MTD mzTab-version 1.0 MTD mzTab-mode Complete MTD mzTab-type Identification MTD description JPST000508 phospho -- main MTD ms_run[1]-location D:\JobRequest\ResultFiles\20220104\20220104170124317195^fe80..dc7a.184c.1632.7be4^jpost@jpost.jpost\PeakList.MaxQuantPlist1\mai100630_011STLK3_STK39.maxq.txt MTD ms_run[2]-location D:\JobRequest\ResultFiles\20220104\20220104170124317195^fe80..dc7a.184c.1632.7be4^jpost@jpost.jpost\Psearch.MaxQuantExec1\mai100630_011STLK3_STK39.maxqFin.txt MTD software[1] [MS, MS:1001583, MaxQuant, 1.6.17.0] MTD software[1]-setting Taxon=userFasta.sprot_human_20211018 MTD software[1]-setting enzymes=Trypsin/P MTD software[1]-setting enzymeMode=0 MTD software[1]-setting fixedModifications=Carbamidomethyl (C) MTD software[1]-setting variableModifications=Dimethyl:2H(4)13C(2) (K),Dimethyl:2H(4)13C(2) (N-term),Oxidation (M),Phospho (STY) MTD software[1]-setting maxMissedCleavages=2 MTD software[1]-setting lcmsRunType=Standard MTD software[1]-setting firstSearchTol=20 MTD software[1]-setting mainSearchTol=4.5 MTD software[2] [MS, MS:1001476, X!Tandem, 2015.04.01.1] MTD software[2]-setting DB=userFasta.sprot_human_20211018 MTD software[2]-setting CLE=[RK]|{} MTD software[2]-setting MODS=Carbamidomethyl (C) MTD software[2]-setting IT_MODS=Oxidation (M),Phospho (S),Phospho (T),Phospho (Y),Dimethyl:2H(4)13C(2) (K),Dimethyl:2H(4)13C(2) (N-term) MTD software[2]-setting TOL(-)=10 MTD software[2]-setting TOL(+)=10 MTD software[2]-setting TOLU=ppm MTD software[2]-setting ITOL=300 MTD software[2]-setting ITOLU=ppm MTD software[2]-setting PEP_ISOTOPE_ERROR=yes MTD software[2]-setting PFA=2 MTD software[3] [MS, MS:1002251, Comet, 2019.01 rev. 5] MTD software[3]-setting Taxon=userFasta.sprot_human_20211018 MTD software[3]-setting search_enzyme_number=2 MTD software[3]-setting FixMod=Carbamidomethyl (C) MTD software[3]-setting VarMod=Dimethyl:2H(4)13C(2) (K),Dimethyl:2H(4)13C(2) (N-term),Oxidation (M),Phospho (S),Phospho (T),Phospho (Y) MTD software[3]-setting max_variable_mods_in_peptide=5 MTD software[3]-setting allowed_missed_cleavage=2 MTD software[3]-setting peptide_mass_tolerance=10 MTD software[3]-setting peptide_mass_units=2 MTD software[3]-setting fragment_bin_tol=0.02 MTD software[3]-setting fragment_bin_offset=0.0 MTD fixed_mod[1] [UNIMOD, UNIMOD:4, Carbamidomethyl,] MTD fixed_mod[1]-site C MTD fixed_mod[1]-position Anywhere MTD variable_mod[1] [UNIMOD, UNIMOD:510, Dimethyl:2H(4)13C(2),] MTD variable_mod[1]-site K MTD variable_mod[1]-position Anywhere MTD variable_mod[2] [UNIMOD, UNIMOD:510, Dimethyl:2H(4)13C(2),] MTD variable_mod[2]-site N-term MTD variable_mod[2]-position Any N-term MTD variable_mod[3] [UNIMOD, UNIMOD:35, Oxidation,] MTD variable_mod[3]-site M MTD variable_mod[3]-position Anywhere MTD variable_mod[4] [UNIMOD, UNIMOD:21, Phospho,] MTD variable_mod[4]-site S MTD variable_mod[4]-position Anywhere MTD variable_mod[5] [UNIMOD, UNIMOD:21, Phospho,] MTD variable_mod[5]-site T MTD variable_mod[5]-position Anywhere MTD variable_mod[6] [UNIMOD, UNIMOD:21, Phospho,] MTD variable_mod[6]-site Y MTD variable_mod[6]-position Anywhere MTD protein_search_engine_score[1] [http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore] MTD psm_search_engine_score[1] [http://rdf.jpostdb.org/ontology/jpost.owl#JpostScore] PRH accession description taxid species database database_version search_engine best_search_engine_score[1] ambiguity_members modifications protein_coverage search_engine_score[1]_ms_run[1] num_psms_ms_run[1] num_peptides_distinct_ms_run[1] num_peptides_unique_ms_run[1] PRT sp|P61981|1433G_HUMAN 14-3-3 protein gamma OS=Homo sapiens OX=9606 GN=YWHAG PE=1 SV=2 null null userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001476, X!Tandem, ] 35.0 null 228-UNIMOD:510 0.09 35.0 1 1 1 PRT sp|O95747|OXSR1_HUMAN Serine/threonine-protein kinase OSR1 OS=Homo sapiens OX=9606 GN=OXSR1 PE=1 SV=1 null null userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001583, MaxQuant, ] 20.0 null 331-UNIMOD:510,339-UNIMOD:21,351-UNIMOD:510 0.04 20.0 1 1 1 PRT sp|P62258-2|1433E_HUMAN Isoform SV of 14-3-3 protein epsilon OS=Homo sapiens OX=9606 GN=YWHAE null null userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001583, MaxQuant, ] 15.0 null 223-UNIMOD:510 0.05 15.0 1 1 1 PRT sp|P11021|BIP_HUMAN Endoplasmic reticulum chaperone BiP OS=Homo sapiens OX=9606 GN=HSPA5 PE=1 SV=2 null null userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1002251, Comet, ] 15.0 null 50-UNIMOD:510 0.02 15.0 1 1 1 PRT sp|Q8WXX0|DYH7_HUMAN Dynein axonemal heavy chain 7 OS=Homo sapiens OX=9606 GN=DNAH7 PE=1 SV=2 null null userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001476, X!Tandem, ] 14.0 null 1575-UNIMOD:510,1577-UNIMOD:4,1579-UNIMOD:21,1589-UNIMOD:21,1591-UNIMOD:510 0.00 14.0 1 1 1 PRT sp|Q5VX52|SPAT1_HUMAN Spermatogenesis-associated protein 1 OS=Homo sapiens OX=9606 GN=SPATA1 PE=2 SV=4 null null userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1002251, Comet, ] 13.0 null 346-UNIMOD:21,349-UNIMOD:21,355-UNIMOD:21,356-UNIMOD:35 0.04 13.0 1 1 1 PSH sequence PSM_ID accession unique database database_version search_engine search_engine_score[1] modifications spectra_ref retention_time charge exp_mass_to_charge calc_mass_to_charge pre post start end PSM DNLTLWTSDQQDDDGGEGNN 1 sp|P61981|1433G_HUMAN 1 userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001476, X!Tandem, ] 35.0 1-UNIMOD:510 ms_run[1]:scan=2355 48.339645000000004 2 2226.9288 2226.9356 R - 228 248 PSM TEDGGWEWSDDEFDEESEEGK 2 sp|O95747|OXSR1_HUMAN 1 userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001583, MaxQuant, ] 20.0 1-UNIMOD:510,9-UNIMOD:21,21-UNIMOD:510 ms_run[2]:scan=2529 51.773 3 2623.0072 2623.0072 K A 331 352 PSM EALQDVEDENQ 3 sp|P62258-2|1433E_HUMAN 1 userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001583, MaxQuant, ] 15.0 1-UNIMOD:510 ms_run[2]:scan=1360 27.86 2 1322.605 1322.6050 K - 223 234 PSM VEIIANDQGNR 4 sp|P11021|BIP_HUMAN 1 userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1002251, Comet, ] 15.0 1-UNIMOD:510 ms_run[1]:scan=1081 22.153401666666667 2 1261.682439 1261.683881 R I 50 61 PSM DNCASMNLQMTAFFSEK 5 sp|Q8WXX0|DYH7_HUMAN 1 userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1001476, X!Tandem, ] 14.0 1-UNIMOD:510,3-UNIMOD:4,5-UNIMOD:21,15-UNIMOD:21,17-UNIMOD:510 ms_run[1]:scan=2356 48.34748666666666 2 2220.8972 2220.8963 K I 1575 1592 PSM YLETKKVTASMEEVLTK 6 sp|Q5VX52|SPAT1_HUMAN 1 userFasta.sprot_human_20211018 userFasta.sprot_human_20211018 [MS, MS:1002251, Comet, ] 13.0 1-UNIMOD:21,4-UNIMOD:21,10-UNIMOD:21,11-UNIMOD:35 ms_run[1]:scan=2355 48.339645000000004 2 2226.929321 2224.938088 K L 346 363