Summary

1. Notes


2. Result Statistics

Figure 1. False discovery rate (FDR) curve. X axis is the number of peptide-spectrum matches (PSM) being kept. Y axis is the corresponding FDR.



Figure 2. PSM score distribution. (a) Distribution of PEAKS peptide score; (b) Scatterplot of PEAKS peptide score versus precursor mass error.

(a)
(b)

Figure 3. De novo result validation. Distribution of residue local confidence: (a) Residues in de novo sequences validated by confident database peptide assignment; (b) Residues in "de novo only" sequences.

(a)
(b)
Table 1. Statistics of data.
# of MS Scans987
# of MS/MS Scans987
Table 2. Result filtration parameters.
Peptide -10lgP≥15
Protein -10lgP≥20
Proteins unique peptides≥0
De novo ALC Score≥50%
Table 3. Statistics of filtered result.
Peptide-Spectrum Matches85
Peptide Sequences38
Protein Groups16
Proteins30
Proteins (#Unique Peptides)3 (>2); 14 (=2); 13 (=1);
FDR (Peptide-Spectrum Matches)8.2%
FDR (Peptide Sequences)18.4%
FDR (Protein)33.3%
De Novo Only Spectra34
Table 4. PTM profile.
Name ∆Mass #PSM Position
Carbamidomethyl57.0252C
Oxidation15.992M

3. Experiment Control

Figure 4. Precursor mass error of peptide-spectrum matches (PSM) in filtered result. (a) Distribution of precursor mass error in ppm; (b) Scatterplot of precursor m/z versus precursor mass error in ppm.

(a)
(b)

Table 5. Number of identified peptides in each sample by the number of missed cleavages

Missed Cleavages01234+
Sample 402610200

4. Other Information

Table 6. Search parameters.
Search Engine Name: PEAKS 7.0
Parent Mass Error Tolerance: 0.5 Da
Fragment Mass Error Tolerance: 0.5 Da
Precursor Mass Search Type: monoisotopic
Enzyme: Trypsin
Max Missed Cleavages: 3
Non-specific Cleavage: both
Fixed Modifications:
  Carbamidomethylation: 57.02
Variable Modifications:
  Oxidation (M): 15.99
Max variable PTM per peptide: 3
Database: Scopion2023
Taxon: All
Searched Entry: 15746
FDR Estimation: Enabled
Merge Options: no merge
Precursor Options: corrected
Charge Options: no correction
Filter Options: no filter
Process: false
Table 7. Instrument parameters.
Fractions: 40_Seg1Ev1.mzXML
Ion Source: ESI(nano-spray)
Fragmentation Mode: CID, CAD(y and b ions)
MS Scan Mode: 3D Ion Trap
MS/MS Scan Mode: 3D Ion Trap

Protein List

Protein Group Protein ID Accession -10lgP Coverage (%) #Peptides #Unique PTM Avg. Mass Description
4 62618 tr|A0A1E1WVS0|A0A1E1WVS0_TITOB 99.56 22 5 5 Y 15366 Putative vasotocin-neurophysin OS=Tityus obscurus OX=1221240 PE=3 SV=1
1 1567 sp|P60214|SCX1_TITOB 91.86 37 6 3 Y 10008 Beta-mammal/insect toxin To1 OS=Tityus obscurus OX=1221240 PE=1 SV=2
5 51036 sp|C0HLZ1|SCX10_TITPA 88.60 35 3 2 Y 7578 Toxin Tppa2 OS=Tityus pachyurus OX=288781 PE=1 SV=1
5 51037 sp|C0HLZ0|SCX9_TITPA 88.60 35 3 2 Y 7525 Toxin Tppa1 OS=Tityus pachyurus OX=288781 PE=1 SV=1
5 51038 tr|A0A7L4XUV3|A0A7L4XUV3_9SCOR 88.60 32 3 2 Y 8154 Neurotoxin Tfe2 OS=Tityus festae OX=2684172 PE=2 SV=1
5 51039 tr|A0A7L4XT36|A0A7L4XT36_TITPA 88.60 32 3 2 Y 8179 Neurotoxin TpaP2 OS=Tityus pachyurus OX=288781 PE=2 SV=1
5 51041 tr|A0A7L4XS46|A0A7L4XS46_TITPA 88.60 32 3 2 Y 8256 Neurotoxin TpaP1 OS=Tityus pachyurus OX=288781 PE=2 SV=1
5 51042 tr|A0A7L4XS27|A0A7L4XS27_9SCOR 88.60 32 3 2 Y 8256 Neurotoxin Tas1 OS=Tityus asthenes OX=2052327 PE=2 SV=1
5 51045 tr|A0A7L4XVD1|A0A7L4XVD1_TITPA 88.60 32 3 2 Y 8169 Neurotoxin TpaP5 OS=Tityus pachyurus OX=288781 PE=2 SV=1
5 51047 tr|A0A7L4XUN7|A0A7L4XUN7_9SCOR 88.60 32 3 2 Y 8256 Neurotoxin Tfe3 OS=Tityus festae OX=2684172 PE=2 SV=1
5 1565 sp|Q1I169|SCX5_TITDI 88.60 32 3 2 Y 8232 Toxin Td5 (Fragment) OS=Tityus discrepans OX=57059 PE=1 SV=1
3 45511 sp|A0A1D3IY23|TOACP_TITOB 86.38 23 2 2 N 7368 Peptide ToAcP OS=Tityus obscurus OX=1221240 PE=1 SV=1
3 27184 tr|A0A1E1WWD3|A0A1E1WWD3_TITOB 86.38 15 2 2 N 11661 Putative venom toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
2 1562 sp|P84631|SCX2_TITPA 85.63 32 4 1 Y 7531 Beta-mammal toxin Tpa2 OS=Tityus pachyurus OX=288781 PE=1 SV=1
10 22605 tr|A0A1E1WVV4|A0A1E1WVV4_TITOB 60.24 15 2 2 Y 9994 Putative potassium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
6 61939 sp|H1ZZI1|SCX12_TITOB 58.92 31 3 3 Y 9417 Toxin To12 OS=Tityus obscurus OX=1221240 PE=2 SV=1
7 291 sp|P60215|SCX4_TITOB 56.16 13 2 2 Y 10084 Beta-toxin To4 OS=Tityus obscurus OX=1221240 PE=1 SV=2
8 55679 tr|A0A1E1WW03|A0A1E1WW03_TITOB 41.63 18 2 2 Y 9273 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
19 55670 sp|H1ZZI4|SCX15_TITOB 27.29 13 1 1 Y 9230 Toxin To15 OS=Tityus obscurus OX=1221240 PE=2 SV=1
12 7701 tr|A0A2I9LPW9|A0A2I9LPW9_9SCOR 26.01 0 1 1 N 200889 Venom factor OS=Centruroides hentzi OX=88313 PE=4 SV=1
11 64469 tr|A0A6G1DLS2|A0A6G1DLS2_9ORYZ 25.54 6 1 1 Y 9840 Knottin scorpion toxin-like domain-containing protein OS=Oryza meyeriana var. granulata OX=110450 GN=E2562_019826 PE=3 SV=1
20 64464 tr|A0A1W7R9Q0|A0A1W7R9Q0_9SCOR 25.27 4 1 1 N 29712 40S ribosomal protein S4 OS=Hadrurus spadix OX=141984 PE=3 SV=1
13 44588 sp|B7SNV8|SCX8_PARGR 24.57 11 1 1 Y 9315 Toxin Pg8 OS=Parabuthus granulatus OX=242110 PE=1 SV=1
13 10567 tr|A0A1E1WVY8|A0A1E1WVY8_TITOB 24.57 10 1 1 Y 10060 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
13 44589 sp|H1ZZI3|SCX14_TITOB 24.57 10 1 1 Y 10106 Toxin To14 OS=Tityus obscurus OX=1221240 PE=1 SV=1
9 21765 tr|F1CJ20|F1CJ20_HOTJU 23.09 1 1 1 N 39999 Putative hemolectin (Fragment) OS=Hottentotta judaicus OX=6863 PE=2 SV=1
9 9307 tr|A0A2I9LP79|A0A2I9LP79_9SCOR 23.09 1 1 1 N 46132 Metalloproteinase OS=Centruroides hentzi OX=88313 PE=3 SV=1
9 23685 tr|A0A2I9LPS7|A0A2I9LPS7_9SCOR 23.09 1 1 1 N 57019 T-complex protein 1 subunit delta OS=Centruroides hentzi OX=88313 PE=3 SV=1
9 10477 tr|A0A2I9LP92|A0A2I9LP92_9SCOR 23.09 0 1 1 N 229482 Myosin heavy chain OS=Centruroides hentzi OX=88313 PE=3 SV=1
21 64465 tr|A0A0U1U046|A0A0U1U046_ISOMC 21.31 8 1 1 N 15160 40S ribosomal protein S24 OS=Isometrus maculatus OX=497827 PE=2 SV=1
total 30 proteins

tr|A0A1E1WVS0|A0A1E1WVS0_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.NSC(+57.02)GLAEENK.C Y 61.51 1120.4818 378.1 561.4600 2 11.50 254 4 36 45 Carbamidomethylation
K.RNSC(+57.02)GLAEENK.C Y 41.58 1276.5830 1.9 639.3000 2 9.93 194 2 35 45 Carbamidomethylation
R.NVNIIKR.N Y 23.79 855.5290 -7.3 856.5300 1 13.73 342 1 29 35
K.TENTFM(+15.99)K.N Y 20.92 885.3902 2.0 443.7033 2 10.86 230 2 108 114 Oxidation (M)
F.HYHLC(+57.02)R.I Y 20.46 884.4075 -13.2 443.2052 2 9.25 168 2 74 79 Carbamidomethylation
total 5 peptides
Best Unique PSM (Scan 254, m/z=561.4600, z=2, RT=11.50, ppm=378.1):
sp|P60214|SCX1_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.EGYLVGNDGC(+57.02)K.Y N 43.68 1210.5288 -2.7 606.2700 2 18.01 506 9 23 33 Carbamidomethylation
C.KKEGYLVGNDGC(+57.02)K.Y N 37.61 1466.7188 -7.2 489.9100 3 12.86 308 1 21 33 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 26 33 Carbamidomethylation
K.YGC(+57.02)ITRPHQY.C Y 33.46 1293.5924 -5.4 647.8000 2 15.97 428 3 34 43 Carbamidomethylation
Y.GC(+57.02)ITRPHQY.C Y 29.31 1130.5291 -3.2 566.2700 2 15.57 412 1 35 43 Carbamidomethylation
K.KGTDGYC(+57.02)AY.W Y 20.67 1033.4175 7.7 517.7200 2 14.94 388 1 52 60 Carbamidomethylation
total 6 peptides
Best Unique PSM (Scan 428, m/z=647.8000, z=2, RT=15.97, ppm=-5.4):
sp|C0HLZ1|SCX10_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 1 12 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 19 29 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 5 12 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
sp|C0HLZ0|SCX9_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 1 12 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 19 29 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 5 12 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
tr|A0A7L4XUV3|A0A7L4XUV3_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
tr|A0A7L4XT36|A0A7L4XT36_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
S.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
tr|A0A7L4XS46|A0A7L4XS46_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
tr|A0A7L4XS27|A0A7L4XS27_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
tr|A0A7L4XVD1|A0A7L4XVD1_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
tr|A0A7L4XUN7|A0A7L4XUN7_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
sp|Q1I169|SCX5_TITDI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y Y 53.79 1324.6082 -2.0 663.3100 2 15.20 398 2 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 46.20 1322.5132 -5.8 662.2600 2 10.76 226 3 26 36 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 12 19 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 398, m/z=663.3100, z=2, RT=15.20, ppm=-2.0):
sp|A0A1D3IY23|TOACP_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.EEDDLLGFSEEDLK.A Y 67.96 1637.7307 -3.2 819.8700 2 34.96 1138 11 35 48
R.EEDDLLGFSEEDLKA.I Y 36.84 1708.7678 583.8 855.8900 2 36.03 1176 4 35 49
total 2 peptides
Best Unique PSM (Scan 1138, m/z=819.8700, z=2, RT=34.96, ppm=-3.2):
tr|A0A1E1WWD3|A0A1E1WWD3_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.EEDDLLGFSEEDLK.A Y 67.96 1637.7307 -3.2 819.8700 2 34.96 1138 11 35 48
R.EEDDLLGFSEEDLKA.I Y 36.84 1708.7678 583.8 855.8900 2 36.03 1176 4 35 49
total 2 peptides
Best Unique PSM (Scan 1138, m/z=819.8700, z=2, RT=34.96, ppm=-3.2):
sp|P84631|SCX2_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.EGYLVGNDGC(+57.02)K.Y N 43.68 1210.5288 -2.7 606.2700 2 18.01 506 9 3 13 Carbamidomethylation
Y.C(+57.02)VHEC(+57.02)ELR.K Y 41.27 1101.4695 -3.7 551.7400 2 12.16 280 3 24 31 Carbamidomethylation
KKEGYLVGNDGC(+57.02)K.Y N 37.61 1466.7188 -7.2 489.9100 3 12.86 308 1 1 13 Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 35.09 861.4014 -0.7 431.7077 2 9.51 178 2 6 13 Carbamidomethylation
total 4 peptides
Best Unique PSM (Scan 280, m/z=551.7400, z=2, RT=12.16, ppm=-3.7):
tr|A0A1E1WVV4|A0A1E1WVV4_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.FC(+57.02)EDHC(+57.02)AAK.N Y 46.27 1136.4379 6.6 569.2300 2 10.92 232 2 67 75 Carbamidomethylation
K.LIEVK.D Y 27.95 600.3846 -3.2 601.3900 1 14.89 386 1 39 43
total 2 peptides
Best Unique PSM (Scan 232, m/z=569.2300, z=2, RT=10.92, ppm=6.6):
sp|H1ZZI1|SCX12_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.VWDYYNNK.C Y 35.41 1100.4927 -6.6 551.2500 2 20.26 592 3 73 80
F.IRPSGFC(+57.02)ER.Y Y 32.27 1120.5447 0.7 561.2800 2 14.79 382 2 36 44 Carbamidomethylation
Y.C(+57.02)YGVPANQK.V Y 22.09 1035.4807 4.6 518.7500 2 14.31 364 1 64 72 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 592, m/z=551.2500, z=2, RT=20.26, ppm=-6.6):
sp|P60215|SCX4_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.KGTFC(+57.02)AEEC(+57.02)TR.M Y 40.01 1357.5754 -7.4 679.7900 2 13.27 324 3 39 49 Carbamidomethylation
K.GTFC(+57.02)AEEC(+57.02)TR.M Y 32.29 1229.4805 817.4 616.2500 2 15.41 406 1 40 49 Carbamidomethylation
total 2 peptides
Best Unique PSM (Scan 324, m/z=679.7900, z=2, RT=13.27, ppm=-7.4):
tr|A0A1E1WW03|A0A1E1WW03_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.KADSGYC(+57.02)YWANILC(+57.02)Y.C Y 31.86 1882.8018 -72.4 942.3400 2 38.13 1250 3 49 63 Carbamidomethylation
R.KADSGYC(+57.02)YW.A Y 19.54 1148.4597 5.0 575.2400 2 22.17 664 2 49 57 Carbamidomethylation
total 2 peptides
Best Unique PSM (Scan 1250, m/z=942.3400, z=2, RT=38.13, ppm=-72.4):
sp|H1ZZI4|SCX15_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.EGYPLDSSGC(+57.02)K.A Y 27.29 1211.5128 -6.1 606.7600 2 17.40 482 1 22 32 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 482, m/z=606.7600, z=2, RT=17.40, ppm=-6.1):
tr|A0A2I9LPW9|A0A2I9LPW9_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.FPQGSK.L Y 26.01 662.3387 -54.4 663.3100 1 33.01 1068 4 313 318
total 1 peptides
Best Unique PSM (Scan 1068, m/z=663.3100, z=1, RT=33.01, ppm=-54.4):
tr|A0A6G1DLS2|A0A6G1DLS2_9ORYZ
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.EC(+57.02)ESR.C Y 25.54 679.2595 93.1 680.3300 1 23.41 710 3 34 38 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 710, m/z=680.3300, z=1, RT=23.41, ppm=93.1):
tr|A0A1W7R9Q0|A0A1W7R9Q0_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.LNAPKSWMLDK.L Y 25.27 1301.6802 4.0 651.8500 2 39.03 1282 1 12 22
total 1 peptides
Best Unique PSM (Scan 1282, m/z=651.8500, z=2, RT=39.03, ppm=4.0):
sp|B7SNV8|SCX8_PARGR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.KADSGYC(+57.02)YK.L Y 24.57 1090.4753 -9.1 546.2400 2 10.50 216 2 50 58 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 216, m/z=546.2400, z=2, RT=10.50, ppm=-9.1):
tr|A0A1E1WVY8|A0A1E1WVY8_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.KADSGYC(+57.02)YK.L Y 24.57 1090.4753 -9.1 546.2400 2 10.50 216 2 54 62 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 216, m/z=546.2400, z=2, RT=10.50, ppm=-9.1):
sp|H1ZZI3|SCX14_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.KADSGYC(+57.02)YK.L Y 24.57 1090.4753 -9.1 546.2400 2 10.50 216 2 54 62 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 216, m/z=546.2400, z=2, RT=10.50, ppm=-9.1):
tr|F1CJ20|F1CJ20_HOTJU
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.ELSIK.E Y 23.09 588.3483 -9.5 589.3500 1 8.94 156 3 197 201
total 1 peptides
Best Unique PSM (Scan 156, m/z=589.3500, z=1, RT=8.94, ppm=-9.5):
tr|A0A2I9LP79|A0A2I9LP79_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
I.ELSLK.P Y 23.09 588.3483 -9.5 589.3500 1 8.94 156 3 49 53
total 1 peptides
Best Unique PSM (Scan 156, m/z=589.3500, z=1, RT=8.94, ppm=-9.5):
tr|A0A2I9LPS7|A0A2I9LPS7_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
I.ELSLK.L Y 23.09 588.3483 -9.5 589.3500 1 8.94 156 3 424 428
total 1 peptides
Best Unique PSM (Scan 156, m/z=589.3500, z=1, RT=8.94, ppm=-9.5):
tr|A0A2I9LP92|A0A2I9LP92_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Q.ELSIK.L Y 23.09 588.3483 -9.5 589.3500 1 8.94 156 3 1270 1274
total 1 peptides
Best Unique PSM (Scan 156, m/z=589.3500, z=1, RT=8.94, ppm=-9.5):
tr|A0A0U1U046|A0A0U1U046_ISOMC
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.AKVGAASGKK Y 21.31 915.5501 -529.3 458.5400 2 14.74 380 1 124 133
total 1 peptides
Best Unique PSM (Scan 380, m/z=458.5400, z=2, RT=14.74, ppm=-529.3):
 


Prepared with PEAKS ™ (bioinfor.com)