Raw file Experiment Fraction Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS submitted MS/MS submitted (SIL) MS/MS submitted (ISO) MS/MS submitted (PEAK) MS/MS identified MS/MS identified (SIL) MS/MS identified (ISO) MS/MS identified (PEAK) MS/MS identified [%] MS/MS identified (SIL) [%] MS/MS identified (ISO) [%] MS/MS identified (PEAK) [%] Peptide sequences identified Peaks Peaks sequenced Peaks sequenced [%] Peaks repeatedly sequenced Peaks repeatedly sequenced [%] Isotope patterns Isotope patterns sequenced Isotope patterns sequenced (z>1) Isotope patterns sequenced [%] Isotope patterns sequenced (z>1) [%] Isotope patterns repeatedly sequenced Isotope patterns repeatedly sequenced [%] Recalibrated Av. absolute mass deviation [ppm] Mass standard deviation [ppm] Av. absolute mass deviation [mDa] Mass standard deviation [mDa] Label free norm param A1_1 A1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5741 42494 0 49458 35530 0 13928 3274 3164 0 110 6.6 8.9 0 0.79 698 340364 23474 6.9 7532 32 35261 13109 11392 37 38 6827 52 + 0.94756 1.1827 0.69776 0.93761 A1_2 A1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5771 42070 0 48063 36073 0 11990 2698 2598 0 100 5.6 7.2 0 0.83 706 395289 23832 6 7482 31 41511 13786 12049 33 34 6974 51 + 1.4944 1.6898 1.1028 1.2836 A1_3 A1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5703 42323 0 47973 36667 0 11306 4435 4298 0 137 9.2 12 0 1.2 1296 444725 25255 5.7 7455 30 47369 15164 13520 32 33 7498 49 + 1.434 1.6284 1.0303 1.2109 A2_1 A2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5799 42085 0 48470 35696 0 12774 3705 3546 0 159 7.6 9.9 0 1.2 800 353642 23316 6.6 7472 32 36008 13212 11543 37 38 6887 52 + 1.2829 1.4612 0.91532 1.0856 A2_2 A2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5696 42700 0 48473 36927 0 11546 5034 4879 0 155 10 13 0 1.3 1172 397936 24429 6.1 7772 32 41655 14018 12410 34 35 7237 52 + 1.1494 1.3677 0.82845 1.0373 A2_3 A2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5855 41819 0 47111 36525 0 10586 6037 5842 0 195 13 16 0 1.8 1488 422345 23920 5.7 7458 31 44087 14054 12501 32 33 7118 51 + 1.1571 1.3787 0.82655 1.024 A3_1 A3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6026 40416 0 45986 34844 0 11142 4197 4076 0 121 9.1 12 0 1.1 924 365141 24065 6.6 7353 31 38410 14059 12097 37 38 7134 51 + 1.147 1.3512 0.81362 1.0062 A3_2 A3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5723 41739 0 47554 35924 0 11630 4569 4525 0 44 9.6 13 0 0.38 951 381042 23265 6.1 7596 33 39160 13131 11563 34 34 6995 53 + 0.7764 1.0456 0.61321 0.90241 A3_3 A3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5660 42601 0 48252 36948 0 11304 5400 5284 0 116 11 14 0 1 1497 407614 24418 6 7637 31 43813 14361 12733 33 34 7426 52 + 1.2751 1.4702 0.89722 1.0737 F1_1 F1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 8087 29242 0 33246 25232 0 8014 1231 1185 0 46 3.7 4.7 0 0.57 336 398069 19186 4.8 5182 27 41326 12006 9909 29 30 5308 44 + 0.76571 0.96354 0.49138 0.63518 F1_2 F1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6070 36976 0 40336 33608 0 6728 2739 2671 0 68 6.8 7.9 0 1 650 391068 22344 5.7 7012 31 42667 13647 11626 32 36 6903 51 + 1.032 1.2274 0.69791 0.84874 F1_3 F1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6091 36657 0 40357 32951 0 7406 3004 2958 0 46 7.4 9 0 0.62 864 386371 22946 5.9 6822 30 41544 14196 12365 34 36 7088 50 + 0.90496 1.1228 0.62597 0.80851 F2_1 F2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7465 32121 0 36025 28217 0 7808 1754 1684 0 70 4.9 6 0 0.9 378 334822 19147 5.7 6073 32 34609 11079 9284 32 35 5724 52 + 1.2505 1.4395 0.89992 1.0501 F2_2 F2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 12264 10141 0 11506 8772 0 2734 124 119 0 5 1.1 1.4 0 0.18 48 235836 7325 3.1 1550 21 41806 5255 3907 13 14 1634 31 + 0.76105 1.0052 0.36429 0.48122 F2_3 F2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6455 35246 0 37748 32738 0 5010 2066 1993 0 73 5.5 6.1 0 1.5 675 420298 21466 5.1 6834 32 47011 13524 11950 29 30 6838 51 + 1.6398 1.8382 1.2463 1.4367 F3_1 F3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7544 31061 0 34788 27330 0 7458 1460 1411 0 49 4.2 5.2 0 0.66 364 364421 20133 5.5 5713 28 40396 12235 10190 30 33 5772 47 + 1.4594 1.632 1.0471 1.1859 F3_2 F3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7004 33731 0 36798 30662 0 6136 1985 1916 0 69 5.4 6.2 0 1.1 498 370223 19317 5.2 6638 34 38136 11557 10066 30 31 6115 53 + 1.4746 1.6663 1.0808 1.2354 F3_3 F3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7503 30639 0 32931 28347 0 4584 2133 2054 0 79 6.5 7.2 0 1.7 682 405418 18782 4.6 5840 31 40196 11909 10419 30 31 5893 49 + 1.5277 1.709 1.154 1.3164 B1_1 B1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5443 43363 0 50699 36025 0 14674 4210 4074 0 136 8.3 11 0 0.93 805 347931 23641 6.8 8019 34 33913 12699 11073 37 39 6959 55 + 0.90517 1.1339 0.65545 0.88751 B1_2 B1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5719 42712 0 48481 36943 0 11538 5279 5117 0 162 11 14 0 1.4 1221 382792 24226 6.3 7943 33 39784 13858 12418 35 36 7341 53 + 0.83556 1.0945 0.60238 0.84778 B1_3 B1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5853 41188 0 46170 36206 0 9964 4396 4252 0 144 9.5 12 0 1.4 1328 395666 24078 6.1 7564 31 40891 14167 12576 35 36 7383 52 + 1.5324 1.7314 1.0971 1.2848 B2_1 B2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6011 41842 0 46647 37037 0 9610 4884 4830 0 54 10 13 0 0.56 979 371038 23469 6.3 7804 33 37819 13421 12027 35 37 7295 54 + 1.006 1.2225 0.7129 0.92575 B2_2 B2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6008 40676 0 45110 36238 0 8872 6100 5921 0 179 14 16 0 2 1438 404429 24307 6 7436 31 42045 14407 12980 34 36 7342 51 + 1.005 1.2389 0.71216 0.92974 B2_3 B2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6052 39741 0 44654 34828 0 9826 3668 3547 0 121 8.2 10 0 1.2 1312 409829 24891 6.1 7140 29 44614 15243 13625 34 35 7424 49 + 1.6409 1.8259 1.1819 1.3572 B3_1 B3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5950 41246 0 46821 35669 0 11152 3871 3753 0 118 8.3 11 0 1.1 744 349991 22533 6.4 7793 35 34398 12887 11109 37 39 7061 55 + 1.044 1.2366 0.73779 0.93965 B3_2 B3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5943 40891 0 45484 36296 0 9188 3326 3156 0 170 7.3 8.7 0 1.9 958 413510 24869 6 7436 30 42871 14824 13325 35 36 7386 50 + 1.619 1.7989 1.1657 1.3416 B3_3 B3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5951 39441 0 44018 34862 0 9156 4830 4668 0 162 11 13 0 1.8 1387 404189 24882 6.2 7134 29 42564 15242 13534 36 37 7407 49 + 1.0625 1.2799 0.74395 0.93636 C1_1 C1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5869 41268 0 47029 35505 0 11524 4026 3909 0 117 8.6 11 0 1 902 350351 24962 7.1 7549 30 38659 14751 12986 38 39 7486 51 + 1.0788 1.3037 0.75864 0.98953 C1_2 C1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5802 41585 0 46175 36993 0 9182 5126 4963 0 163 11 13 0 1.8 1085 418204 24382 5.8 7928 33 42980 14131 12718 33 34 7441 53 + 1.2037 1.4059 0.84806 1.0392 C1_3 C1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5749 39508 0 43745 35271 0 8474 5338 5171 0 167 12 15 0 2 1523 462923 25937 5.6 7174 28 52142 16597 14945 32 33 7766 47 + 1.3668 1.5625 0.95749 1.14 C2_1 C2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6203 38194 0 43367 33019 0 10348 2656 2515 0 141 6.1 7.6 0 1.4 666 355494 24359 6.9 6911 28 41351 14748 12982 36 37 7084 48 + 1.4393 1.641 1.0123 1.1974 C2_2 C2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 5913 40362 0 44774 35950 0 8824 1148 1045 0 103 2.6 2.9 0 1.2 390 385989 23713 6.1 7610 32 42137 14184 12745 34 35 7355 52 + 2.1405 2.3301 1.7788 1.975 C2_3 C2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6848 36073 0 39851 32291 0 7560 3917 3821 0 96 9.8 12 0 1.3 1175 460805 22963 5 6518 28 50037 14544 12929 29 30 6882 47 + 1.1443 1.3524 0.7758 0.96501 C3_1 C3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6654 35286 0 40104 30468 0 9636 2320 2225 0 95 5.8 7.3 0 0.99 602 353069 22950 6.5 6159 27 38267 13757 11831 36 38 6501 47 + 0.89787 1.0918 0.59834 0.7621 C3_2 C3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6577 36242 0 39771 32707 0 7064 3636 3540 0 96 9.1 11 0 1.4 902 399029 23368 5.9 6761 29 42964 14581 12946 34 36 7040 48 + 1.2164 1.3973 0.84225 0.99541 C3_3 C3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7579 31333 0 34898 27768 0 7130 3576 3475 0 101 10 13 0 1.4 1157 466040 21406 4.6 5314 25 52045 13819 12161 27 27 5954 43 + 1.2928 1.4819 0.91049 1.0773 E1_1 E1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6686 35577 0 41073 30077 0 10996 2272 2209 0 63 5.5 7.3 0 0.57 538 371097 23378 6.3 6534 28 41422 13930 11879 34 35 6619 48 + 1.2707 1.4699 0.87967 1.0477 E1_2 E1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7516 31147 0 35486 26808 0 8678 1698 1652 0 46 4.8 6.2 0 0.53 481 438709 22858 5.2 4281 19 59315 15561 13501 26 28 5065 33 + 1.1642 1.373 0.82962 1.0413 E1_3 E1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6425 35803 0 39045 32557 0 6488 2199 2131 0 68 5.6 6.5 0 1 705 434602 21909 5 6739 31 46877 13858 12067 30 31 6725 49 + 1.5627 1.7502 1.1786 1.3502 E2_1 E2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7762 30740 0 34189 27289 0 6900 1519 1466 0 53 4.4 5.4 0 0.77 411 357742 19589 5.5 5776 29 37014 11951 10092 32 34 5822 49 + 1.4246 1.6086 1.0237 1.1773 E2_2 E2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6822 34543 0 37769 31317 0 6452 2617 2554 0 63 6.9 8.2 0 0.98 613 417455 20631 4.9 6674 32 43997 12704 10988 29 30 6362 50 + 1.3308 1.4892 0.90592 1.0308 E2_3 E2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7476 30827 0 33481 28169 0 5312 2636 2568 0 68 7.9 9.1 0 1.3 805 386438 19824 5.1 5743 29 39810 12614 11007 32 33 6086 48 + 1.203 1.3985 0.86336 1.0365 E3_1 E3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7202 32205 0 35788 28618 0 7170 1723 1679 0 44 4.8 5.9 0 0.61 424 387764 19957 5.1 6370 32 37468 12044 9818 32 36 6107 51 + 1.4112 1.5741 1.0073 1.1425 E3_2 E3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7024 33912 0 37142 30680 0 6462 2682 2604 0 78 7.2 8.5 0 1.2 636 401576 20922 5.2 6458 31 40185 12601 10930 31 33 6353 50 + 1.2842 1.4459 0.89836 1.0286 E3_3 E3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7115 32725 0 35731 29717 0 6014 2472 2432 0 40 6.9 8.2 0 0.67 783 429785 20755 4.8 6321 30 43175 13004 11310 30 31 6428 49 + 1.4159 1.598 1.0609 1.2368 D1_1 D1 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 9132 26366 0 30377 22351 0 8026 1900 1827 0 73 6.3 8.2 0 0.91 420 327033 16937 5.2 4379 26 37844 10485 8701 28 28 4351 41 + 0.88391 1.1237 0.60962 0.8226 D1_2 D1 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7333 33610 0 37526 29692 0 7834 3563 3491 0 72 9.5 12 0 0.92 787 422179 20859 4.9 6023 29 46672 12841 11378 28 28 5843 46 + 1.3404 1.5486 0.96782 1.1462 D1_3 D1 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7164 33543 0 37005 30081 0 6924 4134 4026 0 108 11 13 0 1.6 1274 425230 22137 5.2 5846 26 45870 14054 12334 31 32 6391 45 + 1.3157 1.5219 0.91708 1.1029 D2_1 D2 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7081 35938 0 40309 31563 0 8746 2941 2822 0 119 7.3 8.9 0 1.4 603 381835 21318 5.6 6681 31 39195 12683 10958 32 34 6275 49 + 1.198 1.404 0.8301 1.0171 D2_2 D2 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6882 35037 0 40023 30049 0 9974 3822 3722 0 100 9.5 12 0 1 788 432253 21738 5 6081 28 49694 13391 11773 27 28 5873 44 + 1.3199 1.5161 0.96654 1.1494 D2_3 D2 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6868 34593 0 38681 30501 0 8180 3662 3563 0 99 9.5 12 0 1.2 1105 423820 22651 5.3 5952 26 47458 14217 12525 30 31 6246 44 + 1.4933 1.6853 1.1018 1.2941 D3_1 D3 1 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6818 35888 0 40458 31316 0 9142 2939 2841 0 98 7.3 9.1 0 1.1 591 361682 22024 6.1 6562 30 41153 13484 11352 33 34 6430 48 + 1.0598 1.2675 0.71205 0.88063 D3_2 D3 2 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 6316 37106 0 41435 32773 0 8662 3666 3503 0 163 8.8 11 0 1.9 861 407049 23631 5.8 7076 30 44311 14442 12653 33 34 7043 49 + 1.4258 1.6101 0.98473 1.1483 D3_3 D3 3 Trypsin/P Specific False Oxidation (M);Acetyl (Protein N-term) Carbamidomethyl (C) False False 1 2 Standard 7839 28760 0 32045 25473 0 6572 3137 3048 0 89 9.8 12 0 1.4 971 362360 19789 5.5 5073 26 42125 12657 10813 30 33 5509 44 + 1.2803 1.48 0.93665 1.1194 Total 360042 1963332 0 2200436 1726098 0 474338 177734 172323 0 5411 8.1 10 0 1.1 5103 21114512 2278031 724683 632513 32 33 354006 49 1.2405 1.4605 0.88648 1.0882