MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data110815_PC12_C14_2000V_120min_9.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	sp|Q9QXU9|PCS1N_RAT	ProSAAS OS=Rattus norvegicus (Rat) OX=10116 GN=Pcsk1n PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	38.0	null	118-UNIMOD:730	0.08	38.0	1	1	1
PRT	sp|P63039|CH60_RAT	60 kDa heat shock protein, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Hspd1 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	37.0	null	97-UNIMOD:730	0.05	37.0	2	1	0
PRT	sp|P05065|ALDOA_RAT	Fructose-bisphosphate aldolase A OS=Rattus norvegicus (Rat) OX=10116 GN=Aldoa PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	34.0	null	112-UNIMOD:730,332-UNIMOD:730,339-UNIMOD:39,342-UNIMOD:730	0.10	34.0	5	2	1
PRT	tr|D3ZTR5|D3ZTR5_RAT	DUF4371 domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Zbed5 PE=4 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	31.0	null	29-UNIMOD:730	0.03	31.0	1	1	1
PRT	sp|P10719|ATPB_RAT	ATP synthase subunit beta, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Atp5f1b PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	28.0	null	325-UNIMOD:730,339-UNIMOD:35,342-UNIMOD:35	0.04	28.0	1	1	1
PRT	tr|Q5U214|Q5U214_RAT	Small nuclear ribonucleoprotein polypeptide A OS=Rattus norvegicus (Rat) OX=10116 GN=Snrpa PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	27.0	null	124-UNIMOD:730,143-UNIMOD:35,146-UNIMOD:35	0.10	27.0	1	1	1
PRT	tr|C0JPT7|C0JPT7_RAT	Filamin A OS=Rattus norvegicus (Rat) OX=10116 GN=Flna PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	27.0	null	1492-UNIMOD:730	0.01	27.0	1	1	1
PRT	sp|P04177|TY3H_RAT	Tyrosine 3-monooxygenase OS=Rattus norvegicus (Rat) OX=10116 GN=Th PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	25.0	null	135-UNIMOD:730	0.03	25.0	1	1	1
PRT	sp|P11348|DHPR_RAT	Dihydropteridine reductase OS=Rattus norvegicus (Rat) OX=10116 GN=Qdpr PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	24.0	null	165-UNIMOD:730,168-UNIMOD:35,186-UNIMOD:35	0.10	24.0	1	1	1
PRT	sp|Q6P4Z9|CSN8_RAT	COP9 signalosome complex subunit 8 OS=Rattus norvegicus (Rat) OX=10116 GN=Cops8 PE=2 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23.0	null	179-UNIMOD:730	0.11	23.0	1	1	1
PRT	sp|P52873|PYC_RAT	Pyruvate carboxylase, mitochondrial OS=Rattus norvegicus (Rat) OX=10116 GN=Pc PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22.0	null	520-UNIMOD:730	0.02	22.0	2	1	0
PRT	sp|P62815|VATB2_RAT	V-type proton ATPase subunit B, brain isoform OS=Rattus norvegicus (Rat) OX=10116 GN=Atp6v1b2 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ],[MS, MS:1001476, X!Tandem, ]	21.0	null	8-UNIMOD:730,25-UNIMOD:35	0.05	21.0	3	1	0
PRT	tr|Q6AYR1|Q6AYR1_RAT	PB1 domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Tfg PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21.0	null	119-UNIMOD:730	0.07	21.0	1	1	1
PRT	tr|F1LZI1|F1LZI1_RAT	Similar to heat shock protein 8 OS=Rattus norvegicus (Rat) OX=10116 GN=LOC680121 PE=3 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	138-UNIMOD:730	0.03	17.0	2	1	0
PRT	sp|P05197|EF2_RAT	Elongation factor 2 OS=Rattus norvegicus (Rat) OX=10116 GN=Eef2 PE=1 SV=4	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	581-UNIMOD:730,591-UNIMOD:39,594-UNIMOD:730	0.02	17.0	1	1	1
PRT	sp|P85515|ACTZ_RAT	Alpha-centractin OS=Rattus norvegicus (Rat) OX=10116 GN=Actr1a PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	183-UNIMOD:730	0.02	14.0	1	1	1
PRT	tr|D4A4P8|D4A4P8_RAT	Protein tweety homolog OS=Rattus norvegicus (Rat) OX=10116 GN=Ttyh2 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	4-UNIMOD:730	0.02	14.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	ASDPPLAPDDDPDAPAAQLAR	1	sp|Q9QXU9|PCS1N_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	38	1-UNIMOD:730	ms_run[1]:scan=1.1.1836.2	32.18245	3	2406.115271	2406.196987	R	A	118	139
PSM	LVQDVANNTNEEAGDGTTTATVLAR	2	sp|P63039|CH60_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	37	1-UNIMOD:730	ms_run[1]:scan=1.1.1968.4	43.2605	3	2863.366571	2863.446613	K	S	97	122
PSM	GVVPLAGTNGETTTQGLDGLSER	3	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	34	1-UNIMOD:730	ms_run[1]:scan=1.1.1962.2	42.59428	3	2576.235371	2575.339629	K	C	112	135
PSM	APAAQPPATAAAPSAVGSPAAAPR	4	tr|D3ZTR5|D3ZTR5_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	31	1-UNIMOD:730	ms_run[1]:scan=1.1.1727.4	22.64168	3	2401.224671	2401.302061	R	Q	29	53
PSM	IPSAVGYQPTLATDMGTMQER	5	sp|P10719|ATPB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	28	1-UNIMOD:730,15-UNIMOD:35,18-UNIMOD:35	ms_run[1]:scan=1.1.1879.4	35.8158	3	2601.191771	2601.272144	R	I	325	346
PSM	GVVPLAGTNGETTTQGLDGLSER	6	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	27	1-UNIMOD:730	ms_run[1]:scan=1.1.1871.3	35.1554	3	2576.227571	2575.339629	K	C	112	135
PSM	AVQGGAAAPVVGAVQPVPGMPPMTQAPR	7	tr|Q5U214|Q5U214_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	27	1-UNIMOD:730,20-UNIMOD:35,23-UNIMOD:35	ms_run[1]:scan=1.1.1842.2	32.63887	4	2989.512494	2989.578437	K	I	124	152
PSM	GLVEPVDVVDNADGTQTVNYVPSR	8	tr|C0JPT7|C0JPT7_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	27	1-UNIMOD:730	ms_run[1]:scan=1.1.2019.3	47.01553	3	2847.354371	2847.455721	K	E	1492	1516
PSM	FEVPSGDLAALLSSVR	9	sp|P04177|TY3H_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	25	1-UNIMOD:730	ms_run[1]:scan=1.1.3611.2	76.80705	3	1963.997771	1964.088546	R	R	135	151
PSM	GVVPLAGTNGETTTQGLDGLSER	10	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	24	1-UNIMOD:730	ms_run[1]:scan=1.1.1977.3	43.8191	3	2576.235371	2575.339629	K	C	112	135
PSM	LVQDVANNTNEEAGDGTTTATVLAR	11	sp|P63039|CH60_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	24	1-UNIMOD:730	ms_run[1]:scan=1.1.1967.4	43.14598	4	2863.402094	2863.446613	K	S	97	122
PSM	NSGMPSGAAAIAVLPVTLDTPMNR	12	sp|P11348|DHPR_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	24	1-UNIMOD:730,4-UNIMOD:35,22-UNIMOD:35	ms_run[1]:scan=1.1.2151.3	51.64627	3	2718.281471	2718.398741	K	K	165	189
PSM	FIPLSEPAPVPPIPNEQQLAR	13	sp|Q6P4Z9|CSN8_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	23	1-UNIMOD:730	ms_run[1]:scan=1.1.2425.2	60.78637	3	2616.349871	2616.458228	R	L	179	200
PSM	VSPSPVDPIVPVVPIGPPPAGFR	14	sp|P52873|PYC_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22	1-UNIMOD:730	ms_run[1]:scan=1.1.2663.2	65.5175	3	2597.380571	2597.488800	K	D	520	543
PSM	GIVNGAAPELPVPTGGPMAGAR	15	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21	1-UNIMOD:730	ms_run[1]:scan=1.1.2022.3	47.2928	3	2336.185571	2335.262505	R	E	8	30
PSM	LLDSLEPPGEPGPSTSIPENDTVDGR	16	tr|Q6AYR1|Q6AYR1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21	1-UNIMOD:730	ms_run[1]:scan=1.1.2238.2	55.36305	3	2995.396271	2995.492895	R	E	119	145
PSM	GVVPLAGTNGETTTQGLDGLSER	17	sp|P05065|ALDOA_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1872.4	35.299	4	2576.288894	2575.339629	K	C	112	135
PSM	GIVNGAAPELPVPTGGPMAGAR	18	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:730,18-UNIMOD:35	ms_run[1]:scan=1.1.1934.2	40.16053	3	2352.159371	2351.257420	R	E	8	30
PSM	TVTNAVVTVPAYFNDSQR	19	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2010.3	46.65287	3	2286.116171	2285.195865	K	Q	138	156
PSM	TVTNAVVTVPAYFNDSQR	20	tr|F1LZI1|F1LZI1_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2020.2	47.06422	3	2286.110471	2285.195865	K	Q	138	156
PSM	ETVSEESNVLCLSK	21	sp|P05197|EF2_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730,11-UNIMOD:39,14-UNIMOD:730	ms_run[1]:scan=1.1.1913.2	38.64337	3	2193.034571	2191.132580	R	S	581	595
PSM	ALANSLACQGK	22	sp|P05065|ALDOA_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730,8-UNIMOD:39,11-UNIMOD:730	ms_run[1]:scan=1.1.1769.2	26.33263	3	1730.895971	1728.947620	R	Y	332	343
PSM	VSPSPVDPIVPVVPIGPPPAGFR	23	sp|P52873|PYC_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730	ms_run[1]:scan=1.1.2690.2	65.92852	4	2597.402094	2597.488800	K	D	520	543
PSM	GIVNGAAPELPVPTGGPMAGAR	24	sp|P62815|VATB2_RAT	0	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1001476, X!Tandem, ]	14	1-UNIMOD:730,18-UNIMOD:35	ms_run[1]:scan=1.1.1935.3	40.27472	4	2352.2012	2351.2572	R	E	8	30
PSM	IDIAGR	25	sp|P85515|ACTZ_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1743.4	24.1372	2	947.538447	947.570684	R	D	183	189
PSM	GLGGAEPDR	26	tr|D4A4P8|D4A4P8_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1880.4	35.89433	2	1176.577647	1174.624905	R	V	4	13