Path: 2023-01-23_21-12-28_One-column-separation
Procedure: One column separation
Argument list:
uses_trap = False
uses_separator = True
is_isocratic = False
analysis_time = 1800
oven_temperature = 40
sample_volume = 1.25
sample_position = Slot2:37
presample_solvent_volume = 2
presample_air_volume = 0.5
postsample_air_volume = 1
postsample_solvent_volume = 2
sample_aspirate_speed = 5
sample_postaspirate_delay = 500
sample_inject_speed = 1
calibrant_volume = 0.6
calibrantTime = 10
is_calibrant_inject = False
is_extended_wash = False
is_bottom_sense = True
injection_method = uLPickup
trap = Name: Thermo Trap Cartridge 5mm, Func: Separator, ColDia: 0.3 mm, Len: 5 mm, PartDia: 5 µm, Porosity: 42%
separator = Name: PepSep C18 25cm 150ID 1_5um, Func: Separator, ColDia: 0.15 mm, Len: 250 mm, PartDia: 1.5 µm, Porosity: 42%
separator_volume = 1.85550316102647
separator_unityflow = 0.00176496760821226
separator_equilibration_volumemultiplier = 4
separator_equilibration_pressure = 800
column_load_volumemultiplier = 2
column_load_pressure = 800
separator_equil_time = 5.64223885378452
column_load_time = 3.04081338813953
gradient = (0 sec,0.85 µL/min,2.00 %), (300 sec,0.85 µL/min,6.00 %), (1080 sec,0.85 µL/min,16.00 %), (1890 sec,0.85 µL/min,29.00 %), (2130 sec,0.85 µL/min,38.00 %), (2220 sec,0.85 µL/min,95.00 %), (2460 sec,0.85 µL/min,95.00 %)
00:00:00.0	STARTED
00:00:00.6	pump: GetSettings() --> PumpSettings
00:00:00.6	--------------------------------------------------------
00:00:00.6	--- Log instrument settings called from: settings
00:00:00.6	--------------------------------------------------------
00:00:00.6	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:00.6	--- PressureTrackingExcessFactor: 96
00:00:00.6	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:00.6	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:00.6	--- PC_OutputFilterDepth: 0
00:00:00.6	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:00.6	--- ExternalZeroPressureOffsetA: 125.087898254395; ExternalZeroPressureOffsetB: 128.337753295898
00:00:00.6	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:00.6	--------------------------------------------------------
00:00:00.6	Lua version:		1.1.0
00:00:00.6	Lua date:			2020/01/30
00:00:00.6	--- Experiment:    Description
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Trap Column:              Thermo Trap Cartridge 5mm
00:00:00.6	---    Manufacturer:          Thermo Scientific
00:00:00.6	---    Part Number:           
00:00:00.6	---    Serial Number:         
00:00:00.6	---    Type/Material:         µ-Precolum TM Cartridge  / PepMap TM C18
00:00:00.6	---    Length:                5 mm
00:00:00.6	---    Inner Diameter:        0.3 mm
00:00:00.6	---    Particle Diameter:     5 um
00:00:00.6	---    Is Particle Pore Size: True
00:00:00.6	---    Particle Pore Size:    100
00:00:00.6	---    Is Column Porosity:    False
00:00:00.6	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.6	---    Is Max Temperature:    False
00:00:00.6	---    max Temperature:       65 C
00:00:00.6	---    Is Max Pressure:       False
00:00:00.6	---    max Pressure:          1000 bar
00:00:00.6	---    Is Max Flow:           False
00:00:00.6	---    max Flow:              9 uL/min
00:00:00.6	---    Advanced Settings:     False
00:00:00.6	---    Column Comments:       Manufacturer no: 160454
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Separation Column:        PepSep C18 25cm 150ID 1_5um
00:00:00.6	---    Manufacturer:          Bruker Daltonics
00:00:00.6	---    Part Number:           1893476
00:00:00.6	---    Serial Number:         
00:00:00.6	---    Type/Material:         nano Separation Column / ReproSil C18 beads
00:00:00.6	---    Length:                250 mm
00:00:00.6	---    Inner Diameter:        0.15 mm
00:00:00.6	---    Particle Diameter:     1.5 um
00:00:00.6	---    Is Particle Pore Size: True
00:00:00.6	---    Particle Pore Size:    100
00:00:00.6	---    Is Column Porosity:    False
00:00:00.6	---    Column Porosity:       0.42 (0.0-1.0)
00:00:00.6	---    Is Max Temperature:    False
00:00:00.6	---    max Temperature:       65 C
00:00:00.6	---    Is Max Pressure:       False
00:00:00.6	---    max Pressure:          1000 bar
00:00:00.6	---    Is Max Flow:           False
00:00:00.6	---    max Flow:              9 uL/min
00:00:00.6	---    Advanced Settings:     False
00:00:00.6	---    Column Comments:           
00:00:00.6	-------------------------------------------------------
00:00:00.6	'Valve Drive 1' is 'ILcInjectorValve'
00:00:00.6	'Valve Drive 2' is 'ISelectorValve'
00:00:00.6	--- Seperator equil time:  5.64
00:00:00.6	--- Column loading time:   3.04
00:00:00.6	--- Sep.Length:                250
00:00:00.6	--- Sep.ColumnDiameter:        0.15
00:00:00.6	--- Sep.IsColumnPorosity:      False
00:00:00.6	--- Sep.ParticleDiameter:      1.5
00:00:00.6	--- oven_temperature:          40
00:00:00.6	--- Column viscoMix:           0.00661528865103582
00:00:00.6	--- Column flow @ 1020 bar:    1.76496760821226
00:00:00.6	--- Column unity flow:         0.00176496760821226
00:00:00.6	pump: GetSettings() --> PumpSettings
00:00:00.6	--------------------------------------------------------
00:00:00.6	--- Log instrument settings called from: Two Column Separation
00:00:00.6	--------------------------------------------------------
00:00:00.6	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:00.6	--- PressureTrackingExcessFactor: 96
00:00:00.6	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:00.6	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:00.6	--- PC_OutputFilterDepth: 0
00:00:00.6	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:00.6	--- ExternalZeroPressureOffsetA: 125.087898254395; ExternalZeroPressureOffsetB: 128.337753295898
00:00:00.6	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:00.6	--------------------------------------------------------
00:00:00.6	--- Oven temperature: 40
00:00:00.6	--- Separator: Name: PepSep C18 25cm 150ID 1_5um, Func: Separator, ColDia: 0.15 mm, Len: 250 mm, PartDia: 1.5 µm, Porosity: 42% ---
00:00:00.6	--- Trap:  ---
00:00:00.6	--- MaxPressureLimitAB: 1000 bar ---
00:00:00.6	pump: GetCurrentPressure(A) --> 4.255577
00:00:00.6	pump: GetCurrentPressure(B) --> 10.88544
00:00:00.6	pump: GetSettings() --> PumpSettings
00:00:00.6	pump: GetSetManualValvePosition(A) --> 240
00:00:00.6	pump: GetSetManualValvePosition(B) --> 240
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Reduce pressure ---
00:00:00.6	-------------------------------------------------------
00:00:00.6	--- Pressure channel A: 4.25557708740234
00:00:00.6	--- Pressure channel B: 10.8854417800903
00:00:00.6	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:00.6	pump: GetSetPistonSpeed(A) --> 0
00:00:00.6	pump: Manualmode_Pump_constantSpeed(B, 0)
00:00:00.6	pump: GetSetPistonSpeed(B) --> 0
00:00:00.6	pump: IsIdle() --> True
00:00:00.6	-------------------------------------------------------
00:00:00.7	pump: SetMaxPressureLimit(A, 1020)
00:00:00.7	pump: GetMaxPressureLimit(A) --> 1020
00:00:00.8	pump: SetMaxPressureLimit(B, 1020)
00:00:00.8	pump: GetMaxPressureLimit(B) --> 1020
00:00:00.8	pump: GetSettings() --> PumpSettings
00:00:00.8	------------------------------------------------------------------
00:00:00.8	PID settings changed:
00:00:00.8	------------------------------------------------------------------
00:00:00.8	P: 175 : True; Pmin: -5000 : True; Pmax: 5000 : True
00:00:00.8	I: 5 : True; Imin: -200 : True; Imax: 1000 : True; IWindow: 10 : True
00:00:00.8	D: 0 : True; Dmin: -1000 : True; Dmax: 1000 : True
00:00:00.8	Result min: -6000 : True; Result max: 8000 : True
00:00:00.8	------------------------------------------------------------------
00:00:00.8	pump: SetSettings(PumpSettings)
00:00:00.8	pump: GetPumpSideState(A) --> manualMode
00:00:00.8	pump: GetPumpSideState(B) --> manualMode
00:00:00.8	pump: GetPistonPosition(A) --> 585.677274751764
00:00:00.8	pump: GetPistonPosition(B) --> 407.854543538662
00:00:00.8	-------------------------------------------------------
00:00:00.8	--- Optional Refill of Pump Heads:
00:00:00.8	--- initiated by channel A:     False, B: False
00:00:00.8	pump: GetPistonPosition(A) --> 585.677274751764
00:00:00.8	pump: GetPistonPosition(B) --> 407.854543538662
00:00:00.8	--- Piston position channel A:  585.677274751764, B: 407.854543538662
00:00:00.8	-------------------------------------------------------
00:00:00.8	pump: SetDigitalOutput(PO2, False)
00:00:00.8	pump: GetCurrentPressure(A) --> 4.235857
00:00:00.8	pump: GetCurrentPressure(B) --> 10.90516
00:00:00.8	pump: GetSettings() --> PumpSettings
00:00:00.8	pump: GetSetManualValvePosition(A) --> 240
00:00:00.8	pump: GetSetManualValvePosition(B) --> 240
00:00:00.8	-------------------------------------------------------
00:00:00.8	--- Reduce pressure ---
00:00:00.8	-------------------------------------------------------
00:00:00.8	--- Pressure channel A: 4.2358570098877
00:00:00.8	--- Pressure channel B: 10.905161857605
00:00:00.8	pump: GetSettings() --> PumpSettings
00:00:00.8	------------------------------------------------------------------
00:00:00.8	PID settings changed:
00:00:00.8	------------------------------------------------------------------
00:00:00.8	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
00:00:00.8	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
00:00:00.8	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:00.8	Result min: -32000 : True; Result max: 32000 : True
00:00:00.8	------------------------------------------------------------------
00:00:00.8	pump: SetSettings(PumpSettings)
00:00:01.1	--- Set pressure channel A:          10
00:00:01.1	--- Set pressure channel B:          5
00:00:01.1	--- Reduce pressure flow controlled: True
00:00:02.3	--------------------------------------------------------
00:00:02.3	--- Log PID parameter called from: reducePressure
00:00:02.3	--------------------------------------------------------
00:00:02.3	--- P: 175; Pmin: -5000; Pmax: 5000
00:00:02.3	--- I: 5; Imin: -200; Imax: 1000; IWindow: 10
00:00:02.3	--- D: 0; Dmin: -1000; Dmax: 1000
00:00:02.3	--- Result min: -6000; Result max: 8000
00:00:02.3	--------------------------------------------------------
00:00:02.3	--------------------------------------------------------
00:00:02.3	--- Log instrument settings called from: reducePressure
00:00:02.3	--------------------------------------------------------
00:00:02.3	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:00:02.3	--- PressureTrackingExcessFactor: 96
00:00:02.3	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:00:02.3	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:00:02.3	--- PC_OutputFilterDepth: 0
00:00:02.3	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:00:02.3	--- ExternalZeroPressureOffsetA: 125.087898254395; ExternalZeroPressureOffsetB: 128.337753295898
00:00:02.3	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:00:02.3	--------------------------------------------------------
00:00:02.3	--- Final pressure channel A: 4.35417699813843
00:00:02.3	--- Final pressure channel B: 0
00:00:02.3	-------------------------------------------------------
00:00:02.3	pump: GetSetManualValvePosition(A) --> 240
00:00:02.3	pump: GetCurrentPressure(A) --> 4.354177
00:00:02.3	pump: SetManualValvePosition(A, 0, True)
00:00:03.1	pump: GetSetManualValvePosition(B) --> 240
00:00:03.1	pump: GetCurrentPressure(B) --> 1.045162
00:00:03.1	pump: SetManualValvePosition(B, 180, False)
00:00:03.5	pump: GetSettings() --> PumpSettings
00:00:03.5	------------------------------------------------------------------
00:00:03.5	PID settings changed:
00:00:03.5	------------------------------------------------------------------
00:00:03.5	P: 250 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:00:03.5	I: 20 : True; Imin: -10000 : True; Imax: 20000 : True; IWindow: 10 : True
00:00:03.5	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:00:03.5	Result min: -10000 : True; Result max: 32000 : True
00:00:03.5	------------------------------------------------------------------
00:00:03.5	pump: SetSettings(PumpSettings)
00:00:03.5	status: SetStatus(equilibrate separation-column, this finishes at about: 6.60 min., )
00:00:03.7	autosampler-none: MoveValveDrive(Valve Drive 2, 60 deg)
00:00:03.8	pump: Manualmode_Pump_constantPressure(A, 214)
00:00:03.8	pump: GetTargetPressure(A) --> 214
00:00:03.8	--- Injection delay: 293.534331227071s
00:00:34.0	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:34.0	pump: GetSetPistonSpeed(A) --> 0
00:00:34.1	autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg)
00:00:34.1	--- MaxPressureLimitA: 800 bar ---
00:00:34.1	pump: GetCurrentPressure(A) --> 213.3073
00:00:34.1	pump: GetCurrentPressure() --> 213.3073
00:00:34.1	pump: GetSettings() --> PumpSettings
00:00:34.1	pump: GetSetManualValvePosition(A) --> 0
00:00:34.1	-------------------------------------------------------
00:00:34.1	--- Reduce pressure ---
00:00:34.1	-------------------------------------------------------
00:00:34.1	--- Pressure channel A: 213.30729675293
00:00:34.1	--- Pressure channel B: 213.30729675293
00:00:34.1	pump: Manualmode_Pump_constantSpeed(A, 0)
00:00:34.1	pump: GetSetPistonSpeed(A) --> 0
00:00:34.1	pump: Manualmode_Pump_constantSpeed(, 0)
00:00:34.1	pump: GetSetPistonSpeed() --> 0
00:00:34.1	pump: IsIdle() --> True
00:00:34.1	-------------------------------------------------------
00:00:34.3	pump: SetMaxPressureLimit(A, 820)
00:00:34.3	pump: GetMaxPressureLimit(A) --> 820
00:00:34.3	-------------------------------------------------------
00:00:34.3	--- Separation Column Equilibration:
00:00:34.3	-------------------------------------------------------
00:00:34.3	--- ovenTemp [C]:                                 40
00:00:34.3	--- Separator Unity-Flow @ ovenTemp [ul/min/bar]: 0.00176496760821226
00:00:34.3	--- Separator Volume [ul]:                        1.85550316102647
00:00:34.3	--- Separator Equilibration Volume Multiplier:    4
00:00:34.3	--- Separator Equilibration Pressure [bar]:       800
00:00:34.3	--- Separator Equilibration Flow [ul/min]:        1.31543751273975
00:00:34.3	--- Separator Equilibration Volume [ul]:          7.42201264410588
00:00:34.3	--- Separator Equilibration Time [min]:           5.64223885378452
00:00:34.3	--- max. Volume (10*V+2ul/min*t) [ul]:            85.5046041486279
00:00:34.3	--- Separator Equilibration Time Out [min]:       56.4223885378452
00:00:34.3	-------------------------------------------------------
00:00:34.3	pump: Manualmode_Pump_constantPressure(A, 800)
00:00:34.3	pump: GetPistonPosition(A) --> 610.103157633425
00:00:50.3	pump: GetPistonPosition(A) --> 660.152917395315
00:00:50.3	pump: GetPistonPosition(A) --> 660.152917395315
00:00:50.3	pump: GetPistonPosition(A) --> 660.152917395315
00:04:57.5	status: SetStatus(aspirate & inject, )
00:04:57.7	autosampler-left: LeaveObject()
00:05:01.9	autosampler-left: MoveToObject(LCMS Wash 1, 3)
00:05:02.6	autosampler-left: PenetrateObject(LCMS Wash 1, 3)
00:05:02.7	autosampler-left: EmptySyringe()
00:05:03.4	autosampler-left: LeaveObject()
00:05:03.5	Cleaning syringe and needle exterior
00:05:19.7	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:05:19.7	autosampler-left: ChangeTool(LCP 1) --> False
00:05:22.8	autosampler-left: MoveToObject(Item Position 3, 1, True, True, True)
00:05:26.1	autosampler-left: PenetrateWithBottomSense(Item Position 3, 1, 1 mm)
00:05:27.1	autosampler-left: AspirateSyringe(2 uL, 5 uL/s, 500 ms)
00:05:27.9	autosampler-left: LeaveObject()
00:05:28.7	autosampler-left: AspirateSyringe(0.5 uL, 5 uL/s, 500 ms)
00:05:38.7	autosampler-left: MoveToObject(Rack 2, 37, True, True, True)
00:05:42.2	autosampler-left: PenetrateWithBottomSense(Rack 2, 37, 1 mm)
00:05:43.0	autosampler-left: AspirateSyringe(1.25 uL, 5 uL/s, 500 ms)
00:05:50.5	autosampler-left: LeaveObject()
00:05:51.5	autosampler-left: AspirateSyringe(1 uL, 5 uL/s, 500 ms)
00:05:54.9	autosampler-left: MoveToObject(LCMS Wash 1, 1, True, True, True)
00:05:55.9	autosampler-left: PenetrateObject(LCMS Wash 1, 1, 30 mm)
00:06:00.9	autosampler-left: Wait(5 s)
00:06:01.7	autosampler-left: LeaveObject()
00:06:04.7	autosampler-left: MoveToObject(LCInjector1)
00:06:05.6	autosampler-left: DispenseSyringe(0.75 uL, 1 uL/s)
00:06:08.0	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:06:08.5	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:06:09.5	autosampler-left: Wait(1 s)
00:06:12.6	autosampler-left: DispenseSyringe(3 uL, 1 uL/s)
00:06:13.6	autosampler-left: Wait(1 s)
00:06:14.2	status: RemoveStatus(aspirate & inject)
00:06:29.2	pump: GetPistonPosition(A) --> 669.158188017075
00:06:29.2	pump: GetCurrentPressure(A) --> 799.9773
00:06:29.2	--- act. pressure [bar]:                 799.977294921875
00:06:29.2	--- used volume [uL]:                    9.00527062176013
00:06:29.2	--- average flow (incl. leaks) [uL/min]: 1.59409772975692
00:06:29.2	--- act. equilibration time [min]:       5.64913333333291
00:06:29.2	-------------------------------------------------------
00:06:29.2	status: RemoveStatus(equilibrate separation-column, this finishes at about: 6.60 min.)
00:06:31.3	autosampler-none: MoveValveDrive(Valve Drive 2, 180 deg)
00:06:31.3	pump: GetSetManualValvePosition(A) --> 0
00:06:31.3	pump: GetCurrentPressure(A) --> 800.0167
00:06:31.3	pump: SetManualValvePosition(A, 0, False)
00:06:31.5	status: SetStatus(load separation-column, this finishes at about: 10.02 min., )
00:06:31.7	autosampler-none: MoveValveDrive(Valve Drive 1, 60 deg)
00:06:33.3	autosampler-left: EmptySyringe(5 uL/s)
00:06:33.3	-------------------------------------------------------
00:06:33.3	--- Sample Loading on Separation Column:
00:06:33.3	-------------------------------------------------------
00:06:33.3	--- ovenTemp [C]:                      40
00:06:33.3	--- Separator Unity-Flow [ul/bar]:     0.00176496760821226
00:06:33.3	--- Separator Volume [ul]:             1.85550316102647
00:06:33.3	--- Sample Volume [ul]:                1.25
00:06:33.3	--- Separator Load Pressure [bar]:     800
00:06:33.3	--- Separator Load Flow [ul/min]:      1.31543751273975
00:06:33.3	--- Separator Volume Multiplier:       2
00:06:33.3	--- Separator Load Volume (+2) [ul]:   4.5
00:06:33.3	--- Separator Load Time [min]:         3.42091506165698
00:06:33.3	--- max. Volume (10*V+2ul/min*t) [ul]: 51.8418301233139
00:06:33.3	--- Separator Load Time Out [min]:     34.2091506165698
00:06:33.3	-------------------------------------------------------
00:06:33.3	pump: Manualmode_Pump_constantPressure(A, 800)
00:06:33.3	pump: GetPistonPosition(A) --> 669.648904789565
00:06:37.1	pump: GetPistonPosition(A) --> 670.67683390582
00:06:37.1	pump: GetPistonPosition(A) --> 670.67683390582
00:06:37.1	pump: GetPistonPosition(A) --> 670.67683390582
00:07:32.2	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1 s, 2, False)
00:08:33.3	autosampler-left: CleanInjector(LCInjector1, LCP 1, 200 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1 s, 1, True)
00:08:49.4	autosampler-left: CleanSyringe(LCMS Wash 1, 2, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 2)
00:09:09.5	autosampler-left: CleanSyringe(LCMS Wash 1, 1, 30 mm, 40 uL/s, 500 ms, 30 mm, 40 uL/s, 80 %, 3)
00:09:12.7	autosampler-left: MoveToHome()
00:10:02.5	pump: GetPistonPosition(A) --> 675.860461784243
00:10:02.5	pump: GetCurrentPressure(A) --> 799.9773
00:10:02.5	-------------------------------------------------------
00:10:02.5	--- Finished: Sample Loading on Separation Column: (const_pressure_load_and_equilibration)
00:10:02.5	-------------------------------------------------------
00:10:02.5	--- act. pressure [bar]:                 799.977294921875
00:10:02.5	--- used volume [uL]:                    5.18362787842329
00:10:02.5	--- average flow (incl. leaks) [uL/min]: 1.51407951973485
00:10:02.5	--- act. loading time [min]:             3.42361666666693
00:10:02.5	-------------------------------------------------------
00:10:02.5	status: RemoveStatus(load separation-column, this finishes at about: 10.02 min.)
00:10:02.5	pump: GetSettings() --> PumpSettings
00:10:02.5	------------------------------------------------------------------
00:10:02.5	PID settings changed:
00:10:02.5	------------------------------------------------------------------
00:10:02.5	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:10:02.5	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:10:02.5	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:10:02.5	Result min: -5000 : True; Result max: 25000 : True
00:10:02.5	------------------------------------------------------------------
00:10:02.5	pump: SetSettings(PumpSettings)
00:10:02.5	pump: GetSettings() --> PumpSettings
00:10:02.5	------------------------------------------------------------------
00:10:02.5	PID settings changed:
00:10:02.5	------------------------------------------------------------------
00:10:02.5	P: 100 : False; Pmin: -10000 : False; Pmax: 10000 : False
00:10:02.5	I: 100 : False; Imin: -1000 : False; Imax: 1000 : False; IWindow: 2 : True
00:10:02.5	D: 5000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:10:02.5	Result min: -5000 : False; Result max: 25000 : False
00:10:02.5	------------------------------------------------------------------
00:10:02.5	pump: SetSettings(PumpSettings)
00:10:02.5	status: SetStatus(preparing gradient start, )
00:10:02.5	--- Starting conditioning paths ---
00:10:02.5	pump: GetSettings() --> PumpSettings
00:10:02.5	------------------------------------------------------------------
00:10:02.5	PID settings changed:
00:10:02.5	------------------------------------------------------------------
00:10:02.5	P: 100 : True; Pmin: -10000 : True; Pmax: 10000 : True
00:10:02.5	I: 100 : True; Imin: -1000 : True; Imax: 1000 : True; IWindow: 100 : True
00:10:02.5	D: 5000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:10:02.5	Result min: -5000 : True; Result max: 25000 : True
00:10:02.5	------------------------------------------------------------------
00:10:02.5	pump: SetSettings(PumpSettings)
00:10:02.6	pump: Manualmode_Pump_constantPressure(A, 0)
00:10:02.6	pump: GetTargetPressure(A) --> 0
00:10:02.6	pump: GetCurrentPressure(A) --> 800.0759
00:10:03.6	pump: GetCurrentPressure(A) --> 762.6671
00:10:04.6	pump: GetCurrentPressure(A) --> 710.8823
00:10:05.6	pump: GetCurrentPressure(A) --> 660.8527
00:10:06.6	pump: GetCurrentPressure(A) --> 609.5807
00:10:07.6	pump: GetCurrentPressure(A) --> 564.6191
00:10:08.6	pump: GetCurrentPressure(A) --> 515.5754
00:10:09.6	pump: GetCurrentPressure(A) --> 466.7881
00:10:10.6	pump: GetCurrentPressure(A) --> 418.5333
00:10:11.6	pump: GetCurrentPressure(A) --> 370.6335
00:10:12.6	pump: GetCurrentPressure(A) --> 328.2946
00:10:13.6	pump: GetCurrentPressure(A) --> 282.0512
00:10:14.6	pump: GetCurrentPressure(A) --> 236.636
00:10:15.6	pump: GetCurrentPressure(A) --> 192.1083
00:10:16.6	pump: GetCurrentPressure(A) --> 147.8566
00:10:17.6	pump: GetCurrentPressure(A) --> 108.18
00:10:18.6	pump: GetCurrentPressure(A) --> 65.1115
00:10:19.6	pump: GetCurrentPressure(A) --> 33.73698
00:10:20.6	pump: GetCurrentPressure(A) --> 18.51314
00:10:21.6	pump: GetCurrentPressure(A) --> 10.70402
00:10:21.8	autosampler-none: MoveValveDrive(Valve Drive 2, 0 deg)
00:10:21.8	pump: SetManualValvePosition(A, 240, False)
00:10:22.6	pump: SetManualValvePosition(B, 240, False)
00:10:24.6	--- Set constant binary flow ---
00:10:24.6	pump: Manualmode_Pump_constantFlow_binary(1, 1)
00:10:24.6	pump: GetSetFlow(A) --> 1
00:10:24.6	pump: GetSetFlow(B) --> 1
00:10:24.6	

00:10:24.6	--- Starting spline flow reduction B/A ---
00:10:24.7	pump: GetSetExternalTemperature() --> 40
00:10:24.7	--- Oven temperatur: 40
00:10:24.7	--- Separator: Name: PepSep C18 25cm 150ID 1_5um, Func: Separator, ColDia: 0.15 mm, Len: 250 mm, PartDia: 1.5 µm, Porosity: 42%
00:10:24.7	--- Spline fitting time: 69.1645659011966 s
00:10:24.7	--- Spline fitting time adapted: 10 s
00:10:24.7	--- y = 0x + 1
00:10:24.7	--- p0.Time -1, p0.Flow 1
00:10:24.7	--- p1.Time 0, p1.Flow 1
00:10:24.7	--- p2.Time 9, p2.Flow 1
00:10:24.7	--- p3.Time 10, p3.Flow 1, p3.Mix 0
00:10:24.7	--- p4.Time 40, p4.Flow 1, p4.Mix 0
00:10:24.7	--- Strategy function end
00:10:24.7	--- y = 0x + 0
00:10:24.7	--- p0.Time -1, p0.Flow 1
00:10:24.7	--- p1.Time 0, p1.Flow 1
00:10:24.7	--- p2.Time 9, p2.Flow 0
00:10:24.7	--- p3.Time 10, p3.Flow 0, p3.Mix 0
00:10:24.7	--- p4.Time 40, p4.Flow 0, p4.Mix 0
00:10:24.7	--- Strategy function end
00:10:24.7	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:10:24.7	XXXXX Time 0, Flow 2, Mix 50
00:10:24.7	XXXXX Time 1, Flow 1.94545, Mix 48.59813
00:10:24.7	XXXXX Time 2, Flow 1.84848, Mix 45.90163
00:10:24.7	XXXXX Time 3, Flow 1.72121, Mix 41.9014
00:10:24.7	XXXXX Time 4, Flow 1.57575, Mix 36.53846
00:10:24.7	XXXXX Time 5, Flow 1.42424, Mix 29.78723
00:10:24.7	XXXXX Time 6, Flow 1.27878, Mix 21.80094
00:10:24.7	XXXXX Time 7, Flow 1.15151, Mix 13.15789
00:10:24.7	XXXXX Time 8, Flow 1.05454, Mix 5.17241
00:10:24.7	XXXXX Time 9, Flow 1, Mix 0
00:10:24.7	--- Gradient is created, switching valves A and B
00:10:24.7	pump: GetSetManualValvePosition(A) --> 240
00:10:24.7	pump: GetCurrentPressure(A) --> 3.703417
00:10:24.7	pump: SetManualValvePosition(A, 240, False)
00:10:24.9	pump: GetSetManualValvePosition(B) --> 240
00:10:24.9	pump: GetCurrentPressure(B) --> 1.143762
00:10:24.9	pump: SetManualValvePosition(B, 240, False)
00:10:25.1	--- Valves A and B switched to MixTee, starting gradient
00:10:25.1	--- LoadGradient() ---
00:10:25.1	--- Loading gradient method index: 0
00:10:25.1	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:10:25.6	pump: GetMethodState(0) --> loaded
00:10:25.6	--- Set gradient start
00:10:25.6	--- StartGradient() ---
00:10:25.6	--- Starting gradient method index: 0
00:10:25.6	pump: SetMethodSignal(0, StartTrunk)
00:10:25.8	pump: GetMethodState(0) --> runningTrunk
00:10:25.8	--- Start GradientSplineFit
00:10:25.8	pump: GetMethodState(0) --> runningTrunk
00:10:26.8	pump: GetCurrentPressure(A) --> 79.84233
00:10:26.8	pump: GetCurrentPressure(B) --> 47.9196
00:10:26.8	--- GradientSplineFit is running
00:10:34.8	--- GradientSplineFit has finished
00:10:34.8	--- End GradientSplineFit
00:10:34.8	--- Finished spline flow reduction B ---
00:10:35.9	pump: GetSetExternalTemperature() --> 40
00:10:35.9	--- Oven temperatur: 40
00:10:35.9	--- Separator: Name: PepSep C18 25cm 150ID 1_5um, Func: Separator, ColDia: 0.15 mm, Len: 250 mm, PartDia: 1.5 µm, Porosity: 42%
00:10:35.9	--- Spline fitting time: 69.1645659011966 s
00:10:35.9	--- Spline fitting time adapted: 60 s
00:10:35.9	--- y = 0x + 0.01
00:10:35.9	--- p0.Time -1, p0.Flow 1
00:10:35.9	--- p1.Time 0, p1.Flow 1
00:10:35.9	--- p2.Time 59, p2.Flow 0.01
00:10:35.9	--- p3.Time 60, p3.Flow 0.01, p3.Mix 0
00:10:35.9	--- p4.Time 90, p4.Flow 0.01, p4.Mix 0
00:10:35.9	--- Strategy function end
00:10:35.9	--- y = 0x + 0
00:10:35.9	--- p0.Time -1, p0.Flow 0
00:10:35.9	--- p1.Time 0, p1.Flow 0
00:10:35.9	--- p2.Time 59, p2.Flow 0
00:10:35.9	--- p3.Time 60, p3.Flow 0, p3.Mix 0
00:10:35.9	--- p4.Time 90, p4.Flow 0, p4.Mix 0
00:10:35.9	--- Strategy function end
00:10:35.9	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:10:35.9	XXXXX Time 0, Flow 1, Mix 0
00:10:35.9	XXXXX Time 1, Flow 0.99837, Mix 0
00:10:35.9	XXXXX Time 2, Flow 0.99518, Mix 0
00:10:35.9	XXXXX Time 3, Flow 0.99048, Mix 0
00:10:35.9	XXXXX Time 4, Flow 0.98432, Mix 0
00:10:35.9	XXXXX Time 5, Flow 0.97675, Mix 0
00:10:35.9	XXXXX Time 6, Flow 0.96784, Mix 0
00:10:35.9	XXXXX Time 7, Flow 0.95763, Mix 0
00:10:35.9	XXXXX Time 8, Flow 0.94619, Mix 0
00:10:35.9	XXXXX Time 9, Flow 0.93356, Mix 0
00:10:35.9	XXXXX Time 10, Flow 0.91981, Mix 0
00:10:35.9	XXXXX Time 11, Flow 0.90498, Mix 0
00:10:35.9	XXXXX Time 12, Flow 0.88914, Mix 0
00:10:35.9	XXXXX Time 13, Flow 0.87233, Mix 0
00:10:35.9	XXXXX Time 14, Flow 0.85462, Mix 0
00:10:35.9	XXXXX Time 15, Flow 0.83605, Mix 0
00:10:35.9	XXXXX Time 16, Flow 0.81668, Mix 0
00:10:35.9	XXXXX Time 17, Flow 0.79658, Mix 0
00:10:35.9	XXXXX Time 18, Flow 0.77578, Mix 0
00:10:35.9	XXXXX Time 19, Flow 0.75435, Mix 0
00:10:35.9	XXXXX Time 20, Flow 0.73235, Mix 0
00:10:35.9	XXXXX Time 21, Flow 0.70982, Mix 0
00:10:35.9	XXXXX Time 22, Flow 0.68682, Mix 0
00:10:35.9	XXXXX Time 23, Flow 0.66341, Mix 0
00:10:35.9	XXXXX Time 24, Flow 0.63964, Mix 0
00:10:35.9	XXXXX Time 25, Flow 0.61558, Mix 0
00:10:35.9	XXXXX Time 26, Flow 0.59126, Mix 0
00:10:35.9	XXXXX Time 27, Flow 0.56675, Mix 0
00:10:35.9	XXXXX Time 28, Flow 0.5421, Mix 0
00:10:35.9	XXXXX Time 29, Flow 0.51737, Mix 0
00:10:35.9	XXXXX Time 30, Flow 0.49262, Mix 0
00:10:35.9	XXXXX Time 31, Flow 0.46789, Mix 0
00:10:35.9	XXXXX Time 32, Flow 0.44324, Mix 0
00:10:35.9	XXXXX Time 33, Flow 0.41873, Mix 0
00:10:35.9	XXXXX Time 34, Flow 0.39441, Mix 0
00:10:35.9	XXXXX Time 35, Flow 0.37035, Mix 0
00:10:35.9	XXXXX Time 36, Flow 0.34658, Mix 0
00:10:35.9	XXXXX Time 37, Flow 0.32317, Mix 0
00:10:35.9	XXXXX Time 38, Flow 0.30017, Mix 0
00:10:35.9	XXXXX Time 39, Flow 0.27764, Mix 0
00:10:35.9	XXXXX Time 40, Flow 0.25564, Mix 0
00:10:35.9	XXXXX Time 41, Flow 0.23421, Mix 0
00:10:35.9	XXXXX Time 42, Flow 0.21341, Mix 0
00:10:35.9	XXXXX Time 43, Flow 0.19331, Mix 0
00:10:35.9	XXXXX Time 44, Flow 0.17394, Mix 0
00:10:35.9	XXXXX Time 45, Flow 0.15537, Mix 0
00:10:35.9	XXXXX Time 46, Flow 0.13766, Mix 0
00:10:35.9	XXXXX Time 47, Flow 0.12085, Mix 0
00:10:35.9	XXXXX Time 48, Flow 0.10501, Mix 0
00:10:35.9	XXXXX Time 49, Flow 0.09018, Mix 0
00:10:35.9	XXXXX Time 50, Flow 0.07643, Mix 0
00:10:35.9	XXXXX Time 51, Flow 0.0638, Mix 0
00:10:35.9	XXXXX Time 52, Flow 0.05236, Mix 0
00:10:35.9	XXXXX Time 53, Flow 0.04215, Mix 0
00:10:35.9	XXXXX Time 54, Flow 0.03324, Mix 0
00:10:35.9	XXXXX Time 55, Flow 0.02567, Mix 0
00:10:35.9	XXXXX Time 56, Flow 0.01951, Mix 0
00:10:35.9	XXXXX Time 57, Flow 0.01481, Mix 0
00:10:35.9	XXXXX Time 58, Flow 0.01162, Mix 0
00:10:35.9	XXXXX Time 59, Flow 0.00999, Mix 0
00:10:35.9	--- Gradient is created, switching valves A and B
00:10:35.9	pump: GetSetManualValvePosition(A) --> 240
00:10:35.9	pump: GetCurrentPressure(A) --> 85.4231
00:10:35.9	pump: SetManualValvePosition(A, 240, False)
00:10:36.1	pump: GetSetManualValvePosition(B) --> 240
00:10:36.1	pump: GetCurrentPressure(B) --> 12.95604
00:10:36.1	pump: SetManualValvePosition(B, 240, False)
00:10:36.3	--- Valves A and B switched to MixTee, starting gradient
00:10:36.3	--- LoadGradient() ---
00:10:36.3	--- Loading gradient method index: 0
00:10:36.3	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:10:36.8	pump: GetMethodState(0) --> loaded
00:10:36.8	--- Set gradient start
00:10:36.8	--- StartGradient() ---
00:10:36.8	--- Starting gradient method index: 0
00:10:36.8	pump: SetMethodSignal(0, StartTrunk)
00:10:37.0	pump: GetMethodState(0) --> runningTrunk
00:10:37.0	--- Start GradientSplineFit
00:10:37.0	pump: GetMethodState(0) --> runningTrunk
00:10:38.0	pump: GetCurrentPressure(A) --> 86.66545
00:10:38.0	pump: GetCurrentPressure(B) --> 14.71112
00:10:38.0	--- GradientSplineFit is running
00:11:36.1	--- GradientSplineFit has finished
00:11:36.1	--- End GradientSplineFit
00:11:36.1	--- Finished spline flow reduction A ---

00:11:36.1	--- Finished conditioning paths ---
00:11:41.3	autosampler-none: MoveValveDrive(Valve Drive 2, 120 deg)
00:11:41.3	pump: GetSetExternalTemperature() --> 40
00:11:41.3	--- Oven temperatur: 40
00:11:41.3	--- Separator: Name: PepSep C18 25cm 150ID 1_5um, Func: Separator, ColDia: 0.15 mm, Len: 250 mm, PartDia: 1.5 µm, Porosity: 42%
00:11:41.3	--- Spline fitting time: 69.1645659011966 s
00:11:41.3	--- Spline fitting time adapted: 74.9052248709959 s
00:11:41.3	--- Press: 570.8142439303; colPress: 485.8142439303, visco: 0.00667324172495603, flow > 0.85
00:11:41.3	--- Flow: 0.85; Mix: 0.02, calcI: 200.43002924879
00:11:41.3	--- Ki calc: 200.43002924879
00:11:41.3	pump: GetSettings() --> PumpSettings
00:11:41.3	------------------------------------------------------------------
00:11:41.3	PID settings changed:
00:11:41.3	------------------------------------------------------------------
00:11:41.3	P: 200 : True; Pmin: -32000 : True; Pmax: 32000 : True
00:11:41.3	I: 600 : True; Imin: -32000 : True; Imax: 32000 : True; IWindow: 100 : True
00:11:41.3	D: 1000 : True; Dmin: -32000 : True; Dmax: 32000 : True
00:11:41.3	Result min: -32000 : True; Result max: 32000 : True
00:11:41.3	------------------------------------------------------------------
00:11:41.3	pump: SetSettings(PumpSettings)
00:11:41.3	pump: GetSettings() --> PumpSettings
00:11:41.3	------------------------------------------------------------------
00:11:41.3	PID settings changed:
00:11:41.3	------------------------------------------------------------------
00:11:41.3	P: 200 : False; Pmin: -32000 : False; Pmax: 32000 : False
00:11:41.3	I: 200 : True; Imin: -32000 : False; Imax: 32000 : False; IWindow: 100 : False
00:11:41.3	D: 1000 : False; Dmin: -32000 : False; Dmax: 32000 : False
00:11:41.3	Result min: -32000 : False; Result max: 32000 : False
00:11:41.3	------------------------------------------------------------------
00:11:41.4	pump: SetSettings(PumpSettings)
00:11:41.4	--- y = -0.000113333333333333x + 0.841489258818713
00:11:41.4	--- p0.Time -1, p0.Flow 0.01
00:11:41.4	--- p1.Time 0, p1.Flow 0.01
00:11:41.4	--- p2.Time 73.9052248709959, p2.Flow 0.833113333333333
00:11:41.4	--- p3.Time 74.9052248709959, p3.Flow 0.833, p3.Mix 2
00:11:41.4	--- p4.Time 374.905224870996, p4.Flow 0.799, p4.Mix 6
00:11:41.4	--- Strategy function end
00:11:41.4	--- y = 0.000113333333333333x + 0.00851074118128713
00:11:41.4	--- p0.Time -1, p0.Flow 0
00:11:41.4	--- p1.Time 0, p1.Flow 0
00:11:41.4	--- p2.Time 73.9052248709959, p2.Flow 0.0168866666666667
00:11:41.4	--- p3.Time 74.9052248709959, p3.Flow 0.017, p3.Mix 2
00:11:41.4	--- p4.Time 374.905224870996, p4.Flow 0.051, p4.Mix 6
00:11:41.4	--- Strategy function end
00:11:41.4	xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
00:11:41.4	XXXXX Time 0, Flow 0.01, Mix 0
00:11:41.4	XXXXX Time 1, Flow 0.01088, Mix 0.13699
00:11:41.4	XXXXX Time 2, Flow 0.01263, Mix 0.35122
00:11:41.4	XXXXX Time 3, Flow 0.01522, Mix 0.57829
00:11:41.4	XXXXX Time 4, Flow 0.01862, Mix 0.78128
00:11:41.4	XXXXX Time 5, Flow 0.02281, Mix 0.94865
00:11:41.4	XXXXX Time 6, Flow 0.02777, Mix 1.08187
00:11:41.4	XXXXX Time 7, Flow 0.03347, Mix 1.18671
00:11:41.4	XXXXX Time 8, Flow 0.03989, Mix 1.26935
00:11:41.4	XXXXX Time 9, Flow 0.04701, Mix 1.335
00:11:41.4	XXXXX Time 10, Flow 0.05479, Mix 1.38772
00:11:41.4	XXXXX Time 11, Flow 0.06322, Mix 1.43058
00:11:41.4	XXXXX Time 12, Flow 0.07228, Mix 1.46587
00:11:41.4	XXXXX Time 13, Flow 0.08193, Mix 1.49528
00:11:41.4	XXXXX Time 14, Flow 0.09216, Mix 1.52009
00:11:41.4	XXXXX Time 15, Flow 0.10293, Mix 1.54127
00:11:41.4	XXXXX Time 16, Flow 0.11424, Mix 1.55953
00:11:41.4	XXXXX Time 17, Flow 0.12604, Mix 1.57546
00:11:41.4	XXXXX Time 18, Flow 0.13833, Mix 1.58949
00:11:41.4	XXXXX Time 19, Flow 0.15107, Mix 1.60196
00:11:41.4	XXXXX Time 28, Flow 0.28223, Mix 1.67583
00:11:41.4	XXXXX Time 37, Flow 0.43079, Mix 1.72356
00:11:41.4	XXXXX Time 46, Flow 0.57926, Mix 1.768
00:11:41.4	XXXXX Time 55, Flow 0.71015, Mix 1.81857
00:11:41.4	XXXXX Time 64, Flow 0.80597, Mix 1.88346
00:11:41.4	XXXXX Time 65, Flow 0.81369, Mix 1.89202
00:11:41.4	XXXXX Time 66, Flow 0.82074, Mix 1.90092
00:11:41.4	XXXXX Time 67, Flow 0.82709, Mix 1.91019
00:11:41.4	XXXXX Time 68, Flow 0.83272, Mix 1.91985
00:11:41.4	XXXXX Time 69, Flow 0.83761, Mix 1.92993
00:11:41.4	XXXXX Time 70, Flow 0.84173, Mix 1.94047
00:11:41.4	XXXXX Time 71, Flow 0.84505, Mix 1.95149
00:11:41.4	XXXXX Time 72, Flow 0.84756, Mix 1.96304
00:11:41.4	XXXXX Time 73, Flow 0.84923, Mix 1.97516
00:11:41.4	XXXXX Time 74, Flow 0.85003, Mix 1.9879
00:11:41.4	--- Gradient is created, switching valves A and B
00:11:41.4	pump: GetSetManualValvePosition(A) --> 240
00:11:41.4	pump: GetCurrentPressure(A) --> 0.5482172
00:11:41.4	pump: SetManualValvePosition(A, 240, False)
00:11:41.6	pump: GetSetManualValvePosition(B) --> 240
00:11:41.6	pump: GetCurrentPressure(B) --> 0.433842
00:11:41.6	pump: SetManualValvePosition(B, 240, False)
00:11:41.8	--- Valves A and B switched to MixTee, starting gradient
00:11:41.8	--- LoadGradient() ---
00:11:41.8	--- Loading gradient method index: 0
00:11:41.8	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:11:42.3	pump: GetMethodState(0) --> loaded
00:11:42.3	--- Set gradient start
00:11:42.3	--- StartGradient() ---
00:11:42.3	--- Starting gradient method index: 0
00:11:42.3	pump: SetMethodSignal(0, StartTrunk)
00:11:42.5	pump: GetMethodState(0) --> runningTrunk
00:11:42.5	--- Start GradientSplineFit
00:11:42.5	pump: GetMethodState(0) --> runningTrunk
00:11:43.5	pump: GetCurrentPressure(A) --> 4.788017
00:11:43.5	pump: GetCurrentPressure(B) --> 4.160922
00:11:43.5	--- GradientSplineFit is running
00:12:56.6	--- GradientSplineFit has finished
00:12:56.6	--- End GradientSplineFit
00:12:56.6	status: RemoveStatus(preparing gradient start)
00:12:56.6	Setting signal "lc-ready"
00:12:56.6	Waiting for signal "ms-start"...
00:12:56.6	--- LoadGradient() ---
00:12:56.6	--- Loading gradient method index: 0
00:12:56.6	pump: LoadMethod(0, ZrData.ZrPumpMethod)
00:12:57.1	pump: GetMethodState(0) --> loaded
00:12:57.1	--- Set gradient start
00:12:57.1	--- StartGradient() ---
00:12:57.1	--- Starting gradient method index: 0
00:12:57.1	pump: SetMethodSignal(0, StartTrunk)
00:12:57.3	pump: GetMethodState(0) --> runningTrunk
00:12:57.4	autosampler-none: SetSignal(Output Signal 1)
00:12:57.4	status: SetStatus(wash, )
00:13:00.0	status: SetStatus(elution, )
00:13:01.9	autosampler-left: MoveToObject(LCInjector1)
00:13:04.5	autosampler-left: PenetrateWithConstForce(LCInjector1)
00:13:04.6	autosampler-left: MoveValveDrive(Valve Drive 1, 0 deg)
00:13:34.6	autosampler-left: CleanInjector(LCInjector1, LCP 1, 100 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1 s, 2, False)
00:15:04.5	autosampler-left: CleanInjector(LCInjector1, LCP 1, 300 uL, 0 mm, 5 uL/s, 5 s, 20 mm, 40 uL/s, 1 s, 1, True)
00:15:04.6	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:15:08.0	autosampler-left: MoveToHome()
00:53:57.4	Setting signal "lc-done"
00:53:57.4	status: RemoveStatus(elution)
00:53:57.4	status: RemoveStatus(wash)
00:53:57.4	pump: GetCurrentPressure(A) --> 235.157
00:53:57.4	pump: GetCurrentPressure(B) --> 257.8193
00:53:57.4	pump: GetSettings() --> PumpSettings
00:53:57.4	pump: GetSetManualValvePosition(A) --> 240
00:53:57.4	pump: GetSetManualValvePosition(B) --> 240
00:53:57.4	-------------------------------------------------------
00:53:57.4	--- Reduce pressure ---
00:53:57.4	-------------------------------------------------------
00:53:57.4	--- Pressure channel A: 235.157043457031
00:53:57.4	--- Pressure channel B: 257.819274902344
00:53:57.4	pump: GetSettings() --> PumpSettings
00:53:57.4	------------------------------------------------------------------
00:53:57.4	PID settings changed:
00:53:57.4	------------------------------------------------------------------
00:53:57.4	P: 250 : True; Pmin: -5500 : True; Pmax: 32000 : True
00:53:57.4	I: 50 : True; Imin: -20000 : True; Imax: 20000 : True; IWindow: 50 : True
00:53:57.4	D: 1500 : True; Dmin: -10000 : True; Dmax: 10000 : True
00:53:57.4	Result min: -32000 : True; Result max: 32000 : True
00:53:57.4	------------------------------------------------------------------
00:53:57.4	pump: SetSettings(PumpSettings)
00:53:57.7	--- Set pressure channel A:          10
00:53:57.7	--- Set pressure channel B:          5
00:53:57.7	--- Reduce pressure flow controlled: True
00:54:15.7	--------------------------------------------------------
00:54:15.7	--- Log PID parameter called from: reducePressure
00:54:15.7	--------------------------------------------------------
00:54:15.7	--- P: 200; Pmin: -32000; Pmax: 32000
00:54:15.7	--- I: 200; Imin: -32000; Imax: 32000; IWindow: 100
00:54:15.7	--- D: 1000; Dmin: -32000; Dmax: 32000
00:54:15.7	--- Result min: -32000; Result max: 32000
00:54:15.7	--------------------------------------------------------
00:54:15.7	--------------------------------------------------------
00:54:15.7	--- Log instrument settings called from: reducePressure
00:54:15.7	--------------------------------------------------------
00:54:15.7	--- HomeSpeed: 2000; PurgeSpeed: 2000
00:54:15.7	--- PressureTrackingExcessFactor: 96
00:54:15.7	--- FlowResistanceA: 300; FlowResistanceB: 100; FlowResistanceOut: 150
00:54:15.7	--- FC_FlowFilterDepth: 5; FC_PressureFilterDepth: 0
00:54:15.7	--- PC_OutputFilterDepth: 0
00:54:15.7	--- CompressibilityFactorB: 2.29999995231628; PressurePIDNegativeResultDivisor: 1
00:54:15.7	--- ExternalZeroPressureOffsetA: 125.087898254395; ExternalZeroPressureOffsetB: 128.337753295898
00:54:15.7	--- ExternalPressureScalingFactorA: 0.0197199992835522; ExternalPressureScalingFactorB: 0.0197199992835522
00:54:15.7	--------------------------------------------------------
00:54:15.7	--- Final pressure channel A: 5.32045698165894
00:54:15.7	--- Final pressure channel B: 5.38356161117554
00:54:15.7	-------------------------------------------------------
00:54:15.9	autosampler-left: MoveValveDrive(Valve Drive 2, 0 deg)
00:54:16.0	pump: Manualmode_Pump_constantSpeed(A, 0)
00:54:16.0	pump: GetSetPistonSpeed(A) --> 0
00:54:16.0	pump: Manualmode_Pump_constantSpeed(B, 0)
00:54:16.0	pump: GetSetPistonSpeed(B) --> 0
00:54:16.0	pump: IsIdle() --> True
00:54:16.1	autosampler-left: MoveValveDrive(Valve Drive 1, 60 deg)
00:54:19.4	autosampler-left: MoveToHome()
00:54:19.5	pump: IsIdle() --> True
00:54:19.5	COMPLETED
