Raw file Experiment Fraction Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS submitted MS/MS submitted (SIL) MS/MS submitted (ISO) MS/MS submitted (PEAK) MS/MS identified MS/MS identified (SIL) MS/MS identified (ISO) MS/MS identified (PEAK) MS/MS identified [%] MS/MS identified (SIL) [%] MS/MS identified (ISO) [%] MS/MS identified (PEAK) [%] Peptide sequences identified Peaks Peaks sequenced Peaks sequenced [%] Peaks repeatedly sequenced Peaks repeatedly sequenced [%] Isotope patterns Isotope patterns sequenced Isotope patterns sequenced (z>1) Isotope patterns sequenced [%] Isotope patterns sequenced (z>1) [%] Isotope patterns repeatedly sequenced Isotope patterns repeatedly sequenced [%] Recalibrated Av. absolute mass deviation [ppm] Mass standard deviation [ppm] Av. absolute mass deviation [mDa] Mass standard deviation [mDa] 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R2_-75 Rep2 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 2071 16308 0 19379 13237 0 6142 559 537 0 22 2.9 4.1 0 0.36 478 253565 13889 5.5 869 6.3 43192 11224 11121 26 27 1035 9.2 + 0.40819 0.52536 153640 160940 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R3_-40 Rep3 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1714 12762 0 14927 10597 0 4330 1696 1624 0 72 11 15 0 1.7 1132 248689 11507 4.6 468 4.1 46348 9640 9412 21 25 675 7 + 0.7917 1.0396 151800 161830 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R2_-60 Rep2 102 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1528 21994 0 25271 18717 0 6554 5328 5111 0 217 21 27 0 3.3 3803 302691 19060 6.3 1455 7.6 60239 15666 15458 26 27 1920 12 + 0.50043 0.7311 168370 177720 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_input_fr1 Rep1 301 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 12597 36525 0 49051 23999 0 25052 1958 1771 0 187 4 7.4 0 0.75 1127 1017083 29306 2.9 3057 10 89673 16559 14716 18 29 4337 26 + 0.94475 1.2835 105320 120620 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R4_-45 Rep4 101 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1470 19870 0 22330 17410 0 4920 5039 4898 0 141 23 28 0 2.9 3720 362911 18010 5 1004 5.6 75974 15568 15315 20 22 1393 8.9 + 0.57493 0.82857 172060 182920 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R1_-55 Rep1 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1657 20870 0 25095 16645 0 8450 2645 2482 0 163 11 15 0 1.9 1869 353119 18942 5.4 782 4.1 67291 15131 14809 22 24 1121 7.4 + 0.79177 1.0514 157640 168000 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_input_fr3 Rep2 303 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 8183 57415 0 73337 41493 0 31844 11970 11122 0 848 16 27 0 2.7 9158 851238 48583 5.7 3453 7.1 116400 31046 30107 27 30 7209 23 + 0.48669 0.7301 173470 182310 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_IC_fr1 Rep2 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 19075 11103 0 17508 4698 0 12810 652 603 0 49 3.7 13 0 0.38 452 1136452 7522 0.66 267 3.5 62879 4185 3782 6.7 17 454 11 + 0.53967 0.72348 138800 146420 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R4_-40 Rep4 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 3356 7464 0 8977 5951 0 3026 249 244 0 5 2.8 4.1 0 0.17 219 261790 5934 2.3 663 11 31147 4601 4443 15 28 856 19 + 1.3013 1.6287 147940 159050 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R1_-55 Rep1 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 2066 20227 0 23662 16792 0 6870 1090 1063 0 27 4.6 6.3 0 0.39 866 316404 18111 5.7 865 4.8 57258 15036 14819 26 28 1103 7.3 + 1.1179 1.3665 155300 165320 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_input_fr1 Rep3 301 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 10890 42574 0 57091 28057 0 29034 2248 2056 0 192 3.9 7.3 0 0.66 1255 952005 34357 3.6 3707 11 92432 19038 16814 21 30 5276 28 + 0.85268 1.162 106490 120380 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_CS_fr2 Rep4 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 18601 13302 0 18257 8347 0 9910 160 155 0 5 0.88 1.9 0 0.05 117 929241 11417 1.2 485 4.2 58857 7385 6799 13 23 762 10 + 0.94988 1.2804 131390 142460 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_input_fr1 Rep2 301 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 11915 38742 0 51736 25748 0 25988 2102 1916 0 186 4.1 7.4 0 0.72 1297 979759 31493 3.2 3052 9.7 94123 18098 16253 19 30 4674 26 + 0.93134 1.271 108390 124670 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_CS_fr2 Rep3 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 16618 19353 0 26245 12461 0 13784 1041 969 0 72 4 7.8 0 0.52 631 1089430 16388 1.5 834 5.1 87960 10516 9829 12 18 1428 14 + 1.0979 1.4093 141710 151510 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R1_-60 Rep1 102 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1519 22122 0 25257 18987 0 6270 5677 5406 0 271 22 28 0 4.3 4065 312472 19287 6.2 1376 7.1 62783 15994 15825 25 26 1827 11 + 0.48522 0.71995 168300 177400 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_CS_fr3 Rep3 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 12494 32813 0 41985 23641 0 18344 2346 2224 0 122 5.6 9.4 0 0.67 1901 915737 28820 3.1 1500 5.2 89396 18467 17808 21 27 3743 20 + 0.67772 0.87522 177050 184650 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R3_-55 Rep3 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1714 21058 0 25048 17068 0 7980 2316 2181 0 135 9.2 13 0 1.7 1657 357919 19123 5.3 801 4.2 68023 15551 15251 23 24 1098 7.1 + 0.89951 1.1755 152260 162770 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_input_fr2 Rep3 302 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 6909 64472 0 80934 48010 0 32924 13097 12163 0 934 16 25 0 2.8 9142 770784 54231 7 4673 8.6 110138 33966 32862 31 35 9264 27 + 0.52456 0.78145 163410 172560 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R2_-55 Rep2 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 2071 21025 0 24494 17556 0 6938 748 727 0 21 3.1 4.1 0 0.3 576 312089 18714 6 1057 5.6 55844 15586 15334 28 30 1304 8.4 + 0.96993 1.2255 149800 159650 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R3_-75 Rep3 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 3373 10778 0 13284 8272 0 5012 316 298 0 18 2.4 3.6 0 0.36 216 243697 7531 3.1 1243 17 24830 5298 5200 21 23 1241 23 + 0.62426 0.81516 156350 162560 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R4_-55 Rep4 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 3356 14429 0 17328 11530 0 5798 675 650 0 25 3.9 5.6 0 0.43 457 328591 11160 3.4 1551 14 38461 8440 8140 22 25 1613 19 + 1.1626 1.4605 147450 157720 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R4_-60 Rep4 102 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1470 22407 0 25792 19022 0 6770 5618 5401 0 217 22 28 0 3.2 4058 313496 19710 6.3 1329 6.7 64429 16215 16072 25 26 1800 11 + 0.49513 0.74017 166650 176030 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R4_-75 Rep4 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1606 19831 0 23320 16342 0 6978 2033 1846 0 187 8.7 11 0 2.7 1524 318153 18041 5.7 691 3.8 63498 14777 14573 23 24 966 6.5 + 0.51928 0.72218 158110 166350 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R2_-75 Rep2 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1649 19338 0 22776 15900 0 6876 2009 1856 0 153 8.8 12 0 2.2 1594 307238 17539 5.7 658 3.8 61086 14343 14172 23 24 906 6.3 + 0.54372 0.75025 161970 169930 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R3_-80 Rep3 103 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1494 21098 0 25130 17066 0 8064 4569 4304 0 265 18 25 0 3.3 3632 254748 18341 7.2 1253 6.8 50122 14138 13994 28 29 2028 14 + 0.44541 0.69264 162170 169810 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R4_-80 Rep4 103 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1469 21129 0 25306 16952 0 8354 3448 3326 0 122 14 20 0 1.5 2673 244980 17924 7.3 1488 8.3 47733 13507 13328 28 29 2254 17 + 0.43196 0.66004 167290 174870 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R1_-75 Rep1 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1656 19591 0 23140 16042 0 7098 2326 2150 0 176 10 13 0 2.5 1785 316542 17803 5.6 685 3.8 61812 14447 14266 23 24 1017 7 + 0.53508 0.74587 160680 168630 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_IC_fr2 Rep3 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 17143 18157 0 26176 10138 0 16038 553 504 0 49 2.1 5 0 0.31 312 1212793 13340 1.1 1035 7.8 80738 7909 7127 9.8 15 1151 15 + 0.80284 1.0331 152570 160280 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_IC_fr2 Rep1 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 19329 9511 0 14161 4861 0 9300 97 95 0 2 0.68 2 0 0.022 76 1011923 6783 0.67 512 7.5 59224 3939 3505 6.7 14 559 14 + 0.67334 0.85287 146640 157140 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R1_-40 Rep1 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 2066 10362 0 12380 8344 0 4036 293 288 0 5 2.4 3.5 0 0.12 271 212726 9247 4.3 284 3.1 34737 7794 7595 22 34 455 5.8 + 0.939 1.1957 145300 156120 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R3_-40 Rep3 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 3373 6756 0 8302 5210 0 3092 134 127 0 7 1.6 2.4 0 0.23 101 246677 5274 2.1 557 11 26834 4119 3959 15 32 674 16 + 1.4569 1.7228 141610 150160 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_IC_fr2 Rep4 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 18697 12296 0 18693 5899 0 12794 368 342 0 26 2 5.8 0 0.2 270 1124954 8230 0.73 666 8.1 66573 4512 4022 6.8 12 646 14 + 0.59961 0.82189 147220 153560 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_input_fr2 Rep1 302 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 17774 16155 0 22863 9447 0 13416 817 736 0 81 3.6 7.8 0 0.6 489 1032858 13497 1.3 852 6.3 80770 7514 6395 9.3 19 1322 18 + 1.1291 1.4799 135870 149160 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_CS_fr3 Rep2 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 10250 40080 0 51274 28886 0 22388 3633 3435 0 198 7.1 12 0 0.88 3024 829772 34748 4.2 1950 5.6 94973 22525 21738 24 29 4713 21 + 0.82507 1.0615 173070 181050 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R2_-45 Rep2 101 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1528 19323 0 21875 16771 0 5104 4781 4649 0 132 22 28 0 2.6 3498 348472 17249 4.9 1083 6.3 71043 14766 14494 21 23 1476 10 + 0.56518 0.81038 172000 182270 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R2_-40 Rep2 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1650 13635 0 15948 11322 0 4626 1649 1577 0 72 10 14 0 1.6 1136 264595 12338 4.7 455 3.7 50194 10406 10139 21 25 639 6.1 + 0.86707 1.1185 157190 167200 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R3_-55 Rep3 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 3373 14621 0 17678 11564 0 6114 494 486 0 8 2.8 4.2 0 0.13 291 320572 11197 3.5 1664 15 36131 8356 8110 23 27 1726 21 + 1.2323 1.4961 151380 160880 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R3_-60 Rep3 102 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1495 22467 0 25897 19037 0 6860 6561 6303 0 258 25 33 0 3.8 4881 335354 20224 6 1158 5.7 68935 16822 16657 24 25 1578 9.4 + 0.47386 0.69613 159440 168980 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_CS_fr1 Rep3 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 18507 13894 0 19107 8681 0 10426 244 226 0 18 1.3 2.6 0 0.17 201 1309562 12509 0.96 249 2 78312 7886 7174 10 22 664 8.4 + 1.0462 1.3412 125480 135720 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R2_-40 Rep2 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 2072 10140 0 12235 8045 0 4190 210 207 0 3 1.7 2.6 0 0.072 192 225785 8964 4 346 3.9 34708 7370 7152 21 35 509 6.9 + 0.95674 1.237 146380 158600 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_IC_fr3 Rep2 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 8656 49549 0 63710 35388 0 28322 6982 6366 0 616 11 18 0 2.2 4714 934075 42343 4.5 2425 5.7 117265 27240 26338 23 26 5787 21 + 0.6369 0.89304 162920 171820 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_input_fr3 Rep1 303 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 8155 58955 0 74890 43020 0 31870 12882 11969 0 913 17 28 0 2.9 9886 796650 49560 6.2 3770 7.6 112314 31654 30732 28 32 7784 25 + 0.53454 0.7936 182640 190430 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R4_-75 Rep4 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 3355 10864 0 13248 8480 0 4768 456 426 0 30 3.4 5 0 0.63 351 253340 7798 3.1 1212 16 26860 5633 5539 21 23 1193 21 + 0.63061 0.86063 154630 161890 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R1_-45 Rep1 101 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1519 19030 0 21265 16795 0 4470 5214 5081 0 133 25 30 0 3 3777 346163 17134 4.9 1055 6.2 71427 14799 14557 21 23 1439 9.7 + 0.57778 0.82853 170610 180430 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R2_-55 Rep2 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1650 21150 0 25017 17283 0 7734 2352 2241 0 111 9.4 13 0 1.4 1714 356291 19338 5.4 729 3.8 70247 15906 15628 23 24 982 6.2 + 0.91154 1.1843 157740 168040 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R4_-40 Rep4 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1607 13946 0 16300 11592 0 4708 1751 1680 0 71 11 14 0 1.5 1137 270884 12472 4.6 570 4.6 51613 10427 10172 20 24 782 7.5 + 0.76558 1.0401 157800 167990 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_IC_fr1 Rep1 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 19570 8961 0 13561 4361 0 9200 81 73 0 8 0.6 1.7 0 0.087 73 1256325 6658 0.53 231 3.5 68956 3811 3471 5.5 15 401 11 + 0.68249 0.90712 142080 154710 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_input_fr3 Rep4 303 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 7497 61646 0 77863 45429 0 32434 12680 11693 0 987 16 26 0 3 9763 841554 52099 6.2 3861 7.4 120375 33656 32705 28 32 8125 24 + 0.55469 0.80391 179910 188110 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_IC_fr3 Rep3 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 8658 51325 0 66381 36269 0 30112 6939 6266 0 673 10 17 0 2.2 4526 1013976 43803 4.3 2500 5.7 129900 28025 27053 22 24 5947 21 + 0.63167 0.8866 158540 167840 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_CS_fr1 Rep1 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 17198 18422 0 25842 11002 0 14840 593 559 0 34 2.3 5.1 0 0.23 480 1308031 16112 1.2 354 2.2 82243 9890 9042 12 25 967 9.8 + 0.87976 1.1293 129920 139220 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_CS_fr3 Rep1 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 9695 42066 0 53688 30444 0 23244 4816 4564 0 252 9 15 0 1.1 4011 806853 36373 4.5 2061 5.7 96117 23657 22875 25 30 5012 21 + 0.71241 0.96955 173700 182110 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_IC_fr3 Rep4 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 8632 51399 0 65779 37019 0 28760 6747 6129 0 618 10 17 0 2.1 4592 971506 44270 4.6 2536 5.7 129487 28868 27743 22 25 5921 21 + 0.61547 0.87664 159100 168330 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_CS_fr3 Rep4 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 9473 42508 0 53828 31188 0 22640 4351 4095 0 256 8.1 13 0 1.1 3553 824579 36632 4.4 1950 5.3 100517 24698 23977 25 31 4851 20 + 0.80682 1.0474 160660 169220 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_IC_fr1 Rep3 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 19278 10449 0 16881 4017 0 12864 481 447 0 34 2.8 11 0 0.26 326 1139059 6669 0.59 221 3.3 59571 3619 3201 6.1 16 357 9.9 + 0.4603 0.59747 137880 144370 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R1_-80 Rep1 103 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1519 20645 0 24796 16494 0 8302 3240 3080 0 160 13 19 0 1.9 2530 221528 17113 7.7 1667 9.7 42089 12669 12533 30 31 2326 18 + 0.42157 0.64595 168370 176260 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_IC_fr3 Rep1 403 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 8341 53986 0 69762 38210 0 31552 7642 6878 0 764 11 18 0 2.4 5060 1005802 45986 4.6 2653 5.8 136114 29657 28631 22 24 6135 21 + 0.61692 0.8894 158340 167660 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_CS_R1_-75 Rep1 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 2066 16883 0 20248 13518 0 6730 653 627 0 26 3.2 4.6 0 0.39 607 265433 14347 5.4 789 5.5 45957 11505 11383 25 26 980 8.5 + 0.4773 0.6437 154290 162010 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R3_-75 Rep3 203 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1713 19613 0 22862 16364 0 6498 2012 1843 0 169 8.8 11 0 2.6 1584 319131 17883 5.6 673 3.8 62390 14743 14558 24 24 968 6.6 + 0.53367 0.75216 155680 163820 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_CS_fr2 Rep2 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 17845 15870 0 22135 9605 0 12530 379 352 0 27 1.7 3.7 0 0.22 204 1083754 13374 1.2 801 6 72452 8049 7380 11 19 1068 13 + 0.72392 0.98123 142270 151130 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_input_fr2 Rep4 302 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 16822 20288 0 28339 12237 0 16102 1134 1008 0 126 4 8.2 0 0.78 771 1092834 16394 1.5 1558 9.5 77274 8831 7654 11 22 2071 23 + 0.76478 1.0776 139400 151220 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_IC_fr1 Rep4 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 19067 11288 0 18408 4168 0 14240 501 455 0 46 2.7 11 0 0.32 332 1163886 7003 0.6 264 3.8 61401 3739 3258 6.1 15 376 10 + 0.54395 0.68069 140120 146430 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R1_CS_fr2 Rep1 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 17082 18378 0 25003 11753 0 13250 475 460 0 15 1.9 3.9 0 0.11 384 1008501 16108 1.6 542 3.4 71076 10332 9632 15 26 1147 11 + 0.83115 1.0899 143650 151640 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_CS_fr1 Rep2 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 18591 13663 0 19244 8082 0 11162 301 278 0 23 1.6 3.4 0 0.21 251 1385702 12110 0.87 267 2.2 84071 7326 6652 8.7 20 653 8.9 + 0.95007 1.2266 125610 135900 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R2_-80 Rep2 103 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1527 20530 0 24832 16228 0 8604 3095 2937 0 158 12 18 0 1.8 2360 219617 16914 7.7 1724 10 41133 12178 12049 30 31 2457 20 + 0.41835 0.6377 167560 175430 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R4_-55 Rep4 202 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1607 21235 0 25296 17174 0 8122 2356 2202 0 154 9.3 13 0 1.9 1642 356388 19095 5.4 840 4.4 69800 15563 15236 22 23 1060 6.8 + 0.86494 1.1432 154270 164690 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R3_input_fr3 Rep3 303 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 8108 58497 0 74881 42113 0 32768 12679 11676 0 1003 17 28 0 3.1 9365 851063 49329 5.8 3852 7.8 113179 30650 29720 27 31 7775 25 + 0.49861 0.75217 175510 184280 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_input_fr2 Rep2 302 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 6570 65986 0 83560 48412 0 35148 11971 11129 0 842 14 23 0 2.4 7722 720657 54400 7.5 5420 10 103276 33009 31929 32 36 9879 30 + 0.50877 0.75469 165210 174590 201119_C20_020_OE480_TMT_FAIMS_45-60-80_rev00_20_065_JEi_JE013_input_R3_-45 Rep3 101 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 1495 20066 0 22643 17489 0 5154 6272 6085 0 187 28 35 0 3.6 4771 366903 18446 5 788 4.3 77578 15978 15711 21 22 1203 7.5 + 0.54828 0.77464 166670 178150 201119_C20_020_OE480_TMT_FAIMS_40-55-75_rev00_20_065_JEi_JE013_IC_R1_-40 Rep1 201 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 1657 14741 0 17159 12323 0 4836 2170 2083 0 87 13 17 0 1.8 1421 283095 13293 4.7 600 4.5 54168 11098 10788 20 24 828 7.5 + 0.73553 0.99595 159410 170390 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R2_IC_fr2 Rep2 402 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 18736 12363 0 18003 6723 0 11280 684 652 0 32 3.8 9.7 0 0.28 539 1034210 9508 0.92 533 5.6 64883 5528 5018 8.5 15 639 12 + 0.75054 0.98371 141630 149480 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_CS_fr1 Rep4 401 Trypsin/P Specific Trypsin/P Specific True Acetyl (Protein N-term);Acetyl (K);Oxidation (M) Carbamidomethyl (C) True False 1 4 Reporter ion MS2 19059 11525 0 15768 7282 0 8486 140 134 0 6 0.89 1.8 0 0.071 119 1406258 10389 0.74 197 1.9 82544 6751 6106 8.2 19 456 6.8 + 0.78367 1.0572 118700 130550 201119_C20_020_OE480_TMT_rev00_20_065_JEi_JE013_R4_input_fr1 Rep4 301 Trypsin/P Specific False Acetyl (Protein N-term);Oxidation (M) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 11806 38949 0 52339 25559 0 26780 1887 1767 0 120 3.6 6.9 0 0.45 1158 975246 31565 3.2 3121 9.9 91733 17888 15909 20 30 4603 26 + 0.92966 1.2876 103950 119710 210811_C21_022_OE480_OE480_TMT_Rev01_PTM_20_065_JEI_JE013_p_R1 Rep1 501 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 4567 76444 0 100148 52740 0 47408 10648 10314 0 334 11 20 0 0.7 5274 615578 61025 9.9 5400 8.8 83401 35454 34778 43 46 10739 30 + 0.773 1.0644 153510 165490 210811_C21_022_OE480_OE480_TMT_Rev01_PTM_20_065_JEI_JE013_p_R2 Rep2 501 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 4853 77590 0 102128 53052 0 49076 9604 9298 0 306 9.4 18 0 0.62 4829 636559 61952 9.7 5316 8.6 84490 36058 35298 43 46 10641 30 + 0.88656 1.1815 150690 162530 210811_C21_022_OE480_OE480_TMT_Rev01_PTM_20_065_JEI_JE013_p_R3 Rep3 501 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 4867 77655 0 101982 53328 0 48654 10742 10433 0 309 11 20 0 0.64 5348 653293 62196 9.5 5222 8.4 87979 36600 35903 42 45 10536 29 + 0.75261 1.0243 150850 163270 210811_C21_022_OE480_OE480_TMT_Rev01_PTM_20_065_JEI_JE013_p_R4 Rep4 501 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) True False 1 2 Reporter ion MS2 4862 77651 0 101810 53492 0 48318 8865 8608 0 257 8.7 16 0 0.53 4597 659432 62541 9.5 5134 8.2 88870 36911 36213 42 44 10566 29 + 0.87762 1.1821 148070 160270 Total 587481 2080113 0 2652848 1507378 0 1145470 265524 249946 0 15578 10 17 0 1.4 37283 49971022 5497850 1180740 1137941 21 27 214070 18 0.63116 0.90482 162370 172490