Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param 240321_C24_007_OE480_PRM_GNE_2_#24_009_GNE410 NIL rep1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 14378 3539 0 5333 1745 0 3588 740 611 0 129 14 35 0 3.6 40 1384479 844 0.061 576 68 101338 283 234 0.28 0.32 218 77 + 0.2005 0.28066 0.12744 0.18128 240321_C24_007_OE480_PRM_GNE_2_#24_009_GNE411 NIL rep2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 14595 2083 0 2942 1224 0 1718 347 334 0 13 12 27 0 0.76 22 1525318 519 0.034 353 68 119454 232 214 0.19 0.24 177 76 + 0.34316 0.4528 0.25586 0.36646 240321_C24_007_OE480_PRM_GNE_2_#24_009_GNE412 NIL rep3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 14548 2447 0 3540 1354 0 2186 393 356 0 37 11 26 0 1.7 32 1551322 575 0.037 398 69 130221 246 230 0.19 0.22 185 75 + 0.22988 0.33471 0.15135 0.26633 240321_C24_007_OE480_PRM_GNE_2_#24_009_GNE413 dog1-1 rep1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 14457 3049 0 4475 1623 0 2852 581 516 0 65 13 32 0 2.3 26 1448647 687 0.047 481 70 107436 234 207 0.22 0.27 179 76 + 0.18685 0.27624 0.11647 0.17229 240321_C24_007_OE480_PRM_GNE_2_#24_009_GNE414 dog1-1 rep2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 14644 1762 0 2479 1045 0 1434 451 390 0 61 18 37 0 4.3 20 1517140 423 0.028 268 63 117460 175 163 0.15 0.18 129 74 + 0.34742 0.57025 0.21495 0.33246 240321_C24_007_OE480_PRM_GNE_2_#24_009_GNE416 dog1-1 rep3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term);Phospho (STY) Carbamidomethyl (C) False False 1 2 Standard 14459 3042 0 4514 1570 0 2944 926 674 0 252 21 43 0 8.6 35 1449516 714 0.049 451 63 115065 270 257 0.23 0.29 195 72 + 0.30982 0.40191 0.2113 0.2818 240321_C24_007_OE480_Thermo_Rev06_#24_009_GNE410 NIL rep1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5903 69921 0 96518 43322 0 53196 2939 2898 0 41 3 6.7 0 0.077 2388 524511 61256 12 3137 5.1 70192 35239 32443 50 59 6236 18 + 0.34735 0.49121 0.23911 0.34736 240321_C24_007_OE480_Thermo_Rev06_#24_009_GNE411 NIL rep2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5754 71290 0 97598 44980 0 52618 2926 2869 0 57 3 6.4 0 0.11 2338 543199 61959 11 3072 5 74257 36198 33647 49 56 6694 18 + 0.38517 0.54538 0.26926 0.39932 240321_C24_007_OE480_Thermo_Rev06_#24_009_GNE412 NIL rep3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5611 72379 0 96652 48106 0 48546 4861 4748 0 113 5 9.9 0 0.23 3837 565850 63944 11 2647 4.1 82853 39704 37450 48 54 6630 17 + 0.38773 0.54733 0.27129 0.40158 240321_C24_007_OE480_Thermo_Rev06_#24_009_GNE413 dog1-1 rep1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5793 70930 0 97725 44131 0 53594 3224 3170 0 54 3.3 7.2 0 0.1 2613 530361 62251 12 2978 4.8 72211 35751 32875 50 58 6458 18 + 0.38839 0.56299 0.27092 0.41776 240321_C24_007_OE480_Thermo_Rev06_#24_009_GNE414 dog1-1 rep2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5838 70631 0 96702 44554 0 52148 3367 3297 0 70 3.5 7.4 0 0.13 2645 543209 61394 11 3050 5 73608 35862 33567 49 56 6615 18 + 0.39697 0.56584 0.28066 0.4188 240321_C24_007_OE480_Thermo_Rev06_#24_009_GNE416 dog1-1 rep3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5886 70153 0 93356 46950 0 46406 3874 3815 0 59 4.1 8.1 0 0.13 3194 541060 62594 12 2677 4.3 76975 38729 36451 50 58 6452 17 + 0.39398 0.56825 0.27437 0.41855 Total 121866 441226 0 601834 280604 0 321230 24629 23678 0 951 4.1 8.4 0 0.3 5326 12124612 1141070 222923 207738 20 23 40168 18 0.38348 0.54786 0.26777 0.40184