Raw file	Fraction	Enzyme	Enzyme mode	Enzyme first search	Enzyme mode first search	Use enzyme first search	Variable modifications	Fixed modifications	Multi modifications	Variable modifications first search	Use variable modifications first search	Requantify	Multiplicity	Max. missed cleavages	Labels0	LC-MS run type	Time-dependent recalibration	MS	MS/MS	MS3	MS/MS submitted	MS/MS submitted (SIL)	MS/MS submitted (ISO)	MS/MS submitted (PEAK)	MS/MS identified	MS/MS identified (SIL)	MS/MS identified (ISO)	MS/MS identified (PEAK)	MS/MS identified [%]	MS/MS identified (SIL) [%]	MS/MS identified (ISO) [%]	MS/MS identified (PEAK) [%]	Peptide sequences identified	Peaks	Peaks sequenced	Peaks sequenced [%]	Peaks repeatedly sequenced	Peaks repeatedly sequenced [%]	Isotope patterns	Isotope patterns sequenced	Isotope patterns sequenced (z>1)	Isotope patterns sequenced [%]	Isotope patterns sequenced (z>1) [%]	Isotope patterns repeatedly sequenced	Isotope patterns repeatedly sequenced [%]	Recalibrated	Av. absolute mass deviation [ppm]	Mass standard deviation [ppm]	Av. absolute mass deviation [mDa]	Mass standard deviation [mDa]
20230619_1880_Aki_TMTPro18x_K_F1	1	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8160	18678	0	24396	12960	0	11436	5897	5299	0	598	24	41	0	5.2	5254	534091	14883	2.8	564	3.8	56875	11932	11641	21	28	927	7.8	+	0.53236	0.77003	163840	176030
20230619_1881_Aki_TMTPro18x_K_F2	2	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8450	18796	0	25158	12434	0	12724	6318	5569	0	749	25	45	0	5.9	5596	517352	14712	2.8	600	4.1	53219	11407	11165	21	28	926	8.1	+	0.58746	0.8702	170850	182670
20230619_1882_Aki_TMTPro18x_K_F3	3	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8453	18539	0	24782	12296	0	12486	6377	5643	0	734	26	46	0	5.9	5673	535857	14592	2.7	574	3.9	55507	11259	10988	20	26	933	8.3	+	0.56685	0.84939	176420	188390
20230619_1883_Aki_TMTPro18x_K_F4	4	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8555	18289	0	24398	12180	0	12218	6453	5731	0	722	26	47	0	5.9	5767	527938	14357	2.7	575	4	54935	11120	10855	20	26	942	8.5	+	0.56525	0.83737	177270	189030
20230619_1885_Aki_TMTPro18x_K_F5	5	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8566	18228	0	24423	12033	0	12390	6039	5333	0	706	25	44	0	5.7	5422	525969	14338	2.7	597	4.2	53956	10973	10731	20	26	941	8.6	+	0.59719	0.87214	173390	185140
20230619_1886_Aki_TMTPro18x_K_F6	6	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8571	18460	0	24913	12007	0	12906	6033	5389	0	644	24	45	0	5	5280	516967	14336	2.8	621	4.3	52251	10972	10715	21	26	929	8.5	+	0.63383	0.93678	179040	190870
20230619_1887_Aki_TMTPro18x_K_F7	7	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8482	18376	0	24674	12078	0	12596	5918	5249	0	669	24	43	0	5.3	5270	527222	14378	2.7	602	4.2	52428	11066	10779	21	27	906	8.2	+	0.55493	0.81263	180900	192700
20230619_1888_Aki_TMTPro18x_K_F8	8	Trypsin/P	Specific			False	Oxidation (M);Acetyl (Protein N-term)	Carbamidomethyl (C)			False	False	1	2		Reporter MS2		8613	18025	0	24398	11652	0	12746	5546	4916	0	630	23	42	0	4.9	4920	535850	13900	2.6	613	4.4	52080	10630	10369	20	26	928	8.7	+	0.57834	0.8621	181690	193530
Total																		67850	147391	0	197142	97640	0	99502	48581	43129	0	5452	25	44	0	5.5	29825	4221246					431251	89359	87243	21	26	7432	8.3		0.57691	0.85242	175350	187300