Raw file Experiment Fraction Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param 240_LF_45_63-1 45-63_1 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8270 90384 0 105593 75175 0 30418 42372 41065 0 1307 40 55 0 4.3 22242 830694 71899 8.7 10154 14 106974 53384 52043 50 55 13012 24 + 0.39778 0.60365 0.27538 0.44071 240_LF_45_63-2 45-63_2 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8165 91016 0 105700 76332 0 29368 42096 40961 0 1135 40 54 0 3.9 22823 857886 74700 8.7 9336 12 111908 56189 54924 50 55 12571 22 + 0.40146 0.61144 0.2751 0.44422 240_LF_45_63-3 45-63_3 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8198 90053 0 106812 73294 0 33518 40200 39063 0 1137 38 53 0 3.4 22168 827763 74002 8.9 9268 13 105369 54237 52684 51 57 12160 22 + 0.38766 0.59513 0.26862 0.44036 240_LF_45_63-4 45-63_4 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8238 90247 0 105088 75406 0 29682 41769 40570 0 1199 40 54 0 4 23231 845382 74638 8.8 9244 12 111140 56161 54798 51 55 12486 22 + 0.39846 0.60425 0.27336 0.44266 240_LF_45-1 -45_1 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8469 89581 0 103931 75231 0 28700 42427 41107 0 1320 41 55 0 4.6 22687 829763 72493 8.7 9815 14 109742 54516 53192 50 56 12755 23 + 0.38612 0.59031 0.26941 0.43895 240_LF_45-2 -45_2 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8192 90276 0 105763 74789 0 30974 41669 40372 0 1297 39 54 0 4.2 22487 841969 72722 8.6 9976 14 107673 53960 52525 50 55 12954 24 + 0.39765 0.61299 0.27708 0.45561 240_LF_45-3 -45_3 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8385 89450 0 104621 74279 0 30342 41890 40810 0 1080 40 55 0 3.6 22514 820282 71711 8.7 10142 14 105252 53173 51792 51 56 13215 25 + 0.39429 0.59624 0.27011 0.43301 240_LF_45-4 -45_4 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8234 90758 0 105391 76125 0 29266 42896 41582 0 1314 41 55 0 4.5 23141 843220 72973 8.7 10046 14 109858 54810 53485 50 55 13029 24 + 0.39389 0.59204 0.27283 0.43581 240_LF_64_74-1 64-74_1 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8210 90404 0 106087 74721 0 31366 41671 40534 0 1137 39 54 0 3.6 22056 844930 73105 8.7 9903 14 107948 54131 52767 50 56 12877 24 + 0.38823 0.59192 0.26864 0.43674 240_LF_64_74-2 64-74_2 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8081 91082 0 106885 75279 0 31606 41819 40603 0 1216 39 54 0 3.8 22861 855141 74945 8.8 9320 12 110999 55878 54501 50 56 12533 22 + 0.39931 0.60849 0.27202 0.43638 240_LF_64_74-3 64-74_3 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8265 89962 0 105201 74723 0 30478 41257 40077 0 1180 39 54 0 3.9 22452 845539 73856 8.7 9333 13 108806 54956 53584 51 56 12539 23 + 0.39201 0.60014 0.26947 0.43898 240_LF_64_74-4 64-74_4 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8209 90149 0 106205 74093 0 32112 39607 38403 0 1204 37 52 0 3.7 21458 840715 73571 8.8 9349 13 108698 54245 52797 50 56 12381 23 + 0.39448 0.59832 0.27131 0.43779 240_LF_75-1 +75_1 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8276 89958 0 104142 75774 0 28368 43582 42396 0 1186 42 56 0 4.2 23687 859449 72770 8.5 10142 14 109633 54496 53270 50 55 13464 25 + 0.39739 0.60167 0.269 0.43251 240_LF_75-2 +75_2 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8234 90190 0 105136 75244 0 29892 42574 41412 0 1162 40 55 0 3.9 23396 857484 73809 8.6 9262 13 111004 55377 54050 50 55 12428 22 + 0.3934 0.60389 0.2695 0.44119 240_LF_75-3 +75_3 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8235 89895 0 105329 74461 0 30868 41095 39915 0 1180 39 54 0 3.8 22255 836107 72379 8.7 10180 14 104435 53100 51785 51 56 13420 25 + 0.39374 0.59686 0.26524 0.42433 240_LF_75-4 +75_4 2 Trypsin/P Specific False Oxidation (M);Acetyl (N-term);Carbamidomethyl (C);Deamidation (NQ);Gln->pyro-Glu;Glu->pyro-Glu Carbamidomethyl (C) Oxidation (M) True False 1 2 Standard 8223 89821 0 105292 74350 0 30942 41194 39991 0 1203 39 54 0 3.9 23701 830608 73001 8.8 9783 13 104589 53549 52436 51 56 13182 25 + 0.40451 0.61396 0.2697 0.43406 240_Phospho_45_63-1 45-63_1 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7820 91241 0 116975 65507 0 51468 22651 21733 0 918 19 33 0 1.8 8903 746989 70247 9.4 9724 14 77671 41600 40749 54 59 13898 33 + 0.39112 0.60138 0.33464 0.55684 240_Phospho_45_63-2 45-63_2 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7925 90700 0 116355 65045 0 51310 21249 20502 0 747 18 32 0 1.5 7589 761495 68573 9 10345 15 76904 40560 39608 53 58 14190 35 + 0.38817 0.59498 0.33376 0.55336 240_Phospho_45_63-3 45-63_3 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7955 90391 0 114586 66196 0 48390 25404 24460 0 944 22 37 0 2 10818 783700 70255 9 10098 14 80190 43343 42170 54 60 14167 33 + 0.36318 0.55592 0.29622 0.49628 240_Phospho_45_63-4 45-63_4 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8036 90176 0 115966 64386 0 51580 22070 21286 0 784 19 33 0 1.5 8010 749170 67252 9 10650 16 75172 39997 38944 53 59 14060 35 + 0.39246 0.60373 0.33563 0.55754 240_Phospho_45-1 -45_1 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8496 88258 0 109883 66633 0 43250 24047 23186 0 861 22 35 0 2 9809 755248 68125 9 9988 15 81338 42831 41843 53 58 13905 32 + 0.36353 0.56453 0.30013 0.51019 240_Phospho_45-2 -45_2 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7898 90856 0 114464 67248 0 47216 26331 25370 0 961 23 38 0 2 10907 762390 69556 9.1 10167 15 82018 43563 42555 53 59 14045 32 + 0.36659 0.56668 0.30227 0.50461 240_Phospho_45-3 -45_3 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8101 89754 0 115103 64405 0 50698 23456 22630 0 826 20 35 0 1.6 9461 745148 67724 9.1 10390 15 76918 40913 39892 53 59 13721 34 + 0.38046 0.58466 0.31599 0.52792 240_Phospho_45-4 -45_4 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8056 89937 0 115963 63911 0 52052 21683 20846 0 837 19 33 0 1.6 8305 777656 68354 8.8 10396 15 74969 40094 39155 53 59 14045 35 + 0.38217 0.58442 0.32074 0.53001 240_Phospho_64_74-1 64-74_1 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7617 91942 0 118520 65364 0 53156 22195 21338 0 857 19 33 0 1.6 8341 742208 69543 9.4 10319 15 76151 41356 40360 54 60 14123 34 + 0.3793 0.58921 0.31927 0.53446 240_Phospho_64_74-2 64-74_2 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7800 91174 0 117190 65158 0 52032 23746 22865 0 881 20 35 0 1.7 9245 756014 69086 9.1 10089 15 75938 41284 40386 54 59 14022 34 + 0.37141 0.5711 0.31431 0.52033 240_Phospho_64_74-3 64-74_3 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8118 89677 0 113677 65677 0 48000 22615 21739 0 876 20 33 0 1.8 8453 758004 67794 8.9 10441 15 76471 40699 39736 53 59 14294 35 + 0.37026 0.56768 0.31257 0.51796 240_Phospho_64_74-4 64-74_4 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8118 89733 0 116090 63376 0 52714 19161 18375 0 786 17 29 0 1.5 6642 750632 67252 9 10483 16 74474 38597 37641 52 57 14067 36 + 0.37693 0.58112 0.32406 0.53756 240_Phospho_75-1 +75_1 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 8056 90098 0 114068 66128 0 47940 22963 22104 0 859 20 33 0 1.8 8868 759494 68628 9 10488 15 77778 41937 40917 54 59 14103 34 + 0.39928 0.61629 0.34077 0.57467 240_Phospho_75-2 +75_2 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7805 91113 0 115184 67042 0 48142 26020 25074 0 946 23 37 0 2 10841 766884 69950 9.1 10191 15 80917 43195 42189 53 59 14195 33 + 0.37522 0.58056 0.30712 0.51794 240_Phospho_75-3 +75_3 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7124 94041 0 118066 70016 0 48050 30414 29479 0 935 26 42 0 1.9 14478 779577 71963 9.2 10392 14 86870 46162 45433 53 57 13657 30 + 0.37306 0.57053 0.28749 0.47796 240_Phospho_75-4 +75_4 1 Trypsin/P Specific False Oxidation (M);Phospho (STY);Acetyl (N-term) Carbamidomethyl (C) Phospho (STY) True False 1 2 Standard 7908 90647 0 115097 66197 0 48900 27259 26305 0 954 24 40 0 2 12072 740597 69088 9.3 10224 15 79707 44061 42998 55 61 13498 31 + 0.37097 0.56411 0.2955 0.49094 Total 258717 2892964 0 3534363 2251565 0 1282798 1049382 1016153 0 33229 30 45 0 2.6 53712 25602138 2987514 1542354 1505209 52 57 428996 28 0.3891 0.59444 0.28499 0.46817