Summary

1. Notes


2. Result Statistics

Figure 1. False discovery rate (FDR) curve. X axis is the number of peptide-spectrum matches (PSM) being kept. Y axis is the corresponding FDR.



Figure 2. PSM score distribution. (a) Distribution of PEAKS peptide score; (b) Scatterplot of PEAKS peptide score versus precursor mass error.

(a)
(b)

Figure 3. De novo result validation. Distribution of residue local confidence: (a) Residues in de novo sequences validated by confident database peptide assignment; (b) Residues in "de novo only" sequences.

(a)
(b)
Table 1. Statistics of data.
# of MS Scans989
# of MS/MS Scans989
Table 2. Result filtration parameters.
Peptide -10lgP≥15
Protein -10lgP≥20
Proteins unique peptides≥0
De novo ALC Score≥50%
Table 3. Statistics of filtered result.
Peptide-Spectrum Matches108
Peptide Sequences77
Protein Groups18
Proteins34
Proteins (#Unique Peptides)4 (>2); 6 (=2); 24 (=1);
FDR (Peptide-Spectrum Matches)20.4%
FDR (Peptide Sequences)28.6%
FDR (Protein)38.2%
De Novo Only Spectra21
Table 4. PTM profile.
Name ∆Mass #PSM Position
Carbamidomethyl57.0263C
Carbamidomethyl57.024D,N-term
Methyl ester14.024D,C-term
Oxidation15.992M
Phosphorylation79.971ST
Dimethylation28.031R
Diphthamide143.121H
Dehydration-18.011Y

3. Experiment Control

Figure 4. Precursor mass error of peptide-spectrum matches (PSM) in filtered result. (a) Distribution of precursor mass error in ppm; (b) Scatterplot of precursor m/z versus precursor mass error in ppm.

(a)
(b)

Table 5. Number of identified peptides in each sample by the number of missed cleavages

Missed Cleavages01234+
Sample 385915300

4. Other Information

Table 6. Search parameters.
Query Type: Homology Match
Fixed Modifications:
  Carbamidomethylation: 57.02
Variable Modifications:
  Oxidation (M): 15.99
Fragment ion tolerance: 0.5
L equals I: true
Q equals K: true
Report number: 1
Maximum # of PTMs: 3
De novo score (ALC%) threshold: 15
Peptide hit threshold (-10logP): 30.0
Peaks run ID: 281
Merge Options: 0.1 min. 0.5 Da
Precursor Options: corrected
Charge Options: no correction
Filter Options: no filter
Process: true
Table 7. Instrument parameters.
Fractions: 38_Seg1Ev1.mzXML
Ion Source: ESI(nano-spray)
Fragmentation Mode: CID, CAD(y and b ions)
MS Scan Mode: 3D Ion Trap
MS/MS Scan Mode: 3D Ion Trap

Protein List

Protein Group Protein ID Accession -10lgP Coverage (%) #Peptides #Unique PTM Avg. Mass Description
2 55670 sp|H1ZZI4|SCX15_TITOB 155.87 64 8 8 Y 9230 Toxin To15 OS=Tityus obscurus OX=1221240 PE=2 SV=1
4 1562 sp|P84631|SCX2_TITPA 144.01 32 7 2 Y 7531 Beta-mammal toxin Tpa2 OS=Tityus pachyurus OX=288781 PE=1 SV=1
5 1567 sp|P60214|SCX1_TITOB 139.74 27 6 1 Y 10008 Beta-mammal/insect toxin To1 OS=Tityus obscurus OX=1221240 PE=1 SV=2
1 61939 sp|H1ZZI1|SCX12_TITOB 128.28 51 9 9 Y 9417 Toxin To12 OS=Tityus obscurus OX=1221240 PE=2 SV=1
3 51039 tr|A0A7L4XT36|A0A7L4XT36_TITPA 120.64 42 7 3 Y 8179 Neurotoxin TpaP2 OS=Tityus pachyurus OX=288781 PE=2 SV=1
7 51040 tr|A0A7L4XVE5|A0A7L4XVE5_9SCOR 110.32 40 6 2 Y 8247 Neurotoxin Ttr2 OS=Tityus trinitatis OX=288786 PE=2 SV=1
7 51043 tr|A0A7L4XVB3|A0A7L4XVB3_9SCOR 110.32 40 6 2 Y 8346 Neurotoxin Tde1 OS=Tityus dedoslargos OX=2684171 PE=2 SV=1
7 1566 sp|P60213|SCX3_TITOB 110.32 34 6 2 Y 9903 Toxin To3 OS=Tityus obscurus OX=1221240 PE=1 SV=3
7 292 sp|H1ZZI0|SCX11_TITOB 110.32 34 6 2 Y 9984 Toxin To11 OS=Tityus obscurus OX=1221240 PE=2 SV=1
9 62618 tr|A0A1E1WVS0|A0A1E1WVS0_TITOB 85.31 15 4 4 Y 15366 Putative vasotocin-neurophysin OS=Tityus obscurus OX=1221240 PE=3 SV=1
6 22605 tr|A0A1E1WVV4|A0A1E1WVV4_TITOB 71.73 26 4 2 Y 9994 Putative potassium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
8 48468 sp|Q0GY43|KIK2_TITDI 62.84 16 3 1 Y 9960 Potassium channel toxin TdiKIK OS=Tityus discrepans OX=57059 PE=1 SV=1
16 55129 tr|A0A7L4XS62|A0A7L4XS62_9SCOR 54.05 23 1 1 Y 8259 Neurotoxin Tpe1 OS=Tityus perijanensis OX=288782 PE=2 SV=1
13 291 sp|P60215|SCX4_TITOB 47.32 13 1 1 Y 10084 Beta-toxin To4 OS=Tityus obscurus OX=1221240 PE=1 SV=2
21 55679 tr|A0A1E1WW03|A0A1E1WW03_TITOB 42.65 13 1 1 Y 9273 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
21 87 sp|C9X4K6|SCNA8_TITDI 42.65 13 1 1 Y 9341 Toxin TdNa8 OS=Tityus discrepans OX=57059 PE=1 SV=1
21 86 sp|H1ZZH8|SCX9_TITOB 42.65 13 1 1 Y 9401 Toxin To9 OS=Tityus obscurus OX=1221240 PE=2 SV=1
15 7789 tr|A0A2E5JHX1|A0A2E5JHX1_9BACT 35.34 2 3 1 N 131824 Uncharacterized protein OS=Halobacteriovorax sp OX=2020862 GN=CME71_04850 PE=4 SV=1
11 46547 tr|A0A1E1WVS3|A0A1E1WVS3_TITOB 30.76 17 1 1 Y 9107 Putative sodium channel toxin OS=Tityus obscurus OX=1221240 PE=4 SV=1
17 7764 tr|A0A2I9LPY0|A0A2I9LPY0_9SCOR 25.10 1 1 1 N 118515 E1 ubiquitin-activating enzyme OS=Centruroides hentzi OX=88313 PE=3 SV=1
17 10616 tr|A0A1W7R9A6|A0A1W7R9A6_9SCOR 25.10 1 1 1 N 117849 E1 ubiquitin-activating enzyme OS=Hadrurus spadix OX=141984 PE=3 SV=1
25 10783 tr|A0A1S5QN50|A0A1S5QN50_TITSE 21.21 1 1 1 N 120694 tubulin-glutamate carboxypeptidase OS=Tityus serrulatus OX=6887 PE=2 SV=1
24 23 tr|A0A1V1WC31|A0A1V1WC31_9SCOR 20.71 1 1 1 N 82960 Putative Neprilysin 1 (Fragment) OS=Superstitionia donensis OX=311983 PE=3 SV=1
20 6473 tr|A0A091FZ00|A0A091FZ00_9AVES 20.28 1 1 1 N 109598 Nesprin-3 (Fragment) OS=Cuculus canorus OX=55661 GN=N303_15526 PE=3 SV=1
20 6187 tr|A0A0A0AAF4|A0A0A0AAF4_CHAVO 20.28 1 1 1 N 108509 Nesprin-3 OS=Charadrius vociferus OX=50402 GN=N301_06983 PE=3 SV=1
20 5004 tr|A0A7L4M3Q5|A0A7L4M3Q5_GLAPT 20.28 1 1 1 N 110315 SYNE3 protein (Fragment) OS=Glareola pratincola OX=43316 GN=Syne3 PE=3 SV=1
20 5003 tr|A0A7K5XBG4|A0A7K5XBG4_9CHAR 20.28 1 1 1 N 110242 SYNE3 protein (Fragment) OS=Dromas ardeola OX=458190 GN=Syne3 PE=3 SV=1
20 6188 tr|A0A7L0RCF5|A0A7L0RCF5_GLABR 20.28 1 1 1 N 110564 SYNE3 protein (Fragment) OS=Glaucidium brasilianum OX=78217 GN=Syne3 PE=3 SV=1
20 6189 tr|A0A8J4N8L4|A0A8J4N8L4_EUDMI 20.28 1 1 1 N 110234 Nesprin-3 (Fragment) OS=Eudyptula minor OX=37083 GN=SYNE3 PE=3 SV=1
20 6163 tr|A0A091TWG2|A0A091TWG2_PHORB 20.28 1 1 1 N 110511 Nesprin-3 OS=Phoenicopterus ruber ruber OX=9218 GN=N337_05074 PE=3 SV=1
20 6203 tr|A0A7K8T3Z3|A0A7K8T3Z3_9AVES 20.28 1 1 1 N 110949 SYNE3 protein (Fragment) OS=Nyctibius bracteatus OX=48426 GN=Syne3 PE=3 SV=1
20 6477 tr|A0A7L0PDU3|A0A7L0PDU3_9AVES 20.28 1 1 1 N 110362 SYNE3 protein (Fragment) OS=Mesembrinibis cayennensis OX=1118748 GN=Syne3 PE=3 SV=1
20 7643 tr|A0A7K7IAP1|A0A7K7IAP1_LOXCU 20.28 1 1 1 N 110240 SYNE3 protein (Fragment) OS=Loxia curvirostra OX=64802 GN=Syne3 PE=3 SV=1
20 6480 tr|A0A7K5T8S0|A0A7K5T8S0_9FRIN 20.28 1 1 1 N 110692 SYNE3 protein (Fragment) OS=Urocynchramus pylzowi OX=571890 GN=Syne3 PE=3 SV=1
total 34 proteins

sp|H1ZZI4|SCX15_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.AGC(+57.02)FFGTNSWC(+57.02)NTEC(+57.02)K.R Y 70.56 1937.7495 111.4 969.9900 2 27.18 866 3 33 48 Carbamidomethylation
K.GYC(+57.02)AWPSC(+57.02)YC(+57.02)YEFTDDSK.I Y 63.93 2307.8547 186.7 1155.1500 2 31.95 1042 2 55 72 Carbamidomethylation
G.GKEGYPLDSSGC(+57.02)K.A Y 63.22 1396.6293 -2.7 699.3200 2 15.23 408 3 20 32 Carbamidomethylation
K.AGC(+57.02)FFGTNSWC(+57.02)NTEC(+57.02)KR.K Y 52.50 2093.8506 3.7 698.9600 3 23.76 734 2 33 49 Carbamidomethylation
K.EGYPLDSSGC(+57.02)K.A Y 41.44 1211.5128 2.2 606.7650 2 17.28 488 1 22 32 Carbamidomethylation
K.IWNAK.T Y 29.63 630.3489 6.0 631.3600 1 12.40 296 2 73 77
G.GM(sub K)EGYPLDSSGC(+57.02)K.A Y 24.47 1399.5748 807.8 701.3600 2 14.98 398 1 20 32 Carbamidomethylation; Mutation
L.DSSGC(+57.02)K.A Y 19.35 652.2487 -9.1 653.2500 1 14.83 392 1 27 32 Carbamidomethylation
total 8 peptides
Best Unique PSM (Scan 866, m/z=969.9900, z=2, RT=27.18, ppm=111.4):
sp|P84631|SCX2_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
KKEGYLVGNDGC(+57.02)K.Y N 80.92 1466.7188 -9.1 734.3600 2 12.80 312 4 1 13 Carbamidomethylation
K.EGYLVGNDGC(+57.02)K.Y N 50.93 1210.5288 377.2 606.5000 2 17.77 506 2 3 13 Carbamidomethylation
KKEGYLVGH(sub N)DGC(+57.02)K.Y N 41.35 1489.7347 -6.2 745.8700 2 11.34 254 2 1 13 Carbamidomethylation; Mutation
C.VH(+143.12)EC(+57.02)ELR.K Y 40.02 1084.5574 -103.2 543.2300 2 18.78 546 1 25 31 Diphthamide; Carbamidomethylation
Y.C(+57.02)VHEC(+57.02)ELR.K Y 34.30 1101.4695 -3.7 551.7400 2 11.95 278 1 24 31 Carbamidomethylation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 9 13 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 6 13 Carbamidomethylation
total 7 peptides
Best Unique PSM (Scan 546, m/z=543.2300, z=2, RT=18.78, ppm=-103.2):
sp|P60214|SCX1_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KKEGYLVGNDGC(+57.02)K.Y N 80.92 1466.7188 -9.1 734.3600 2 12.80 312 4 21 33 Carbamidomethylation
K.EGYLVGNDGC(+57.02)K.Y N 50.93 1210.5288 377.2 606.5000 2 17.77 506 2 23 33 Carbamidomethylation
K.YGC(+57.02)ITRPHQY.C Y 44.16 1293.5924 -5.4 647.8000 2 15.59 422 1 34 43 Carbamidomethylation
C.KKEGYLVGH(sub N)DGC(+57.02)K.Y N 41.35 1489.7347 -6.2 745.8700 2 11.34 254 2 21 33 Carbamidomethylation; Mutation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 29 33 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 26 33 Carbamidomethylation
total 6 peptides
Best Unique PSM (Scan 422, m/z=647.8000, z=2, RT=15.59, ppm=-5.4):
sp|H1ZZI1|SCX12_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.VWDYYNNK.C Y 58.73 1100.4927 -6.6 551.2500 2 20.09 596 6 73 80
A.KEGYPMDHEGC(+57.02)K.F Y 56.43 1449.6017 232.5 725.9766 2 12.30 294 2 20 31 Carbamidomethylation
Y.PMDHEGC(+57.02)K.F Y 35.17 972.3793 -14.3 487.1900 2 12.45 298 1 24 31 Carbamidomethylation
C.FIRPSGFC(+57.02)ER.Y Y 33.63 1267.6132 -6.1 634.8100 2 25.60 806 1 35 44 Carbamidomethylation
F.SC(+57.02)FIRPSGFC(+57.02)ER.Y Y 33.52 1514.6758 1.6 505.9000 3 21.82 662 3 33 44 Carbamidomethylation
S.C(+57.02)FIRPSGFC(+57.02)ER.Y Y 29.92 1427.6438 1.2 714.8300 2 25.70 810 2 34 44 Carbamidomethylation
F.IRPSGFC(+57.02)ER.Y Y 28.52 1120.5447 0.7 561.2800 2 14.53 382 2 36 44 Carbamidomethylation
Y.C(+57.02)YGVPANQK.V Y 23.22 1035.4807 -14.8 518.7400 2 14.07 362 1 64 72 Carbamidomethylation
K.FSC(+57.02)FIRPSGFC(+57.02)ER.Y Y 22.69 1661.7443 423.6 555.1567 3 25.20 792 2 32 44 Carbamidomethylation
A.KEGYPM(+15.99)DHEGC(+57.02)K.F Y 22.23 1465.5966 -7.6 733.8000 2 10.65 226 1 20 31 Oxidation (M); Carbamidomethylation
total 10 peptides
Best Unique PSM (Scan 596, m/z=551.2500, z=2, RT=20.09, ppm=-6.6):
tr|A0A7L4XT36|A0A7L4XT36_TITPA
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
S.KDGYLVGNDGC(+57.02)K.Y N 65.59 1324.6082 -2.0 663.3100 2 14.68 388 3 8 19 Carbamidomethylation
R.PGHYC(+57.02)ASEC(+57.02)SR.V Y 47.10 1322.5132 -5.8 662.2600 2 10.60 224 2 26 36 Carbamidomethylation
S.GSKDGYLVGNDGC(+57.02)K.Y Y 36.47 1468.6616 724.3 735.8700 2 13.05 322 2 6 19 Carbamidomethylation
K.D(+14.02)GYLVGNDGC(+57.02)K.Y N 30.13 1210.5288 402.0 606.5150 2 17.59 500 1 9 19 Methyl ester; Carbamidomethylation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 15 19 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
K.YSC(+57.02)LTR(+28.03)PGHYC(+57.02)ASEC(+57.02)SR.V Y 23.48 2130.9033 -266.4 1066.1750 2 16.45 456 1 20 36 Carbamidomethylation; Dimethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 12 19 Carbamidomethylation
total 7 peptides
Best Unique PSM (Scan 224, m/z=662.2600, z=2, RT=10.60, ppm=-5.8):
tr|A0A7L4XVE5|A0A7L4XVE5_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y N 65.59 1324.6082 -2.0 663.3100 2 14.68 388 3 8 19 Carbamidomethylation
K.YNC(+57.02)LTRPGHYC(+57.02)AS(sub N)EC(+57.02)SR.V Y 43.45 2129.8831 138.5 711.0667 3 16.06 442 1 20 36 Carbamidomethylation; Mutation
K.D(+14.02)GYLVGNDGC(+57.02)K.Y N 30.13 1210.5288 402.0 606.5150 2 17.59 500 1 9 19 Methyl ester; Carbamidomethylation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 15 19 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 12 19 Carbamidomethylation
K.YNC(+57.02)LTRPGHY(-18.01)C(+57.02).A Y 16.37 1421.5969 -711.5 711.3000 2 16.37 452 1 20 30 Carbamidomethylation; Dehydration
total 6 peptides
Best Unique PSM (Scan 442, m/z=711.0667, z=3, RT=16.06, ppm=138.5):
tr|A0A7L4XVB3|A0A7L4XVB3_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y N 65.59 1324.6082 -2.0 663.3100 2 14.68 388 3 8 19 Carbamidomethylation
K.YNC(+57.02)LTRPGHYC(+57.02)AS(sub N)EC(+57.02)SR.V Y 43.45 2129.8831 138.5 711.0667 3 16.06 442 1 20 36 Carbamidomethylation; Mutation
K.D(+14.02)GYLVGNDGC(+57.02)K.Y N 30.13 1210.5288 402.0 606.5150 2 17.59 500 1 9 19 Methyl ester; Carbamidomethylation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 15 19 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 12 19 Carbamidomethylation
K.YNC(+57.02)LTRPGHY(-18.01)C(+57.02).A Y 16.37 1421.5969 -711.5 711.3000 2 16.37 452 1 20 30 Carbamidomethylation; Dehydration
total 6 peptides
Best Unique PSM (Scan 442, m/z=711.0667, z=3, RT=16.06, ppm=138.5):
sp|P60213|SCX3_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y N 65.59 1324.6082 -2.0 663.3100 2 14.68 388 3 21 32 Carbamidomethylation
K.YNC(+57.02)LTRPGHYC(+57.02)AS(sub N)EC(+57.02)SR.V Y 43.45 2129.8831 138.5 711.0667 3 16.06 442 1 33 49 Carbamidomethylation; Mutation
K.D(+14.02)GYLVGNDGC(+57.02)K.Y N 30.13 1210.5288 402.0 606.5150 2 17.59 500 1 22 32 Methyl ester; Carbamidomethylation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 28 32 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 25 32 Carbamidomethylation
K.YNC(+57.02)LTRPGHY(-18.01)C(+57.02).A Y 16.37 1421.5969 -711.5 711.3000 2 16.37 452 1 33 43 Carbamidomethylation; Dehydration
total 6 peptides
Best Unique PSM (Scan 442, m/z=711.0667, z=3, RT=16.06, ppm=138.5):
sp|H1ZZI0|SCX11_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
C.KDGYLVGNDGC(+57.02)K.Y N 65.59 1324.6082 -2.0 663.3100 2 14.68 388 3 21 32 Carbamidomethylation
K.YNC(+57.02)LTRPGHYC(+57.02)AS(sub N)EC(+57.02)SR.V Y 43.45 2129.8831 138.5 711.0667 3 16.06 442 1 33 49 Carbamidomethylation; Mutation
K.D(+14.02)GYLVGNDGC(+57.02)K.Y N 30.13 1210.5288 402.0 606.5150 2 17.59 500 1 22 32 Methyl ester; Carbamidomethylation
G.ND(+57.02)GC(+57.02)K.Y N 27.16 649.2490 -9.6 650.2500 1 17.82 508 1 28 32 Carbamidomethylation (DHKE, X@N-term); Carbamidomethylation
Y.LVGNDGC(+57.02)K.Y N 17.17 861.4014 -0.7 431.7077 2 10.19 208 1 25 32 Carbamidomethylation
K.YNC(+57.02)LTRPGHY(-18.01)C(+57.02).A Y 16.37 1421.5969 -711.5 711.3000 2 16.37 452 1 33 43 Carbamidomethylation; Dehydration
total 6 peptides
Best Unique PSM (Scan 442, m/z=711.0667, z=3, RT=16.06, ppm=138.5):
tr|A0A1E1WVS0|A0A1E1WVS0_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.NSC(+57.02)GLAEENK.C Y 45.01 1120.4818 449.5 561.5000 2 11.72 270 2 36 45 Carbamidomethylation
R.NSC(+57.02)GLAEENKC(+57.02)SIR.G Y 44.01 1636.7297 -14.9 819.3600 2 15.70 426 1 36 49 Carbamidomethylation
R.NVNIIKR.N Y 42.09 855.5290 -27.5 428.7600 2 13.14 326 2 29 35
R.NVNIIK(+14.02).R Y 17.03 713.4435 1232.3 715.3300 1 26.58 844 1 29 34 Methyl ester
total 4 peptides
Best Unique PSM (Scan 270, m/z=561.5000, z=2, RT=11.72, ppm=449.5):
tr|A0A1E1WVV4|A0A1E1WVV4_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.NAIGKC(+57.02)DDFK.C N 40.56 1166.5389 5.6 584.2800 2 14.02 360 1 76 85 Carbamidomethylation
K.LIEVK.D Y 31.27 600.3846 -3.2 601.3900 1 14.27 370 2 39 43
K.CD(+57.02)DFK.C N 28.74 683.2585 -8.4 684.2600 1 6.42 60 1 81 85 Carbamidomethylation (DHKE, X@N-term)
K.C(+57.02)DDFK.C N 24.67 683.2585 6.2 684.2700 1 5.40 18 1 81 85 Carbamidomethylation
K.AGWEKLT(+79.97)S(+79.97)K.S Y 23.79 1178.4774 74.7 590.2900 2 7.47 102 3 48 56 Phosphorylation (STY)
K.NAIGKCD(+57.02)DFK.C N 23.49 1166.5389 5.6 584.2800 2 14.17 366 1 76 85 Carbamidomethylation (DHKE, X@N-term)
K.C(+57.02)DDFK.C N 23.22 683.2585 -8.4 684.2600 1 6.13 48 1 81 85 Carbamidomethylation (DHKE, X@N-term)
total 7 peptides
Best Unique PSM (Scan 370, m/z=601.3900, z=1, RT=14.27, ppm=-3.2):
sp|Q0GY43|KIK2_TITDI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.NAIGKC(+57.02)DDFK.C N 40.56 1166.5389 5.6 584.2800 2 14.02 360 1 76 85 Carbamidomethylation
K.CD(+57.02)DFK.C N 28.74 683.2585 -8.4 684.2600 1 6.42 60 1 81 85 Carbamidomethylation (DHKE, X@N-term)
K.C(+57.02)DDFK.C N 24.67 683.2585 6.2 684.2700 1 5.40 18 1 81 85 Carbamidomethylation
K.AGWD(+14.02)K.L Y 23.73 589.2860 -5.6 590.2900 1 6.73 72 2 48 52 Methyl ester
K.NAIGKCD(+57.02)DFK.C N 23.49 1166.5389 5.6 584.2800 2 14.17 366 1 76 85 Carbamidomethylation (DHKE, X@N-term)
K.C(+57.02)DDFK.C N 23.22 683.2585 -8.4 684.2600 1 6.13 48 1 81 85 Carbamidomethylation (DHKE, X@N-term)
total 6 peptides
Best Unique PSM (Scan 72, m/z=590.2900, z=1, RT=6.73, ppm=-5.6):
tr|A0A7L4XS62|A0A7L4XS62_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.YNC(+57.02)LTRPGTYC(+57.02)ANEC(+57.02)SR.V Y 54.05 2120.8826 -11.5 707.9600 3 18.39 530 1 20 36 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 530, m/z=707.9600, z=3, RT=18.39, ppm=-11.5):
sp|P60215|SCX4_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.KGTFC(+57.02)AEEC(+57.02)TR.M Y 47.32 1357.5754 -7.4 679.7900 2 13.49 340 2 39 49 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 340, m/z=679.7900, z=2, RT=13.49, ppm=-7.4):
tr|A0A1E1WW03|A0A1E1WW03_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Y.C(+57.02)YGLPDKAAIK.G Y 42.65 1234.6379 -10.1 618.3200 2 21.13 636 1 64 74 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 636, m/z=618.3200, z=2, RT=21.13, ppm=-10.1):
sp|C9X4K6|SCNA8_TITDI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Y.C(+57.02)YGLPDKAAIK.G Y 42.65 1234.6379 -10.1 618.3200 2 21.13 636 1 65 75 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 636, m/z=618.3200, z=2, RT=21.13, ppm=-10.1):
sp|H1ZZH8|SCX9_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Y.C(+57.02)YGLPDKAAIK.G Y 42.65 1234.6379 -10.1 618.3200 2 21.13 636 1 65 75 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 636, m/z=618.3200, z=2, RT=21.13, ppm=-10.1):
tr|A0A2E5JHX1|A0A2E5JHX1_9BACT
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
R.EAEIK.K N 22.21 588.3119 443.4 589.5800 1 9.51 182 1 98 102
L.FSDGVNQAISNL.R Y 16.12 1263.6095 724.9 633.2700 2 20.55 614 1 1016 1027
T.ILNIV.F N 15.17 570.3741 -2.4 571.3800 1 29.03 934 1 292 296
total 3 peptides
Best Unique PSM (Scan 614, m/z=633.2700, z=2, RT=20.55, ppm=724.9):
tr|A0A1E1WVS3|A0A1E1WVS3_TITOB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.IYC(+57.02)PNDEVC(+57.02)KGTC(+57.02)K.N Y 30.76 1742.7426 -2.5 581.9200 3 17.15 482 3 34 47 Carbamidomethylation
total 1 peptides
Best Unique PSM (Scan 482, m/z=581.9200, z=3, RT=17.15, ppm=-2.5):
tr|A0A2I9LPY0|A0A2I9LPY0_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.NVILGGVK.S Y 25.10 798.4963 -17.1 799.4900 1 18.91 550 1 91 98
total 1 peptides
Best Unique PSM (Scan 550, m/z=799.4900, z=1, RT=18.91, ppm=-17.1):
tr|A0A1W7R9A6|A0A1W7R9A6_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.NVILGGVK.S Y 25.10 798.4963 -17.1 799.4900 1 18.91 550 1 91 98
total 1 peptides
Best Unique PSM (Scan 550, m/z=799.4900, z=1, RT=18.91, ppm=-17.1):
tr|A0A1S5QN50|A0A1S5QN50_TITSE
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
Q.GGYPETL.I Y 21.21 735.3439 -1.6 736.3500 1 6.93 80 1 118 124
total 1 peptides
Best Unique PSM (Scan 80, m/z=736.3500, z=1, RT=6.93, ppm=-1.6):
tr|A0A1V1WC31|A0A1V1WC31_9SCOR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
K.SYNTLNG.G Y 20.71 767.3450 -886.1 384.3398 2 43.45 1450 1 5 11
total 1 peptides
Best Unique PSM (Scan 1450, m/z=384.3398, z=2, RT=43.45, ppm=-886.1):
tr|A0A091FZ00|A0A091FZ00_9AVES
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 359 363
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A0A0AAF4|A0A0A0AAF4_CHAVO
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 345 349
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7L4M3Q5|A0A7L4M3Q5_GLAPT
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7K5XBG4|A0A7K5XBG4_9CHAR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7L0RCF5|A0A7L0RCF5_GLABR
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A8J4N8L4|A0A8J4N8L4_EUDMI
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A091TWG2|A0A091TWG2_PHORB
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7K8T3Z3|A0A7K8T3Z3_9AVES
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7L0PDU3|A0A7L0PDU3_9AVES
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7K7IAP1|A0A7K7IAP1_LOXCU
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
tr|A0A7K5T8S0|A0A7K5T8S0_9FRIN
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| Protein Coverage | Supporting Peptides | Best Unique PSM |
Protein Coverage:
Supporting Peptides:
Peptide Uniq -10lgP Mass ppm m/z z RT Scan #Spec Start End PTM
P.GEGEK.T Y 20.28 518.2336 17.5 519.2500 1 7.13 88 1 361 365
total 1 peptides
Best Unique PSM (Scan 88, m/z=519.2500, z=1, RT=7.13, ppm=17.5):
 


Prepared with PEAKS ™ (bioinfor.com)