MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data1108012_PC12_F6_2100V_120min_7.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	sp|Q01992|MIPEP_RAT	Mitochondrial intermediate peptidase OS=Rattus norvegicus (Rat) OX=10116 GN=Mipep PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	24.0	null	230-UNIMOD:730	0.02	24.0	1	1	1
PRT	sp|P00762|TRY1_RAT	Anionic trypsin-1 OS=Rattus norvegicus (Rat) OX=10116 GN=Prss1 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ],[MS, MS:1001476, X!Tandem, ]	21.0	null	73-UNIMOD:730,92-UNIMOD:730	0.09	21.0	2	1	0
PRT	sp|Q9JM53|AIFM1_RAT	Apoptosis-inducing factor 1, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Aifm1 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20.0	null	450-UNIMOD:730	0.02	20.0	1	1	1
PRT	sp|P10818|CX6A1_RAT	Cytochrome c oxidase subunit 6A1, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Cox6a1 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20.0	null	65-UNIMOD:730	0.15	20.0	1	1	1
PRT	sp|O08722|UNC5B_RAT	Netrin receptor UNC5B OS=Rattus norvegicus (Rat) OX=10116 GN=Unc5b PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20.0	null	756-UNIMOD:730	0.01	20.0	2	1	0
PRT	sp|P46892|CD11B_RAT	Cyclin-dependent kinase 11B OS=Rattus norvegicus (Rat) OX=10116 GN=Cdk11b PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19.0	null	193-UNIMOD:730	0.03	19.0	1	1	1
PRT	sp|P20156|VGF_RAT	Neurosecretory protein VGF OS=Rattus norvegicus (Rat) OX=10116 GN=Vgf PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	567-UNIMOD:730	0.02	18.0	1	1	1
PRT	sp|P83871|PHF5A_RAT	PHD finger-like domain-containing protein 5A OS=Rattus norvegicus (Rat) OX=10116 GN=Phf5a PE=2 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	4-UNIMOD:730,11-UNIMOD:39	0.09	18.0	1	1	1
PRT	sp|Q63014|AKAP8_RAT	A-kinase anchor protein 8 OS=Rattus norvegicus (Rat) OX=10116 GN=Akap8 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	496-UNIMOD:730	0.02	18.0	1	1	1
PRT	tr|D3ZFF4|D3ZFF4_RAT	PlsC domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Lclat1 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	242-UNIMOD:730	0.02	18.0	1	1	1
PRT	sp|P60711|ACTB_RAT	Actin, cytoplasmic 1 OS=Rattus norvegicus (Rat) OX=10116 GN=Actb PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	85-UNIMOD:730	0.03	18.0	1	1	1
PRT	sp|O35314|SCG1_RAT	Secretogranin-1 OS=Rattus norvegicus (Rat) OX=10116 GN=Chgb PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	324-UNIMOD:730	0.01	17.0	1	1	1
PRT	tr|G3V8A5|G3V8A5_RAT	Vacuolar protein sorting-associated protein 35 OS=Rattus norvegicus (Rat) OX=10116 GN=Vps35 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	769-UNIMOD:730	0.01	17.0	1	1	1
PRT	tr|D4AAH9|D4AAH9_RAT	TBC1 domain family, member 23 OS=Rattus norvegicus (Rat) OX=10116 GN=Tbc1d23 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16.0	null	165-UNIMOD:730	0.01	16.0	1	1	1
PRT	sp|Q498E0|TXD12_RAT	Thioredoxin domain-containing protein 12 OS=Rattus norvegicus (Rat) OX=10116 GN=Txndc12 PE=2 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16.0	null	31-UNIMOD:730	0.06	16.0	1	1	1
PRT	tr|B4F7C2|B4F7C2_RAT	Tubulin beta chain OS=Rattus norvegicus (Rat) OX=10116 GN=Tubb4a PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	263-UNIMOD:730,267-UNIMOD:35	0.03	15.0	1	1	1
PRT	tr|D4ABI7|D4ABI7_RAT	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Rattus norvegicus (Rat) OX=10116 GN=Hacd3 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	86-UNIMOD:730	0.02	15.0	1	1	1
PRT	tr|D4AD36|D4AD36_RAT	FCH and double SH3 domains 1 OS=Rattus norvegicus (Rat) OX=10116 GN=Fchsd1 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	342-UNIMOD:730	0.01	15.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	HLLPEHIQHHFAR	1	sp|Q01992|MIPEP_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	24	1-UNIMOD:730	ms_run[1]:scan=1.1.1318.3	21.08682	4	1938.044894	1938.064338	K	D	230	243
PSM	LGEHNINVLEGDEQFINAAK	2	sp|P00762|TRY1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21	1-UNIMOD:730,20-UNIMOD:730	ms_run[1]:scan=1.1.1720.2	46.28528	4	2818.428094	2818.507481	R	I	73	93
PSM	RVEHHDHAVVSGR	3	sp|Q9JM53|AIFM1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1239.2	14.75733	4	1801.937294	1801.960266	R	L	450	463
PSM	HEEHERPEFVAYPHLR	4	sp|P10818|CX6A1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1335.2	22.56205	4	2349.159294	2349.192117	R	I	65	81
PSM	LSLHDIPHAHWR	5	sp|O08722|UNC5B_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1417.2	30.4463	4	1784.951694	1784.974126	R	S	756	768
PSM	HLHDNWILHR	6	sp|P46892|CD11B_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:730	ms_run[1]:scan=1.1.1343.2	23.37685	4	1643.874894	1643.895147	K	D	193	203
PSM	LGEHNINVLEGDEQFINAAK	7	sp|P00762|TRY1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1001476, X!Tandem, ]	19	1-UNIMOD:730,20-UNIMOD:730	ms_run[1]:scan=1.1.1719.2	46.20873	4	2818.4282	2818.5072	R	I	73	93
PSM	HFHHALPPAR	8	sp|P20156|VGF_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730	ms_run[1]:scan=1.1.1274.3	17.05945	3	1485.794171	1485.826005	R	H	567	577
PSM	HHPDLIFCR	9	sp|P83871|PHF5A_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730,8-UNIMOD:39	ms_run[1]:scan=1.1.1438.2	32.2757	3	1486.717571	1486.748014	K	K	4	13
PSM	HLHSVDHNHNR	10	sp|Q63014|AKAP8_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730	ms_run[1]:scan=1.1.1228.3	13.83717	4	1668.833294	1668.849988	R	R	496	507
PSM	EIHFHVHR	11	tr|D3ZFF4|D3ZFF4_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730	ms_run[1]:scan=1.1.1296.2	18.83538	3	1377.738671	1377.757256	K	Y	242	250
PSM	IWHHTFYNELR	12	sp|P60711|ACTB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730	ms_run[1]:scan=1.1.1463.2	34.27282	4	1818.912894	1818.947242	K	V	85	96
PSM	GHHLAHYR	13	sp|O35314|SCG1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.1244.2	15.11327	3	1293.671171	1293.699742	R	A	324	332
PSM	HFHNTLEHLR	14	tr|G3V8A5|G3V8A5_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.1307.3	19.94482	4	1606.844494	1606.863512	K	S	769	779
PSM	GRPFHLFR	15	tr|D4AAH9|D4AAH9_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730	ms_run[1]:scan=1.1.1368.2	25.89547	3	1332.742571	1332.772178	K	L	165	173
PSM	GFGDHIHWR	16	sp|Q498E0|TXD12_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730	ms_run[1]:scan=1.1.1361.2	25.16312	3	1427.709971	1427.736521	K	T	31	40
PSM	LHFFMPGFAPLTSR	17	tr|B4F7C2|B4F7C2_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730,5-UNIMOD:35	ms_run[1]:scan=1.1.2245.2	54.01575	3	1939.982471	1940.028529	R	G	263	277
PSM	GSHWWER	18	tr|D4ABI7|D4ABI7_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.1345.2	23.55818	3	1260.616271	1260.630659	K	L	86	93
PSM	LSLHDIPHAHWR	19	sp|O08722|UNC5B_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.1416.4	30.40218	4	1784.951694	1784.974126	R	S	756	768
PSM	IQHHGHR	20	tr|D4AD36|D4AD36_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.1200.2	11.89608	2	1187.612647	1187.657877	K	V	342	349