Summary

1. Notes


2. Result Statistics

Figure 1. False discovery rate (FDR) curve. X axis is the number of peptide-spectrum matches (PSM) being kept. Y axis is the corresponding FDR.



Figure 2. PSM score distribution. (a) Distribution of PEAKS peptide score; (b) Scatterplot of PEAKS peptide score versus precursor mass error.

(a)
(b)

Figure 3. De novo result validation. Distribution of residue local confidence: (a) Residues in de novo sequences validated by confident database peptide assignment; (b) Residues in "de novo only" sequences.

(a)
(b)
Table 1. Statistics of data.
# of MS Scans1015
# of MS/MS Scans1015
Table 2. Result filtration parameters.
Peptide -10lgP≥15
Protein -10lgP≥20
Proteins unique peptides≥0
De novo ALC Score≥50%
Table 3. Statistics of filtered result.
Peptide-Spectrum Matches196
Peptide Sequences72
Protein Groups21
Proteins41
Proteins (#Unique Peptides)7 (>2); 3 (=2); 30 (=1);
FDR (Peptide-Spectrum Matches)9.2%
FDR (Peptide Sequences)23.6%
FDR (Protein)39.0%
De Novo Only Spectra28
Table 4. PTM profile.
Name ∆Mass #PSM Position
Carbamidomethyl57.0252C
Oxidation15.991M

3. Experiment Control

Figure 4. Precursor mass error of peptide-spectrum matches (PSM) in filtered result. (a) Distribution of precursor mass error in ppm; (b) Scatterplot of precursor m/z versus precursor mass error in ppm.

(a)
(b)

Table 5. Number of identified peptides in each sample by the number of missed cleavages

Missed Cleavages01234+
Sample 345217210

4. Other Information

Table 6. Search parameters.
Search Engine Name: PEAKS 7.0
Parent Mass Error Tolerance: 0.5 Da
Fragment Mass Error Tolerance: 0.5 Da
Precursor Mass Search Type: monoisotopic
Enzyme: Trypsin
Max Missed Cleavages: 3
Non-specific Cleavage: both
Fixed Modifications:
  Carbamidomethylation: 57.02
Variable Modifications:
  Oxidation (M): 15.99
Max variable PTM per peptide: 3
Database: Scopion2023
Taxon: All
Searched Entry: 15746
FDR Estimation: Enabled
Merge Options: 0.1 min. 0.5 Da
Precursor Options: corrected
Charge Options: no correction
Filter Options: no filter
Process: true
Table 7. Instrument parameters.
Fractions: 34_Seg1Ev1.mzXML
Ion Source: ESI(nano-spray)
Fragmentation Mode: CID, CAD(y and b ions)
MS Scan Mode: 3D Ion Trap
MS/MS Scan Mode: 3D Ion Trap

Protein List

Protein Group Protein ID Accession -10lgP Coverage (%) #Peptides #Unique PTM Avg. Mass Description
2 46991 tr|A0A1E1WWK1|A0A1E1WWK1_TITOB 151.33 57 10 7 Y 9302 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
1 45511 sp|A0A1D3IY23|TOACP_TITOB 119.70 22 4 4 N 7368 Peptide ToAcP OS=Tityus obscurus OX=1221240 PE=1 SV=1
1 27184 tr|A0A1E1WWD3|A0A1E1WWD3_TITOB 119.70 14 4 4 N 11661 Putative venom toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
3 46993 tr|A0A1E1WW26|A0A1E1WW26_TITOB 118.95 34 6 3 Y 9295 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
5 22605 tr|A0A1E1WVV4|A0A1E1WVV4_TITOB 90.36 52 7 7 Y 9994 Putative potassium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
4 46545 tr|A0A1E1WW05|A0A1E1WW05_TITOB 83.96 28 5 2 Y 8972 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
9 10567 tr|A0A1E1WVY8|A0A1E1WVY8_TITOB 77.91 40 3 3 Y 10060 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
9 44589 sp|H1ZZI3|SCX14_TITOB 77.91 40 3 3 Y 10106 Toxin To14 OS=Tityus obscurus OX=1221240 PE=1 SV=1
15 6157 sp|P00760|TRY1_BOVIN 67.48 11 2 2 N 25785 Serine protease 1 OS=Bos taurus OX=9913 GN=PRSS1 PE=1 SV=3
16 44510 tr|A0A1E1WVN4|A0A1E1WVN4_TITOB 55.88 18 2 2 Y 9938 Putative insulin-like protein growth factor binding protein OS=Tityus obscurus OX=1221240 PE=4 SV=1
7 4934 sp|F1LMY4|RYR1_RAT 42.41 1 4 1 Y 565491 Ryanodine receptor 1 OS=Rattus norvegicus OX=10116 GN=Ryr1 PE=1 SV=1
17 17373 tr|A0A1E1WVU5|A0A1E1WVU5_TITOB 40.04 16 1 1 Y 6863 Putative potassium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
6 4929 sp|P21817|RYR1_HUMAN 36.14 1 4 1 Y 565184 Ryanodine receptor 1 OS=Homo sapiens OX=9606 GN=RYR1 PE=1 SV=3
6 4936 sp|P16960|RYR1_PIG 36.14 1 4 1 Y 565340 Ryanodine receptor 1 OS=Sus scrofa OX=9823 GN=RYR1 PE=2 SV=2
33 1563 sp|C0HLL9|SCX1_TITMA 32.45 20 1 1 Y 7396 Beta-mammal toxin Tma1 OS=Tityus macrochirus OX=2599738 PE=1 SV=1
33 1567 sp|P60214|SCX1_TITOB 32.45 15 1 1 Y 10008 Beta-mammal/insect toxin To1 OS=Tityus obscurus OX=1221240 PE=1 SV=2
13 5085 tr|A0A8T2AQ62|A0A8T2AQ62_ARASU 29.09 1 2 1 Y 158839 Endonuclease/exonuclease/phosphatase superfamily OS=Arabidopsis suecica OX=45249 GN=ISN44_As09g029250 PE=4 SV=1
10 47119 tr|A0A1W7RAE7|A0A1W7RAE7_9SCOR 28.59 1 2 0 Y 89409 Integrin beta OS=Hadrurus spadix OX=141984 PE=3 SV=1
14 52959 tr|V5HEG3|V5HEG3_IXORI 24.39 7 1 1 N 7938 Putative preprodefensin 2 (Fragment) OS=Ixodes ricinus OX=34613 PE=2 SV=1
14 52960 tr|Q2HYY9|Q2HYY9_IXORI 24.39 7 1 1 N 8229 Preprodefensin 2 (Fragment) OS=Ixodes ricinus OX=34613 PE=2 SV=1
14 52961 tr|E3SS15|E3SS15_IXORI 24.39 7 1 1 N 8232 Defensin OS=Ixodes ricinus OX=34613 PE=4 SV=1
14 52962 tr|E3SRW7|E3SRW7_9ACAR 24.39 7 1 1 N 8233 Defensin OS=Ixodes inopinatus OX=1538109 PE=4 SV=1
14 52963 tr|Q7YXK5|Q7YXK5_IXORI 24.39 7 1 1 N 8492 Preprodefensin (Fragment) OS=Ixodes ricinus OX=34613 PE=2 SV=1
14 52964 tr|V5IDE1|V5IDE1_IXORI 24.39 7 1 1 N 8569 Putative preprodefensin 2 OS=Ixodes ricinus OX=34613 PE=2 SV=1
14 52965 tr|B7P9L7|B7P9L7_IXOSC 24.39 7 1 1 N 8477 Preprodefensin, putative OS=Ixodes scapularis OX=6945 GN=8026613 PE=4 SV=1
14 52966 tr|B7QG72|B7QG72_IXOSC 24.39 7 1 1 N 8396 Preprodefensin, putative OS=Ixodes scapularis OX=6945 GN=8040788 PE=4 SV=1
14 52967 tr|V5IBV7|V5IBV7_IXORI 24.39 7 1 1 N 8521 Putative preprodefensin OS=Ixodes ricinus OX=34613 PE=2 SV=1
14 4976 tr|A0A1W7R9N2|A0A1W7R9N2_9SCOR 24.39 0 1 1 N 577007 Ryanodine receptor OS=Hadrurus spadix OX=141984 PE=4 SV=1
34 48497 tr|B7PPN2|B7PPN2_IXOSC 24.22 7 1 1 N 8424 Preprodefensin, putative OS=Ixodes scapularis OX=6945 GN=IscW_ISCW024381 PE=4 SV=1
34 48499 tr|A0A396GBT5|A0A396GBT5_MEDTR 24.22 5 1 1 N 10885 Putative defensin, plant OS=Medicago truncatula OX=3880 GN=MtrunA17_Chr8g0339691 PE=4 SV=1
34 20750 tr|A0A1W7R9P0|A0A1W7R9P0_9SCOR 24.22 2 1 1 N 31126 Ryncolin OS=Hadrurus spadix OX=141984 PE=4 SV=1
34 20751 tr|A0A1W7R9M0|A0A1W7R9M0_9SCOR 24.22 2 1 1 N 31718 Ryncolin OS=Hadrurus spadix OX=141984 PE=4 SV=1
34 7541 tr|A0A2I9LP25|A0A2I9LP25_9SCOR 24.22 1 1 1 N 72654 Heat shock 70 kDa protein OS=Centruroides hentzi OX=88313 PE=3 SV=1
34 13783 tr|A0A510A7E9|A0A510A7E9_MESMA 24.22 0 1 1 N 159385 Dscam5 OS=Mesobuthus martensii OX=34649 PE=2 SV=1
31 18 sp|Q15858|SCN9A_HUMAN 24.06 0 1 1 N 226370 Sodium channel protein type 9 subunit alpha OS=Homo sapiens OX=9606 GN=SCN9A PE=1 SV=3
55 18581 tr|A0A1E1WVT9|A0A1E1WVT9_TITOB 21.31 1 1 1 N 41886 CLIP domain-containing serine protease (Fragment) OS=Tityus obscurus OX=1221240 PE=3 SV=1
55 7523 tr|A0A2I9LNX9|A0A2I9LNX9_9SCOR 21.31 1 1 1 N 84169 N-acetylated-alpha-linked acidic dipeptidase OS=Centruroides hentzi OX=88313 PE=3 SV=1
55 18488 tr|A0A1W7R9I0|A0A1W7R9I0_9SCOR 21.31 1 1 1 N 101225 Staphylococcal nuclease domain-containing protein OS=Hadrurus spadix OX=141984 PE=4 SV=1
37 17535 tr|A0A159W6N5|A0A159W6N5_MESMA 21.05 1 1 1 Y 79706 Dscam (Fragment) OS=Mesobuthus martensii OX=34649 GN=Dscam PE=2 SV=1
25 45750 tr|A0A1W7RA43|A0A1W7RA43_9SCOR 20.67 1 1 1 N 85879 Neprilysin OS=Hadrurus spadix OX=141984 PE=3 SV=1
22 5178 tr|A0A510A1Z1|A0A510A1Z1_MESMA 20.43 0 1 1 N 172171 Dscam14 OS=Mesobuthus martensii OX=34649 PE=2 SV=1
total 41 proteins

tr|A0A1E1WWK1|A0A1E1WWK1_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.NC(+57.02)YC(+57.02)YDVAPGTK.M Y 78.31 1446.5908 -3.7 724.3000 2 19.65 582 10 63 74 Carbamidomethylation
K.IYC(+57.02)PNDEVC(+57.02)KDTC(+57.02)K.N Y 56.73 1800.7480 189.0 601.3701 3 17.62 502 4 34 47 Carbamidomethylation
K.MYPGRLPC(+57.02)N.A N 46.46 1106.5001 -13.3 554.2500 2 19.17 562 6 75 83 Carbamidomethylation
R.AGATNGRGDC(+57.02)IWK.N Y 37.75 1404.6569 -8.1 703.3300 2 16.42 452 2 50 62 Carbamidomethylation
K.IYC(+57.02)PNDEVC(+57.02)K.D Y 32.02 1296.5479 -1.8 649.2800 2 17.28 486 1 34 43 Carbamidomethylation
C.PNDEVC(+57.02)KDTC(+57.02)K.N Y 27.79 1364.5701 -3.4 683.2900 2 17.33 488 1 37 47 Carbamidomethylation
K.M(+15.99)YPGR.L N 20.39 638.2846 -3.0 639.2900 1 8.42 140 2 75 79 Oxidation (M)
Y.PGRLPC(+57.02)N.A N 18.15 812.3963 -30.2 407.1932 2 19.07 558 3 77 83 Carbamidomethylation
Y.C(+57.02)PNDEVC(+57.02)KDTC(+57.02)K.N Y 17.78 1524.6007 -10.0 763.3000 2 17.42 492 1 36 47 Carbamidomethylation
N.GRGDC(+57.02)IWK.N Y 15.30 990.4705 -5.1 496.2400 2 18.31 528 2 55 62 Carbamidomethylation
total 10 peptides
Best Unique PSM (Scan 582, m/z=724.3000, z=2, RT=19.65, ppm=-3.7):
sp|A0A1D3IY23|TOACP_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.EEDDLLGFSEEDLK.A Y 68.00 1637.7307 314.3 820.1300 2 53.31 1856 101 35 48
E.DDLLGFSEEDLK.A Y 58.51 1379.6456 -0.1 690.8300 2 34.04 1128 1 37 48
D.DLLGFSEEDLK.A Y 44.71 1264.6187 -10.4 633.3100 2 34.15 1132 2 38 48
F.SEEDLK.A Y 30.15 719.3337 -1.4 720.3400 1 35.10 1170 1 43 48
total 4 peptides
Best Unique PSM (Scan 1856, m/z=820.1300, z=2, RT=53.31, ppm=314.3):
tr|A0A1E1WWD3|A0A1E1WWD3_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.EEDDLLGFSEEDLK.A Y 68.00 1637.7307 314.3 820.1300 2 53.31 1856 101 35 48
E.DDLLGFSEEDLK.A Y 58.51 1379.6456 -0.1 690.8300 2 34.04 1128 1 37 48
D.DLLGFSEEDLK.A Y 44.71 1264.6187 -10.4 633.3100 2 34.15 1132 2 38 48
F.SEEDLK.A Y 30.15 719.3337 -1.4 720.3400 1 35.10 1170 1 43 48
total 4 peptides
Best Unique PSM (Scan 1856, m/z=820.1300, z=2, RT=53.31, ppm=314.3):
tr|A0A1E1WW26|A0A1E1WW26_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.FHC(+57.02)SDDAMC(+57.02)K.E Y 76.70 1269.4576 6.1 635.7400 2 13.47 340 2 34 43 Carbamidomethylation
E.MYPGRLPC(+57.02)N.P N 46.46 1106.5001 -13.3 554.2500 2 19.17 562 6 75 83 Carbamidomethylation
A.LDGYPLAK.N Y 23.28 875.4752 -23.3 438.7347 2 20.01 596 2 21 28
E.M(+15.99)YPGR.L N 20.39 638.2846 -3.0 639.2900 1 8.42 140 2 75 79 Oxidation (M)
Y.PGRLPC(+57.02)N.P N 18.15 812.3963 -30.2 407.1932 2 19.07 558 3 77 83 Carbamidomethylation
E.MYPGRLPC(+57.02)NPY Y 15.13 1366.6162 -7.9 684.3100 2 24.67 774 2 75 85 Carbamidomethylation
total 6 peptides
Best Unique PSM (Scan 340, m/z=635.7400, z=2, RT=13.47, ppm=6.1):
tr|A0A1E1WVV4|A0A1E1WVV4_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.NAIGKC(+57.02)DDFK.C Y 40.94 1166.5389 -885.9 583.7600 2 14.38 374 3 76 85 Carbamidomethylation
K.LIEVKDKAK.A Y 38.08 1042.6385 476.6 522.5750 2 12.46 302 1 39 47
C.PVIDKFC(+57.02)EDHC(+57.02)AAK.N Y 34.94 1688.7650 -23.4 845.3700 2 25.18 794 1 62 75 Carbamidomethylation
K.C(+57.02)DDFK.C Y 28.92 683.2585 -8.4 684.2600 1 6.29 54 1 81 85 Carbamidomethylation
E.YAC(+57.02)PVIDKFC(+57.02)EDHC(+57.02)AAK.N Y 24.24 2082.8960 481.2 695.6400 3 25.67 814 1 59 75 Carbamidomethylation
K.C(+57.02)QC(+57.02)LKS Y 22.20 794.3415 1.5 795.3500 1 5.10 6 1 86 91 Carbamidomethylation
K.AGWEK.L Y 20.62 589.2860 -5.6 590.2900 1 8.02 124 1 48 52
total 7 peptides
Best Unique PSM (Scan 374, m/z=583.7600, z=2, RT=14.38, ppm=-885.9):
tr|A0A1E1WW05|A0A1E1WW05_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.MYPGRLPC(+57.02)N N 46.46 1106.5001 -13.3 554.2500 2 19.17 562 6 75 83 Carbamidomethylation
K.IYC(+57.02)PDDAVC(+57.02)K.D Y 35.09 1239.5264 -16.9 620.7600 2 18.67 542 1 34 43 Carbamidomethylation
K.IYC(+57.02)PDDAVC(+57.02)KDTC(+57.02)K.N Y 33.67 1743.7266 0.9 582.2500 3 18.26 526 1 34 47 Carbamidomethylation
K.M(+15.99)YPGR.L N 20.39 638.2846 -3.0 639.2900 1 8.42 140 2 75 79 Oxidation (M)
Y.PGRLPC(+57.02)N N 18.15 812.3963 -30.2 407.1932 2 19.07 558 3 77 83 Carbamidomethylation
total 5 peptides
Best Unique PSM (Scan 542, m/z=620.7600, z=2, RT=18.67, ppm=-16.9):
tr|A0A1E1WVY8|A0A1E1WVY8_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.AAC(+57.02)YC(+57.02)YGLPDDSPTK.T Y 44.66 1716.7123 -4.0 859.3600 2 23.94 746 1 65 79 Carbamidomethylation
C.KKDDYPVDTAKR.N Y 40.07 1434.7466 -0.8 718.3800 2 12.18 290 1 20 31
R.KADSGYC(+57.02)YK.L Y 39.62 1090.4753 -9.1 546.2400 2 10.60 226 1 54 62 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 746, m/z=859.3600, z=2, RT=23.94, ppm=-4.0):
sp|H1ZZI3|SCX14_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.AAC(+57.02)YC(+57.02)YGLPDDSPTK.T Y 44.66 1716.7123 -4.0 859.3600 2 23.94 746 1 65 79 Carbamidomethylation
C.KKDDYPVDTAKR.N Y 40.07 1434.7466 -0.8 718.3800 2 12.18 290 1 20 31
R.KADSGYC(+57.02)YK.L Y 39.62 1090.4753 -9.1 546.2400 2 10.60 226 1 54 62 Carbamidomethylation
total 3 peptides
Best Unique PSM (Scan 746, m/z=859.3600, z=2, RT=23.94, ppm=-4.0):
sp|P00760|TRY1_BOVIN
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.LGEDNINVVEGNEQFISASK.S Y 53.44 2162.0491 -94.1 1081.9301 2 30.80 1008 1 73 92
K.SGIQVR.L Y 28.07 658.3762 -5.3 659.3800 1 12.02 284 1 67 72
total 2 peptides
Best Unique PSM (Scan 1008, m/z=1081.9301, z=2, RT=30.80, ppm=-94.1):
tr|A0A1E1WVN4|A0A1E1WVN4_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.KDC(+57.02)PAGIVK.D Y 47.98 986.5219 -16.6 494.2600 2 12.75 312 1 35 43 Carbamidomethylation
R.NISGIC(+57.02)GK.G Y 15.79 847.4222 0.6 848.4300 1 14.22 368 1 65 72 Carbamidomethylation
total 2 peptides
Best Unique PSM (Scan 312, m/z=494.2600, z=2, RT=12.75, ppm=-16.6):
sp|F1LMY4|RYR1_RAT
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
L.EEGGENVEEGGEEE.E Y 23.60 1491.5485 735.5 747.3300 2 26.49 846 3 3735 3748
M.C(+57.02)PDIPVL.E N 17.95 812.4102 -47.3 407.1932 2 19.60 580 1 3237 3243 Carbamidomethylation
W.NPC(+57.02)GG.E N 17.58 503.1798 2072.6 505.2300 1 10.26 212 1 2323 2327 Carbamidomethylation
R.C(+57.02)APEMHLIQAGK.G N 15.86 1353.6533 -655.9 677.3900 2 53.98 1880 1 2435 2446 Carbamidomethylation
total 4 peptides
Best Unique PSM (Scan 846, m/z=747.3300, z=2, RT=26.49, ppm=735.5):
tr|A0A1E1WVU5|A0A1E1WVU5_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.SDC(+57.02)KPDLC(+57.02)EK.A Y 40.04 1250.5271 -1.3 626.2700 2 13.37 336 2 32 41 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 336, m/z=626.2700, z=2, RT=13.37, ppm=-1.3):
sp|P21817|RYR1_HUMAN
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
M.C(+57.02)PDIPVL.E N 17.95 812.4102 -47.3 407.1932 2 19.60 580 1 3240 3246 Carbamidomethylation
W.NPC(+57.02)GG.E N 17.58 503.1798 2072.6 505.2300 1 10.26 212 1 2324 2328 Carbamidomethylation
S.MTAAETAR.R Y 16.29 849.4014 -116.2 850.3100 1 9.70 190 1 1986 1993
R.C(+57.02)APEMHLIQAGK.G N 15.86 1353.6533 -655.9 677.3900 2 53.98 1880 1 2436 2447 Carbamidomethylation
total 4 peptides
Best Unique PSM (Scan 190, m/z=850.3100, z=1, RT=9.70, ppm=-116.2):
sp|P16960|RYR1_PIG
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
M.C(+57.02)PDIPVL.E N 17.95 812.4102 -47.3 407.1932 2 19.60 580 1 3241 3247 Carbamidomethylation
W.NPC(+57.02)GG.E N 17.58 503.1798 2072.6 505.2300 1 10.26 212 1 2325 2329 Carbamidomethylation
T.MTAAETAR.R Y 16.29 849.4014 -116.2 850.3100 1 9.70 190 1 1987 1994
R.C(+57.02)APEMHLIQAGK.G N 15.86 1353.6533 -655.9 677.3900 2 53.98 1880 1 2437 2448 Carbamidomethylation
total 4 peptides
Best Unique PSM (Scan 190, m/z=850.3100, z=1, RT=9.70, ppm=-116.2):
sp|C0HLL9|SCX1_TITMA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
KKEGYLVGNDGC(+57.02)K.Y Y 32.45 1466.7188 4.6 734.3700 2 13.22 330 1 1 13 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 330, m/z=734.3700, z=2, RT=13.22, ppm=4.6):
sp|P60214|SCX1_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KKEGYLVGNDGC(+57.02)K.Y Y 32.45 1466.7188 4.6 734.3700 2 13.22 330 1 21 33 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 330, m/z=734.3700, z=2, RT=13.22, ppm=4.6):
tr|A0A8T2AQ62|A0A8T2AQ62_ARASU
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
A.IISAL.P N 20.97 515.3319 1.6 516.3400 1 23.03 712 1 955 959
V.HC(+57.02)PPK.I Y 16.23 637.3006 1541.0 639.2900 1 9.08 166 1 1016 1020 Carbamidomethylation
total 2 peptides
Best Unique PSM (Scan 166, m/z=639.2900, z=1, RT=9.08, ppm=1541.0):
tr|A0A1W7RAE7|A0A1W7RAE7_9SCOR
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| Protein Coverage | Supporting Peptides |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
G.LLGGI.V N 19.80 471.3057 -6.2 472.3100 1 23.84 742 2 290 294
E.NPC(+57.02)GG.D N 17.58 503.1798 2072.6 505.2300 1 10.26 212 1 205 209 Carbamidomethylation
total 2 peptides
tr|V5HEG3|V5HEG3_IXORI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
D.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 20 24
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|Q2HYY9|Q2HYY9_IXORI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
D.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|E3SS15|E3SS15_IXORI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|E3SRW7|E3SRW7_9ACAR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
E.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|Q7YXK5|Q7YXK5_IXORI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
E.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|V5IDE1|V5IDE1_IXORI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
D.EGGEK.Q Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|B7P9L7|B7P9L7_IXOSC
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
E.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|B7QG72|B7QG72_IXOSC
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
G.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|V5IBV7|V5IBV7_IXORI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
E.EGGEK.E Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 26 30
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|A0A1W7R9N2|A0A1W7R9N2_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
T.EGGEK.G Y 24.39 518.2336 36.8 519.2600 1 7.43 100 2 4325 4329
total 1 peptides
Best Unique PSM (Scan 100, m/z=519.2600, z=1, RT=7.43, ppm=36.8):
tr|B7PPN2|B7PPN2_IXOSC
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
N.KEGEK.D Y 24.22 589.3071 -41.4 590.2900 1 8.62 148 1 26 30
total 1 peptides
Best Unique PSM (Scan 148, m/z=590.2900, z=1, RT=8.62, ppm=-41.4):
tr|A0A396GBT5|A0A396GBT5_MEDTR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
E.KEGEK.L Y 24.22 589.3071 -41.4 590.2900 1 8.62 148 1 79 83
total 1 peptides
Best Unique PSM (Scan 148, m/z=590.2900, z=1, RT=8.62, ppm=-41.4):
tr|A0A1W7R9P0|A0A1W7R9P0_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
D.KEGEK.R Y 24.22 589.3071 -41.4 590.2900 1 8.62 148 1 159 163
total 1 peptides
Best Unique PSM (Scan 148, m/z=590.2900, z=1, RT=8.62, ppm=-41.4):
tr|A0A1W7R9M0|A0A1W7R9M0_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
D.KEGEK.R Y 24.22 589.3071 -41.4 590.2900 1 8.62 148 1 159 163
total 1 peptides
Best Unique PSM (Scan 148, m/z=590.2900, z=1, RT=8.62, ppm=-41.4):
tr|A0A2I9LP25|A0A2I9LP25_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.KEGEK.N Y 24.22 589.3071 -41.4 590.2900 1 8.62 148 1 217 221
total 1 peptides
Best Unique PSM (Scan 148, m/z=590.2900, z=1, RT=8.62, ppm=-41.4):
tr|A0A510A7E9|A0A510A7E9_MESMA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
A.KEGEK.V Y 24.22 589.3071 -41.4 590.2900 1 8.62 148 1 249 253
total 1 peptides
Best Unique PSM (Scan 148, m/z=590.2900, z=1, RT=8.62, ppm=-41.4):
sp|Q15858|SCN9A_HUMAN
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.GDAEK.L Y 24.06 518.2336 17.5 519.2500 1 6.95 80 1 495 499
total 1 peptides
Best Unique PSM (Scan 80, m/z=519.2500, z=1, RT=6.95, ppm=17.5):
tr|A0A1E1WVT9|A0A1E1WVT9_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.GISDK.T Y 21.31 518.2700 5.3 519.2800 1 39.22 1326 1 118 122
total 1 peptides
Best Unique PSM (Scan 1326, m/z=519.2800, z=1, RT=39.22, ppm=5.3):
tr|A0A2I9LNX9|A0A2I9LNX9_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
S.GISDK.L Y 21.31 518.2700 5.3 519.2800 1 39.22 1326 1 610 614
total 1 peptides
Best Unique PSM (Scan 1326, m/z=519.2800, z=1, RT=39.22, ppm=5.3):
tr|A0A1W7R9I0|A0A1W7R9I0_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
N.GLSDK.I Y 21.31 518.2700 5.3 519.2800 1 39.22 1326 1 426 430
total 1 peptides
Best Unique PSM (Scan 1326, m/z=519.2800, z=1, RT=39.22, ppm=5.3):
tr|A0A159W6N5|A0A159W6N5_MESMA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
A.IC(+57.02)SVK.E Y 21.05 605.3207 -46.2 606.3000 1 16.98 474 1 22 26 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 474, m/z=606.3000, z=1, RT=16.98, ppm=-46.2):
tr|A0A1W7RA43|A0A1W7RA43_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
I.IEQFQNK.T Y 20.67 905.4606 27.4 453.7500 2 6.34 56 1 162 168
total 1 peptides
Best Unique PSM (Scan 56, m/z=453.7500, z=2, RT=6.34, ppm=27.4):
tr|A0A510A1Z1|A0A510A1Z1_MESMA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
S.VSAAPK.W Y 20.43 571.3329 1767.4 573.3500 1 50.06 1732 1 698 703
total 1 peptides
Best Unique PSM (Scan 1732, m/z=573.3500, z=1, RT=50.06, ppm=1767.4):
 


Prepared with PEAKS ™ (bioinfor.com)