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP346 1_#20_013_PAP346 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 6162 22885 0 27479 18291 0 9188 747 641 0 106 2.7 3.5 0 1.2 220 623872 19921 3.2 1388 7 72489 15824 15072 22 31 1753 11 + 0.41587 0.5726 0.24345 0.34535 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP347 1_#20_013_PAP347 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5981 30745 0 35671 25819 0 9852 1363 1088 0 275 3.8 4.2 0 2.8 205 608524 26815 4.4 1528 5.7 79142 21775 21467 28 31 2205 10 + 0.48199 0.62729 0.34948 0.49062 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP348 1_#20_013_PAP348 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5251 25652 0 29565 21739 0 7826 656 614 0 42 2.2 2.8 0 0.54 309 567866 22551 4 1552 6.9 72566 18547 17975 26 31 2049 11 + 0.41826 0.57338 0.26858 0.37005 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP349 1_#20_013_PAP349 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5632 30675 0 35546 25804 0 9742 1182 970 0 212 3.3 3.8 0 2.2 173 610842 26737 4.4 1436 5.4 79546 21797 21529 27 31 2065 9.5 + 0.54624 0.72487 0.40958 0.58991 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP350 1_#20_013_PAP350 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5963 22579 0 26655 18503 0 8152 1219 987 0 232 4.6 5.3 0 2.8 346 550704 19548 3.5 1306 6.7 66469 15904 15388 24 34 1665 10 + 0.43737 0.57488 0.26176 0.35997 200227_C20_004_QHF1_PTM_Rev01_#20_013_PAP351 1_#20_013_PAP351 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5708 30286 0 35282 25290 0 9992 1555 1328 0 227 4.4 5.3 0 2.3 249 597637 26102 4.4 1600 6.1 80345 21272 20928 26 31 2050 9.6 + 0.43963 0.60481 0.30631 0.45155 200227_C20_004_QHF1_PTM_Rev04_#20_013_PAP347 4_#20_013_PAP347 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5910 29915 0 34159 25671 0 8488 1169 966 0 203 3.4 3.8 0 2.4 207 539634 26543 4.9 1306 4.9 75833 22180 21986 29 32 1925 8.7 + 0.43456 0.56201 0.30618 0.42433 200227_C20_004_QHF1_PTM_Rev04_#20_013_PAP351 4_#20_013_PAP351 Trypsin/P Specific Trypsin/P Specific True Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) True False 1 4 Standard 5829 28620 0 33172 24068 0 9104 1536 1291 0 245 4.6 5.4 0 2.7 264 498997 25014 5 1334 5.3 68721 20525 20280 30 33 1876 9.1 + 0.4787 0.64459 0.33732 0.48365 Total 63606 304404 0 353532 255274 0 98258 13214 11077 0 2137 3.7 4.3 0 2.2 594 6355528 824248 218367 214140 26 32 21194 9.7 0.44382 0.5968 0.29946 0.4304