MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data110815_PC12_F3_2000V_120min_13.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	tr|F1LU69|F1LU69_RAT	Ubiquitin-like domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Rps27a-ps28 PE=4 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19.0	null	64-UNIMOD:730	0.07	19.0	1	1	1
PRT	tr|M0R757|M0R757_RAT	Elongation factor 1-alpha OS=Rattus norvegicus (Rat) OX=10116 GN=LOC100360413 PE=3 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	85-UNIMOD:730	0.03	17.0	1	1	1
PRT	sp|P11980|KPYM_RAT	Pyruvate kinase PKM OS=Rattus norvegicus (Rat) OX=10116 GN=Pkm PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16.0	null	279-UNIMOD:730,291-UNIMOD:35	0.03	16.0	1	1	1
PRT	sp|O35314|SCG1_RAT	Secretogranin-1 OS=Rattus norvegicus (Rat) OX=10116 GN=Chgb PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16.0	null	324-UNIMOD:730	0.01	16.0	1	1	1
PRT	tr|D4A517|D4A517_RAT	Solute carrier family 39 (Zinc transporter), member 10 (Predicted), isoform CRA_a OS=Rattus norvegicus (Rat) OX=10116 GN=Slc39a10 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16.0	null	642-UNIMOD:730	0.01	16.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	ESTLHLVLR	1	tr|F1LU69|F1LU69_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	19	1-UNIMOD:730	ms_run[1]:scan=1.1.2153.4	32.84742	3	1370.780471	1370.818854	K	L	64	73
PSM	YYVTIIDAPGHR	2	tr|M0R757|M0R757_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2245.3	36.36122	3	1707.876371	1707.925110	K	D	85	97
PSM	RFDEILEASDGIMVAR	3	sp|P11980|KPYM_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730,13-UNIMOD:35	ms_run[1]:scan=1.1.2971.2	47.86135	3	2141.046971	2141.109359	R	G	279	295
PSM	GHHLAHYR	4	sp|O35314|SCG1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730	ms_run[1]:scan=1.1.1594.2	14.94652	3	1293.664571	1293.699742	R	A	324	332
PSM	HGHQWHHR	5	tr|D4A517|D4A517_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730	ms_run[1]:scan=1.1.1363.2	12.3826	3	1397.675771	1397.712038	K	H	642	650