MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data110719_BSA10fmol_2050V_5.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	tr|B4F7C2|B4F7C2_RAT	Tubulin beta chain OS=Rattus norvegicus (Rat) OX=10116 GN=Tubb4a PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23.0	null	263-UNIMOD:730,267-UNIMOD:35	0.03	23.0	2	1	0
PRT	sp|P34058|HS90B_RAT	Heat shock protein HSP 90-beta OS=Rattus norvegicus (Rat) OX=10116 GN=Hsp90ab1 PE=1 SV=4	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19.0	null	412-UNIMOD:730,412-UNIMOD:39,423-UNIMOD:730,427-UNIMOD:730	0.02	19.0	1	1	1
PRT	sp|B5DF45|TRAF6_RAT	TNF receptor-associated factor 6 OS=Rattus norvegicus (Rat) OX=10116 GN=Traf6 PE=2 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	12.0	null	401-UNIMOD:730	0.02	12.0	1	1	1
PRT	tr|A0A0G2K666|A0A0G2K666_RAT	Isoform of Q9JK96, Potassium voltage-gated channel subfamily KQT member 4 OS=Rattus norvegicus (Rat) OX=10116 GN=Kcnq4 PE=3 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	12.0	null	401-UNIMOD:730	0.02	12.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	LHFFMPGFAPLTSR	1	tr|B4F7C2|B4F7C2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23	1-UNIMOD:730,5-UNIMOD:35	ms_run[1]:scan=1.1.2367.2	55.13115	3	1940.076971	1940.028529	R	G	263	277
PSM	LHFFMPGFAPLTSR	2	tr|B4F7C2|B4F7C2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21	1-UNIMOD:730	ms_run[1]:scan=1.1.2797.2	63.38103	3	1924.103471	1924.033614	R	G	263	277
PSM	CLELFSELAEDKENYK	3	sp|P34058|HS90B_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:730,1-UNIMOD:39,12-UNIMOD:730,16-UNIMOD:730	ms_run[1]:scan=1.1.3524.2	76.38988	4	2888.570094	2888.506796	K	K	412	428
PSM	LHLQLPTAQR	4	sp|B5DF45|TRAF6_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	12	1-UNIMOD:730	ms_run[1]:scan=1.1.2037.2	48.89072	2	1478.836247	1479.882851	R	C	401	411
PSM	RAPVPDGAASR	5	tr|A0A0G2K666|A0A0G2K666_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	12	1-UNIMOD:730	ms_run[1]:scan=1.1.2590.3	59.52688	2	1398.728447	1399.783865	R	Y	401	412