MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data1108011_PC12_C15_2100V_120min_10.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	sp|Q9QXU9|PCS1N_RAT	ProSAAS OS=Rattus norvegicus (Rat) OX=10116 GN=Pcsk1n PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	37.0	null	118-UNIMOD:730	0.08	37.0	1	1	1
PRT	sp|P05065|ALDOA_RAT	Fructose-bisphosphate aldolase A OS=Rattus norvegicus (Rat) OX=10116 GN=Aldoa PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	35.0	null	112-UNIMOD:730,332-UNIMOD:730,339-UNIMOD:39,342-UNIMOD:730	0.10	35.0	4	2	1
PRT	tr|C0JPT7|C0JPT7_RAT	Filamin A OS=Rattus norvegicus (Rat) OX=10116 GN=Flna PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	33.0	null	1492-UNIMOD:730	0.01	33.0	1	1	1
PRT	sp|P63039|CH60_RAT	60 kDa heat shock protein, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Hspd1 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	33.0	null	97-UNIMOD:730	0.05	33.0	1	1	1
PRT	tr|Q6AYR1|Q6AYR1_RAT	PB1 domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Tfg PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	32.0	null	119-UNIMOD:730	0.07	32.0	1	1	1
PRT	sp|P10719|ATPB_RAT	ATP synthase subunit beta, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Atp5f1b PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23.0	null	325-UNIMOD:730,339-UNIMOD:35,342-UNIMOD:35	0.04	23.0	1	1	1
PRT	tr|Q5U214|Q5U214_RAT	Small nuclear ribonucleoprotein polypeptide A OS=Rattus norvegicus (Rat) OX=10116 GN=Snrpa PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22.0	null	124-UNIMOD:730,143-UNIMOD:35,146-UNIMOD:35	0.10	22.0	1	1	1
PRT	sp|P52873|PYC_RAT	Pyruvate carboxylase, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Pc PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21.0	null	520-UNIMOD:730	0.02	21.0	1	1	1
PRT	sp|P62815|VATB2_RAT	V-type proton ATPase subunit B, brain isoform OS=Rattus norvegicus (Rat) OX=10116 GN=Atp6v1b2 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ],[MS, MS:1001476, X!Tandem, ]	20.0	null	8-UNIMOD:730,25-UNIMOD:35	0.05	20.0	3	1	0
PRT	tr|D3ZTR5|D3ZTR5_RAT	DUF4371 domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Zbed5 PE=4 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20.0	null	29-UNIMOD:730	0.03	20.0	1	1	1
PRT	sp|P05197|EF2_RAT	Elongation factor 2 OS=Rattus norvegicus (Rat) OX=10116 GN=Eef2 PE=1 SV=4	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19.0	null	581-UNIMOD:730,591-UNIMOD:39,594-UNIMOD:730	0.02	19.0	1	1	1
PRT	tr|F1LZI1|F1LZI1_RAT	Similar to heat shock protein 8 OS=Rattus norvegicus (Rat) OX=10116 GN=LOC680121 PE=3 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	138-UNIMOD:730	0.03	17.0	3	1	0
PRT	tr|D4AAM0|D4AAM0_RAT	Xpo1 domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Tnpo3 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	595-UNIMOD:730	0.02	17.0	1	1	1
PRT	sp|P60711|ACTB_RAT	Actin, cytoplasmic 1 OS=Rattus norvegicus (Rat) OX=10116 GN=Actb PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ],[MS, MS:1001476, X!Tandem, ]	16.0	null	292-UNIMOD:730,305-UNIMOD:35,360-UNIMOD:28	0.10	16.0	2	2	2
PRT	sp|Q6P4Z9|CSN8_RAT	COP9 signalosome complex subunit 8 OS=Rattus norvegicus (Rat) OX=10116 GN=Cops8 PE=2 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	179-UNIMOD:730	0.11	15.0	1	1	1
PRT	sp|P28023|DCTN1_RAT	Dynactin subunit 1 OS=Rattus norvegicus (Rat) OX=10116 GN=Dctn1 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1001476, X!Tandem, ]	14.0	null	1049-UNIMOD:730	0.02	14.0	1	1	1
PRT	tr|F1LQP9|F1LQP9_RAT	Importin N-terminal domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Tnpo1 PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	884-UNIMOD:730	0.01	14.0	2	1	0
PRT	sp|P62738|ACTA_RAT	Actin, aortic smooth muscle OS=Rattus norvegicus (Rat) OX=10116 GN=Acta2 PE=2 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	362-UNIMOD:730	0.04	14.0	1	1	1
PRT	sp|P11348|DHPR_RAT	Dihydropteridine reductase OS=Rattus norvegicus (Rat) OX=10116 GN=Qdpr PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	165-UNIMOD:730,168-UNIMOD:35,186-UNIMOD:35	0.10	14.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	ASDPPLAPDDDPDAPAAQLAR	1	sp|Q9QXU9|PCS1N_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	37	1-UNIMOD:730	ms_run[1]:scan=1.1.1841.2	33.19602	3	2406.129971	2406.196987	R	A	118	139
PSM	GVVPLAGTNGETTTQGLDGLSER	2	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	35	1-UNIMOD:730	ms_run[1]:scan=1.1.1975.2	43.23222	3	2576.223071	2575.339629	K	C	112	135
PSM	GLVEPVDVVDNADGTQTVNYVPSR	3	tr|C0JPT7|C0JPT7_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	33	1-UNIMOD:730	ms_run[1]:scan=1.1.2025.2	47.51235	3	2847.356771	2847.455721	K	E	1492	1516
PSM	LVQDVANNTNEEAGDGTTTATVLAR	4	sp|P63039|CH60_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	33	1-UNIMOD:730	ms_run[1]:scan=1.1.1981.2	43.87963	3	2863.358771	2863.446613	K	S	97	122
PSM	LLDSLEPPGEPGPSTSIPENDTVDGR	5	tr|Q6AYR1|Q6AYR1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	32	1-UNIMOD:730	ms_run[1]:scan=1.1.2279.4	55.56593	3	2995.379171	2995.492895	R	E	119	145
PSM	IPSAVGYQPTLATDMGTMQER	6	sp|P10719|ATPB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23	1-UNIMOD:730,15-UNIMOD:35,18-UNIMOD:35	ms_run[1]:scan=1.1.1881.2	36.77098	3	2601.192671	2601.272144	R	I	325	346
PSM	GVVPLAGTNGETTTQGLDGLSER	7	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22	1-UNIMOD:730	ms_run[1]:scan=1.1.1986.3	44.40413	3	2576.223071	2575.339629	K	C	112	135
PSM	AVQGGAAAPVVGAVQPVPGMPPMTQAPR	8	tr|Q5U214|Q5U214_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22	1-UNIMOD:730,20-UNIMOD:35,23-UNIMOD:35	ms_run[1]:scan=1.1.1847.4	33.78527	4	2989.490094	2989.578437	K	I	124	152
PSM	VSPSPVDPIVPVVPIGPPPAGFR	9	sp|P52873|PYC_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21	1-UNIMOD:730	ms_run[1]:scan=1.1.2690.2	65.51255	3	2597.397071	2597.488800	K	D	520	543
PSM	GVVPLAGTNGETTTQGLDGLSER	10	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1876.4	36.33288	4	2576.259294	2575.339629	K	C	112	135
PSM	GIVNGAAPELPVPTGGPMAGAR	11	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.2028.3	47.78176	3	2336.180471	2335.262505	R	E	8	30
PSM	APAAQPPATAAAPSAVGSPAAAPR	12	tr|D3ZTR5|D3ZTR5_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1737.3	23.3324	3	2401.241471	2401.302061	R	Q	29	53
PSM	ETVSEESNVLCLSK	13	sp|P05197|EF2_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:730,11-UNIMOD:39,14-UNIMOD:730	ms_run[1]:scan=1.1.1919.3	39.46158	3	2193.087971	2191.132580	R	S	581	595
PSM	GIVNGAAPELPVPTGGPMAGAR	14	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1001476, X!Tandem, ]	17	1-UNIMOD:730,18-UNIMOD:35	ms_run[1]:scan=1.1.1964.2	42.53837	3	2352.1902	2351.2572	R	E	8	30
PSM	TVTNAVVTVPAYFNDSQR	15	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2031.3	48.01202	3	2286.103271	2285.195865	K	Q	138	156
PSM	TVTNAVVTVPAYFNDSQR	16	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2015.2	46.97692	3	2286.153371	2285.195865	K	Q	138	156
PSM	LLSQEPSNGISSDPTVFLDR	17	tr|D4AAM0|D4AAM0_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2388.2	58.34215	3	2479.291871	2478.290888	K	L	595	615
PSM	DLYANTVLSGGTTMYPGIADR	18	sp|P60711|ACTB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730,14-UNIMOD:35	ms_run[1]:scan=1.1.1992.4	45.06208	3	2535.163271	2534.262959	K	M	292	313
PSM	FIPLSEPAPVPPIPNEQQLAR	19	sp|Q6P4Z9|CSN8_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.2455.2	60.64983	3	2616.379271	2616.458228	R	L	179	200
PSM	GIVNGAAPELPVPTGGPMAGAR	20	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730,18-UNIMOD:35	ms_run[1]:scan=1.1.1933.2	40.89068	3	2352.156971	2351.257420	R	E	8	30
PSM	QEYDESGPSIVHR	21	sp|P60711|ACTB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1001476, X!Tandem, ]	15	1-UNIMOD:28	ms_run[1]:scan=1.1.1710.2	20.82105	3	1802.8362	1802.8732	K	K	360	373
PSM	TVTNAVVTVPAYFNDSQR	22	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.2004.4	46.18375	3	2287.097771	2285.195865	K	Q	138	156
PSM	ALANSLACQGK	23	sp|P05065|ALDOA_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,8-UNIMOD:39,11-UNIMOD:730	ms_run[1]:scan=1.1.1779.4	27.28445	3	1730.893871	1728.947620	R	Y	332	343
PSM	GPPPSGIATLVSGIAGEEQQR	24	sp|P28023|DCTN1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1001476, X!Tandem, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1937.4	41.27728	3	2369.1692	2367.2692	R	G	1049	1070
PSM	LAAFYGV	25	tr|F1LQP9|F1LQP9_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.2588.2	63.5386	2	1043.560247	1043.595837	R	-	884	891
PSM	LAAFYGV	26	tr|F1LQP9|F1LQP9_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.2656.3	64.61996	2	1043.560247	1043.595837	R	-	884	891
PSM	QEYDEAGPSIVHR	27	sp|P62738|ACTA_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1710.2	20.82105	3	1802.837471	1803.905831	K	K	362	375
PSM	NSGMPSGAAAIAVLPVTLDTPMNR	28	sp|P11348|DHPR_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,4-UNIMOD:35,22-UNIMOD:35	ms_run[1]:scan=1.1.2175.2	51.65997	3	2718.312671	2718.398741	K	K	165	189