MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data1108012_PC12_C13_2100V_120min_11.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	tr|Q5U214|Q5U214_RAT	Small nuclear ribonucleoprotein polypeptide A OS=Rattus norvegicus (Rat) OX=10116 GN=Snrpa PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	29.0	null	124-UNIMOD:730,143-UNIMOD:35,146-UNIMOD:35	0.10	29.0	1	1	1
PRT	sp|Q9QXU9|PCS1N_RAT	ProSAAS OS=Rattus norvegicus (Rat) OX=10116 GN=Pcsk1n PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	28.0	null	118-UNIMOD:730	0.08	28.0	1	1	1
PRT	sp|P05065|ALDOA_RAT	Fructose-bisphosphate aldolase A OS=Rattus norvegicus (Rat) OX=10116 GN=Aldoa PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23.0	null	112-UNIMOD:730	0.07	23.0	3	1	0
PRT	sp|P10719|ATPB_RAT	ATP synthase subunit beta, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Atp5f1b PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	325-UNIMOD:730,339-UNIMOD:35,342-UNIMOD:35	0.04	18.0	1	1	1
PRT	tr|F1LZI1|F1LZI1_RAT	Similar to heat shock protein 8 OS=Rattus norvegicus (Rat) OX=10116 GN=LOC680121 PE=3 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	138-UNIMOD:730	0.03	17.0	2	1	0
PRT	sp|P62815|VATB2_RAT	V-type proton ATPase subunit B, brain isoform OS=Rattus norvegicus (Rat) OX=10116 GN=Atp6v1b2 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	8-UNIMOD:730,25-UNIMOD:35	0.05	15.0	2	1	0
PRT	tr|F1LQP9|F1LQP9_RAT	Importin N-terminal domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Tnpo1 PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	884-UNIMOD:730	0.01	15.0	1	1	1
PRT	sp|P51650|SSDH_RAT	Succinate-semialdehyde dehydrogenase, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Aldh5a1 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	517-UNIMOD:730,519-UNIMOD:39	0.02	14.0	1	1	1
PRT	tr|D4A628|D4A628_RAT	DNA polymerase theta OS=Rattus norvegicus (Rat) OX=10116 GN=Polq PE=4 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	12.0	null	1-UNIMOD:730,1-UNIMOD:35	0.00	12.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	AVQGGAAAPVVGAVQPVPGMPPMTQAPR	1	tr|Q5U214|Q5U214_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	29	1-UNIMOD:730,20-UNIMOD:35,23-UNIMOD:35	ms_run[1]:scan=1.1.1781.4	33.43093	4	2989.498494	2989.578437	K	I	124	152
PSM	ASDPPLAPDDDPDAPAAQLAR	2	sp|Q9QXU9|PCS1N_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	28	1-UNIMOD:730	ms_run[1]:scan=1.1.1777.3	32.9955	3	2406.127871	2406.196987	R	A	118	139
PSM	GVVPLAGTNGETTTQGLDGLSER	3	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23	1-UNIMOD:730	ms_run[1]:scan=1.1.2079.2	43.08813	3	2576.247971	2575.339629	K	C	112	135
PSM	IPSAVGYQPTLATDMGTMQER	4	sp|P10719|ATPB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730,15-UNIMOD:35,18-UNIMOD:35	ms_run[1]:scan=1.1.1832.3	36.47721	3	2601.184571	2601.272144	R	I	325	346
PSM	GVVPLAGTNGETTTQGLDGLSER	5	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.1828.2	36.0898	4	2576.293694	2575.339629	K	C	112	135
PSM	TVTNAVVTVPAYFNDSQR	6	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2197.3	47.51343	3	2286.113171	2285.195865	K	Q	138	156
PSM	GIVNGAAPELPVPTGGPMAGAR	7	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730,18-UNIMOD:35	ms_run[1]:scan=1.1.2060.2	42.32337	3	2352.169871	2351.257420	R	E	8	30
PSM	TVTNAVVTVPAYFNDSQR	8	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.2134.3	46.12925	3	2287.107371	2285.195865	K	Q	138	156
PSM	LAAFYGV	9	tr|F1LQP9|F1LQP9_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.3174.2	63.51928	2	1043.561047	1043.595837	R	-	884	891
PSM	GVVPLAGTNGETTTQGLDGLSER	10	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1826.3	35.94647	4	2576.293694	2575.339629	K	C	112	135
PSM	GIVNGAAPELPVPTGGPMAGAR	11	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,18-UNIMOD:35	ms_run[1]:scan=1.1.2002.4	40.76318	3	2352.166271	2351.257420	R	E	8	30
PSM	YVCYGGL	12	sp|P51650|SSDH_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,3-UNIMOD:39	ms_run[1]:scan=1.1.2627.2	55.56395	2	1122.545447	1123.534893	K	-	517	524
PSM	MSLPRR	13	tr|D4A628|D4A628_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	12	1-UNIMOD:730,1-UNIMOD:35	ms_run[1]:scan=1.1.1766.4	31.98575	2	1078.579647	1078.622403	-	S	1	7