Summary

1. Notes


2. Result Statistics

Figure 1. False discovery rate (FDR) curve. X axis is the number of peptide-spectrum matches (PSM) being kept. Y axis is the corresponding FDR.



Figure 2. PSM score distribution. (a) Distribution of PEAKS peptide score; (b) Scatterplot of PEAKS peptide score versus precursor mass error.

(a)
(b)

Figure 3. De novo result validation. Distribution of residue local confidence: (a) Residues in de novo sequences validated by confident database peptide assignment; (b) Residues in "de novo only" sequences.

(a)
(b)
Table 1. Statistics of data.
# of MS Scans1013
# of MS/MS Scans1013
Table 2. Result filtration parameters.
Peptide -10lgP≥15
Protein -10lgP≥20
Proteins unique peptides≥0
De novo ALC Score≥50%
Table 3. Statistics of filtered result.
Peptide-Spectrum Matches110
Peptide Sequences60
Protein Groups12
Proteins28
Proteins (#Unique Peptides)3 (>2); 0 (=2); 22 (=1);
FDR (Peptide-Spectrum Matches)14.5%
FDR (Peptide Sequences)26.7%
FDR (Protein)67.9%
De Novo Only Spectra23
Table 4. PTM profile.
Name ∆Mass #PSM Position
Carbamidomethyl57.0272C
Deamidation.984N
Oxidation15.992M
Carbamidomethyl57.022N-term
Methyl ester14.022C-term
Sulfation79.962Y
NDA175.041N-term
Carbofuran58.031S
Dehydration-18.011N-term
DTT_ST136.001S
O-Diethylphosphate136.031Y

3. Experiment Control

Figure 4. Precursor mass error of peptide-spectrum matches (PSM) in filtered result. (a) Distribution of precursor mass error in ppm; (b) Scatterplot of precursor m/z versus precursor mass error in ppm.

(a)
(b)

Table 5. Number of identified peptides in each sample by the number of missed cleavages

Missed Cleavages01234+
Sample 283614820

4. Other Information

Table 6. Search parameters.
Query Type: Homology Match
Fixed Modifications:
  Carbamidomethylation: 57.02
Variable Modifications:
  Oxidation (M): 15.99
Fragment ion tolerance: 0.5
L equals I: true
Q equals K: true
Report number: 1
Maximum # of PTMs: 3
De novo score (ALC%) threshold: 15
Peptide hit threshold (-10logP): 30.0
Peaks run ID: 219
Merge Options: 0.1 min. 0.5 Da
Precursor Options: corrected
Charge Options: no correction
Filter Options: no filter
Process: true
Table 7. Instrument parameters.
Fractions: 28_Seg1Ev1.mzXML
Ion Source: ESI(nano-spray)
Fragmentation Mode: CID, CAD(y and b ions)
MS Scan Mode: 3D Ion Trap
MS/MS Scan Mode: 3D Ion Trap

Protein List

Protein Group Protein ID Accession -10lgP Coverage (%) #Peptides #Unique PTM Avg. Mass Description
2 10567 tr|A0A1E1WVY8|A0A1E1WVY8_TITOB 222.33 63 20 6 Y 10060 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
1 44589 sp|H1ZZI3|SCX14_TITOB 200.46 46 15 0 Y 10106 Toxin To14 OS=Tityus obscurus OX=1221240 PE=1 SV=1
3 45508 sp|C0HLM1|SCX3_TITMA 170.46 39 12 0 Y 7836 Toxin Tma3 OS=Tityus macrochirus OX=2599738 PE=1 SV=1
4 45510 sp|C0HLM0|SCX2_TITMA 102.13 29 5 0 Y 7830 Toxin Tma2 OS=Tityus macrochirus OX=2599738 PE=1 SV=1
5 46545 tr|A0A1E1WW05|A0A1E1WW05_TITOB 99.63 58 4 4 Y 8972 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
6 27165 tr|A0A1E1WVV5|A0A1E1WVV5_TITOB 71.46 38 4 4 Y 7194 Putative potassium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
7 47494 tr|A0A2L2YWB1|A0A2L2YWB1_PARTP 38.97 8 1 1 N 6816 Knottin scorpion toxin-like domain-containing protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1
7 47496 tr|A0A2S3ICR9|A0A2S3ICR9_9POAL 38.97 7 1 1 N 8110 Knottin scorpion toxin-like domain-containing protein OS=Panicum hallii OX=206008 GN=PAHAL_8G046500 PE=4 SV=1
7 47497 tr|A0A2T7CKN5|A0A2T7CKN5_9POAL 38.97 7 1 1 N 8110 Knottin scorpion toxin-like domain-containing protein OS=Panicum hallii var. hallii OX=1504633 GN=GQ55_8G044400 PE=4 SV=1
7 46657 tr|A0A453JPA3|A0A453JPA3_AEGTS 32.47 5 1 1 N 11978 Knottin scorpion toxin-like domain-containing protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 SV=1
7 46658 tr|A0A453JPC9|A0A453JPC9_AEGTS 32.47 4 1 1 N 12513 Knottin scorpion toxin-like domain-containing protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 SV=1
7 17674 tr|A0A1W7R9K5|A0A1W7R9K5_9SCOR 38.97 1 1 1 N 48916 Putative serine incorporator OS=Hadrurus spadix OX=141984 PE=3 SV=1
7 4994 tr|A0A1W7RAZ4|A0A1W7RAZ4_9SCOR 32.47 1 1 1 N 106453 Anoctamin OS=Hadrurus spadix OX=141984 PE=3 SV=1
11 6162 tr|A0A510A9R9|A0A510A9R9_MESMA 23.54 1 1 1 N 209391 LDscam3 OS=Mesobuthus martensii OX=34649 PE=2 SV=1
16 47507 tr|A0A507ZTD3|A0A507ZTD3_9ACTO 22.48 8 1 1 Y 8454 Arthropod defensin OS=Actinomyces johnsonii OX=544581 GN=FK256_14200 PE=4 SV=1
16 16596 tr|U1PXD7|U1PXD7_9ACTO 22.48 8 1 1 Y 8454 Arthropod defensin OS=Actinomyces johnsonii F0510 OX=1227262 GN=HMPREF1549_01193 PE=4 SV=1
16 47017 tr|A0A397ZYR2|A0A397ZYR2_BRACM 22.48 7 1 1 Y 9423 Knottin scorpion toxin-like domain-containing protein OS=Brassica campestris OX=3711 GN=BRARA_C02325 PE=4 SV=1
16 47020 tr|A0A508B976|A0A508B976_9ACTO 22.48 6 1 1 Y 11189 Arthropod defensin OS=Actinomyces oris OX=544580 GN=FK267_13255 PE=4 SV=1
8 34601 tr|M7YWV1|M7YWV1_TRIUA 22.07 20 1 1 Y 9168 Defensin Tk-AMP-D1 OS=Triticum urartu OX=4572 GN=TRIUR3_01070 PE=3 SV=1
12 47511 tr|A0A1V1WBR0|A0A1V1WBR0_9SCOR 21.79 9 1 1 N 6339 Putative NDBP (Fragment) OS=Superstitionia donensis OX=311983 PE=4 SV=1
12 47514 tr|A0A1V1WC73|A0A1V1WC73_9SCOR 21.79 7 1 1 N 8271 Putative NDBP OS=Superstitionia donensis OX=311983 PE=4 SV=1
12 47522 sp|P0DMF2|NDB4T_CHATY 21.79 6 1 1 N 9235 Peptide Ctry2346 OS=Chaerilus tryznai OX=1464547 PE=3 SV=1
12 47534 tr|D0UMT6|D0UMT6_HADAR 21.79 3 1 1 N 20076 SH2 domain binding protein (Fragment) OS=Hadrurus arizonensis OX=88316 PE=2 SV=1
12 21658 tr|A0A1W7RA09|A0A1W7RA09_9SCOR 21.79 1 1 1 N 45956 Poly(RC)-binding protein 3 OS=Hadrurus spadix OX=141984 PE=4 SV=1
12 10485 tr|A0A1W7RAL8|A0A1W7RAL8_9SCOR 21.79 1 1 1 N 61091 EH domain-containing protein 3 OS=Hadrurus spadix OX=141984 PE=4 SV=1
12 13586 tr|A0A1S5QMZ5|A0A1S5QMZ5_TITSE 21.79 1 1 1 N 71868 Angiotensin-converting enzyme OS=Tityus serrulatus OX=6887 PE=2 SV=1
14 47537 tr|A0A0U1SCI0|A0A0U1SCI0_ISOMC 20.56 2 1 1 N 24078 SCP domain-containing protein (Fragment) OS=Isometrus maculatus OX=497827 PE=2 SV=1
14 4987 tr|A0A077S8H7|A0A077S8H7_WHEAT 20.56 0 1 1 N 207714 Uncharacterized protein OS=Triticum aestivum OX=4565 GN=TRAES_3BF139100070CFD_c1 PE=3 SV=1
total 28 proteins

tr|A0A1E1WVY8|A0A1E1WVY8_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.NC(+57.02)MLDC(+57.02)NILDDK.G Y 78.52 1509.6262 -0.5 755.8200 2 28.31 912 3 32 43 Carbamidomethylation
K.AAC(+57.02)YC(+57.02)YGLPDDSPTK.T N 77.51 1716.7123 -15.7 859.3500 2 23.65 736 7 65 79 Carbamidomethylation
C.KKDDYPVDTAKR.N N 70.16 1434.7466 -0.8 718.3800 2 11.05 250 4 20 31
C.KKDDYPVDTAK.R N 69.53 1278.6455 0.0 640.3300 2 12.39 306 3 20 30
R.KADSGYC(+57.02)YK.L N 66.40 1090.4753 247.7 546.3800 2 7.93 124 14 54 62 Carbamidomethylation
Y.C(+57.02)YGLPDDSPTK.T N 57.19 1251.5441 1.2 626.7800 2 21.46 654 4 69 79 Carbamidomethylation
K.KDDYPVDTAK.R N 52.31 1150.5505 -4.4 576.2800 2 14.04 370 2 21 30
R.N(+.98)C(+57.02)MLDC(+57.02)NILDDK.G Y 51.27 1510.6102 605.9 756.7700 2 28.36 914 1 32 43 Deamidation (NQ); Carbamidomethylation
R.NC(+57.02)MLDC(+57.02)NILDDKGYC(+57.02)DK.F Y 48.34 2132.8635 -16.6 711.9500 3 27.77 892 1 32 48 Carbamidomethylation
R.N(+.98)C(+57.02)MLDC(+57.02)NILDDKGYC(+57.02)DKF.C Y 44.14 2280.9160 496.4 761.6900 3 32.53 1076 1 32 49 Deamidation (NQ); Carbamidomethylation
K.DDYPVDTAKR.N N 44.10 1178.5568 7.3 590.2900 2 15.69 434 2 22 31
D.C(-18.01)NILDDKGYC(+57.02)DK.F Y 42.40 1424.6064 -84.9 713.2500 2 27.99 900 1 37 48 Dehydration (C@N-term); Carbamidomethylation
K.KDDYPVDTAKR.N N 38.90 1306.6517 -4.8 654.3300 2 13.42 346 1 21 31
D.SGYC(+57.02)YK.L N 35.46 776.3163 -4.6 777.3200 1 6.38 58 4 57 62 Carbamidomethylation
R.K(+26.02)ADSGYC(+57.02)YK.L N 32.06 1116.4910 -4.9 559.2500 2 14.34 382 1 54 62 Acetaldehyde +26; Carbamidomethylation
D.YPVDTAK.R N 27.24 792.4017 -11.3 793.4000 1 13.52 350 1 24 30
R.N(+.98)C(+57.02)M(+15.99)LDC(+57.02)NILDDK.G Y 25.74 1526.6051 -654.8 763.8100 2 24.84 782 1 32 43 Deamidation (NQ); Carbamidomethylation; Oxidation (M)
L.PDDSPTK.T N 25.61 758.3446 -15.6 759.3400 1 23.16 716 2 73 79
Y.PVDTAK.R N 25.03 629.3384 1564.0 631.3300 1 12.29 300 1 25 30
I.LDDKGY(+79.96)C(+57.02)DKFC(+57.02)K.G Y 17.88 1627.6316 -605.9 814.3300 2 34.24 1138 1 40 51 Sulfation; Carbamidomethylation
D.KGY(+79.96)C(+57.02)DKFC(+57.02)K.G Y 16.51 1284.4937 24.8 643.2700 2 28.72 928 1 43 51 Sulfation; Carbamidomethylation
Y.PVDTAKR.N N 15.75 785.4395 -43.4 393.7100 2 11.61 272 1 25 31
R.KADSGYC(+57.02)YE(sub K).L N 15.66 1091.4230 625.3 547.0600 2 6.09 46 1 54 62 Carbamidomethylation; Mutation
R.NC(+57.02)MLDC(+57.02)NILDDKGYC(+57.02)DKF.C Y 15.12 2279.9319 441.4 761.3200 3 32.10 1060 1 32 49 Carbamidomethylation
I.L(+175.04)DDKGYC(+57.02)DKFC(+57.02)K.G Y 15.05 1722.7170 -88.0 862.2900 2 26.74 852 1 40 51 Naphthalene-2,3-dicarboxaldehyde; Carbamidomethylation
total 25 peptides
Best Unique PSM (Scan 912, m/z=755.8200, z=2, RT=28.31, ppm=-0.5):
sp|H1ZZI3|SCX14_TITOB
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| Protein Coverage | Supporting Peptides |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.AAC(+57.02)YC(+57.02)YGLPDDSPTK.T N 77.51 1716.7123 -15.7 859.3500 2 23.65 736 7 65 79 Carbamidomethylation
C.KKDDYPVDTAKR.N N 70.16 1434.7466 -0.8 718.3800 2 11.05 250 4 20 31
C.KKDDYPVDTAK.R N 69.53 1278.6455 0.0 640.3300 2 12.39 306 3 20 30
R.KADSGYC(+57.02)YK.L N 66.40 1090.4753 247.7 546.3800 2 7.93 124 14 54 62 Carbamidomethylation
Y.C(+57.02)YGLPDDSPTK.T N 57.19 1251.5441 1.2 626.7800 2 21.46 654 4 69 79 Carbamidomethylation
K.KDDYPVDTAK.R N 52.31 1150.5505 -4.4 576.2800 2 14.04 370 2 21 30
K.DDYPVDTAKR.N N 44.10 1178.5568 7.3 590.2900 2 15.69 434 2 22 31
R.C(+57.02)NPNV.R N 40.43 602.2482 -9.1 603.2500 1 8.80 158 9 84 88 Carbamidomethylation
K.KDDYPVDTAKR.N N 38.90 1306.6517 -4.8 654.3300 2 13.42 346 1 21 31
D.SGYC(+57.02)YK.L N 35.46 776.3163 -4.6 777.3200 1 6.38 58 4 57 62 Carbamidomethylation
R.C(+57.02)NPNV(+14.02).R N 33.90 616.2639 -1.9 617.2700 1 12.59 312 2 84 88 Carbamidomethylation; Methyl ester
R.K(+26.02)ADSGYC(+57.02)YK.L N 32.06 1116.4910 -4.9 559.2500 2 14.34 382 1 54 62 Acetaldehyde +26; Carbamidomethylation
D.YPVDTAK.R N 27.24 792.4017 -11.3 793.4000 1 13.52 350 1 24 30
L.PDDSPTK.T N 25.61 758.3446 -15.6 759.3400 1 23.16 716 2 73 79
Y.PVDTAK.R N 25.03 629.3384 1564.0 631.3300 1 12.29 300 1 25 30
Y.PVDTAKR.N N 15.75 785.4395 -43.4 393.7100 2 11.61 272 1 25 31
R.KADSGYC(+57.02)YE(sub K).L N 15.66 1091.4230 625.3 547.0600 2 6.09 46 1 54 62 Carbamidomethylation; Mutation
total 17 peptides
sp|C0HLM1|SCX3_TITMA
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| Protein Coverage | Supporting Peptides |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
KKDDYPVDTAKR.N N 70.16 1434.7466 -0.8 718.3800 2 11.05 250 4 1 12
KKDDYPVDTAK.R N 69.53 1278.6455 0.0 640.3300 2 12.39 306 3 1 11
Y.C(+57.02)YGLPDDSPTK.K N 57.19 1251.5441 1.2 626.7800 2 21.46 654 4 50 60 Carbamidomethylation
K.KDDYPVDTAK.R N 52.31 1150.5505 -4.4 576.2800 2 14.04 370 2 2 11
K.DDYPVDTAKR.N N 44.10 1178.5568 7.3 590.2900 2 15.69 434 2 3 12
K.KDDYPVDTAKR.N N 38.90 1306.6517 -4.8 654.3300 2 13.42 346 1 2 12
N.AS(+136.00)C(+57.02)YC(+57.02)YGLPDDSPTK.K N 37.48 1868.7089 -87.4 935.2800 2 24.79 780 1 46 60 Dithiothreitol (DTT); Carbamidomethylation
N.A(+57.02)S(+79.97)C(+57.02)YC(+57.02)YGLPDDSPTK.K N 35.16 1869.6951 112.5 935.9600 2 25.11 792 1 46 60 Carbamidomethylation (DHKE, X@N-term); Phosphorylation (STY); Carbamidomethylation
N.ASC(+57.02)Y(+136.03)C(+57.02)YGLPDDSPTK.K N 34.95 1868.7362 -112.8 935.2700 2 24.90 784 1 46 60 Carbamidomethylation; O-Diethylphosphorylation
A.S(+58.03)C(+57.02)YC(+57.02)YGLPDDSPTK.K N 28.41 1719.6995 -194.2 860.6900 2 23.60 734 1 47 60 2,3-dihydro-2,2-dimethyl-7-benzofuranol N-methyl carbamate; Carbamidomethylation
D.YPVDTAK.R N 27.24 792.4017 -11.3 793.4000 1 13.52 350 1 5 11
L.PDDSPTK.K N 25.61 758.3446 -15.6 759.3400 1 23.16 716 2 54 60
Y.PVDTAK.R N 25.03 629.3384 1564.0 631.3300 1 12.29 300 1 6 11
Y.PVDTAKR.N N 15.75 785.4395 -43.4 393.7100 2 11.61 272 1 6 12
total 14 peptides
sp|C0HLM0|SCX2_TITMA
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| Protein Coverage | Supporting Peptides |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Y.C(+57.02)YGLPDDSPTK.T N 57.19 1251.5441 1.2 626.7800 2 21.46 654 4 50 60 Carbamidomethylation
R.C(+57.02)NPNV N 40.43 602.2482 -9.1 603.2500 1 8.80 158 9 65 69 Carbamidomethylation
K.AS(+136.00)C(+57.02)YC(+57.02)YGLPDDSPTK.T N 37.48 1868.7089 -87.4 935.2800 2 24.79 780 1 46 60 Dithiothreitol (DTT); Carbamidomethylation
K.A(+57.02)S(+79.97)C(+57.02)YC(+57.02)YGLPDDSPTK.T N 35.16 1869.6951 112.5 935.9600 2 25.11 792 1 46 60 Carbamidomethylation (DHKE, X@N-term); Phosphorylation (STY); Carbamidomethylation
K.ASC(+57.02)Y(+136.03)C(+57.02)YGLPDDSPTK.T N 34.95 1868.7362 -112.8 935.2700 2 24.90 784 1 46 60 Carbamidomethylation; O-Diethylphosphorylation
R.C(+57.02)NPNV(+14.02) N 33.90 616.2639 -1.9 617.2700 1 12.59 312 2 65 69 Carbamidomethylation; Methyl ester
A.S(+58.03)C(+57.02)YC(+57.02)YGLPDDSPTK.T N 28.41 1719.6995 -194.2 860.6900 2 23.60 734 1 47 60 2,3-dihydro-2,2-dimethyl-7-benzofuranol N-methyl carbamate; Carbamidomethylation
L.PDDSPTK.T N 25.61 758.3446 -15.6 759.3400 1 23.16 716 2 54 60
total 8 peptides
tr|A0A1E1WW05|A0A1E1WW05_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.GC(+57.02)YC(+57.02)YDVAPSTK.M Y 52.34 1419.5798 -10.2 710.7900 2 19.49 578 2 63 74 Carbamidomethylation
R.AGATN(+.98)GKGDC(+57.02)INK.G Y 50.60 1305.5983 -9.8 653.8000 2 10.33 220 1 50 62 Deamidation (NQ); Carbamidomethylation
K.IYC(+57.02)PDDAVC(+57.02)KDTC(+57.02)K.N Y 41.92 1743.7266 -7.7 582.2450 3 18.31 534 2 34 47 Carbamidomethylation
K.MYPGRLPC(+57.02)N Y 32.06 1106.5001 4.8 554.2600 2 19.82 590 2 75 83 Carbamidomethylation
total 4 peptides
Best Unique PSM (Scan 578, m/z=710.7900, z=2, RT=19.49, ppm=-10.2):
tr|A0A1E1WVV5|A0A1E1WVV5_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.GKGTGYC(+57.02)DKDVC(+57.02)K.C Y 43.10 1486.6544 -6.0 744.3300 2 10.63 232 1 46 58 Carbamidomethylation
A.SPFGK.C Y 32.06 534.2802 -13.8 535.2800 1 9.09 170 2 28 32
K.G(+57.02)TGYCDKDVC(+57.02)K.C Y 24.78 1301.5380 5.8 651.7800 2 12.73 318 1 48 58 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
K.PLNC(+57.02)AK.E Y 16.25 701.3530 -0.5 702.3600 1 13.99 368 1 35 40 Carbamidomethylation
total 4 peptides
Best Unique PSM (Scan 232, m/z=744.3300, z=2, RT=10.63, ppm=-6.0):
tr|A0A2L2YWB1|A0A2L2YWB1_PARTP
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
H.ALFGK.C Y 38.97 534.3165 -81.9 535.2800 1 9.82 200 3 40 44
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A2S3ICR9|A0A2S3ICR9_9POAL
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
V.ALFGK.G Y 38.97 534.3165 -81.9 535.2800 1 9.82 200 3 53 57
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A2T7CKN5|A0A2T7CKN5_9POAL
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
V.ALFGK.G Y 38.97 534.3165 -81.9 535.2800 1 9.82 200 3 53 57
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A453JPA3|A0A453JPA3_AEGTS
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
M.AIFGK.S Y 32.47 534.3165 -81.9 535.2800 1 9.82 200 3 22 26
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A453JPC9|A0A453JPC9_AEGTS
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
M.AIFGK.S Y 32.47 534.3165 -81.9 535.2800 1 9.82 200 3 29 33
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A1W7R9K5|A0A1W7R9K5_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Q.ALFGK.V Y 38.97 534.3165 -81.9 535.2800 1 9.82 200 3 149 153
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A1W7RAZ4|A0A1W7RAZ4_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
N.AIFGK.R Y 32.47 534.3165 -81.9 535.2800 1 9.82 200 3 266 270
total 1 peptides
Best Unique PSM (Scan 200, m/z=535.2800, z=1, RT=9.82, ppm=-81.9):
tr|A0A510A9R9|A0A510A9R9_MESMA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.IQPSDASVLVGR.S Y 23.54 1240.6775 -138.7 621.2600 2 18.44 538 1 707 718
total 1 peptides
Best Unique PSM (Scan 538, m/z=621.2600, z=2, RT=18.44, ppm=-138.7):
tr|A0A507ZTD3|A0A507ZTD3_9ACTO
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
H.C(+57.02)LSTGY.R Y 22.48 699.2898 -10.1 700.2900 1 16.16 452 1 53 58 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 452, m/z=700.2900, z=1, RT=16.16, ppm=-10.1):
tr|U1PXD7|U1PXD7_9ACTO
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
H.C(+57.02)LSTGY.R Y 22.48 699.2898 -10.1 700.2900 1 16.16 452 1 53 58 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 452, m/z=700.2900, z=1, RT=16.16, ppm=-10.1):
tr|A0A397ZYR2|A0A397ZYR2_BRACM
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.C(+57.02)ISTGY.K Y 22.48 699.2898 -10.1 700.2900 1 16.16 452 1 46 51 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 452, m/z=700.2900, z=1, RT=16.16, ppm=-10.1):
tr|A0A508B976|A0A508B976_9ACTO
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
H.C(+57.02)LSTGY.R Y 22.48 699.2898 -10.1 700.2900 1 16.16 452 1 75 80 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 452, m/z=700.2900, z=1, RT=16.16, ppm=-10.1):
tr|M7YWV1|M7YWV1_TRIUA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.M(+15.99)AAAPAAALLIVLLLVA.T Y 22.07 1635.9996 480.4 819.4000 2 24.68 776 4 7 23 Oxidation (M)
total 1 peptides
Best Unique PSM (Scan 776, m/z=819.4000, z=2, RT=24.68, ppm=480.4):
tr|A0A1V1WBR0|A0A1V1WBR0_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.SLLGK.R Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 36 40
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
tr|A0A1V1WC73|A0A1V1WC73_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
S.SILGK.R Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 40 44
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
sp|P0DMF2|NDB4T_CHATY
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Q.SLLGK.R Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 31 35
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
tr|D0UMT6|D0UMT6_HADAR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.SLLGK.A Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 18 22
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
tr|A0A1W7RA09|A0A1W7RA09_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
G.SIIGK.K Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 31 35
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
tr|A0A1W7RAL8|A0A1W7RAL8_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
N.SILGK.I Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 481 485
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
tr|A0A1S5QMZ5|A0A1S5QMZ5_TITSE
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
L.SILGK.A Y 21.79 516.3271 -105.3 517.2800 1 9.62 192 1 110 114
total 1 peptides
Best Unique PSM (Scan 192, m/z=517.2800, z=1, RT=9.62, ppm=-105.3):
tr|A0A0U1SCI0|A0A0U1SCI0_ISOMC
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.DPTGK.L Y 20.56 516.2543 16.3 517.2700 1 9.14 172 1 82 86
total 1 peptides
Best Unique PSM (Scan 172, m/z=517.2700, z=1, RT=9.14, ppm=16.3):
tr|A0A077S8H7|A0A077S8H7_WHEAT
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
I.DPTGK.K Y 20.56 516.2543 16.3 517.2700 1 9.14 172 1 983 987
total 1 peptides
Best Unique PSM (Scan 172, m/z=517.2700, z=1, RT=9.14, ppm=16.3):
 


Prepared with PEAKS ™ (bioinfor.com)