Raw file	Experiment	Fraction	Enzyme	Enzyme mode	Enzyme first search	Enzyme mode first search	Use enzyme first search	Variable modifications	Fixed modifications	Multi modifications	Variable modifications first search	Use variable modifications first search	Requantify	Multiplicity	Max. missed cleavages	Labels0	LC-MS run type	Time-dependent recalibration	MS	MS/MS	MS3	MS/MS Submitted	MS/MS Submitted (SIL)	MS/MS Submitted (ISO)	MS/MS Submitted (PEAK)	MS/MS Identified	MS/MS Identified (SIL)	MS/MS Identified (ISO)	MS/MS Identified (PEAK)	MS/MS Identified [%]	MS/MS Identified (SIL) [%]	MS/MS Identified (ISO) [%]	MS/MS Identified (PEAK) [%]	Peptide Sequences Identified	Peaks	Peaks Sequenced	Peaks Sequenced [%]	Peaks Repeatedly Sequenced	Peaks Repeatedly Sequenced [%]	Isotope Patterns	Isotope Patterns Sequenced	Isotope Patterns Sequenced (z>1)	Isotope Patterns Sequenced [%]	Isotope Patterns Sequenced (z>1) [%]	Isotope Patterns Repeatedly Sequenced	Isotope Patterns Repeatedly Sequenced [%]	Recalibrated	Av. Absolute Mass Deviation [ppm]	Mass Standard Deviation [ppm]	Av. Absolute Mass Deviation [mDa]	Mass Standard Deviation [mDa]
20211116_3428_TMUAki_TMT10x_P_F1	P	1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		6966	28884	0	41404	16364	0	25040	8022	7069	0	953	19	43	0	3.8	4239	408337	21605	5.3	2392	11	30712	11722	11120	38	49	3503	30	+	0.3566	0.54502	128150	138830
20211116_3429_TMUAki_TMT10x_P_F2	P	2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		5923	31672	0	44653	18691	0	25962	10277	8959	0	1318	23	48	0	5.1	4921	414441	24259	5.9	2371	9.8	39979	14106	13625	35	41	3544	25	+	0.42621	0.66643	128070	137800
20211116_3430_TMUAki_TMT10x_P_F3	P	3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		5360	33774	0	50538	17010	0	33528	10451	8832	0	1619	21	52	0	4.8	4340	360015	24978	6.9	3279	13	30260	11657	10968	39	49	3797	33	+	0.42562	0.66976	131030	140060
20211116_3431_TMUAki_TMT10x_P_F4	P	4	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		6796	29616	0	41999	17233	0	24766	10293	8877	0	1416	25	52	0	5.7	5418	363352	22484	6.2	2434	11	30424	12629	12094	42	51	3602	29	+	0.35868	0.54864	133190	142740
20211116_3433_TMUAki_TMT10x_P_F5	P	5	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7311	28353	0	41209	15497	0	25712	9478	7978	0	1500	23	51	0	5.8	4682	425735	20688	4.9	2390	12	30412	10898	10467	36	49	3487	32	+	0.34695	0.52886	138710	148230
20211116_3434_TMUAki_TMT10x_P_F6	P	6	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		8054	25815	0	36909	14721	0	22188	8816	7638	0	1178	24	52	0	5.3	4187	488057	19182	3.9	2417	13	34897	9949	9433	29	46	3493	35	+	0.36665	0.56662	142310	151320
20211116_3435_TMUAki_TMT10x_P_F7	P	7	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		6819	29290	0	41757	16823	0	24934	9572	8358	0	1214	23	50	0	4.9	4042	507190	21963	4.3	2643	12	44925	11800	11263	26	39	3745	32	+	0.38613	0.59632	145220	154600
20211116_3436_TMUAki_TMT10x_P_F8	P	8	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7470	27347	0	38839	15855	0	22984	8280	7131	0	1149	21	45	0	5	3782	498050	20660	4.1	2539	12	41105	11142	10684	27	42	3520	32	+	0.39734	0.61152	143410	152630
20211116_3439_TMUAki_TMT10x_S_F1	S	1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		6606	30143	0	43611	16675	0	26936	8225	7217	0	1008	19	43	0	3.7	4125	431970	22480	5.2	2759	12	34682	11729	11350	34	47	3494	30	+	0.37604	0.58972	126620	137090
20211116_3440_TMUAki_TMT10x_S_F2	S	2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7606	27340	0	39161	15519	0	23642	8269	7153	0	1116	21	46	0	4.7	4099	481091	20562	4.3	2315	11	39852	11139	10621	28	42	3324	30	+	0.41333	0.6476	130180	139680
20211116_3441_TMUAki_TMT10x_S_F3	S	3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		6002	31646	0	44027	19265	0	24762	10440	9035	0	1405	24	47	0	5.7	5514	406843	24656	6.1	2473	10	44868	14435	13955	32	45	3674	25	+	0.40707	0.63746	125620	134900
20211116_3442_TMUAki_TMT10x_S_F4	S	4	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7391	28015	0	40854	15176	0	25678	9355	8011	0	1344	23	53	0	5.2	4604	462354	20800	4.5	2435	12	36282	10633	10126	29	47	3375	32	+	0.41198	0.62833	132920	142220
20211116_3444_TMUAki_TMT10x_S_F5	S	5	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7865	26532	0	38275	14789	0	23486	9310	7905	0	1405	24	53	0	6	4705	461725	19559	4.2	2360	12	33505	10310	9938	31	47	3366	33	+	0.37335	0.57368	137020	146300
20211116_3445_TMUAki_TMT10x_S_F6	S	6	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		7686	26859	0	38237	15481	0	22756	8821	7684	0	1137	23	50	0	5	4273	468617	20400	4.4	2205	11	35758	11143	10615	31	46	3317	30	+	0.40435	0.60782	139970	148880
20211116_3446_TMUAki_TMT10x_S_F7	S	7	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		6703	29750	0	43063	16437	0	26626	9091	7885	0	1206	21	48	0	4.5	3884	570701	22443	3.9	2506	11	53573	11792	11233	22	30	3407	29	+	0.44823	0.68404	143890	153230
20211116_3447_TMUAki_TMT10x_S_F8	S	8	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term);Carbamidomethyl (C)	Carbamidomethyl (C)			False	False	1	2		Reporter ion MS2		8058	25821	0	37293	14349	0	22944	7442	6387	0	1055	20	45	0	4.6	3594	476768	19016	4	2418	13	33232	9949	9556	30	47	3204	32	+	0.37323	0.56412	142320	151620
Total																			112616	460857	0	661829	259885	0	401944	146142	126119	0	20023	22	49	0	5	20623	7225246					594466	185033	177048	31	44	55852	30		0.393	0.60783	135260	144860