Raw file	Experiment	Enzyme	Enzyme mode	Enzyme first search	Enzyme mode first search	Use enzyme first search	Variable modifications	Fixed modifications	Multi modifications	Variable modifications first search	Use variable modifications first search	Requantify	Multiplicity	Max. missed cleavages	Labels0	LC-MS run type	Time-dependent recalibration	MS	MS/MS	MS3	MS/MS submitted	MS/MS submitted (SIL)	MS/MS submitted (ISO)	MS/MS submitted (PEAK)	MS/MS identified	MS/MS identified (SIL)	MS/MS identified (ISO)	MS/MS identified (PEAK)	MS/MS identified [%]	MS/MS identified (SIL) [%]	MS/MS identified (ISO) [%]	MS/MS identified (PEAK) [%]	Peptide sequences identified	Peaks	Peaks sequenced	Peaks sequenced [%]	Peaks repeatedly sequenced	Peaks repeatedly sequenced [%]	Isotope patterns	Isotope patterns sequenced	Isotope patterns sequenced (z>1)	Isotope patterns sequenced [%]	Isotope patterns sequenced (z>1) [%]	Isotope patterns repeatedly sequenced	Isotope patterns repeatedly sequenced [%]	Recalibrated	Av. absolute mass deviation [ppm]	Mass standard deviation [ppm]	Av. absolute mass deviation [mDa]	Mass standard deviation [mDa]	Label free norm param
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_227_1	LDS1_1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2596	19060	0	25389	12731	0	12658	3728	3487	0	241	15	27	0	1.9	2727	179503	16632	9.3	812	4.9	26229	11134	10530	42	51	1155	10	+	0.60043	0.87236	0.36552	0.56158	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_227_2	LDS1_2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2605	18916	0	25230	12602	0	12628	3770	3535	0	235	15	28	0	1.9	2742	183132	16642	9.1	756	4.5	26578	11012	10392	41	50	1185	11	+	0.57784	0.83335	0.34991	0.53029	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_228_1	LDS1_1	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		3014	17034	0	24346	9722	0	14624	903	865	0	38	3.7	8.9	0	0.26	573	168801	14358	8.5	844	5.9	18957	8152	7208	43	65	1234	15	+	0.45961	0.68716	0.28483	0.4491	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_228_2	LDS1_2	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		3025	16921	0	24011	9831	0	14180	910	889	0	21	3.8	9	0	0.15	571	169526	14418	8.5	801	5.6	19172	8277	7320	43	65	1223	15	+	0.48199	0.6966	0.292	0.43972	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_229_1	LDS1_1	ProAlanase	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		3392	15044	0	22290	7794	0	14496	170	167	0	3	0.76	2.1	0	0.021	143	189420	12771	6.7	808	6.3	14767	6431	5337	44	71	1033	16	+	0.34604	0.51773	0.20046	0.31995	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_229_2	LDS1_2	ProAlanase	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		3240	15622	0	23020	8222	0	14798	158	156	0	2	0.69	1.9	0	0.014	141	188183	13437	7.1	820	6.1	16329	6866	5762	42	70	1032	15	+	0.32535	0.48294	0.19537	0.3085	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_230_1	LSD1_Nek6_1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2645	18634	0	24541	12725	0	11816	3742	3540	0	202	15	28	0	1.7	2696	177485	16337	9.2	746	4.6	25663	11077	10533	43	52	1241	11	+	0.59753	0.86006	0.37329	0.56938	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_230_2	LSD1_Nek6_2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2715	18251	0	24009	12489	0	11520	3764	3554	0	210	16	28	0	1.8	2737	176331	16053	9.1	767	4.8	25388	10858	10361	43	51	1280	12	+	0.59571	0.85036	0.37182	0.5658	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_231_1	LSD1_Nek6_1	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2968	17167	0	24689	9645	0	15044	1046	1013	0	33	4.2	11	0	0.22	614	172941	14116	8.2	822	5.8	17876	8024	7257	45	63	1274	16	+	0.47654	0.68744	0.32815	0.51544	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_231_2	LSD1_Nek6_2	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2899	17505	0	25308	9702	0	15606	1035	999	0	36	4.1	10	0	0.23	612	163159	14231	8.7	769	5.4	18079	8086	7318	45	62	1264	16	+	0.45818	0.67901	0.30681	0.4826	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_232_1	LSD1_Nek6_1	ProAlanase	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		3898	11846	0	17279	6411	0	10868	298	265	0	33	1.7	4.1	0	0.3	236	158344	9821	6.2	418	4.3	17301	5634	4782	33	50	650	12	+	0.55809	0.77502	0.35704	0.50898	
210607_C21_022_OE480_BSA_Rev00_#21_031_PAP21_232_2	LSD1_Nek6_2	ProAlanase	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		2970	16941	0	23545	10335	0	13210	583	565	0	18	2.5	5.5	0	0.14	400	173702	14793	8.5	790	5.3	19314	8805	7817	46	65	1184	13	+	0.46983	0.66801	0.28217	0.4174	
210607_C21_007_OE480_BSA_Rev03_#21_031_PAP21_269	LDS1_3	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		1371	13274	0	17717	8831	0	8886	587	559	0	28	3.3	6.3	0	0.32	414	160853	11170	6.9	763	6.8	20655	7559	7104	37	51	767	10	+	0.27855	0.40145	0.18287	0.28323	
210607_C21_007_OE480_BSA_Rev03_#21_031_PAP21_269_2	LDS1_4	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		1378	13269	0	17533	9005	0	8528	587	569	0	18	3.3	6.3	0	0.21	415	155580	11100	7.1	726	6.5	21031	7712	7270	37	51	771	10	+	0.27343	0.38554	0.1811	0.26643	
210607_C21_007_OE480_BSA_Rev03_#21_031_PAP21_270	LSD1_Nek6_3	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		1479	13279	0	17467	9091	0	8376	635	605	0	30	3.6	6.7	0	0.36	457	177107	11301	6.4	692	6.1	22377	7720	7219	34	51	793	10	+	0.30588	0.41913	0.19429	0.28186	
210607_C21_007_OE480_BSA_Rev03_#21_031_PAP21_270_2	LSD1_Nek6_4	GluC	Specific			False	Oxidation (M);Acetyl (Protein N-term);Phospho (STY)	Carbamidomethyl (C)			True	False	1	2		Standard		1413	13712	0	18026	9398	0	8628	677	637	0	40	3.8	6.8	0	0.46	466	174254	11596	6.7	800	6.9	22694	8001	7570	35	49	832	10	+	0.33544	0.46803	0.22169	0.33498	
Total																		41608	256475	0	354400	158534	0	195866	22593	21405	0	1188	6.4	14	0	0.61	5115	2768321					332410	135348	123780	41	55	16918	12		0.53439	0.78317	0.33272	0.5154