Raw file	Experiment	Enzyme	Enzyme mode	Enzyme first search	Enzyme mode first search	Use enzyme first search	Variable modifications	Fixed modifications	Multi modifications	Variable modifications first search	Use variable modifications first search	Requantify	Multiplicity	Max. missed cleavages	Labels0	LC-MS run type	Time-dependent recalibration	MS	MS/MS	MS3	MS/MS submitted	MS/MS submitted (SIL)	MS/MS submitted (ISO)	MS/MS submitted (PEAK)	MS/MS identified	MS/MS identified (SIL)	MS/MS identified (ISO)	MS/MS identified (PEAK)	MS/MS identified [%]	MS/MS identified (SIL) [%]	MS/MS identified (ISO) [%]	MS/MS identified (PEAK) [%]	Peptide sequences identified	Peaks	Peaks sequenced	Peaks sequenced [%]	Peaks repeatedly sequenced	Peaks repeatedly sequenced [%]	Isotope patterns	Isotope patterns sequenced	Isotope patterns sequenced (z>1)	Isotope patterns sequenced [%]	Isotope patterns sequenced (z>1) [%]	Isotope patterns repeatedly sequenced	Isotope patterns repeatedly sequenced [%]	Recalibrated	Av. absolute mass deviation [ppm]	Mass standard deviation [ppm]	Av. absolute mass deviation [mDa]	Mass standard deviation [mDa]	Label free norm param
20210903_Cmix_2	Ctrl_2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Standard		11592	95863	0	120702	71024	0	49678	39318	35836	0	3482	33	50	0	7	24824	874037	73062	8.4	10066	14	129886	53094	52373	41	46	13785	26	+	0.61625	0.94387	0.44373	0.71194	
20210903_Tmix_1	Treated_1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Standard		11279	95787	0	119570	72004	0	47566	36303	33099	0	3204	30	46	0	6.7	23721	908280	73677	8.1	9666	13	138972	54593	53919	39	44	13499	25	+	0.63951	0.96974	0.45898	0.72777	
20210903_Tmix_3	Treated_3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Standard		11109	96540	0	120837	72243	0	48594	36334	33233	0	3101	30	46	0	6.4	23481	914573	74391	8.1	9658	13	139597	54810	54098	39	44	13475	25	+	0.6498	0.98477	0.46886	0.74292	
20210903_Cmix_1	Ctrl_1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Standard		11458	96668	0	121589	71747	0	49842	39512	36024	0	3488	32	50	0	7	24946	879340	73645	8.4	10251	14	131104	53657	52956	41	46	13950	26	+	0.61381	0.94752	0.44299	0.71722	
20210903_Cmix_3	Ctrl_3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Standard		11670	95916	0	120612	71220	0	49392	39348	36001	0	3347	33	51	0	6.8	24904	871993	73267	8.4	9909	14	129344	53291	52568	41	46	13800	26	+	0.62503	0.95417	0.45072	0.72155	
20210903_Tmix_2	Treated_2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Standard		11335	95826	0	120047	71605	0	48442	36206	33114	0	3092	30	46	0	6.4	23378	904315	73677	8.1	9660	13	137715	54163	53475	39	44	13442	25	+	0.64585	0.97761	0.46512	0.73773	
Total																		68443	576600	0	723357	429843	0	293514	227021	207307	0	19714	31	48	0	6.7	37761	5352538					806618	323608	319389	40	45	81951	25		0.6313	0.96263	0.45478	0.72631