0 NextStep F:\FPL\Zheng\20221227_Wisely_LH\20221205_ZS_SW_Wisely_CBS_7966_supt.pdResult 10755 Undefined 1dd56353-0bbc-4cc0-af9f-594ca4802de7 Consensus CWF_Basic_Annotation Result filtered for high confident peptides, with Annotations from Protein Annotation Server. http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys Identified or Quantified All Globally by Search Engine Type 0.05 0 0 ppm 0 0 0 0 0 0 0 0 0 0 Mascot: Ions Score; Sequest HT: XCorr; SEQUEST: XCorr; MSPepSearch: dot Score; MSPepSearch: rev-dot Score; MSPepSearch: MSPepSearch Score; PMI-Byonic: |Log Prob|; PMI-Byonic: Byonic Score; MS Amanda: Amanda Score Best match standard 75 http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys Automatic (Control peptide level error rate if possible) 0.01 0.05 0.01 0.05 q-Value Automatic False http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered Medium False 6 False 1 False False http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore True http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/Validated 0.01 0.05 http://thermo.magellan.com/owl/DataTypes/Filtered http://webservice.proteincenter.thermofisher.com/ProXweb/ 3 90 15 Biological Process Cellular Component Molecular Function None None None ### Master Proteins Default Filter:; ### Row Filter for TargetProtein:; ### Master is equal to Master; ###; ; 'magellan filter set' 1 'MasterFilter.filterset' Filter 'TargetProtein' FilterProperties 1 'FilterConditionProperties/FilterScope' 'FilterConditionProperties/FilterScopeValueMainGrid' 1 NARY_AND 1 = FilterConditionProperties 1 'NamedComparableFilterCondition/DisplayPropertyHint' 'Master' property 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' constant 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' ### Filter Set MasterProteinFilter.filterset contains the following filters:; ### Row Filter for TargetProtein:; ### Master is equal to Master; ###; ; 'magellan filter set' 1 'MasterProteinFilter.filterset' FiltersetProperties 1 'LastFileName' 'C:\Users\frank.berg\Desktop\MasterProteinFilter.filterset' Filter 'TargetProtein' 1 NARY_AND 1 = FilterConditionProperties 1 'NamedComparableFilterCondition/DisplayPropertyHint' 'Master' property 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' constant 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' Only unique peptides based on protein groups 1 InitialStep F:\FPL\Zheng\20221227_Wisely_LH\20221205_ZS_SW_Wisely_CBS_7966_supt.msf 10754 Running 2b71e1cf-e078-4f35-a139-08e37c3d0bf8 Processing PWF_Fusion_Basic_Sequest_2022Oct Basic processing workflow with score threshold validation to be used for searches of low complexity samples or employing a small FASTA database. Specify the FASTA database and any additional modifications. http://thermo.magellan.com/owl/DataTypes/CentroidData http://thermo.magellan.com/owl/DataTypes/ProfileData Use MS1 Precursor PD True True Automatic True Averagine True 0 0 0 0 0 0 350 Da 5000 Da 0 1 (Not specified) Is Not MS1 (Not specified) 0 1000 Is Full (Not specified) 1.5 Automatic ITMS MS2 CID + 60000 30000 2.5 Da 5.5 Da 12 100 Da http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys 20221205_Uniprot_Malasseziaglobosa_reference_withisoforms.fasta <Enzyme Version="1" Name="Trypsin" CleavageSites="KR" CleavageInhibitors="P" Offset="1" CleavageSpecificity="SpecificAtBothEnds" /> 3 6 150 10 10 ppm 0.6 Da False False True True True True 0 1 0 0 1 0 3 4 <Modification Version="2" AminoAcids="N, Q" Name="Deamidated" Abbreviation="Deamidated" ID="15" UnimodAccession="7" DeltaMass="0.98402" DeltaAverageMass="0.98480" IsSubstitution="False" LeavingGroup="" Substitution="H(-1) N(-1) O" PositionType="Any" /> <Modification Version="2" AminoAcids="M" Name="Oxidation" Abbreviation="Oxidation" ID="45" UnimodAccession="35" DeltaMass="15.99492" DeltaAverageMass="15.99940" IsSubstitution="False" LeavingGroup="" Substitution="O" PositionType="Any" /> <Modification Version="2" AminoAcids="N-Terminus" Name="Acetyl" Abbreviation="Acetyl" ID="2" UnimodAccession="1" DeltaMass="42.01057" DeltaAverageMass="42.03670" IsSubstitution="False" LeavingGroup="" Substitution="H(2) C(2) O" PositionType="Protein_N_Terminus" /> <Modification Version="2" AminoAcids="C" Name="Carbamidomethyl" Abbreviation="Carbamidomethyl" ID="8" UnimodAccession="4" DeltaMass="57.02146" DeltaAverageMass="57.05130" IsSubstitution="False" LeavingGroup="" Substitution="H(3) C(2) N O" PositionType="Any" /> True 3000 15 1.5 0.7 2 0.9 2.5 1.2 3 1.5 1.2 0.7 1.9 0.8 2.3 1 2.6 1.2 False 2.3 1.5 2EDB1DFD-FA0F-49B5-B8F4-EEF90C922903 39EFA907-9435-46E1-9AF8-1203D547387A True False http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys Concatenated q-Value D0E5CE92-ABF5-43F1-8D0F-C17FC9194D99 True 0.05 0 0.01 0.05 0 NextStep F:\FPL\Zheng\20221227_Wisely_LH\20221205_ZS_SW_Wisely_MGL_3331_supt.pdResult 10757 Waiting 527aa4d8-c4c1-498e-9cb9-b4a1a23a799b Consensus CWF_Basic_Annotation Result filtered for high confident peptides, with Annotations from Protein Annotation Server. http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys Identified or Quantified All Globally by Search Engine Type 0.05 0 0 ppm 0 0 0 0 0 0 0 0 0 0 Mascot: Ions Score; Sequest HT: XCorr; SEQUEST: XCorr; MSPepSearch: dot Score; MSPepSearch: rev-dot Score; MSPepSearch: MSPepSearch Score; PMI-Byonic: |Log Prob|; PMI-Byonic: Byonic Score; MS Amanda: Amanda Score Best match standard 75 http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys Automatic (Control peptide level error rate if possible) 0.01 0.05 0.01 0.05 q-Value Automatic False http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered Medium False 6 False 1 False False http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore True http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/Validated 0.01 0.05 http://thermo.magellan.com/owl/DataTypes/Filtered http://webservice.proteincenter.thermofisher.com/ProXweb/ 3 90 15 Biological Process Cellular Component Molecular Function None None None ### Master Proteins Default Filter:; ### Row Filter for TargetProtein:; ### Master is equal to Master; ###; ; 'magellan filter set' 1 'MasterFilter.filterset' Filter 'TargetProtein' FilterProperties 1 'FilterConditionProperties/FilterScope' 'FilterConditionProperties/FilterScopeValueMainGrid' 1 NARY_AND 1 = FilterConditionProperties 1 'NamedComparableFilterCondition/DisplayPropertyHint' 'Master' property 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' constant 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' ### Filter Set MasterProteinFilter.filterset contains the following filters:; ### Row Filter for TargetProtein:; ### Master is equal to Master; ###; ; 'magellan filter set' 1 'MasterProteinFilter.filterset' FiltersetProperties 1 'LastFileName' 'C:\Users\frank.berg\Desktop\MasterProteinFilter.filterset' Filter 'TargetProtein' 1 NARY_AND 1 = FilterConditionProperties 1 'NamedComparableFilterCondition/DisplayPropertyHint' 'Master' property 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' constant 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' Only unique peptides based on protein groups 1 InitialStep F:\FPL\Zheng\20221227_Wisely_LH\20221205_ZS_SW_Wisely_MGL_3331_supt.msf 10756 Running 90fa929a-4c10-47e3-bbc7-b2ddbcb70595 Processing PWF_Fusion_Basic_Sequest_2022Oct Basic processing workflow with score threshold validation to be used for searches of low complexity samples or employing a small FASTA database. Specify the FASTA database and any additional modifications. http://thermo.magellan.com/owl/DataTypes/CentroidData http://thermo.magellan.com/owl/DataTypes/ProfileData Use MS1 Precursor PD True True Automatic True Averagine True 0 0 0 0 0 0 350 Da 5000 Da 0 1 (Not specified) Is Not MS1 (Not specified) 0 1000 Is Full (Not specified) 1.5 Automatic ITMS MS2 CID + 60000 30000 2.5 Da 5.5 Da 12 100 Da http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys 20221205_Uniprot_Malasseziaglobosa_reference_withisoforms.fasta <Enzyme Version="1" Name="Trypsin" CleavageSites="KR" CleavageInhibitors="P" Offset="1" CleavageSpecificity="SpecificAtBothEnds" /> 3 6 150 10 10 ppm 0.6 Da False False True True True True 0 1 0 0 1 0 3 4 <Modification Version="2" AminoAcids="N, Q" Name="Deamidated" Abbreviation="Deamidated" ID="15" UnimodAccession="7" DeltaMass="0.98402" DeltaAverageMass="0.98480" IsSubstitution="False" LeavingGroup="" Substitution="H(-1) N(-1) O" PositionType="Any" /> <Modification Version="2" AminoAcids="M" Name="Oxidation" Abbreviation="Oxidation" ID="45" UnimodAccession="35" DeltaMass="15.99492" DeltaAverageMass="15.99940" IsSubstitution="False" LeavingGroup="" Substitution="O" PositionType="Any" /> <Modification Version="2" AminoAcids="N-Terminus" Name="Acetyl" Abbreviation="Acetyl" ID="2" UnimodAccession="1" DeltaMass="42.01057" DeltaAverageMass="42.03670" IsSubstitution="False" LeavingGroup="" Substitution="H(2) C(2) O" PositionType="Protein_N_Terminus" /> <Modification Version="2" AminoAcids="C" Name="Carbamidomethyl" Abbreviation="Carbamidomethyl" ID="8" UnimodAccession="4" DeltaMass="57.02146" DeltaAverageMass="57.05130" IsSubstitution="False" LeavingGroup="" Substitution="H(3) C(2) N O" PositionType="Any" /> True 3000 15 1.5 0.7 2 0.9 2.5 1.2 3 1.5 1.2 0.7 1.9 0.8 2.3 1 2.6 1.2 False 2.3 1.5 2EDB1DFD-FA0F-49B5-B8F4-EEF90C922903 39EFA907-9435-46E1-9AF8-1203D547387A True False http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys Concatenated q-Value D0E5CE92-ABF5-43F1-8D0F-C17FC9194D99 True 0.05 0 0.01 0.05 0 NextStep F:\FPL\Zheng\20221227_Wisely_LH\20221205_ZS_SW_Wisely_MGL_3331_ppt.pdResult 10759 Waiting 27a8ced3-b620-4d43-a7c5-d8fea294f245 Consensus CWF_Basic_Annotation Result filtered for high confident peptides, with Annotations from Protein Annotation Server. http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys Identified or Quantified All Globally by Search Engine Type 0.05 0 0 ppm 0 0 0 0 0 0 0 0 0 0 Mascot: Ions Score; Sequest HT: XCorr; SEQUEST: XCorr; MSPepSearch: dot Score; MSPepSearch: rev-dot Score; MSPepSearch: MSPepSearch Score; PMI-Byonic: |Log Prob|; PMI-Byonic: Byonic Score; MS Amanda: Amanda Score Best match standard 75 http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys Automatic (Control peptide level error rate if possible) 0.01 0.05 0.01 0.05 q-Value Automatic False http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered Medium False 6 False 1 False False http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/Filtered http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore True http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/Validated 0.01 0.05 http://thermo.magellan.com/owl/DataTypes/Filtered http://webservice.proteincenter.thermofisher.com/ProXweb/ 3 90 15 Biological Process Cellular Component Molecular Function None None None ### Master Proteins Default Filter:; ### Row Filter for TargetProtein:; ### Master is equal to Master; ###; ; 'magellan filter set' 1 'MasterFilter.filterset' Filter 'TargetProtein' FilterProperties 1 'FilterConditionProperties/FilterScope' 'FilterConditionProperties/FilterScopeValueMainGrid' 1 NARY_AND 1 = FilterConditionProperties 1 'NamedComparableFilterCondition/DisplayPropertyHint' 'Master' property 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' constant 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' ### Filter Set MasterProteinFilter.filterset contains the following filters:; ### Row Filter for TargetProtein:; ### Master is equal to Master; ###; ; 'magellan filter set' 1 'MasterProteinFilter.filterset' FiltersetProperties 1 'LastFileName' 'C:\Users\frank.berg\Desktop\MasterProteinFilter.filterset' Filter 'TargetProtein' 1 NARY_AND 1 = FilterConditionProperties 1 'NamedComparableFilterCondition/DisplayPropertyHint' 'Master' property 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' constant 'Thermo.PD.EntityDataFramework.MasterProteinAssessment, Thermo.Magellan.EntityDataFramework' 'IsMasterProtein' Only unique peptides based on protein groups 1 InitialStep F:\FPL\Zheng\20221227_Wisely_LH\20221205_ZS_SW_Wisely_MGL_3331_ppt.msf 10758 Running f0db2887-7ebf-4bd7-a02a-7e3b5108d800 Processing PWF_Fusion_Basic_Sequest_2022Oct Basic processing workflow with score threshold validation to be used for searches of low complexity samples or employing a small FASTA database. Specify the FASTA database and any additional modifications. http://thermo.magellan.com/owl/DataTypes/CentroidData http://thermo.magellan.com/owl/DataTypes/ProfileData Use MS1 Precursor PD True True Automatic True Averagine True 0 0 0 0 0 0 350 Da 5000 Da 0 1 (Not specified) Is Not MS1 (Not specified) 0 1000 Is Full (Not specified) 1.5 Automatic ITMS MS2 CID + 60000 30000 2.5 Da 5.5 Da 12 100 Da http://thermo.magellan.com/owl/DataTypes/ScoredWithNativeScore http://thermo.magellan.com/owl/DataTypes/WithDecoys http://thermo.magellan.com/owl/DataTypes/WithDecoys 20221205_Uniprot_Malasseziaglobosa_reference_withisoforms.fasta <Enzyme Version="1" Name="Trypsin" CleavageSites="KR" CleavageInhibitors="P" Offset="1" CleavageSpecificity="SpecificAtBothEnds" /> 3 6 150 10 10 ppm 0.6 Da False False True True True True 0 1 0 0 1 0 3 4 <Modification Version="2" AminoAcids="N, Q" Name="Deamidated" Abbreviation="Deamidated" ID="15" UnimodAccession="7" DeltaMass="0.98402" DeltaAverageMass="0.98480" IsSubstitution="False" LeavingGroup="" Substitution="H(-1) N(-1) O" PositionType="Any" /> <Modification Version="2" AminoAcids="M" Name="Oxidation" Abbreviation="Oxidation" ID="45" UnimodAccession="35" DeltaMass="15.99492" DeltaAverageMass="15.99940" IsSubstitution="False" LeavingGroup="" Substitution="O" PositionType="Any" /> <Modification Version="2" AminoAcids="N-Terminus" Name="Acetyl" Abbreviation="Acetyl" ID="2" UnimodAccession="1" DeltaMass="42.01057" DeltaAverageMass="42.03670" IsSubstitution="False" LeavingGroup="" Substitution="H(2) C(2) O" PositionType="Protein_N_Terminus" /> <Modification Version="2" AminoAcids="C" Name="Carbamidomethyl" Abbreviation="Carbamidomethyl" ID="8" UnimodAccession="4" DeltaMass="57.02146" DeltaAverageMass="57.05130" IsSubstitution="False" LeavingGroup="" Substitution="H(3) C(2) N O" PositionType="Any" /> True 3000 15 1.5 0.7 2 0.9 2.5 1.2 3 1.5 1.2 0.7 1.9 0.8 2.3 1 2.6 1.2 False 2.3 1.5 2EDB1DFD-FA0F-49B5-B8F4-EEF90C922903 39EFA907-9435-46E1-9AF8-1203D547387A True False http://thermo.magellan.com/owl/DataTypes/ScoredWithPEP http://thermo.magellan.com/owl/DataTypes/ScoredWithQValue http://thermo.magellan.com/owl/DataTypes/Validated http://thermo.magellan.com/owl/DataTypes/WithDecoys Concatenated q-Value D0E5CE92-ABF5-43F1-8D0F-C17FC9194D99 True 0.05 0 0.01 0.05