MTD	mzTab-version	1.0
MTD	mzTab-mode	Complete
MTD	mzTab-type	Identification
MTD	description	JPST000067 -- main
MTD	ms_run[1]-location	D:\JobRequest\ResultFiles\20200410\20200410165636316375^127.0.0.1^jpost@jpost.jpost\Psearch.ProteinPilotExecV5\Data110815_PC12_F4_2000V_120min_10.pilotFin.txt
MTD	software[1]	[MS, MS:1000663, ProteinPilot, 5.0.0.0, 4767]
MTD	software[1]-setting	FASTA=uniprot_rat_20200406.fasta
MTD	software[1]-setting	PARAGON_VERSION=5.0.0.0, 4767
MTD	software[1]-setting	UI_SAMPLE_TYPE=iTRAQ 8plex (Peptide Labeled)
MTD	software[1]-setting	UI_CYS_ALKYLATION=MMTS
MTD	software[1]-setting	UI_DIGESTION=Trypsin
MTD	software[1]-setting	UI_INSTRUMENT=QSTAR Elite ESI
MTD	software[1]-setting	UI_SEARCH_EFFORT=Thorough
MTD	software[1]-setting	UI_ID_FOCUS=Biological modifications
MTD	software[1]-setting	UI_QUANT_TYPE=iTRAQ8PLEX
MTD	software[1]-setting	UI_MIN_UNUSED_PROTSCORE=0.0458
MTD	software[1]-setting	MSTOLERANCE=0.2
MTD	software[1]-setting	MSTOLERANCE_U=Daltons
MTD	software[1]-setting	MSMSTOLERANCE=0.2
MTD	software[1]-setting	MSMSTOLERANCE_U=Daltons
MTD	software[2]	[MS, MS:1001476, X!Tandem, 2015.04.01.1]
MTD	software[2]-setting	DB=userFasta.uniprot_rat_20200406
MTD	software[2]-setting	CLE=[RK]|{P}
MTD	software[2]-setting	MODS=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[2]-setting	IT_MODS=Oxidation (M)
MTD	software[2]-setting	TOL(-)=50
MTD	software[2]-setting	TOL(+)=50
MTD	software[2]-setting	TOLU=ppm
MTD	software[2]-setting	ITOL=250
MTD	software[2]-setting	ITOLU=ppm
MTD	software[2]-setting	PEP_ISOTOPE_ERROR=yes
MTD	software[2]-setting	PFA=1
MTD	software[3]	[MS, MS:1002251, Comet, 2015.02 rev. 0]
MTD	software[3]-setting	Taxon=userFasta.uniprot_rat_20200406
MTD	software[3]-setting	search_enzyme_number=1
MTD	software[3]-setting	FixMod=iTRAQ8plex (K),iTRAQ8plex (N-term),Methylthio (C)
MTD	software[3]-setting	VarMod=Oxidation (M)
MTD	software[3]-setting	max_variable_mods_in_peptide=5
MTD	software[3]-setting	allowed_missed_cleavage=1
MTD	software[3]-setting	peptide_mass_tolerance=50
MTD	software[3]-setting	peptide_mass_units=2
MTD	software[3]-setting	fragment_bin_tol=1.0005
MTD	software[3]-setting	fragment_bin_offset=0.4
MTD	fixed_mod[1]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[1]-site	K
MTD	fixed_mod[1]-position	Anywhere
MTD	fixed_mod[2]	[UNIMOD, UNIMOD:730, iTRAQ8plex,]
MTD	fixed_mod[2]-site	N-term
MTD	fixed_mod[2]-position	Any N-term
MTD	fixed_mod[3]	[UNIMOD, UNIMOD:39, Methylthio,]
MTD	fixed_mod[3]-site	C
MTD	fixed_mod[3]-position	Anywhere
MTD	variable_mod[1]	[UNIMOD, UNIMOD:35, Oxidation,]
MTD	variable_mod[1]-site	M
MTD	variable_mod[1]-position	Anywhere
MTD	protein_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]
MTD	psm_search_engine_score[1]	[http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore]

PRH	accession	description	taxid	species	database	database_version	search_engine	best_search_engine_score[1]	ambiguity_members	modifications	protein_coverage	search_engine_score[1]_ms_run[1]	num_psms_ms_run[1]	num_peptides_distinct_ms_run[1]	num_peptides_unique_ms_run[1]
PRT	sp|P11980|KPYM_RAT	Pyruvate kinase PKM OS=Rattus norvegicus (Rat) OX=10116 GN=Pkm PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22.0	null	279-UNIMOD:730,291-UNIMOD:35	0.03	22.0	1	1	1
PRT	tr|A0A0G2K7W4|A0A0G2K7W4_RAT	Mitogen-activated protein kinase kinase kinase kinase 4 OS=Rattus norvegicus (Rat) OX=10116 GN=Map4k4 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22.0	null	140-UNIMOD:730,618-UNIMOD:730	0.02	22.0	2	2	2
PRT	sp|Q4V887|S39A6_RAT	Zinc transporter ZIP6 OS=Rattus norvegicus (Rat) OX=10116 GN=Slc39a6 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21.0	null	91-UNIMOD:730	0.01	21.0	1	1	1
PRT	tr|M0R757|M0R757_RAT	Elongation factor 1-alpha OS=Rattus norvegicus (Rat) OX=10116 GN=LOC100360413 PE=3 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20.0	null	85-UNIMOD:730,6-UNIMOD:730,20-UNIMOD:730	0.06	20.0	2	2	2
PRT	tr|A0A0G2K8K0|A0A0G2K8K0_RAT	Splicing factor proline and glutamine-rich OS=Rattus norvegicus (Rat) OX=10116 GN=Sfpq PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20.0	null	235-UNIMOD:730	0.03	20.0	1	1	1
PRT	tr|A0A0G2JZ53|A0A0G2JZ53_RAT	Baculoviral IAP repeat-containing 6 OS=Rattus norvegicus (Rat) OX=10116 GN=Birc6 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	1059-UNIMOD:730	0.01	18.0	1	1	1
PRT	sp|P20156|VGF_RAT	Neurosecretory protein VGF OS=Rattus norvegicus (Rat) OX=10116 GN=Vgf PE=1 SV=3	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18.0	null	567-UNIMOD:730,566-UNIMOD:730	0.02	18.0	2	2	2
PRT	sp|O35314|SCG1_RAT	Secretogranin-1 OS=Rattus norvegicus (Rat) OX=10116 GN=Chgb PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	324-UNIMOD:730	0.01	17.0	1	1	1
PRT	sp|P62963|PROF1_RAT	Profilin-1 OS=Rattus norvegicus (Rat) OX=10116 GN=Pfn1 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	128-UNIMOD:730,128-UNIMOD:39,131-UNIMOD:35	0.07	17.0	1	1	1
PRT	tr|F1LQJ7|F1LQJ7_RAT	Phosphoenolpyruvate carboxykinase 2 (mitochondrial) OS=Rattus norvegicus (Rat) OX=10116 GN=Pck2 PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17.0	null	530-UNIMOD:730	0.01	17.0	1	1	1
PRT	sp|Q5PQM0|TM168_RAT	Transmembrane protein 168 OS=Rattus norvegicus (Rat) OX=10116 GN=Tmem168 PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16.0	null	638-UNIMOD:730,640-UNIMOD:35	0.01	16.0	1	1	1
PRT	tr|G3V7U3|G3V7U3_RAT	Protein Wnt OS=Rattus norvegicus (Rat) OX=10116 GN=Wnt2b PE=3 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	174-UNIMOD:730	0.02	15.0	1	1	1
PRT	tr|D4A8A0|D4A8A0_RAT	Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase OS=Rattus norvegicus (Rat) OX=10116 GN=Cad PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15.0	null	832-UNIMOD:730,834-UNIMOD:39	0.00	15.0	1	1	1
PRT	sp|P60711|ACTB_RAT	Actin, cytoplasmic 1 OS=Rattus norvegicus (Rat) OX=10116 GN=Actb PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	316-UNIMOD:730,326-UNIMOD:730	0.03	14.0	1	1	1
PRT	tr|A0A0G2K602|A0A0G2K602_RAT	CP-type G domain-containing protein OS=Rattus norvegicus (Rat) OX=10116 GN=Noa1 PE=4 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	301-UNIMOD:730	0.01	14.0	1	1	1
PRT	sp|P34058|HS90B_RAT	Heat shock protein HSP 90-beta OS=Rattus norvegicus (Rat) OX=10116 GN=Hsp90ab1 PE=1 SV=4	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	181-UNIMOD:730,186-UNIMOD:730	0.01	14.0	1	1	1
PRT	tr|A0A0G2K2E4|A0A0G2K2E4_RAT	Peptidyl-prolyl cis-trans isomerase OS=Rattus norvegicus (Rat) OX=10116 GN=LOC100911252 PE=3 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	83-UNIMOD:730,91-UNIMOD:730	0.06	14.0	1	1	1
PRT	tr|B4F7C2|B4F7C2_RAT	Tubulin beta chain OS=Rattus norvegicus (Rat) OX=10116 GN=Tubb4a PE=1 SV=1	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	253-UNIMOD:730,257-UNIMOD:35	0.02	14.0	1	1	1
PRT	sp|P05065|ALDOA_RAT	Fructose-bisphosphate aldolase A OS=Rattus norvegicus (Rat) OX=10116 GN=Aldoa PE=1 SV=2	null	null	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14.0	null	244-UNIMOD:730,251-UNIMOD:35	0.04	14.0	1	1	1

PSH	sequence	PSM_ID	accession	unique	database	database_version	search_engine	search_engine_score[1]	modifications	spectra_ref	retention_time	charge	exp_mass_to_charge	calc_mass_to_charge	pre	post	start	end
PSM	RFDEILEASDGIMVAR	1	sp|P11980|KPYM_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22	1-UNIMOD:730,13-UNIMOD:35	ms_run[1]:scan=1.1.2122.2	47.13738	3	2141.052071	2141.109359	R	G	279	295
PSM	GLAHLHIHHVIHR	2	tr|A0A0G2K7W4|A0A0G2K7W4_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	22	1-UNIMOD:730	ms_run[1]:scan=1.1.1488.2	19.5294	3	1843.085171	1843.074843	R	D	140	153
PSM	VHIHHDHER	3	sp|Q4V887|S39A6_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	21	1-UNIMOD:730	ms_run[1]:scan=1.1.1417.3	13.9034	3	1482.735371	1482.774697	R	H	91	100
PSM	YYVTIIDAPGHR	4	tr|M0R757|M0R757_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1746.3	35.7825	3	1707.870971	1707.925110	K	D	85	97
PSM	QHHAPYHQQHHQGPPPGGPGPR	5	tr|A0A0G2K8K0|A0A0G2K8K0_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	20	1-UNIMOD:730	ms_run[1]:scan=1.1.1431.3	14.91502	4	2720.305694	2720.348786	R	T	235	257
PSM	THINIVVIGHVDSGK	6	tr|M0R757|M0R757_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730,15-UNIMOD:730	ms_run[1]:scan=1.1.1656.3	29.96112	4	2196.243294	2196.284010	K	S	6	21
PSM	RHPQHLHQQHHGDAAQHTR	7	tr|A0A0G2JZ53|A0A0G2JZ53_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730	ms_run[1]:scan=1.1.1257.2	11.70507	4	2594.253294	2594.313069	R	T	1059	1078
PSM	HFHHALPPAR	8	sp|P20156|VGF_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	18	1-UNIMOD:730	ms_run[1]:scan=1.1.1449.3	16.66487	3	1485.793871	1485.826005	R	H	567	577
PSM	GHHLAHYR	9	sp|O35314|SCG1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.1430.2	14.76565	3	1293.668471	1293.699742	R	A	324	332
PSM	CYEMASHLR	10	sp|P62963|PROF1_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730,1-UNIMOD:39,4-UNIMOD:35	ms_run[1]:scan=1.1.1546.2	22.47987	3	1474.638371	1474.667381	K	R	128	137
PSM	IFHVNWFR	11	tr|F1LQJ7|F1LQJ7_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	17	1-UNIMOD:730	ms_run[1]:scan=1.1.2390.2	51.08563	3	1421.751071	1421.787494	R	R	530	538
PSM	HWMLHFPR	12	sp|Q5PQM0|TM168_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	16	1-UNIMOD:730,3-UNIMOD:35	ms_run[1]:scan=1.1.1644.3	29.0441	3	1442.707571	1442.754814	K	I	638	646
PSM	RHFHHALPPAR	13	sp|P20156|VGF_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.1445.3	16.28388	4	1641.887294	1641.927116	R	H	566	577
PSM	GRHHDQR	14	tr|G3V7U3|G3V7U3_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730	ms_run[1]:scan=1.1.1448.2	16.53183	3	1208.650271	1208.642955	R	G	174	181
PSM	IDCWFLHR	15	tr|D4A8A0|D4A8A0_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	15	1-UNIMOD:730,3-UNIMOD:39	ms_run[1]:scan=1.1.2787.2	55.97087	3	1437.687371	1438.715651	R	M	832	840
PSM	FAHHHLR	16	tr|A0A0G2K7W4|A0A0G2K7W4_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1447.2	16.445	3	1220.650271	1220.683363	K	S	618	625
PSM	EITALAPSTMK	17	sp|P60711|ACTB_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,11-UNIMOD:730	ms_run[1]:scan=1.1.1739.4	35.23969	3	1768.980371	1769.021830	K	I	316	327
PSM	NQNPSSRSR	18	tr|A0A0G2K602|A0A0G2K602_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730	ms_run[1]:scan=1.1.1534.4	21.50635	2	1348.699047	1348.711428	K	T	301	310
PSM	VILHLK	19	sp|P34058|HS90B_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,6-UNIMOD:730	ms_run[1]:scan=1.1.1665.3	30.57875	3	1329.857771	1329.895766	K	E	181	187
PSM	FEDENFILK	20	tr|A0A0G2K2E4|A0A0G2K2E4_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,9-UNIMOD:730	ms_run[1]:scan=1.1.2139.2	47.58707	3	1761.945371	1761.976260	K	H	83	92
PSM	LAVNMVPFPR	21	tr|B4F7C2|B4F7C2_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,5-UNIMOD:35	ms_run[1]:scan=1.1.1841.4	40.58613	3	1461.791771	1462.827310	K	L	253	263
PSM	FSNEEIAMATVTALR	22	sp|P05065|ALDOA_RAT	1	userFasta.uniprot_rat_20200406	userFasta.uniprot_rat_20200406	[MS, MS:1002251, Comet, ]	14	1-UNIMOD:730,8-UNIMOD:35	ms_run[1]:scan=1.1.2278.2	49.75692	3	1971.955871	1972.024232	K	R	244	259